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The toolbox of fluorescence standards: Flexible calibration tools for the standardization of fluorescence-based measurements

  • To improve the reliability of fluorescence data in the life and material sciences and to enable accreditation of fluorescence techniques, standardization concepts are required that guarantee and improve the comparability of fluorescence measurements. At the core of such concepts are simple and evaluated fluorescence standards for the consideration of instrument-specific spectral and intensity distortions of measured signals and for instrument performance validation (IPV). Similarly in need are fluorescence intensity standards for the quantification from measured intensities and for signal referencing, thereby accounting for excitation light-induced intensity fluctuations. These standards should be preferably certified, especially for use in regulated areas like medical diagnostics. This encouraged us to develop liquid and solid standards for different fluorescence parameters and techniques for use under routine measurement conditions in different formates. Special emphasis wasTo improve the reliability of fluorescence data in the life and material sciences and to enable accreditation of fluorescence techniques, standardization concepts are required that guarantee and improve the comparability of fluorescence measurements. At the core of such concepts are simple and evaluated fluorescence standards for the consideration of instrument-specific spectral and intensity distortions of measured signals and for instrument performance validation (IPV). Similarly in need are fluorescence intensity standards for the quantification from measured intensities and for signal referencing, thereby accounting for excitation light-induced intensity fluctuations. These standards should be preferably certified, especially for use in regulated areas like medical diagnostics. This encouraged us to develop liquid and solid standards for different fluorescence parameters and techniques for use under routine measurement conditions in different formates. Special emphasis was dedicated to the determination and control of the spectral responsivity of detection systems, wavelength accuracy, homogeneity of illumination, and intensity referencing for e.g. spectrofluorometers, fluorescence sensors and confocal laser scanning fluorescence microscopes. Here, we will present design concepts and examples for mono- and multifunctional fluorescence standards that provide traceability to radiometric units and present a first step towards a toolbox of standards.zeige mehrzeige weniger

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Metadaten
Autor*innen:Ute Resch-GengerORCiD, Katrin HoffmannORCiD, Christian WürthORCiD, Thomas Behnke, Angelika Hoffmann, Dietmar Pfeifer, A. Engel
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Proceedings / SPIE : P
Jahr der Erstveröffentlichung:2010
Herausgeber (Institution):Society of Photo-Optical Instrumentation Engineers
Verlag:Soc.
Verlagsort:Redondo Beach, Calif. [u.a.]
Jahrgang/Band:7666
Ausgabe/Heft:76661J
Erste Seite:1
Letzte Seite:12
Freie Schlagwörter:Calibration tool; Fluorescence; Fluorescence standard; Glass; Instrument performance validation; Intensity standard; Liquid standard; Quality assurance; Spectral fluorescence standard; Traceability
DOI:10.1117/12.853133
ISSN:0277-786X
ISSN:0038-7355
ISSN:0361-0748
Verfügbarkeit des Dokuments:Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
Bibliotheksstandort:Sonderstandort: Publica-Schrank
Datum der Freischaltung:19.02.2016
Referierte Publikation:Nein
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