Click chemistry on silicon nitride for biosensor fabrication
- Biosensors are of essential importance in medical and biological diagnostics. Often, they are produced using silane chemistry on glass or silicon oxide surfaces. However, controlling that silane chemistry is challenging. Here, we present an alternative strategy to form functional organic layers and biosensors on silicon Nitride (Si3N4). H-terminated Si3N4 films are used to generate reactive azide groups by various azidation methods. Biomolecular probes can then be immobilized using click chemistry reactions with the azide groups and due to its high sensitivity in XPS a fluorine-substituted test alkyne was utilized to optimize click chemistry conditions. After that a biotinylated alkyne was clicked to Si3N4 surfaces followed by immobilization of streptavidin as analyte in a model assay. The functionalized surfaces were thoroughly characterized by surface chemical analysis using X-ray photoelectron spectroscopy (XPS) and near edge X-ray absorption fine structure (NEXAFS)spectroscopy.
Autor*innen: | Paul M. Dietrich, Nele Lange, Andreas Lippitz, Markus Holzweber, N. Kulak, Wolfgang UngerORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Applied Surface Science |
Jahr der Erstveröffentlichung: | 2019 |
Organisationseinheit der BAM: | 6 Materialchemie |
6 Materialchemie / 6.1 Oberflächen- und Dünnschichtanalyse | |
Verlag: | Elsevier B.V. |
Jahrgang/Band: | 481 |
Erste Seite: | 10 |
Letzte Seite: | 15 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Biosensors; Click chemistry; H-terminated Si3N4 films; Near edge X-ray absorption fine structure; Streptavidin binding; X-ray photoelectron spectroscopy |
Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
DOI: | 10.1016/j.apsusc.2019.03.002 |
ISSN: | 0169-4332 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 06.08.2019 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 06.08.2019 |