Bio- and immuno-imaging by use of laser ablation ICP-MS
- Imuno-histochemical staining (IHC) of cancer biomarker on tissue sections is one of the most important analytical techniques for cancer diagnosis although standardization and quality management is tedious and differ significantly from clinic to clinic. Combining established IHC staining strategies with modern quantitative methods would increase it`s potential. We used element mass spectrometry (ICP-MS) and a new ink-jet printed internal standardization approach in combination with IHC staining. The printing strategy was utilized to improve elemental image resolution and reproducibility of paraffin embedded breast cancer tissue sections in laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) after conventional IHC staining as a model system to investigate the new capabilities of this technique.
Autor*innen: | Norbert JakubowskiORCiD |
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Koautor*innen: | Larissa Müller, Heike Traub, Diego Esteban-Fernández, Ulrich PanneORCiD, Antje Herrmann, E. Schellenberger, Janina KneippORCiD |
Dokumenttyp: | Vortrag |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2016 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Bio-imaging; Immuno-assays; Laser ablation |
Veranstaltung: | Ringvorlesung Analytik, Humboldt Universität zu Berlin |
Veranstaltungsort: | Berlin, Germany |
Beginndatum der Veranstaltung: | 10.06.2016 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 15.06.2016 |
Referierte Publikation: | Nein |
Eingeladener Vortrag: | Nein |