TY - CONF A1 - Schneider, Rudolf T1 - Antikörperbasierte Sensorik für das Monitoring von Spurenstoffen im Wasser N2 - Der Vortrag beschreibt den Stand der Arbeiten an der BAM, Fachbereich Umweltanalytik zur Entwicklung von Sensoren für die Wasseranalytik. Er stellt die bisherigen Arbeiten da, die ausgehend von ELISAs, zunehmend portable Plattformen wie FPIA und LFIA umfassen. Beas-based Formate werden von mikrotiterplatten-basierten Techniken auf mikrofluidische Lab-on-Chip-Systeme übertragen, die bei geeigneter Miniaturisierung und Automatisierung als Sensorsysteme angesehen werden können. Der Vortrag endet mit einem Blick auf ein laufendes Monitoring-Projekt von anthropogenen Markern in Abwasser und charakterisiert die Treiber, die die Entwicklung von Sensoren für die Wasseranalytik in den letzten Jahren vorantreiben. T2 - Sitzung des Fachausschusses Wasser der Wasserchemischen Gesellschaft in der GDCh CY - Online meeting DA - 16.01.2024 KW - Biosensoren KW - Immunoassay KW - Antikörper PY - 2024 AN - OPUS4-59383 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf A1 - Hoffmann, Holger A1 - Panne, Ulrich T1 - LC-ELISA as a contribution to the validation of immunoassays N2 - Coupling ELISA and liquid chromatography yields a powerful too to detect cross-reacting substances, interfering matrix compounds and thus to validation of binding assays. T2 - CCQM Workshop: "Advances in Metrology in Chemistry and Biology" CY - Paris, France DA - 08.04.2019 KW - ELISA KW - Immunoassay KW - Validierung PY - 2019 AN - OPUS4-48322 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - An Electrochemical Immunosensor for Pharmaceutical Contaminants in Water N2 - Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast & cost-effective on-site analyses. Magnetic bead-based immunoassays allow for implementation into an immunosensor for online testing. Particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically (chronoamperometry) on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes. T2 - SALSA Make & Measure CY - Online meeting DA - 16.09.2021 KW - Immunosensor KW - Magnetic Beads KW - Amoxicillin KW - Diclofenac KW - Microfluidics KW - Chronoamperometry PY - 2021 AN - OPUS4-53307 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Raysyan, Anna A1 - Schneider, Rudolf T1 - Lateral Flow ImmunoAssays for the detection of bisphenol A emissions from plastic materials N2 - Bisphenol A (BPA) is a chemical that has been used in the production of plastics for more than 40 years. BPA released from bottles made of polycarbonates has been identified as a potential endocrine disrupting substance. Comprehensive research has been undertaken to test and verify its effect on animals and human beings. Many methods have been proposed for the determination of BPA, in particular, gas and liquid chromatography. The main disadvantages of such approaches are the high cost of the equipment, the significant duration of an analysis, and difficulties in screening a large number of samples. Lateral Flow ImmunoAssay (LFIA) attributes are high throughput, high specificity and sensitivity, as well as low cost and simplicity. An LFIA test strip was developed. This LFIA is based on anti-BPA antibodies (from mouse) conjugated with gold nanoparticles as the marker by passive absorption and covalent coupling, deposited in an elaborate pad. To form control and test zones, anti-mouse antibody and a BVA-BSA conjugate were spotted on the nitrocellulose membrane, respectively. Negative samples are revealed by red lines both in the test and control zones, respectively, whereas positive samples produce a single red line only in the control zone. The measurement range is 0.05 – 23 μg/L, visually detected by the naked eye within 5 minutes. In addition, the intensities at the test line can be read by dedicated lateral flow readers (opTrilyzer® and Cube by opTricon, Berlin) for a more precise and documented determination. T2 - EBS2019 CY - Florence, Italy DA - 18.02.2019 KW - BPA KW - LFIA KW - Plastic PY - 2019 AN - OPUS4-47477 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schmidt, Stephan A1 - Hoffmann, Holger A1 - Garbe, L.-A. A1 - Harrer, A. A1 - Steiner, M. A1 - Himly, M. A1 - Schneider, Rudolf T1 - New immunoassay for quantification of diclofenac in water samples N2 - Diclofenac (DCF) is a widely used drug against fever, inflammation, pain, and rheumatic diseases. An average of 70 % of the ingested diclofenac is excreted in the urine. Thus, 63 tons per year are introduced into the water cycle in Germany. Due to insufficient removal of diclofenac in wastewater treatment plants, residues of diclofenac can be found in surface water and sometimes in drinking water. In order to establish an immunoassay, an anti-DCF antibody was required. Some antibody developments have been reported, based on an immunogen resulting from direct coupling of diclofenac to proteins via the carboxylic function. Finally, we used a monoclonal antibody, clone F01G21. T2 - Tag der Biotechnologie der TU Berlin CY - Berlin, Germany DA - 13.07.2018 KW - Wasserproben KW - Arzneimittel KW - Antikörper KW - ELISA PY - 2018 AN - OPUS4-45475 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -