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Need for and metrological approaches towards standardization of fluorescence measurements from the view of national metrology institutes

  • The need for standardization in fluorescence measurements to improve quality assurance and to meet regulatory demands is addressed from the viewpoint of National Metrology Institutes (NMIs). Classes of fluorescence standards are defined, including instrument calibration standards for the determination and correction of instrument bias, application-specific standards based on commonly used fluorescent labels, and instrument validation standards for periodic checks of instrument performance. The need for each class of standard is addressed and on-going efforts by NMIs and others are described. Several certified reference materials (CRMs) that have recently been developed by NMIs are highlighted. These include spectral correction standards, developed independently by both NIST and BAM (Germany), and fluorescence intensity standards for flow cytometry, developed by NIST. In addition, future activities at both institutes are addressed such as the development of day-to-day intensityThe need for standardization in fluorescence measurements to improve quality assurance and to meet regulatory demands is addressed from the viewpoint of National Metrology Institutes (NMIs). Classes of fluorescence standards are defined, including instrument calibration standards for the determination and correction of instrument bias, application-specific standards based on commonly used fluorescent labels, and instrument validation standards for periodic checks of instrument performance. The need for each class of standard is addressed and on-going efforts by NMIs and others are described. Several certified reference materials (CRMs) that have recently been developed by NMIs are highlighted. These include spectral correction standards, developed independently by both NIST and BAM (Germany), and fluorescence intensity standards for flow cytometry, developed by NIST. In addition, future activities at both institutes are addressed such as the development of day-to-day intensity standards.zeige mehrzeige weniger

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Metadaten
Autor*innen:P.C. DeRose, L. Wang, A.K. Gaigalas, G.W. Kramer, Ute Resch-GengerORCiD, Ulrich PanneORCiD
Persönliche Herausgeber*innen:Ute Resch-Genger, O.S. Wolfbeis
Dokumenttyp:Beitrag zu einem Sammelband
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Standardization and Quality Assurance in Fluorescence Measurements I - Techniques
Jahr der Erstveröffentlichung:2008
Verlag:Springer
Jahrgang/Band:5
Ausgabe/Heft:Part A
Erste Seite:33
Letzte Seite:62
Freie Schlagwörter:Calibration; Comparability; Emission standard; Fluorescence spectroscopy; Fluorescent glass; Quality assurance; Quantification; Radiometry; Spectral correction; Spectral fluorescence standard; Standard; Traceability
DOI:10.1007/4243_2008_049
ISSN:1617-1306
ISBN:978-3-540-75206-6
Bemerkung:
Serientitel: Springer Series on Fluorescence – Series title: Springer Series on Fluorescence
Verfügbarkeit des Dokuments:Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
Bibliotheksstandort:Sonderstandort: Publica-Schrank
Datum der Freischaltung:19.02.2016
Referierte Publikation:Nein
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