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Separation and quantification of silver nanoparticles and silver ions using reversed phase high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry in combination with isotope dilution analysis

  • A reversed phase high performance liquid chromatography coupled to an inductively coupled Plasma mass spectrometer (HPLC–ICP-MS) approach in combination with isotope dilution analysis (IDA) for the separation and parallel quantification of nanostructured and ionic silver (Ag) is presented. The main Focus of this work was the determination of the ionic Ag concentration. For a sufficient stabilization of the Ions without dissolving the nanoparticles (NPs), the eluent had to be initially optimized. The determined Ag ion concentration was in a good agreement with results obtained using ultrafiltration. Further, the mechanism of the NP separation in the HPLC column was investigated. Typical size exclusion effects were found by comparing results from columns with different pore sizes. Since the recovery rates decreased with increasing Ag NP size and large Ag NPs did not elute from the column, additional interactions of the particles with the stationary phase were assumed. Our results revealA reversed phase high performance liquid chromatography coupled to an inductively coupled Plasma mass spectrometer (HPLC–ICP-MS) approach in combination with isotope dilution analysis (IDA) for the separation and parallel quantification of nanostructured and ionic silver (Ag) is presented. The main Focus of this work was the determination of the ionic Ag concentration. For a sufficient stabilization of the Ions without dissolving the nanoparticles (NPs), the eluent had to be initially optimized. The determined Ag ion concentration was in a good agreement with results obtained using ultrafiltration. Further, the mechanism of the NP separation in the HPLC column was investigated. Typical size exclusion effects were found by comparing results from columns with different pore sizes. Since the recovery rates decreased with increasing Ag NP size and large Ag NPs did not elute from the column, additional interactions of the particles with the stationary phase were assumed. Our results reveal that the presented method is not only applicable to Ag NPs, but also to gold and polystyrene NPs. Finally, IDA-HPLC-ICP-MS experiments in single particle mode were performed to determine the particle cut-off size. The comparison with conventional spICP-MS experiments resulted in a similar diameter and particle size distribution.zeige mehrzeige weniger

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Metadaten
Autoren/innen:Carina A. Sötebier, Steffen Weidner, Norbert Jakubowski, Ulrich Panne, J. Bettmer
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Journal of Chromatography A
Jahr der Erstveröffentlichung:2016
Organisationseinheit der BAM:P Präsidium
1 Analytische Chemie; Referenzmaterialien
P Präsidium / P.0 Präsident und Vizepräsident
1 Analytische Chemie; Referenzmaterialien / 1.0 Abteilungsleitung und andere
Jahrgang/Band:1468
Erste Seite:102
Letzte Seite:108
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten
Freie Schlagwörter:High performance liquid chromatography; ICP-MS; Isotope dilution analysis; Silver nanoparticles; Speciation
Themenfelder/Aktivitätsfelder der BAM:Material
Analytical Sciences
DOI:https://doi.org/10.1016/j.chroma.2016.09.028
URL:http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord;UT=WOS:000385326500014
ISSN:0021-9673
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:05.10.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:10.11.2016