• Treffer 5 von 10
Zurück zur Trefferliste

Investigation of cell culture volatilomes using solid phase micro extraction: options and pitfalls exemplified with adenocarcinoma cell lines

  • Three strategies to sample volatile organic compounds (VOC) from lung cancer cell lines cultured in vitro were compared. Headspace solid phase microextraction was applied in situ to culture flasks and alternatively to subsamples of headspace gas or to nutrient solution subsamples followed by gas chromatography–mass spectrometry. The direct quantification of 55 VOC in the headspace of cell cultures was validated and is discussed with respect to reproducibility and system-related interferences. The role of the VOC background from culture media and usually employed polystyrene culture vessels is examined and was seen to invoke potentially misleading conclusions. The commercial A549 and two further adenocarcinoma cell lines displayed largely similar VOC profiles with distinct differences regarding certain individual substances. There is evidence for the inappropriateness of the standard cell culturing methods in the search for volatile cancer markers.

Volltext Dateien herunterladen

  • Schallschmidt_Investigation of cell culture volatilomes.pdf

Metadaten exportieren

Weitere Dienste

Teilen auf Twitter Suche bei Google Scholar
Autoren/innen:Kristin Schallschmidt, Roland Becker, Christian Jung, J. Rolff, I. Fichtner
Titel des übergeordneten Werkes (Englisch):Journal of chromatography B
Jahr der Erstveröffentlichung:2015
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.7 Organische Spuren- und Lebensmittelanalytik
Verlag:Elsevier B.V.
Verlagsort:Amsterdam [u.a.]
Erste Seite:158
Letzte Seite:166
Freie Schlagwörter:A549; Alcohol marker; Fortification; GC–MS; HPLC-MS/MS; Long term stability; Lung cancer cell culture; Micropulverization; SPME; VOC; Volatilome
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:20.02.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:30.11.2015