Zitieren Sie bitte immer diesen URN: urn:nbn:de:kobv:b43-505772
Ectoine interaction with DNA: Influence on ultraviolet radiation damage
- Ectoine is a small zwitterionic osmolyte and compatible solute, which does not interfere with cell metabolism even at molar concentrations. Plasmid DNA (pUC19) was irradiated with ultraviolet radiation (UV-C at 266 nm) under quasi physiological conditions (PBS) and in pure water in the presence and absence of ectoine (THP(B)) and hydroxyectoine (THP(A)). Different types of UV induced DNA damage were analysed: DNA single-strand breaks (SSBs), abasic sites and cyclobutane pyrimidine dimers (CPDs). A complex interplay between these factors was observed with respect to the nature and occurrence of DNA damage with 266 nm photons. In PBS, the cosolutes showed efficient protection against base damage, whilst in pure water, a dramatic shift from SSB damage to base damage was observed when cosolutes were added. To test whether these effects are caused by ectoine binding to DNA, further experiments were conducted: small-angle X-ray scattering (SAXS), surface-plasmon resonance (SPR) measurementsEctoine is a small zwitterionic osmolyte and compatible solute, which does not interfere with cell metabolism even at molar concentrations. Plasmid DNA (pUC19) was irradiated with ultraviolet radiation (UV-C at 266 nm) under quasi physiological conditions (PBS) and in pure water in the presence and absence of ectoine (THP(B)) and hydroxyectoine (THP(A)). Different types of UV induced DNA damage were analysed: DNA single-strand breaks (SSBs), abasic sites and cyclobutane pyrimidine dimers (CPDs). A complex interplay between these factors was observed with respect to the nature and occurrence of DNA damage with 266 nm photons. In PBS, the cosolutes showed efficient protection against base damage, whilst in pure water, a dramatic shift from SSB damage to base damage was observed when cosolutes were added. To test whether these effects are caused by ectoine binding to DNA, further experiments were conducted: small-angle X-ray scattering (SAXS), surface-plasmon resonance (SPR) measurements and Raman spectroscopy. The results show, for the first time, a close interaction between ectoine and DNA. This is in stark contrast to the assumption made by preferential exclusion models, which are often used to interpret the behaviour of compatible solutes within cells and with biomolecules. It is tentatively proposed that the alterations of UV damage to DNA are attributed to ectoine influence on nucleobases through the direct interaction between ectoine and DNA.…
Autor*innen: | Marc Benjamin HahnORCiD, Glen Jacob SmalesORCiD, H. Seitz, Tihomir Solomun, Heinz SturmORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Physical Chemistry Chemical Physics |
Jahr der Erstveröffentlichung: | 2020 |
Organisationseinheit der BAM: | 6 Materialchemie |
6 Materialchemie / 6.5 Synthese und Streuverfahren nanostrukturierter Materialien | |
6 Materialchemie / 6.6 Physik und chemische Analytik der Polymere | |
Veröffentlichende Institution: | Bundesanstalt für Materialforschung und -prüfung (BAM) |
Verlag: | Royal Society of Chemistry |
Verlagsort: | Cambridge |
Jahrgang/Band: | 22 |
Ausgabe/Heft: | 13 |
Erste Seite: | 6984 |
Letzte Seite: | 6992 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik | |
Freie Schlagwörter: | 266nm; Abasic site; Agarose gel electrophorese; CPD; Cancer; Compatible solute; Counterions; DNA; DNA base damage; DNA damage; DNA melting temperature; DNA protection; DNA strand-break; Ectoine; Ectoine DNA protection; Ectoine UV absorption; Ectoine-DNA binding; Excited states; Hydroxyectoine; Hydroxyl radicals; OH scavenger; PBS; Preferential exclusion; Radiation damage; Radiation protection; Radical scavenger; Raman spectroscopy; SSB; SYBR gold; Salt; Sunscreen; Therapy; UV absorption; UV irradiation; UV photons; UV protection; UV-A; UV-B; UV-C; UV-Vis; Zwitterion |
Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
Umwelt | |
Umwelt / Umwelt-Material-Interaktionen | |
DOI: | 10.1039/d0cp00092b |
URN: | urn:nbn:de:kobv:b43-505772 |
ISSN: | 1463-9076 |
ISSN: | 1463-9084 |
Verfügbarkeit des Dokuments: | Datei für die Öffentlichkeit verfügbar ("Open Access") |
Lizenz (Deutsch): | Creative Commons - CC BY - Namensnennung 4.0 International |
Datum der Freischaltung: | 23.03.2020 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 30.03.2020 |
Schriftenreihen ohne Nummerierung: | Wissenschaftliche Artikel der BAM |