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Shape engineered TiO2 nanoparticles in Caenorhabditis elegans: a Raman imaging based approach to assist tissue-specific toxicological studies

  • Titanium dioxide (TiO₂) nanoparticles (NPs) are one of the main sources of the nanoparticulate matter to which humans are directly exposed and several studies have demonstrated their potential toxic effects. The in vivo detailed spatial distribution of TiO₂ NPs is investigated herein for the first time, using a 2D chemical imaging analysis based on confocal Raman spectroscopy. The invertebrate nematode C. elegans was employed as a prototypical model of living organisms. Rod, bipyramidal and quasispherical engineered TiO₂ NPs with different primary particle sizes and agglomeration states were prepared, characterized and then administered to nematodes. Exploiting the typical fingerprint of TiO₂ in the Raman spectrum, we monitored the biodistribution of NPs inside the worm using a non-invasive, label-free method. The high spatial resolution chemical imaging and the specificity of the Raman technique in the localization of TiO₂ NPs helped in the design of behavioral C. elegans studiesTitanium dioxide (TiO₂) nanoparticles (NPs) are one of the main sources of the nanoparticulate matter to which humans are directly exposed and several studies have demonstrated their potential toxic effects. The in vivo detailed spatial distribution of TiO₂ NPs is investigated herein for the first time, using a 2D chemical imaging analysis based on confocal Raman spectroscopy. The invertebrate nematode C. elegans was employed as a prototypical model of living organisms. Rod, bipyramidal and quasispherical engineered TiO₂ NPs with different primary particle sizes and agglomeration states were prepared, characterized and then administered to nematodes. Exploiting the typical fingerprint of TiO₂ in the Raman spectrum, we monitored the biodistribution of NPs inside the worm using a non-invasive, label-free method. The high spatial resolution chemical imaging and the specificity of the Raman technique in the localization of TiO₂ NPs helped in the design of behavioral C. elegans studies aimed at elucidating the relationship among the size, shape, and agglomeration state of NPs and their ability to induce specific toxic effects. Rod-shaped NPs were the most toxic, greatly impairing pharyngeal function, reproduction and larval growth; this indicates that the rod shape, more than the bipyramidal and spherical shapes, enables NPs to interact with biological systems. These findings indicate that this Raman-nematode combined approach represents a step forward in the field of detection of NPs in living organisms, and being rapid and inexpensive enough, it can be applied as the first screening for the ability of NPs to biodistribute and exert toxicological properties in vivo.zeige mehrzeige weniger
Metadaten
Autoren/innen:L. Iannarelli, A. M. Giovannozzi, F. Morelli, F. Viscotti, P. Bigini, V. Maurino, G. Spotto, G. Martra, Erik Ortel, Vasile-Dan Hodoroaba, A. M. Rossi, L. Diomede
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):RSC Advances
Jahr der Erstveröffentlichung:2016
Organisationseinheit der BAM:6 Materialschutz und Oberflächentechnik
6 Materialschutz und Oberflächentechnik / 6.1 Oberflächenanalytik und Grenzflächenchemie
Veröffentlichende Institution:Bundesanstalt für Materialforschung und -prüfung (BAM)
Verlag:Royal Society of Chemistry
Verlagsort:Thomas Graham House, Cambridge
Jahrgang/Band:6
Erste Seite:70501
Letzte Seite:70509
Freie Schlagwörter:Caenorhabdtis elegans; Micro-Raman imaging; Nanoparticles; Nanotoxicology; Titanium dioxide
Themenfelder/Aktivitätsfelder der BAM:Analytical Sciences
Analytical Sciences / Oberflächen- und Grenzflächenanalytik
DOI:https://doi.org/10.1039/c6ra09686g
URN:urn:nbn:de:kobv:b43-369695
URL:http://pubs.rsc.org/en/content/articlelanding/2016/ra/c6ra09686g#!divAbstract
URL:http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord;UT=WOS:000381512800100
ISSN:2046-2069
Verfügbarkeit des Volltexts:Volltext-PDF für die Öffentlichkeit verfügbar ("Open Access")
Lizenz (Deutsch):License LogoCreative Commons - Namensnennung-Nicht kommerziell
Datum der Freischaltung:27.07.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:30.09.2016
Schriftenreihen ohne Nummerierung:Wissenschaftliche Artikel der BAM