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Measurements and simulations of microscopic damage to DNA in water by 30 keV electrons: A general approach applicable to other radiation sources and biological targets

  • The determination of the microscopic dose-damage relationship for DNA in an aqueous environment is of a fundamental interest for dosimetry and applications in radiation therapy and protection. We combine geant4 particle-scattering simulations in water with calculations concerning the movement of biomolecules to obtain the energy deposit in the biologically relevant nanoscopic volume. We juxtaposition these results to the experimentally determined damage to obtain the dose-damage relationship at a molecular level. This approach is tested for an experimentally challenging system concerning the direct irradiation of plasmid DNA (pUC19) in water with electrons as primary particles. Here a microscopic target model for the plasmid DNA based on the relation of lineal energy and radiation quality is used to calculate the effective target volume. It was found that on average fewer than two ionizations within a 7.5-nm radius around the sugar-phosphate backbone are sufficient to cause a singleThe determination of the microscopic dose-damage relationship for DNA in an aqueous environment is of a fundamental interest for dosimetry and applications in radiation therapy and protection. We combine geant4 particle-scattering simulations in water with calculations concerning the movement of biomolecules to obtain the energy deposit in the biologically relevant nanoscopic volume. We juxtaposition these results to the experimentally determined damage to obtain the dose-damage relationship at a molecular level. This approach is tested for an experimentally challenging system concerning the direct irradiation of plasmid DNA (pUC19) in water with electrons as primary particles. Here a microscopic target model for the plasmid DNA based on the relation of lineal energy and radiation quality is used to calculate the effective target volume. It was found that on average fewer than two ionizations within a 7.5-nm radius around the sugar-phosphate backbone are sufficient to cause a single strand break, with a corresponding median lethal energy deposit being E1/2=6±4 eV. The presented method is applicable for ionizing radiation (e.g., γ rays, x rays, and electrons) and a variety of targets, such as DNA, proteins, or cells.zeige mehrzeige weniger
Metadaten
Autoren/innen:Marc Benjamin Hahn, Susann Meyer, Hans-Joerg Kunte, Tihomir Solomun, Heinz Sturm
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Physical Review E
Jahr der Erstveröffentlichung:2017
Organisationseinheit der BAM:4 Material und Umwelt
6 Materialschutz und Oberflächentechnik
6 Materialschutz und Oberflächentechnik / 6.6 Nano-Tribologie und Nanostrukturierung von Oberflächen
4 Material und Umwelt / 4.0 Abteilungsleitung und andere
Veröffentlichende Institution:Bundesanstalt für Materialforschung und -prüfung (BAM)
Herausgeber (Institution):American Physical Society
Verlag:American Physical Society
Verlagsort:USA
Jahrgang/Band:95
Ausgabe/Heft:5
Erste Seite:052419-1
Letzte Seite:052419-8
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik
Freie Schlagwörter:DNA; DNA radiation damage; Dosimetry; Double-strand break (DSB); Geant4; Lethal dose; Low energy electrons; Microdosimetry; Monte Carlo simulation; Plasmid DNA in water; Radiation damage; Radiation damage to biomolecules; Single-strand break (SSB); Solutions (pH, salinity, cosolutes)
Themenfelder/Aktivitätsfelder der BAM:Umwelt
Analytical Sciences
Analytical Sciences / Spurenanalytik und chemische Zusammensetzung
DOI:https://doi.org/10.1103/PhysRevE.95.052419
URN:urn:nbn:de:kobv:b43-404244
URL:http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord;UT=WOS:000402477200012
ISSN:2470-0045
ISSN:2470-0053
Verfügbarkeit des Volltexts:Volltext-PDF für die Öffentlichkeit verfügbar ("Open Access")
Lizenz (Deutsch):License LogoCreative Commons - Namensnennung
Datum der Freischaltung:31.05.2017
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:22.06.2017
Schriftenreihen ohne Nummerierung:Wissenschaftliche Artikel der BAM