TY - CONF A1 - Ecke, Alexander A1 - Westphalen, Tanja A1 - Retzmann, Anika A1 - Schneider, Rudolf T1 - The Fate of the Antibiotic Amoxicillin in the Aquatic Environment N2 - Contamination of the environment with antibiotics is of great concern as it promotes the evolution of antimicrobial resistances. In case of amoxicillin (AMX) in the aquatic environment, further risk arises from hydrolysis products (HPs) which can cause allergy. To assess these risks, a comprehensive investigation and understanding of the degradation of AMX is necessary. We investigated the hydrolysis rate of AMX in different types of water as well as the influence of temperature and irradiation. The content of the heavy metal ions copper and zinc was found to be crucial for the hydrolysis rate of AMX and stability of HPs. Eventually, a new degradation pathway for AMX could be elaborated and confirmed by tandem mass spectrometry (LC-MS/MS). T2 - Berliner Chemie in Praxis Symposium CY - Berlin, Germany DA - 07.10.2022 KW - Hydrolysis KW - Amoxicillin KW - LC-MS/MS KW - ICP-MS PY - 2022 AN - OPUS4-56026 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - An Immunosensor for Pharmaceutical Contaminants in Water Supply N2 - Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast & cost-effective on-site analyses. Magnetic bead-based immunoassays (MBBAs) allow for implementation into an immunosensor for online testing. Particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically (chronoamperometry) on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes. T2 - 11th SETAC Young Environmental Scientists Meeting CY - Online meeting DA - 07.03.2022 KW - Immunosensor KW - Magnetic Beads KW - Water Analysis KW - Electrochemistry KW - Microfluidics PY - 2022 AN - OPUS4-54432 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - An Immunosensor for Pharmaceutical Contaminants in Drinking Water N2 - Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast & cost-effective on-site analyses. Magnetic bead-based immunoassays (MBBAs) allow for implementation into an immunosensor for online testing. Particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically (chronoamperometry) on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes. T2 - JCF Frühjahrssymposium 2022 CY - Hannover, Germany DA - 23.03.2022 KW - Immunosensor KW - Electrochemistry KW - Amoxicillin KW - Diclofenac KW - Magnetic Beads PY - 2022 AN - OPUS4-54627 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Bohm, Konrad A1 - Bell, Jérémy A1 - Schneider, Rudolf T1 - An Immunosensor for Pharmaceutical Contaminants in Water Supply N2 - Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast & cost-effective on-site analyses. Magnetic bead-based immunoassays (MBBAs) allow for implementation into an immunosensor for online testing. Particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically (chronoamperometry) on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes. T2 - Analytica Conference CY - München, Germany DA - 21.06.2022 KW - Immunosensor KW - Magnetic Particles KW - Microfluidics KW - Amperometry KW - Diclofenac KW - Amoxicillin PY - 2022 AN - OPUS4-55381 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - Im Vorhaben „MARKERIA - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA)“ geht es um die Bestimmung von Carbamazepin, Diclofenac, Koffein und einer endogenen Gallensäure als potentielle Marker für die Kalibrierung der SARS-CoV-2 Quantifizierung im Abwasser. Der Vortrag wurde gehalten im Rahmen der 22. Sitzung des Begleitkreises des übergeordneten Projektes ESI-CorA. Er gab den Projektstand wieder, den die BAM bis dahin erzielt hatte. Die Marker waren in sämtlichen untersuchten Abwasser-Zulaufproben enthalten und zeigten spezifische Trends. Weitere Korrelationen müsse die weitergehende Datenauswertung ergeben.A T2 - 22. Sitzung des Begleitkreises des Projektes ESI-CorA CY - Online meeting DA - 13.02.2023 KW - Wastewater-based epidemiology KW - ELISA KW - Abwasser KW - Antikörper KW - Antibody PY - 2023 AN - OPUS4-59205 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ecke, Alexander A1 - Bohm, Konrad A1 - Schneider, Rudolf T1 - Magnetpartikelbasierte Immunoassays als vielseitiges Werkzeug für die Umweltanalytik N2 - Der Bedarf nach schnellen und kostengünstigen Analysemethoden in der (Trink-)Wasseranalytik steigt mit dem zunehmenden Eintrag pharmazeutisch aktiver Substanzen in die Umwelt. Insbesondere die Kontamination mit Antibiotika erscheint neben ökotoxikologischen Effekten vor allem in Hinblick auf die Evolution resistenter Keime im Wasser besorgniserregend. Wir berichten hier über die Entwicklung eines magnetpartikelbasierten Immunoassays (MBBA) zur Detektion des Breitbandantibiotikums Amoxicillin (AMX) in Wasserproben, der im Vergleich zum entsprechenden Enzyme-linked Immunosorbent Assay (ELISA) eine wesentlich kürzere Analysendauer sowie einen breiteren Messbereich mit niedrigerer Nachweisgrenze aufweist. Das angewendete Assayprinzip ist dabei leicht auf weitere Analyten übertragbar und ermöglicht die Implementierung in einen Immunosensor. KW - Magnetpartikel KW - Amoxicillin KW - Immunoassay KW - Wasser KW - Antikörper PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-527366 UR - https://www.gdch.de/netzwerk-strukturen/fachstrukturen/umweltchemie-und-oekotoxikologie/publikationen/mitteilungen/archiv/2021.html SN - 1618-3258 VL - 27 IS - 2 SP - 48 EP - 51 PB - Gesellschaft Deutscher Chemiker (GDCh) CY - Frankfurt/Main AN - OPUS4-52736 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - Magnetpartikelbasierte Immunoassays für die Online-Trinkwasseranalyik N2 - Wir berichten hier über die Entwicklung plattenbasierter Magnetpartikel-Immunoassays für die Bestimmung des Breitbandantibiotikums Amoxicillin und des nicht-steroidalen Antirheumatikums Diclofenac im Trinkwasser. Für die beiden Analyten wurden unterschiedliche Ansätze entwickelt, mit partikelgebundenen Antikörpern für AMX und Analyt-gekoppelten Partikeln für DCF, die die simultane Detektion beider Kontaminanten im nanomolaren Konzentrationsbereich erlauben. T2 - Interdisziplinäres Doktorandenseminar des AK Prozessanalytik CY - Online meeting DA - 21.09.2020 KW - Magnetpartikel KW - Immunoassay KW - Trinkwasseranalytik KW - Diclofenac KW - Amoxicillin PY - 2020 AN - OPUS4-51345 LA - mul AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Fuhry, E. A1 - Schneider, Rudolf A1 - Raysyan, Anna A1 - Wehrhold, M. A1 - Neubert, T. J. A1 - Balasubramanian, K. T1 - Separation and detection of antioxidants using a graphene based electrochemical sensor platform N2 - CVD-Graphene is ideally suited as an active element for electrical and electrochemical sensing platforms, since it provides for high sensitivity and low detection limit. Electrochemical sensors provide a simple way to measure analytes in fluids with high sensitivity without the need for complex instrumentation. Due to These aspects, graphene sensors are interesting for a wide range of applications in bio analytics. Antioxidants are natural or synthetic compounds, which inhibit oxidation processes and hence often find use as preservatives. It has been suggested that the long-term consumption of diets rich in antioxidants is associated with a lower risk of developing diseases linked to oxidative stress. Gallic acid and ascorbic acid are representatives of antioxidants. We have developed a graphene-based sensor platform to separate and subsequently detect antioxidants. Graphene exhibits low detection limits for ascorbic acid and polyphenols. In this study, the sensing of gallic acid and ascorbic acid on bare CVD graphene in various solvents was investigated by Square Wave Voltammetry. Furthermore, the separation and detection of the bioanalytes by deploying different membranes were evaluated. T2 - Chem2dmat2019 CY - Dresden, Germany DA - 03.09.2019 KW - Electrochemical sensor KW - CVD-Graphene PY - 2019 AN - OPUS4-49932 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Paula A1 - Carl, Peter A1 - Schneider, Rudolf T1 - A Rapid Test for Leaching Studies of Biocides from Building Materials N2 - Regardless of its ban in agricultural use, the triazine derivative terbutryn is still used as a biocide against biofouling in building materials, such as façade colors and roof tiles. From there, it is washed off and may contaminate soil and surface waters, where it shows ecotoxicological behavior. For understanding of these leaching processes, analytical methods that can detect terbutryn in traces are necessary. In contrast to separation methods (Gas (GC) or liquid chromatography (LC)) coupled to mass spectrometry (MS), enzyme immunoassays (EIAs) have proven to be adequate tools for environmental monitoring. Compared to GC-or LC-MS, they are cheap, require only limited time and experimental expertise and enable high throughput. For quantification of terbutryn, EIAs have been established, with a limit of detection (LOD) of 50 ng L-1. For leaching experiments however, a lower LOD would be advantageous. For detection of terbutryn we developed a direct competitive ELISA based on a monoclonal antibody: Terbutryn and an enzyme-terbutryn conjugate (“tracer”) compete for the binding sites of the immobilized antibody. The bound tracer converts a colourless substrate into a blue-coloured product, which can be detected via spectrophotometry. For quantification of terbutryn, a sigmoidal calibration curve is used. For establishing of the EIA, a new tracer based on a terbutryn surrogate (“hapten”) was synthesized. After optimization of the experimental conditions, the immunoassay exhibited a more than ten-fold increased sensitivity (LOD: 3 ng L-1), high stability against sample compounds (Ca2+, NaCl) and lower cross-reactivities against structurally related compounds, than reported before. Analysis of spiked samples was possible with high precision (inter-assay CV: < 10 %) and accuracy (recoveries from spiked samples: 89 % ± 10%). We therefore propose it as quick and reliable method for leaching studies of terbutryn out of building materials. T2 - Adlershofer Forschungsforum CY - Berlin, Germany DA - 11.11.2019 KW - ELISA KW - Terbutryne KW - Hapten Immunoassay KW - Triazine herbicide PY - 2019 AN - OPUS4-49595 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - Immunoassay for the determination of amoxicillin in water samples N2 - Herein we present the data for a newly developed immunoassay for the determination of amoxicillin (AMX) in water samples using a commercially available monoclonal anti-AMX antibody. During optimization and testing of the assay, we observed a significant decrease of the C-value (IC50) of the ELISA calibration curves with aged standard solutions. Running the assay with standard solutions of synthesized AMX hydrolysis products revealed that this decreased C-value could be attributed mainly to the hydrolysis product amoxicilloic acid. Therefore, the limit of detection of the ELISA for AMX determination in water could be enhanced by either hydrolyzing the samples or testing for amoxicilloic acid as marker substance. T2 - Adlershofer Forschungsforum CY - Berlin, Germany DA - 11.11.2019 KW - Amoxicillin KW - Immunoassay KW - Water Analysis KW - Hydrolysis products PY - 2019 AN - OPUS4-49588 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - Immunanalytische Bestimmung von β-Lactam-Antibiotika und deren Hydrolyseprodukten im Trinkwasser N2 - Die speziellen Herausforderungen der immunanalytischen Bestimmung von β-Lactam-Antibiotika in (Trink-)Wasserproben werden anhand eines neu entwickelten ELISA (enzyme-linked immunosorbent assay) für das Breitbandantibiotikum Amoxicillin diskutiert. Es konnte gezeigt werden, dass die Hydrolyse des Analyten einen positiven Einfluss auf die Erkennung durch den Antikörper hat. Das entsprechende Hydrolyseprodukt, zu dem der Antikörper folglich eine höhere Affinität aufweist, wurde identifiziert und ein entsprechendes Verhalten wurde auch für andere β-Lactam-Antibiotika aus der Gruppe der Penicilline beobachtet. Eine Strategie für die Analyse von Wasserproben hinsichtlich Kontamination mit den hier betrachteten β-Lactamen und deren Hydrolyseprodukten wurde ausgearbeitet. T2 - Wasser 2020 - Jahrestagung der Wasserchemischen Gesellschaft CY - Meeting was canceled DA - 18.05.2020 KW - ELISA KW - Immunoassay KW - Antibiotika KW - Hydrolyseprodukte KW - Amoxicillin PY - 2020 UR - https://www.wasserchemische-gesellschaft.de/images/pdf_veranstaltungen/2020/Wasserchemische_Gesellschaft_Tagungsband_Wasser_2020_s.pdf SP - 466 EP - 470 AN - OPUS4-51061 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braun, Ulrike A1 - Selinka, Hans-Christoph A1 - Bannick, Claus Gerhard A1 - Fretschner, Till A1 - Kerndorff, Alexander A1 - Marquar, Natalie A1 - Obermaier, Nathan A1 - Schneider, Beater A1 - Schneider, Rudolf A1 - Konthur, Zoltán T1 - Von der Kläranlage in das Labor N2 - Im Vorhaben „MARKERIA - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA)“ geht es um die Bestimmung von Carbamazepin, Diclofenac, Koffein und einer endogenen Gallensäure als potentielle Marker für die Kalibrierung der SARS-CoV-2 Quantifizierung im Abwasser. Es ist ein Teilvorhaben des übergeordneten Projektes ESI-CorA und wird von der BAM im Auftrag des Umweltbundesamtes durchgeführt. Der Vortrag wurde gehalten im Rahmen der Abschlussveranstaltung des Projektes ESI-CorA. Er gab den Projektstand wieder, den UBA und BAM bis dahin erzielt hatten, das SARS-CoV-2 Virus und die anthropogenen Marker in 20 Kläranlagenzuläufen zu bestimmen und miteinander zu korrelieren. T2 - Abschlussveranstaltung des EU-Projekts ESI-CorA CY - Karlsruhe, Germany DA - 22.03.2023 KW - Wastewater-based epidemiology KW - Abwasser KW - ELISA KW - Coronavirus KW - COVID-19 PY - 2023 AN - OPUS4-59204 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - 2. Projekttreffen mit dem Umweltbundesamt zum Projekt MARKERIA (VH1802). Ziel des Projektes ist es, die Eignung der Bestimmung eines oder mehrerer der genannten anthropogenen Marker im Abwasser zur Volumenkorrektur („Kalibration“) im SARS-CoV-2-Monitoring von Abwässern auf der Basis eines von der BAM zu erhebenden Messdatensatzes zu evaluieren. T2 - 2. Projekttreffen mit dem UBA zum Projekt MARKERIA (VH1802) CY - Berlin, Germany DA - 25.11.2022 KW - Biosensoren KW - Immunoassay KW - SARS-CoV-2 PY - 2022 AN - OPUS4-57112 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Konthur, Zoltán A1 - Ecke, Alexander A1 - Kerndorff, A. A1 - Hoffmann, Kristin A1 - Andrle, Paul A1 - Kurt, Lisa B. A1 - Lukas, M. A1 - Bannick, C. G. A1 - Braun, U. A1 - Schneider, Rudolf T1 - Deutschlandweite Bestimmung anthropogener Marker im Abwasser N2 - Die von der Europäischen Kommission im Jahr 2021 empfohlene, systematische Abwasserüberwachung mittels PCR-Techniken wird als ein wirksames Früh-warnsystem für künftige Pandemien durch Viren und andere Krankheitserreger betrachtet. Ein generelles Problem bei jeder quantitativen Messung von Parametern im Abwasser ist die starke Schwankung des Abwasservolumens und seiner Zusammensetzung im Tages- und Jahreszeitenverlauf. Um die Konzentrationsbestimmungen von Krankheitserregern im Abwasser in Korrelation mit menschlichen Einträgen setzen zu können, könnten sog. "anthropogene Marker" eine herausragende Rolle spielen, deren Eintrag ins Abwasser menschlichen Ursprungs sind. Im Projekt werden mittels ELISA die Konzentrationen ausgewählter anthropogener Marker in deutschlandweit gesammelten Abwasserproben erhoben und analysiert. T2 - Spurenstoffe und Krankheitserreger im Wasserkreislauf CY - Frankfurt/Main, Germany DA - 27.03.2023 KW - Carbamazepin KW - Koffein KW - Diclofenac KW - Isolithocholsäure PY - 2023 AN - OPUS4-57718 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Screening the environment for emerging pollutants by antibody-based methods N2 - Immunoanalytical methods are excellent tools for analysis of the environment with respect to pollutants T2 - ENEQUI Encontro Nacional dos Estudantes de Química CY - Lisbon, Portugal DA - 22.03.2019 KW - Immunoassay KW - Schadstoffe KW - Antikörper PY - 2019 AN - OPUS4-48321 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Raysyan, Anna A1 - Schneider, Rudolf T1 - An immunochromatographic assay for the determination of bisphenol a releases from plastic materials N2 - Background. The worldwide increase in the use of bisphenol A (BPA), a monomer commonly used to manufacture polycarbonate plastic products and the epoxy resins that line metal cans, has resulted in an increased human exposure because both products come in contact with food and beverages. Since BPA is an endocrine disruptor excessive exposure could affect human reproductive function. In response, various countries have adopted a range of measures, from voluntary reductions to outright bans of BPA in some products, mostly in baby bottles and containers for canned foods. Nonetheless, consumers would certainly appreciate a quick test for their own assessment of contaminations by bisphenol A. Methods. An immunochromatographic test strip was developed. This lateral-flow immunoassay (LFIA) is based on anti-BPA antibodies (from mouse) conjugated with gold nanoparticles as the marker, deposited in an elaborate pad. To form control and test zones, anti-mouse antibody and a BPA-BSA conjugate were spotted on the nitrocellulose membrane, respectively. Results. Negative samples are revealed by red lines both in the test and control zones, respectively, whereas positive samples produce a single red line only in the control zone. The limit of detection (LOD) was found to be 0.05 µg/l, visually detected by the naked eye within 5 minutes. In addition, the intensities at the test line can be read by dedicated lateral flow readers (opTrilyzer® and Cube by opTricon, Berlin) for a more precise and documented determination. Conclusion. Pregnancy-test like strips in combination with hand-held readers provide a simple and versatile tool to determine the presence of bisphenol A quantitatively, making it a very attractive analytical approach especially for on-site applications at critical points, such as environmental analysis, consumer protection, and materials testing. T2 - International Biotech Innovation Days CY - Senftenberg, Germany DA - 22.05.2018 KW - Immunoassay KW - BPA KW - LFIA KW - Plastic PY - 2018 AN - OPUS4-46752 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - Vortrag zum Kick-off des Projektes. Ziel des Projektes ist es, die Eignung der Bestimmung eines oder mehrerer der genannten anthropogenen Marker im Abwasser zur Volumenkorrektur („Kalibration“) im SARS-CoV-2-Monitoring von Abwässern auf der Basis eines von der BAM zu erhebenden Messdatensatzes zu evaluieren. T2 - Kick-off Meeting zum Projekt MARKERIA (VH1802) CY - Berlin, Germanyy DA - 01.04.2022 KW - Biosensoren KW - Immunoassay KW - SARS-CoV-2 PY - 2022 AN - OPUS4-57109 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Environmental Analysis at BAM N2 - The talk is to introduce BAM, the Department of Analytical Chemistry; Reference Materials and the Division of Environmental Analysis and its works to the audience. T2 - Seminar des Centre for Environmental and Marine Studies (CESAM) der Universität Aveiro CY - Aveiro, Portugal DA - 12.09.2022 KW - Immunoassay KW - Biosensor KW - Antibody PY - 2022 AN - OPUS4-56784 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Insights at the point-of-need via immunoanalytical methods N2 - A vast number of emerging pollutants is being detected in the environment. Another lingering problem are health-threatening contaminants, such as mycotoxins, that deteriorate food and feed, and the pathogens themselves. Analytical methods, suitable for trace analysis, are needed that are desirably also fast, inexpensive and, if possible, robust and portable. It is set out, how immunoana-lytical, i.e., antibody-based methods, which are elaborated in a broad range of formats, can be profitably used to gain insights on the distribution and concentration trends of the target analytes at the point-of-need. T2 - Kolloquium der Fakultät für Pharmazie der Universität Porto CY - Porto, Portugal DA - 01.07.2022 KW - Immunoassay KW - Sensor KW - Antibody PY - 2022 AN - OPUS4-56781 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Anthropogenic markers quantified by rapid immunochemical methods – what can their occurrence in wastewater, surface water, and drinking water tell us? N2 - In urban waters, a multitude of organic micropollutants, often termed emerging pollutants, has been found over the last decades. Analytical methods suitable for trace analysis are needed that are desirably also fast, inexpensive and, if possible, robust and portable. Immunoanalytical, i.e., antibody-based, methods which are available in a broad range of formats, can be profitably used here to screen for the distribution and to monitor the trends of concentration levels of contaminants of emerging concern in the environment. Some of these formats are single-analyte but high-throughput methods. To use them wisely, indicator substances, sometimes called anthropogenic markers, should be selected and used in screening approaches, i.e., as indicators for contamination and the pre-selection of samples at which to have a closer look by multiplex methods like LC-MS/MS. Other methods are suitable to be performed on portable instrumentation in the field (on-site) or in facilities such as wastewater treatment plants for on-line monitoring of the treatment and elimination process. Furthermore, array technologies have been established that allow for parallel (multiplex) analysis of several analytes of interest. The microtiter-plate based ELISA (Enzyme-linked Immunosorbent Assay) is the method of choice for the analysis of a large number of samples [1]. ELISAs are available to monitor for anthropogenic markers such as the antiepileptic carbamazepine, the analgesic diclofenac, the antihistaminic cetirizine, the steroid hormone estrone, the antimicrobial sulfamethoxazole, psychoactive caffeine and cocaine, the priority pollutant bisphenol A, and the bile acid isolithocholic acid. For on-site screening and monitoring, simpler formats, like mix-and-read assays, e.g., the Fluorescence Polarization Immunoassay (FPIA) [2] or Lateral-flow Immunoassays (LFIA) [3] are more suitable tools, the latter based on dipsticks or little cassettes, with which users have become very familiar during the COVID-19 pandemic via rapid antigen tests. The suitability of multi-analyte formats such as immunomicroarrays depends on the choice of a signal-producing system that provides small uncertainties and good reproducibility of the measurements. Bead-based (“suspension”) arrays, read out in flow cytometers, are a powerful platform for multiplex assays [4]. Electrochemical formats, run on portable devices, provide additional advantages as no light source is required. They are most promising for stand-alone analysers and biosensors [5]. The speed, low cost and on-site capabilities of these methods allow to gather a lot more data on anthropogenic compounds which enables to quantify inputs, differences in degradation power of elimination processes, dilution phenomena and a more precise image of individual water cycles which is demonstrated by several examples. T2 - EuChemS Chemistry Congress ECC8 CY - Lisbon, Portugal DA - 28.08.2022 KW - Biosensor KW - Immunoassay KW - Antibody PY - 2022 AN - OPUS4-56783 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -