Encapsulation of hydrophobic dyes in polystyrene micro- and nanoparticles via swelling procedures
- Aiming at the derivation of a generalized procedure for the straightforward preparation of particles fluorescing in the visible and near-infrared (NIR) spectral region, different swelling procedures for the loading of the hydrophobic polarity-probe Nile Red into nano- and micrometer sized polystyrene particles were studied and compared with respect to the optical properties of the resulting particles. The effect of the amount of incorporated dye on the spectroscopic properties of the particles was investigated for differently sized beads with different surface chemistries, i.e., non-functionalized, aminomodified and PEG-grafted surfaces. Moreover, photostability and leaking studies were performed. The main criterion for the optimization of the dye loading procedures was a high and thermally and photochemically stable fluorescence output of the particles for the future application of these systems as fluorescent labels.
Autor*innen: | Thomas Behnke, Christian WürthORCiD, Katrin HoffmannORCiD, Martin Hübner, Ulrich PanneORCiD, Ute Resch-GengerORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Journal of fluorescence |
Jahr der Erstveröffentlichung: | 2011 |
Verlag: | Plenum Publ. Corp. |
Verlagsort: | New York, NY |
Jahrgang/Band: | 21 |
Ausgabe/Heft: | 3 |
Erste Seite: | 937 |
Letzte Seite: | 944 |
Freie Schlagwörter: | Encapsulation; Fluorescence; Microparticles; Nanoparticles; Nile red; Polystyrene; Swelling |
DOI: | 10.1007/s10895-010-0632-2 |
ISSN: | 1053-0509 |
ISSN: | 1573-4994 |
Verfügbarkeit des Dokuments: | Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access") |
Bibliotheksstandort: | Sonderstandort: Publica-Schrank |
Datum der Freischaltung: | 19.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 09.12.2010 |