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Needs and Tools Standardization of Fluorescence

  • Photoluminescence techniques are amongst the most widely used tools in the life sciences, with new and exciting applications in medical diagnostics and molecular imaging continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. General drawbacks are, however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are also time-dependent due to aging of instrument-components, and difficulties to measure absolute fluorescence intensities. Moreover, scattering samples require special measurement geometries and emerging optical Reporters with emission > 1000 nm strategies for reliable measurements in the second diagnostic window or short wavelength infrared (SWIR) for the comparison of material performance and the rational design of new fluorophores with improved properties. Here, we provide a briefPhotoluminescence techniques are amongst the most widely used tools in the life sciences, with new and exciting applications in medical diagnostics and molecular imaging continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. General drawbacks are, however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are also time-dependent due to aging of instrument-components, and difficulties to measure absolute fluorescence intensities. Moreover, scattering samples require special measurement geometries and emerging optical Reporters with emission > 1000 nm strategies for reliable measurements in the second diagnostic window or short wavelength infrared (SWIR) for the comparison of material performance and the rational design of new fluorophores with improved properties. Here, we provide a brief overview over different types of fluorescence standards for instrument calibration and performance control. Also, strategies to versatile method-adaptable liquid and solid fluorescence standards for different fluorescence parameters and applications are presented. This includes spectral emission standards for the traceable determination of the wavelengthdependent spectral responsivity (emission correction curve) of fluorescence measuring devices in the UV/vis/NIR, solid multi-emitter systems for daily instrument performance control, and new standards for the relative determination of the fluorescence quantum yield, the key performance parameter of all emitters. The latter standards enable also performance Control of increasingly used integrating sphere setups.zeige mehrzeige weniger

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Metadaten
Autor*innen:Ute Resch-GengerORCiD
Koautor*innen:Katrin Hoffmann, Jutta Pauli, Arne Güttler, Christian Würth, Maria Richter, Thomas Schneider
Dokumenttyp:Vortrag
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2018
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.2 Biophotonik
Freie Schlagwörter:Calibration slide; Dye; Emission standard; Fluorescence; Glass; Instrument performance validation; Quality assurance; Quantum yield; Reference material; Standardization
Themenfelder/Aktivitätsfelder der BAM:Chemie und Prozesstechnik
Veranstaltung:15th International Symposium on Biological and Environmental Reference Materials
Veranstaltungsort:Berlin, Germany
Beginndatum der Veranstaltung:23.09.2018
Enddatum der Veranstaltung:26.09.2018
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:29.10.2018
Referierte Publikation:Nein
Eingeladener Vortrag:Nein
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