Detection limits of matrix-assisted laser desorption/ionisation mass spectrometry coupled to chromatography - a new application of solvent-free sample preparation
- The detection limits of matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) by semi-online coupling with chromatography were investigated using various mixtures of polyethylene oxides (PEOs) with different end groups. In contrast to the common dried-droplet sample preparation technique, which results in an inhomogeneous sample-to-matrix ratio within the MALDI spot, the used coupling technique offers a very high reproducibility combined with surpassing sensitivity of a few femtograms over a broad range of sample-to-matrix ratios. These results are in correlation with the results of the recently established solvent-free MALDI-TOFMS method utilising the grinding approach and are also of assistance towards the more theoretical aspect of MALDI that suggests that there is no necessity for an analyte incorporation into a matrix crystal for excellent matrix-assistance.
Autor*innen: | Jana FalkenhagenORCiD, Steffen WeidnerORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Rapid communications in mass spectrometry |
Jahr der Erstveröffentlichung: | 2005 |
Verlag: | Wiley |
Verlagsort: | Chichester |
Jahrgang/Band: | 19 |
Ausgabe/Heft: | 24 |
Erste Seite: | 3724 |
Letzte Seite: | 3730 |
DOI: | 10.1002/rcm.2256 |
ISSN: | 0951-4198 |
ISSN: | 1097-0231 |
Verfügbarkeit des Dokuments: | Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access") |
Bibliotheksstandort: | Sonderstandort: Publica-Schrank |
Datum der Freischaltung: | 19.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 16.01.2006 |