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Collision Specific Fragmentation of Oligonucleotides in Tandem Mass Spectrometry

  • Tandem MS experiments allow the fast acquisition of spectral datasets with enormous size and unprecedented content of information. The most commonly used method is undoubtedly collision induced dissociation (CID). The relatively young technical adaptation of CID to modern day Orbitrap experiments needs a higher confinement voltage and has accordingly been named higher-energy collisional dissociation HCD. Despite the name the absolute value of transferred energy per collision in HCD is lower than in CID. Since for many proteins CID and HCD result in comparable fragmentation patterns the two techniques are often treated as interchangeable. This approach is, however, not without pitfalls as other classes of biomolecules often exhibit strongly pronounced fragmentation specificity. As an example, MS/MS experiments on several 4-mer oligonucleotides were conducted employing both collision methods and the results were thoroughly compared. It is apparent that not only the total fragment yieldTandem MS experiments allow the fast acquisition of spectral datasets with enormous size and unprecedented content of information. The most commonly used method is undoubtedly collision induced dissociation (CID). The relatively young technical adaptation of CID to modern day Orbitrap experiments needs a higher confinement voltage and has accordingly been named higher-energy collisional dissociation HCD. Despite the name the absolute value of transferred energy per collision in HCD is lower than in CID. Since for many proteins CID and HCD result in comparable fragmentation patterns the two techniques are often treated as interchangeable. This approach is, however, not without pitfalls as other classes of biomolecules often exhibit strongly pronounced fragmentation specificity. As an example, MS/MS experiments on several 4-mer oligonucleotides were conducted employing both collision methods and the results were thoroughly compared. It is apparent that not only the total fragment yield but also the identity of the observed fragments differ significantly between the two methods.zeige mehrzeige weniger

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Metadaten
Autor*innen:Stefanie Ickert
Koautor*innen:Jens Riedel, S Beck, M.W. Linscheid
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2017
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:DNA; Mass Spectrometry; Tandem MS
Veranstaltung:Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie DGMS
Veranstaltungsort:Kiel, Germany
Beginndatum der Veranstaltung:05.03.2017
Enddatum der Veranstaltung:05.03.2017
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:05.10.2017
Referierte Publikation:Nein
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