TY - CONF A1 - von Werder, Julia A1 - Stohl, Leonie T1 - Biorezeptive Betonfassaden: Algenbiofilme als Gestaltungselement und zur Verbesserung des Stadtklimas N2 - Der Vortrag fasst den aktuellen Stand der Forschung an der BAM zum Thema "Biorezeptive Betonfassaden" zusammen. Während im Projekt Funktionsbeton der Einfluss der Textur von ultrahochfestem Beton auf die Biorezeptivität im Vordergrund steht, wird im Projekt Algenbiofilmfassade untersucht, inwieweit ein Biofilm künstlich auf Betonsubstraten etabliert werden kann. T2 - BUGG-Tag der Forschung und Lehre Gebäudegrün 2022 CY - Stuttgart, Germany DA - 10.05.2022 KW - Biofilm KW - Biorezeptivität KW - Beton KW - UHPC KW - Fassade PY - 2022 AN - OPUS4-56388 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Stohl, Leonie A1 - von Werder, Julia T1 - Bioreceptivity of Concrete Facades N2 - In recent years, the demand for new and sustainable products has been constantly rising. Especially cementitious materials are known for their high carbon footprint and novel approaches will be needed to counterbalance that. The BAM departments 7.1 (building materials) and 4.0 (Material and the environment) try to improve the sustainability of concrete panels by establishing a biofilm on the weather protection layer. Using an ultra-high-performance-concrete allows the production of very thin facade tiles with a small carbon footprint. Furthermore, the durability of these tiles is expected to surpass the one of “normal” concrete facades and offer the possibility of modular renewing of defective tiles instead of a wall, or even remodeling a whole building. The controlled application of biofilms on facades aims to improve biodiversity, microclimate, and air quality in cities. Furthermore, the greening of typically gray surfaces could improve the mental health of the residents. The project focuses on testing a multiplicity of concrete samples, varying e.g., in roughness or pH. The bioreceptivity is analyzed via laboratory tests, as well as in perennial outdoor experiments. T2 - Biology versus building materials: from biocides to bio-receptivity CY - Online meeting DA - 08.02.2022 KW - Bioreceptivity KW - Algea KW - Concrete KW - Biofilm PY - 2022 AN - OPUS4-54470 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gorbushina, Anna T1 - A new tool in material science - Targeted disruption of melanin synthesis in rock-inhabiting fungi N2 - The ability to survive almost absolute dehydration through air-drying is a remarkable feature of rock-inhabiting microcolonial fungi (MCF), which colonise rock surfaces in hot and cold deserts. Understanding of the underlying mechanisms which allow this group of fungi to conquer natural and man-made environments requires a set of modern biological techniques and approaches that are under development in our laboratory. We will present an overview of the targeted disruption of melanin biosynthesis genes in the rock-inhabiting Knufia petricola and give inside into the lines of research and the network of supporting laboratories that made this progress possible. T2 - 6. Meeting of the ISHAM Working Group "Black Yeasts and Relatives" CY - Viterbo, Italy DA - 15.09.2016 KW - Black fungi KW - SAB KW - Biofilm PY - 2016 AN - OPUS4-37693 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gorbushina, Anna T1 - Microbiology / biofilms in material research and testing N2 - In the modern world there is an increased understanding that design and performance monitoring of materials have to be tested in connection to chemical, physical and (micro)biological challenges. A systematic study on how biofilms interact with materials and what could be done to engineer biofilms and/or materials in order to maximize the resistance of the material (surface) or the resistance the biofilm-modified material (bulk) is in strong need. In the Department “Materials and the Environment” of the BAM new experimental platform is being developed. With the help of different type of device for high throughput and microbiologically-controlled environment simulation we establish a new approach to clarify the mechanisms of biofilm/material interactions. Despite the focus on fundamental research, the main results of this project proposal will be transferable into material technology and construction chemistry and will influence the development of standardization in this topic. As the interactions of biofilms and materials have implications for most constructions as well as climate change, the results of the research generates additional value. T2 - Initialgespräch - DFG-Forschungsgruppe "Mikrobiologie/Biofilme" CY - Karlsruhe, Germany DA - 14.11.2019 KW - Biofilm KW - Microbiology KW - Black fungi KW - Solar panel PY - 2019 AN - OPUS4-50199 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gorbushina, Anna T1 - Life on the rocks and other hard surfaces N2 - The story of how black fungi survive harsh conditions on sun-exposed desert rocks and material surfaces is fascinating. In the presentation examples of how knowledge of these organisms can be of practical value (e.g., in biodeterioration studies, such as on stone markers in cemeteries) would be given. Among other stories, the example of how roof tiles amended with a titanium oxide layers as a biocide actually selected for the black fungi will be told. A hypothesis about how these organisms would likely be found on solar panels and some early BAM work in that area will be presented. This then brings the connection to a study ripe for investigation in North Carolina. The talk presents the development of a study aiming at deciphering the influence of microbial biofilm formation on the energy conversion efficiency of solar photovoltaic panels or modules at two facilities (one facility under the impact of high intensity of animal agriculture and high deposition of ammonia from atmosphere and the other with low animal agriculture and lower atmospheric ammonia deposition) in North Carolina. The main hypothesis of the study is that microbial biofilm formation on solar photovoltaic panels will lead to significant decreases in energy conversion efficiency of solar photovoltaic modules and biofilm formation will also be accelerated by high ammonia concentration in the ambient atmosphere and high nitrogen deposition. T2 - Dr. Michael D. Aitken Symposium CY - Chapel Hill, NC, USA DA - 08.11.2019 KW - Black fungi KW - Solar panel KW - Biofilm PY - 2019 AN - OPUS4-50125 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gorbushina, Anna A1 - Breitenbach, Romy A1 - Gerrits, Ruben A1 - Knabe, Nicole A1 - Voigt, Oliver T1 - Rock biofilms in nature and in the lab N2 - Essential processes necessary for the establishment and maintenance of rock biofilms include photosynthesis, production of extracellular polymeric substances, substrate penetration and nutrient enrichment from the atmosphere. This natural functional diversity is supported by a complex biofilm community consisting of heterotrophic and phototrophic microorganisms. While heterotrophic rock biofilm-formers are dominated by stress-tolerant microcolonial ascomycetes, phototrophs might be represented by diverse algae and cyanobacteria. Reduction of model systems to the genetically tractable minimum, a proven successful experimental strategy in different symbiotic systems, is now applied to rock biofilms. These complex and important natural systems are simulated in the lab using genetic methods coupled with continuous microscopic and analytical (microscopic as well as geochemical and biochemical) observations. Our in vitro bipartite model includes free-living and symbiosis-competent, genetically tractable microorganisms – a rock-inhabiting fungus Knufia petricola A95 and a cyanobacterium Nostoc punctiforme strain ATCC 29133. To accurately reflect the development of a rock biofilm, contacts of these two genetically tractable partners are studied under well-controlled laboratory conditions. This experimental strategy is strongly supported by the knockout mutants of Knufia petricola which have been recently created and will be compared in their action on mineral surfaces. An impact of Knufia petricola strain A95 with or without protective pigments on mineral adhesion and alteration will be presented. T2 - XXX Congress of the Italian Lichen Society CY - Turin, Italy DA - 13.09.2017 KW - Knufia petricola KW - Biofilm KW - Rock-inhabiting fungus PY - 2017 AN - OPUS4-43136 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Stephan, Ina A1 - Knabe, Nicole A1 - Koerdt, Andrea A1 - Martin-Sanchez, Pedro Maria A1 - Schwibbert, Karin A1 - Özcan Sandikcioglu, Özlem A1 - Kunte, Hans-Jörg A1 - Schreiber, Frank A1 - Pietsch, Franziska A1 - McMahon, Dino Peter A1 - Stephan, Ina A1 - Villa, F. A1 - Capitelli, F. A1 - Sand, W. T1 - Reference Organisms in Materials Science: Why and How? N2 - Materials are subject to environmental constraints that include biological, chemical and physical factors. To gain confidence about durability and long-term performance of any material, environmental resistance testing procedures have to be amended with modern simulation procedures that include biological components. In fact, any environmentally exposed surface at temperatures lower than 121 °C will be home to microbial growth, even at high salt concentrations, extreme pH, environmental pollution, low water potential, and intense irradiation – everywhere where water is liquid and available. As a consequence, complex microbial ecosystems called biofilms are self-sufficient and found on almost all solid-air-water interfaces. Obviously, environmental changes perturb biofilm development but over a number of seasons, these changes result in relatively stable microbial communities peculiar and adapted to a particular niche and material. Certain microbial settlers are indicative of, and in a real sense mark, a particular biofilm and can, thus, be considered as “reference organisms”. Characteristic reference organisms’ peculiar to specific material-inhabiting communities can be isolated, identified, characterised and used in standard test procedures as well as research into materials science (materials improvement). In this presentation classical microbiological, genetic and molecular methods for studying reference organisms and their roles in materials deterioration will be presented. We will present a set of different reference organisms that are currently in focus of our research and testing development. T2 - IBBS 17 - The 17th International Biodeterioration & Biodegradation Symposium CY - Manchester, United Kingdom DA - 06.09.2017 KW - Biofilm KW - Biodeterioration KW - Knufia petricola KW - Bioprospecting fuels KW - Solar panels PY - 2017 AN - OPUS4-42460 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gorbushina, Anna A1 - Villa, F. A1 - Capitelli, F. T1 - Studying colonization of stone surfaces – what can we learn from model biofilms growing in flow-through chambers? N2 - Soil formation on weathering rock surfaces is intrinsically connected with the development of primary microbial colonization at the atmosphere-lithosphere interface. A great number and variety of microorganisms is involved in these microbial communities, which are dominated by fungi, algae, cyanobacteria and heterotrophic bacteria. Rock-inhabiting life is ubiquitous on rock surfaces all around the world, but the laws of its establishment, and more important, quantification of its biodeterioration and geological input are possible only in well-controlled and simplified laboratory models. Here we would like to compare two model rock biofilm consisting of the heterotrophic and the phototrophic interacting partners. In the present work the growth of these model biofilms on diverse materials with different physical and chemical properties was investigated under well-controlled laboratory conditions. To clarify the role of environmental factors, the parameters temperature, light intensity and relative humidity were varied in growth test series. For an accelerated substrate colonization and to increase the biomass yield different flow-through chambers systems with semi-continuous cultures have been applied, simulating weathering conditions like flooding, desiccation and nutrient input. The biofilm development was studied by (i) confocal laser scanning and electron microscopy and (ii) qualitatively and quantitatively with respect to cell forms and biomass. A correlation between the presence of the model biofilm and mineral surface alteration as well as geochemical tracers of weathering were followed on various rock substrates (with differing geochemistry, porosity etc) exposed in another flow-through chamber, filled with crushed rock material. Under mentioned environmental conditions different types of flow-through chambers have been used and will be compared. T2 - Technoheritage CY - Cadiz, Spain DA - 21.05.2017 KW - Biofilm KW - Biodeterioration PY - 2017 AN - OPUS4-41136 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gorbushina, Anna A1 - Martin-Sanchez, Pedro Maria A1 - Gebhardt, Christopher A1 - Toepel, Jörg A1 - Barry, J. A1 - Munzke, N. A1 - Günster, Jens A1 - Broughton, William J. A1 - Porcar, M. T1 - Biofilms on solar panel surfaces – new materials, long-known colonisers N2 - Simple, microbial biofilms have prevailed since life began on Earth. Biofilms form at the interfaces of solids with gases or liquids and have multiple effects on Substrate and element cycles. In geobiological terms, the most interesting microbial communities are those that form on solids exposed to air (subaerial). Microbial colonisers of the atmosphere-lithosphere interface include algae, cyanobacteria, fungi as well as heterotrophic bacteria and they have colonised virtually every rock surface throughout the entire geological history of the Earth. In addition to sequestering carbon, sub-aerial biofilms (SABS) actively participate in rock weathering. Rock-inhabiting SABs are the primary settlers on lava following volcanic eruptions and on rocks following the retreat of glaciers. SABs especially dominate hostile environments in which growth of higher Vegetation is restricted especially in deserts, polar- and alpine regions. SABs are the primary colonisers of lithospheric (e.g. rocks) and anthropogenic Substrates (buildings, monuments, solar panels, etc.). Life at the solid material/atmosphere interface influences and is affected by both the underlying Substrate and the microclimate surrounding it. Although subaerial life is ubiquitous, how SABs develop and importantly degrade underlying Substrates can only be clarified in well-controlled experiments that often involve simplified model Systems, So far, biofilm development on solar panels has been studied using; (i) metagenomics; (ii) in situ microscopy; and (iii) classical microbiological methods that are both qualitative and quantitative. Here we suggest that solar panel biofilms are accessible and highly relevant objects to study microbial ecology, geobiology and biodeterioration. T2 - 7th Congress of European Microbiologists (FEMS 2017) CY - València, Spain DA - 09.07.2017 KW - SABs KW - Biodeterioration KW - Biofilm KW - Solar panel PY - 2017 AN - OPUS4-41189 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Stohl, Leonie A1 - von Werder, Julia T1 - Funktionalisierter Beton: Biorezeptivität von strukturiertem UHPC N2 - Understanding the fundamentals of bioreceptivity enables the developement of functionalized materials. Concrete as the most used building material worldwide is of special interest as microbially greened panels may represent an alternative to classic façade greening with plants. As standards are not ye established, material characterization in context as well as bioreceptivity assessment itself has been proven to be difficult. The presentation reviews first results of methods adapted for this application. T2 - Tagung des Instituts für Bioanalytik: Bewertung biozidhaltiger Baustoffe CY - Augsburg, Germany DA - 29.06.2023 KW - Biorezeptivität KW - Beton KW - UHPC KW - Biofilm PY - 2023 AN - OPUS4-58375 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -