Zitieren Sie bitte immer diese URN: urn:nbn:de:0183-lab0000160

Survey results on nucleic acid tests of infectious diseases: present status and need for rapid and patient near diagnostics

  • This survey will discuss current and emerging isothermal and rapid polymerase chain reaction (PCR) based nucleic acid amplification methods for patient near diagnostics. To assess the clinical need of rapid diagnostics for infectious diseases based on nucleic acid tests (NATs) we performed and analysed a questionnaire among laboratories participating in corresponding INSTAND ring trials for external quality assurance. The questions concerning new amplification technologies like isothermal nucleic acid amplification, potentially suited to significantly decrease turnaround times, were complemented by questions to evaluate the present status of NATs. Besides end-users, companies were also addressed by sending out a manufacturer specific questionnaire. Analysis of the answers from 48 laboratories in 14 European countries revealed that a much shorter turnaround time is requested for selected pathogens compared to about 2 h or longer when applying temperature cycling amplification, i.e. PCR.This survey will discuss current and emerging isothermal and rapid polymerase chain reaction (PCR) based nucleic acid amplification methods for patient near diagnostics. To assess the clinical need of rapid diagnostics for infectious diseases based on nucleic acid tests (NATs) we performed and analysed a questionnaire among laboratories participating in corresponding INSTAND ring trials for external quality assurance. The questions concerning new amplification technologies like isothermal nucleic acid amplification, potentially suited to significantly decrease turnaround times, were complemented by questions to evaluate the present status of NATs. Besides end-users, companies were also addressed by sending out a manufacturer specific questionnaire. Analysis of the answers from 48 laboratories in 14 European countries revealed that a much shorter turnaround time is requested for selected pathogens compared to about 2 h or longer when applying temperature cycling amplification, i.e. PCR. In this context, most frequently mentioned were MRSA, norovirus, influenza A and B viruses, cytomegalovirus (CMV) as well as hepatitis B virus (HBV) and hepatitis C virus (HCV). At present, 8% of the laboratories having participated in this survey apply isothermal amplification of nucleic acid to identify infectious pathogens.zeige mehrzeige weniger
Metadaten
Autoren/innen:J. Neukammer, M. Hussels, A. Kummrow, A. Devonshire, C. Foy, J. Huggett, H. Parkes, J. Zel, M. Milavec, H. Schimmel, Wolfgang UngerORCiD, M. Akgoz, V. McHugh, H.-P. Grunert, H. Zeichhardt
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Deutsch):GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien
Jahr der Erstveröffentlichung:2014
Veröffentlichende Institution:Bundesanstalt für Materialforschung und -prüfung (BAM)
Verlag:GMS
Verlagsort:Düsseldorf
Jahrgang/Band:6
Erste Seite:1
Letzte Seite:11
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Bacteria detection; Infectious diseases; Isothermal nucleic acid amplification; NAT; Nucleic acid tests; PCR; Questionnaire; Status report; Virus detection
DOI:https://doi.org/10.3205/lab000016
URN:urn:nbn:de:0183-lab0000160
URL:http://www.egms.de/static/en/journals/lab/2015-6/lab000016.shtml
ISSN:1869-4241
Verfügbarkeit des Dokuments:Datei für die Öffentlichkeit verfügbar ("Open Access")
Lizenz (Deutsch):License LogoCreative Commons - Namensnennung
Datum der Freischaltung:23.05.2016
Referierte Publikation:Nein
Schriftenreihen ohne Nummerierung:Wissenschaftliche Artikel der BAM