Quantification of the adhesion force of E. coli on Ti via single-cell force spectroscopy
- Antibiotic resistance is a growing global problem which poses a massive threat to human health. Although human activity contributes to the acceleration of the process, bacteria have a self-driven stabilisation mechanism to protect themselves from such and other external threats: biofilm formation. Nonetheless, it is the adhesion of a single bacterial cell to a surface that triggers the formation of such network of biomolecules and microorganisms, as well as its hazardous consequences. The main objective of this work was to quantify the adhesion force of a single E. coli cell on a Ti substrate via the AFM-related single-cell force spectroscopy, with both the cell and the substrate material being of high clinical relevance. A set of 25 x 25 force displacement curves was acquired with a maximum force of 3.2 nN without dwell time, yielding a topography map and an adhesion force map that showed to be correlated. A mean adhesion force of 0.85 ± 0.175 nN was measured and the presence of cellAntibiotic resistance is a growing global problem which poses a massive threat to human health. Although human activity contributes to the acceleration of the process, bacteria have a self-driven stabilisation mechanism to protect themselves from such and other external threats: biofilm formation. Nonetheless, it is the adhesion of a single bacterial cell to a surface that triggers the formation of such network of biomolecules and microorganisms, as well as its hazardous consequences. The main objective of this work was to quantify the adhesion force of a single E. coli cell on a Ti substrate via the AFM-related single-cell force spectroscopy, with both the cell and the substrate material being of high clinical relevance. A set of 25 x 25 force displacement curves was acquired with a maximum force of 3.2 nN without dwell time, yielding a topography map and an adhesion force map that showed to be correlated. A mean adhesion force of 0.85 ± 0.175 nN was measured and the presence of cell appendages on the bacterial cell wall was verified through individual force-displacement curves. Bacterial viability was assessed after the measurements via live/dead staining.…
Autor*innen: | Judith Zubia Aranburu, Brunero Cappella, A. Zabala EgurenORCiD, L. Buruaga LamarainORCiD, A. Aginagalde LopezORCiD, Jörn BonseORCiD, Karin Schwibbert |
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Dokumenttyp: | Beitrag zu einem Tagungsband |
Veröffentlichungsform: | Graue Literatur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Spanisch): | Libro de Actas del XL Congreso Anual de la Sociedad Española de Ingeniería Biomédica |
Jahr der Erstveröffentlichung: | 2022 |
Organisationseinheit der BAM: | 4 Material und Umwelt |
4 Material und Umwelt / 4.1 Biologische Materialschädigung und Referenzorganismen | |
6 Materialchemie | |
6 Materialchemie / 6.2 Material- und Oberflächentechnologien | |
9 Komponentensicherheit | |
9 Komponentensicherheit / 9.5 Tribologie und Verschleißschutz | |
Herausgeber (Institution): | Sociedad Española de Ingeniería Biomédica |
Erste Seite: | 217 |
Letzte Seite: | 220 |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik | |
Freie Schlagwörter: | Atomic force microscopy; Bacteria; Force distance curve |
Themenfelder/Aktivitätsfelder der BAM: | Material |
Material / Nano | |
Umwelt | |
Umwelt / Umwelt-Material-Interaktionen | |
Veranstaltung: | XL Congreso Anual de la Sociedad Española de Ingeniería Biomédica CASEIB 2022 |
Veranstaltungsort: | Valladolid, Spain |
Beginndatum der Veranstaltung: | 23.11.2022 |
Enddatum der Veranstaltung: | 25.11.2022 |
ISBN: | 978-84-09-45972-8 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 27.02.2023 |
Referierte Publikation: | Nein |