Matrix-assisted ionization-ion mobility spectrometry-mass spectrometry: Selective analysis of a europium-PEG complex in a crude mixture
- The analytical utility of a new and simple to use ionization method, matrix-assisted ionization (MAI), coupled with ion mobility spectrometry (IMS) and mass spectrometry (MS) is used to characterize a 2-armed europium(III)-containing poly(ethylene glycol) (Eu-PEG) complex directly from a crude sample. MAI was used with the matrix 1,2-dicyanobenzene, which affords low chemical background relative to matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI). MAI provides high ion abundance of desired products in comparison to ESI and MALDI. Inductively coupled plasma-MS measurements were used to estimate a maximum of 10% of the crude sample by mass was the 2-arm Eu-PEG complex, supporting evidence of selective ionization of Eu-PEG complexes using the new MAI matrix, 1,2-dicyanobenzene. Multiply charged ions formed in MAI enhance the IMS gas-phase separation, especially relative to the singly charged ions observed with MALDI. Individual components are cleanlyThe analytical utility of a new and simple to use ionization method, matrix-assisted ionization (MAI), coupled with ion mobility spectrometry (IMS) and mass spectrometry (MS) is used to characterize a 2-armed europium(III)-containing poly(ethylene glycol) (Eu-PEG) complex directly from a crude sample. MAI was used with the matrix 1,2-dicyanobenzene, which affords low chemical background relative to matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI). MAI provides high ion abundance of desired products in comparison to ESI and MALDI. Inductively coupled plasma-MS measurements were used to estimate a maximum of 10% of the crude sample by mass was the 2-arm Eu-PEG complex, supporting evidence of selective ionization of Eu-PEG complexes using the new MAI matrix, 1,2-dicyanobenzene. Multiply charged ions formed in MAI enhance the IMS gas-phase separation, especially relative to the singly charged ions observed with MALDI. Individual components are cleanly separated and readily identified, allowing characterization of the 2-arm Eu-PEG conjugate from a mixture of the 1-arm Eu-PEG complex and unreacted starting materials. Size-exclusion chromatography, liquid chromatography at critical conditions, MALDI-MS, ESI-MS, and ESI-IMS-MS had difficulties with this analysis, or failed.…
Autor*innen: | J.L. Fischer, C.A. Lutomski, T.J. El-Baba, B.N. Siriwardena-Mahanama, Steffen WeidnerORCiD, Jana FalkenhagenORCiD, M. J. Allen, S. Trimpin |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Journal of the American society for mass spectrometry |
Jahr der Erstveröffentlichung: | 2015 |
Herausgeber (Institution): | American Society for Mass Spectrometry |
Verlag: | Elsevier |
Verlagsort: | New York, NY |
Jahrgang/Band: | 26 |
Ausgabe/Heft: | 12 |
Erste Seite: | 2086 |
Letzte Seite: | 2095 |
Freie Schlagwörter: | ESI; Electrospray ionization; Europium; Inductively coupled plasma-mass spectrometry; Liquid chromatography at critical conditions; MALDI; Matrix-assisted ionization ion mobility spectrometry mass spectrometry; Matrix-assisted laser desorption/ionization; Poly(ethylene glycol); SEC; Size-exclusion chromatography |
DOI: | 10.1007/s13361-015-1233-8 |
ISSN: | 1044-0305 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 20.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 25.01.2016 |