Das Suchergebnis hat sich seit Ihrer Suchanfrage verändert. Eventuell werden Dokumente in anderer Reihenfolge angezeigt.
  • Treffer 10 von 12
Zurück zur Trefferliste

Traceability in Fluorometry: Part II. Spectral Fluorescence Standards

  • The need for the traceable characterization of fluorescence instruments is emphasized from a chemist’s point of view, focusing on spectral fluorescence standards for the determination of the wavelength- and polarization-dependent relative spectral responsivity and relative spectral irradiance of fluorescence measuring systems, respectively. In a first step, major sources of error of fluorescence measurements and instrument calibration are revealed to underline the importance of this issue and to illustrate advantages and disadvantages of physical and chemical transfer standards for generation of spectral correction curves. Secondly, examples for sets of traceable chemical emission and excitation standards are shown that cover a broad spectral region and simple procedures for the determination of corrected emission spectra with acceptable uncertainties are presented. With proper consideration of the respective measurement principle and geometry, these dye-based characterizationThe need for the traceable characterization of fluorescence instruments is emphasized from a chemist’s point of view, focusing on spectral fluorescence standards for the determination of the wavelength- and polarization-dependent relative spectral responsivity and relative spectral irradiance of fluorescence measuring systems, respectively. In a first step, major sources of error of fluorescence measurements and instrument calibration are revealed to underline the importance of this issue and to illustrate advantages and disadvantages of physical and chemical transfer standards for generation of spectral correction curves. Secondly, examples for sets of traceable chemical emission and excitation standards are shown that cover a broad spectral region and simple procedures for the determination of corrected emission spectra with acceptable uncertainties are presented. With proper consideration of the respective measurement principle and geometry, these dye-based characterization procedures can be not only applied to spectrofluorometers but also to other types of fluorescence measuring systems and even to Raman spectrometers.zeige mehrzeige weniger

Metadaten exportieren

Weitere Dienste

Teilen auf Twitter Suche bei Google Scholar
Metadaten
Autoren/innen:Ute Resch-GengerORCiD, Dietmar Pfeifer, Christian Monte, Walter Pilz, Angelika Hoffmann, Monika Spieles, Knut Rurack, J. Hollandt, D. Taubert, B. Schönenberger, P. Nording
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Journal of fluorescence
Jahr der Erstveröffentlichung:2005
Verlag:Plenum Publ. Corp.
Verlagsort:New York, NY
Jahrgang/Band:15
Ausgabe/Heft:3
Erste Seite:315
Letzte Seite:336
Freie Schlagwörter:Emission; Excitation; Fluorescence; Spectral correction; Standard; Traceability
DOI:https://doi.org/10.1007/s10895-005-2629-9
ISSN:1053-0509
ISSN:1573-4994
Verfügbarkeit des Dokuments:Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
Bibliotheksstandort:Sonderstandort: Publica-Schrank
Datum der Freischaltung:19.02.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:05.09.2005