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Simple approaches to fluorescence lifetime standards using dye-quencher pairs

  • Photoluminescence techniques are amongst the most widely used tools in the material and life sciences, with new and exciting applications continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. Increasing applications of fluorescence techniques in the life sciences and emerging medical applications of fluorescence microscopic techniques including 1P and 2P fluorescence microscopy combined with fluorescence lifetime imaging (FLIM) in e.g. in vivo eye diagnostics boosted the demand for robust, easy-to-use, and reliable fluorescence standards to ensure the reliability and comparability of fluorescence data. This includes fluorescence standards for the consideration of instrument-specific spectral and intensity distortions of measured signals and instrument performance validation, fluorescence intensity standards for the quantification of measured intensitiesPhotoluminescence techniques are amongst the most widely used tools in the material and life sciences, with new and exciting applications continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. Increasing applications of fluorescence techniques in the life sciences and emerging medical applications of fluorescence microscopic techniques including 1P and 2P fluorescence microscopy combined with fluorescence lifetime imaging (FLIM) in e.g. in vivo eye diagnostics boosted the demand for robust, easy-to-use, and reliable fluorescence standards to ensure the reliability and comparability of fluorescence data. This includes fluorescence standards for the consideration of instrument-specific spectral and intensity distortions of measured signals and instrument performance validation, fluorescence intensity standards for the quantification of measured intensities and for signal referencing, and lately, also fluorescence lifetime standards.zeige mehrzeige weniger

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Metadaten
Autoren/innen:Thomas Behnke, Eva-Maria Laux, Katrin Hoffmann, S. Peters, J. Haueisen, M. Klemm, Ute Resch-Genger
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Biomedizinische Technik = Biomedical engineering
Jahr der Erstveröffentlichung:2012
Herausgeber (Institution):Österreichische Gesellschaft für Biomedizinische Technik
Verlag:De Gruyter
Verlagsort:Berlin
Jahrgang/Band:57
Ausgabe/Heft:Suppl. 1
Erste Seite:613
Veranstaltung:BMT 2012 - 46. DGBMT Jahrestagung
Veranstaltungsort:Jena, Germany
Beginndatum der Veranstaltung:16.09.2012
Enddatum der Veranstaltung:19.09.2012
DOI:https://doi.org/10.1515/bmt-2012-4499
ISSN:0013-5585
ISSN:1862-278X
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:20.02.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:14.02.2013