Shape engineered TiO2 nanoparticles in Caenorhabditis elegans: a Raman imaging based approach to assist tissue-specific toxicological studies
- In vivo detailed spatial distribution of TiO2 NPs was here investigated for the first time using a 2D chemical imaging analysis based on Confocal Raman Spectroscopy. The invertebrate nematode C. elegans was employed as prototypical model of living organism. Rod, bipyramidal and quasi-spherical engineered TiO2 NPs with different primary particle sizes and agglomeration states were prepared, characterized and then administered to nematodes. Exploiting the typical fingerprint of TiO2 in the Raman spectrum, we monitored the biodistribution of NPs inside the worm using a non-invasive, label-free method. The high spatial resolution chemical imaging and the specificity of the Raman technique in the localization of TiO2 NPs helped in the design of behavioral C. elegans studies aimed at elucidating the relationship among the size, shape, and agglomeration state of NPs and their ability to induce specific toxic effects.
Autor*innen: | L. Iannarelli |
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Koautor*innen: | A. M. Giovanozzi, L. Diomede, F. Morelli, F. Viscotti, V. Maurino, P. Bigini, G. Martra, G. Spoto, Erik Ortel, Vasile-Dan Hodoroaba, A. M. Rossi |
Dokumenttyp: | Vortrag |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2016 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Nanoparticles; Raman imaging; TiO2; Toxicological studies |
Veranstaltung: | 16th European Conference on Applications of Surface and Interface Analysis ECASIA'15 |
Veranstaltungsort: | Fortaleza, Brazil |
Beginndatum der Veranstaltung: | 14.08.2016 |
Enddatum der Veranstaltung: | 19.08.2016 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 14.11.2016 |
Referierte Publikation: | Nein |
Eingeladener Vortrag: | Nein |