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Poly(ethylene oxide)-block-poly(glutamic acid) coated maghemite nanoparticles: in vitro characterization and in vivo behaviour

  • Positively charged superparamagnetic iron oxide (SPIO) particles of maghemite were prepared in aqueous solution and subsequently stabilized with poly(ethylene oxide)-block-poly(glutamic acid) (PEO–PGA) at a hydrodynamic diameter of 60 nm. Depending on the amount of PEO–PGA used, this is accompanied by a switching of their zeta potentials from positive to negative charge (-33 mV). As a prerequisite for in vivo testing, the PEO–PGA coated maghemite nanoparticles were evaluated to be colloidally stable in water and in physiological salt solution for longer than six months as well in various buffer systems under physiological pH and salt conditions (AFM, dynamic light scattering). We excluded toxic effects of the PEO–PGA coated maghemite nanoparticles. We demonstrated by in vivo MR-imaging and 111In measurements a biodistribution of the nanoparticles into the liver comparable to carboxydextran coated superparamagnetic iron oxide nanoparticles (Resovist®) as a reference nanoscaled MRIPositively charged superparamagnetic iron oxide (SPIO) particles of maghemite were prepared in aqueous solution and subsequently stabilized with poly(ethylene oxide)-block-poly(glutamic acid) (PEO–PGA) at a hydrodynamic diameter of 60 nm. Depending on the amount of PEO–PGA used, this is accompanied by a switching of their zeta potentials from positive to negative charge (-33 mV). As a prerequisite for in vivo testing, the PEO–PGA coated maghemite nanoparticles were evaluated to be colloidally stable in water and in physiological salt solution for longer than six months as well in various buffer systems under physiological pH and salt conditions (AFM, dynamic light scattering). We excluded toxic effects of the PEO–PGA coated maghemite nanoparticles. We demonstrated by in vivo MR-imaging and 111In measurements a biodistribution of the nanoparticles into the liver comparable to carboxydextran coated superparamagnetic iron oxide nanoparticles (Resovist®) as a reference nanoscaled MRI contrast medium. This was enforced by a detailed visualization of our nanoparticles by electron microscopy of liver tissue sections. Furthermore, our results indicate that 15% of the injected PEO–PGA coated maghemite nanoparticles circulate in the blood compartment for at least 60 min after i.v. application.zeige mehrzeige weniger

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Metadaten
Autor*innen:L. Kaufner, R. Cartier, R. Wüstneck, I. Fichtner, S. Pietschmann, H. Bruhn, Dagmar Schütt, Andreas ThünemannORCiD, U. Pison
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Nanotechnology
Jahr der Erstveröffentlichung:2007
Verlag:IOP Publishing Ltd.
Verlagsort:Bristol
Jahrgang/Band:18
Ausgabe/Heft:11
Erste Seite:115710 (1
Letzte Seite:10)
Freie Schlagwörter:Block-Copolymer; Nano Particle; Nanotechnology
DOI:10.1088/0957-4484/18/11/115710
ISSN:0957-4484
ISSN:1361-6528
Verfügbarkeit des Dokuments:Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
Bibliotheksstandort:Sonderstandort: Publica-Schrank
Datum der Freischaltung:19.02.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:21.01.2008
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