Determination of chromatographic parameters of a miniaturized AF4 channel and its comparison with a conventioanl AF4 channel
- The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP) using Asymmetrical Flow Field-Flow Fractionation (AF4) was investigated and compared with a conventional AF4 system. To develop standard separation methods, experimental parameters like cross flow, gradient profile and injection time were varied and optimized. Corresponding chromatographic parameters were calculated and compared. Our results indicate that the chromatographic resolution in the miniaturized channel is lower, whereas significantly shorter analyses time and less solvent consumption were obtained. Moreover, the limit of detection (LOD) and limit of quantification (LOQ) obtained from hyphenation with a UV-detector are obviously lower than in a conventional channel, which makes the miniaturized channel interesting for trace analysis.
Autor*innen: | Zengchao You |
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Koautor*innen: | Steffen WeidnerORCiD |
Dokumenttyp: | Vortrag |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2016 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Nanoparticles separation asymetrical flow field flow fractionation |
Veranstaltung: | BAM Seminar |
Veranstaltungsort: | Berlin, Germany |
Beginndatum der Veranstaltung: | 31.08.2016 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 15.01.2019 |
Referierte Publikation: | Nein |
Eingeladener Vortrag: | Nein |