High repetition rate atmospheric pressure matrix-assisted laser desorption/ionization in combination with liquid matrices
- One major drawback of matrix-assisted laser desorption/ionization (MALDI) is still the relatively poor pulse-to-pulse reproducibility of the signal intensity. This problem, caused by insufficient homogeneity in the matrix/analyte co-crystallization, is usually circumvented by averaging the detected ion intensity over several shots. However, during the consecutive laser pulses, the applied matrix gets depleted and only a number of subsequent experiments can be done on the same sample spot. In order to achieve the desired long-term stability in combination with a sufficient pulse-to-pulse reproducibility, recently liquid MALDI matrices have been introduced. This contribution demonstrates the promising combination of liquid matrices with high repetition rate lasers for atmospheric pressure MALDI (AP-MALDI). To demonstrate the robustness of the new approach, two different kinds of liquid matrices were used in combination with both a typical flashlamp pumped 15 Hz laser and a diode pumpedOne major drawback of matrix-assisted laser desorption/ionization (MALDI) is still the relatively poor pulse-to-pulse reproducibility of the signal intensity. This problem, caused by insufficient homogeneity in the matrix/analyte co-crystallization, is usually circumvented by averaging the detected ion intensity over several shots. However, during the consecutive laser pulses, the applied matrix gets depleted and only a number of subsequent experiments can be done on the same sample spot. In order to achieve the desired long-term stability in combination with a sufficient pulse-to-pulse reproducibility, recently liquid MALDI matrices have been introduced. This contribution demonstrates the promising combination of liquid matrices with high repetition rate lasers for atmospheric pressure MALDI (AP-MALDI). To demonstrate the robustness of the new approach, two different kinds of liquid matrices were used in combination with both a typical flashlamp pumped 15 Hz laser and a diode pumped solid state laser operated at 5 kHz. The latter showed a stable ion signal over more than 3,500,000 consecutive laser pulses.…
Autor*innen: | Andreas Bierstedt, Arne Stindt, Carsten Warschat, Ulrich PanneORCiD, Jens RiedelORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | European journal of mass spectrometry |
Jahr der Erstveröffentlichung: | 2014 |
Verlag: | IM Publications |
Verlagsort: | Chichester |
Jahrgang/Band: | 20 |
Ausgabe/Heft: | 5 |
Erste Seite: | 367 |
Letzte Seite: | 374 |
Freie Schlagwörter: | AP-MALDI; DPSS laser; High repetition rate; Ionic liquid; Liquid matrix |
DOI: | 10.1255/ejms.1292 |
ISSN: | 1469-0667 |
ISSN: | 1356-1049 |
ISSN: | 1365-0718 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 20.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 09.02.2015 |