Standardization of fluorescence measurements in the UV/vis/NIR/IR
- Photoluminescence techniques are amongst the most widely used Tools in the life sciences, with new and exciting applications in medical diagnostics and molecular Imaging continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for Multiplexing, remote sensing, and miniaturization. General drawbacks are, however, signals, that contain unwanted wavelength- and polarization contributions from Instrument-dependent effects, which are also time-dependent due to aging of Instrument-components, and difficulties to measure absolute flourescence entensities. Moreover, scattering Systems require Special measurement geometries and the interest in new optical Reporters with Emission > 1000 nm strategies for reliable measurements in the second diagnostic for the comparison of material Performance and the rational designg of new flourophores with improved properties. Here, we present strategies to versatile method-adaptablePhotoluminescence techniques are amongst the most widely used Tools in the life sciences, with new and exciting applications in medical diagnostics and molecular Imaging continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for Multiplexing, remote sensing, and miniaturization. General drawbacks are, however, signals, that contain unwanted wavelength- and polarization contributions from Instrument-dependent effects, which are also time-dependent due to aging of Instrument-components, and difficulties to measure absolute flourescence entensities. Moreover, scattering Systems require Special measurement geometries and the interest in new optical Reporters with Emission > 1000 nm strategies for reliable measurements in the second diagnostic for the comparison of material Performance and the rational designg of new flourophores with improved properties. Here, we present strategies to versatile method-adaptable liquid and solid flourescence Standards for different flourescence paramters including traceable Instrument calibration procedures and the design of integrating spere setups for the absolute measurements of emission spectra and Quantum yields in the wavelength Region of 350 to 1600 nm. Examples are multi-Emitter glasses, spectral flourescence Standards, and quantum yield Standards for the UV/vis/NIR.…
Autor*innen: | Jutta Pauli, Katrin HoffmannORCiD, Christian WürthORCiD, Thomas Behnke, Ute Resch-GengerORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Standardization of Fluorescence Measurements in the UV/vis/NIR/IR |
Jahr der Erstveröffentlichung: | 2017 |
Verlag: | Proceedings of SPIE |
Jahrgang/Band: | 10049 |
Erste Seite: | 1 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten | |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik | |
Freie Schlagwörter: | Absolute flourometry; Calibration; Emission standards; Fluorescence; IR; Integrating sphere spectroscopy; NIR; Nanoparticle; Quantum yield standard; Reference material; Standard |
Veranstaltung: | Conference on Molecular-Guided Surgery - Molecules, Devices, and Applications III |
Veranstaltungsort: | San Francisco, CA, USA |
Beginndatum der Veranstaltung: | 28.01.2017 |
Enddatum der Veranstaltung: | 30.01.2017 |
DOI: | 10.1117/12.2255728 |
ISSN: | 0277-786X |
ISBN: | 978-1-5106-0539-8 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 06.11.2017 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 06.11.2017 |