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Preparation of polystyrene@silica core-shell particles for suspension arrays

  • Suspension arrays play a major role in recent developments of multiplexed proteomic and genomic assays. Using for instance cytometry, this microparticle-based analytical method features several advantages compared with flat array technologies such as facile fabrication of the analytical platform, high analytical surface area, fast reaction kinetics and a high analytical throughput. Since the first immunoassay for soluble antigens employing flow cytometry in 1982 by Lisi et al., enormous improvements were made concerning the assay chemistry and multiplexing capabilities of this technique. More recently, bead-based applications in microfluidic devices were studied which show promising abilities in point-of-need testing technologies. However, for optimum performance highly mono-disperse and low-density particles are required to guarantee proper correlation and to prevent sedimentation. Usually polymeric particles, such as polystyrene and PMMA spheres, fulfill these requirements and offerSuspension arrays play a major role in recent developments of multiplexed proteomic and genomic assays. Using for instance cytometry, this microparticle-based analytical method features several advantages compared with flat array technologies such as facile fabrication of the analytical platform, high analytical surface area, fast reaction kinetics and a high analytical throughput. Since the first immunoassay for soluble antigens employing flow cytometry in 1982 by Lisi et al., enormous improvements were made concerning the assay chemistry and multiplexing capabilities of this technique. More recently, bead-based applications in microfluidic devices were studied which show promising abilities in point-of-need testing technologies. However, for optimum performance highly mono-disperse and low-density particles are required to guarantee proper correlation and to prevent sedimentation. Usually polymeric particles, such as polystyrene and PMMA spheres, fulfill these requirements and offer good accessibility in conventionally equipped laboratories. Major limitations of these beads however reside with the possibilities for post-modification of the surface and the sensitivity of the polymeric network to the presence of (traces of) organic solvents. A way to circumvent these issues while retaining the key requirements on applications in suspension arrays is the coating of the polymer particles with a silica shell. This strategy not only increases the thermal and chemical stability but facilitates further tailoring of the surface properties with a large variety of commercially available silanes. This contribution presents a facile synthetic approach to highly mono-disperse polystyrene@silica core-shell particles, bearing simple means to control the size of the particles and the silica shell thickness. Main parameters such as surface area and shell structure are discussed in correlation to analytical benefits of the particles. For the latter, standard flow cytometry applications with (bio)analytical suspension assays are performed.zeige mehrzeige weniger

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Metadaten
Autor*innen:Dominik Sarma
Koautor*innen:Knut Rurack
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Deutsch
Titel des übergeordneten Werkes (Deutsch):4th International Colloids Conference: Surface Design & Engineering
Jahr der Erstveröffentlichung:2014
Veranstaltung:4th International Colloids Conference: Surface Design & Engineering
Veranstaltungsort:Madrid, Spain
Beginndatum der Veranstaltung:2014-06-15
Enddatum der Veranstaltung:2014-06-18
Verfügbarkeit des Dokuments:Weder Datei noch physisches Exemplar vorhanden ("No Access")
Datum der Freischaltung:20.02.2016
Referierte Publikation:Nein
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