Photophysics and release kinetics of enzyme-activatable optical probes based on H-dimerized fluorophores on self-immolative linkers
- A series of activatable optical probes for the model enzyme penicillin G amidase based on intramolecular formation of non-fluorescent H-dimer between two identical dyes were synthesized. The probes are based on a self-immolative linker, which allows positioning both dyes in close spatial proximity to ensure efficient quenching of probes with absorption and fluorescence emission in the near-infrared (NIR) range. A detailed photophysical investigation of the novel optical probes led to a revision of a previously anticipated quenching mechanism and revealed their potential for optimizing the performance of activatable probes based on H-dimer formation. A kinetic analysis indicated that the fluorescence progress curves can be used to qualitatively extract enzyme kinetic parameters.
Autor*innen: | Jagoda Sloniec, Ute Resch-GengerORCiD, Andreas Hennig |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | The journal of physical chemistry / B |
Jahr der Erstveröffentlichung: | 2013 |
Herausgeber (Institution): | American Chemical Society |
Verlag: | Soc. |
Verlagsort: | Washington, DC |
Jahrgang/Band: | 117 |
Ausgabe/Heft: | 46 |
Erste Seite: | 14336 |
Letzte Seite: | 14344 |
DOI: | 10.1021/jp409388b |
ISSN: | 1520-6106 |
ISSN: | 1520-5207 |
ISSN: | 1089-5647 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 20.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 27.01.2014 |