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Surface functionality independent conjugation of biomolecules to colloidal metal-organic frameworks and their exemplified application in immuno-assays

  • Metal-organic framework (MOF) colloids hold great potential for bioanalytical and biomedical applications due to their unique features. These include responsive luminescent properties and exceptionally high loading capacities for small molecular drugs. However, currently the lack of a surface functionality independent method for biomolecule conjugation is strongly limiting the advancement of colloidal MOFs in bioanalytical or biomedical applications. Bioanalytical methods, especially for environmental analysis, would benefit dramatically when responsive luminescent properties of MOFs could be coupled with a specific antibody interaction. Targeted drug delivery in biomedical applications often requires specificity towards tissues of interest in addition to a high drug loading capacity. Thus, both envisioned applications require biomolecules and in particular antibodies to be conjugated to colloidal MOFs. Here, we propose a robust and easy to handle method that is suitable for a wideMetal-organic framework (MOF) colloids hold great potential for bioanalytical and biomedical applications due to their unique features. These include responsive luminescent properties and exceptionally high loading capacities for small molecular drugs. However, currently the lack of a surface functionality independent method for biomolecule conjugation is strongly limiting the advancement of colloidal MOFs in bioanalytical or biomedical applications. Bioanalytical methods, especially for environmental analysis, would benefit dramatically when responsive luminescent properties of MOFs could be coupled with a specific antibody interaction. Targeted drug delivery in biomedical applications often requires specificity towards tissues of interest in addition to a high drug loading capacity. Thus, both envisioned applications require biomolecules and in particular antibodies to be conjugated to colloidal MOFs. Here, we propose a robust and easy to handle method that is suitable for a wide range of MOF templates and that allows reliable conjugation of biomolecules. Colloidal ZIF-8 MOFs with particle size between 300nm and 5µm were used as templates to adsorb polymeric multilayer through a Layer-by-Layer self-assembly process. Multilayer build up was confirmed by the change in zeta-potential of particles upon polyelectrolyte adsorption as well as dynamic light scattering experiments and SEM/TEM microscopy. Subsequent biomolecule conjugation to functional groups of the polymeric multilayer on the surface of colloidal MOFs was achieved by established carbodiimide/succinimide conjugation chemistry. Successful and stable conjugation was confirmed by employing fluorescent labeled biomolecules. Subsequently, colloidal MOF-antibody (IgG) conjugates were utilized in a solid-phase immuno assay using antibodies against carbamazepine and lithocholic acid, two interesting markers in environmental analysis. In brief, mouse anti-CBZ (Carbamazepine) and rabbit anti-lithocholic acid were separately and covalently immobilized on 2D polystyrene surfaces to form a small micro spot array. Two separate populations of colloidal MOF particles were conjugated with goat anti mouse IgG and goat anti rabbit IgG, respectively. The different fluorescent labels of the two MOF colloid populations allowed the determination of binding selectivity and strength of the colloidal MOF-antibody conjugates to the immobilized binding partners. The ability to coat MOFs in solution independent of their surface charge with polymers and subsequently to conjugate biomolecules to their surface makes this method a powerful tool to foster bioanalytical, biomedical as well as other applications.zeige mehrzeige weniger

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  • MOF-2016-Long_Beach_California_S_Beyer_BAM_13_and_1_8.pdf
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Metadaten
Autor*innen:Sebastian Beyer
Koautor*innen:Franziska Emmerling, Rudolf Schneider
Dokumenttyp:Vortrag
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2016
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Immunoassay
Veranstaltung:MOF-2016 Conference Long Beach, California
Veranstaltungsort:Long Beach, California, USA
Beginndatum der Veranstaltung:11.09.2016
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:30.11.2016
Referierte Publikation:Nein
Eingeladener Vortrag:Nein
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