TY - CONF A1 - Fontana, Patrick A1 - Miccoli, Lorenzo A1 - Kocadag, R. A1 - Silva, N. A1 - Qvaesching, D. A1 - Kreft, O. A1 - Cederqvist, Ch. ED - Fehling, E. ED - Middendorf, B. ED - Thiemicke, J. T1 - Composite UHPC facade elements with functional surfaces N2 - This paper presents an innovative way to combine an external ultra-high performance concrete (UHPC) supporting layer with an insulation layer of autoclaved aerated concrete (AAC) or cellular lightweight concrete (CLC) to create light-weight façade elements, which are improved in functionality and in energy efficiency. The durability of the façade elements is improved by developing UHPC with self-cleaning properties. One approach is based on the photocatalytic activation of the external UHPC shell by incorporation of TiO2 particles. The second approach consists of the modification of the UHPC surface by micro structuring in combination with the application of water-repellent agents to create durable super hydrophobicity. The current results obtained from laboratory testing are promising and demonstrate the feasibility of the approaches. T2 - HiPerMat 2016 4th International Symposium on Ultra-High Performance Concrete and High Performance Construction Materials CY - Kassel, Germany DA - 09.03.2016 KW - Composite UHPC elements KW - photocatalysis KW - super hydrophobicity KW - self-cleaning KW - autoclaved aerated concrete KW - cellular lightweight concrete PY - 2016 SN - 978-3-7376-0094-1 VL - 27 SP - 159 EP - 160 PB - kassel university press GmbH CY - Kassel AN - OPUS4-36566 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hofmann, Michael A1 - Richter, Matthias A1 - Jann, Oliver T1 - Proficiency test of commercial radon monitors for low level radon exhalation measurements in chambers with air exchange N2 - Compared to the designated European upper reference level of 300 Bq m-3 for indoor radon concentrations, the contribution of building materials appears to be low. Taking into account the recommended limit of 100 Bq m-3 by WHO as well as the increased dose conversion factor, the contribution of building products is quite relevant, especially at low air change rates. Therefore in Germany a maximum contribution of building materials to the indoor radon concentration of 20 Bq m-3 is discussed. Because measurements of low concentrations are associated with high uncertainties, radon exhalation rates are often determined in chambers without air exchange and indoor conditions are extrapolated. This study investigated the suitability of direct low level radon measurement under simulated indoor conditions with commercially available instruments, based on the preliminary EU standard for the determination of the emission of volatile organic compounds (VOC) from building materials in test chambers. Measurements are possible with a relative uncertainty ranging from 7 to 18 %. Four major problems were identified: 1. Detector sensitivity: Two of four instruments were identified to determine low radon concentrations in a sufficiently short measuring time. 2. Thoron discrimination: Thoron was discriminated by aging the sampled air in a hose before the detector. 3. Sampling setup: An external chamber measurement was feasible as long as all main system parts were under slight overpressure. 4. Blank value: For VOC-emission testing ambient air, conditioned by filtration and sorption, is used to generate an air exchange. Regarding radon, this treatment is non-effective. If all instrument background levels have been characterized, the blank value must be measured with a second detector in parallel. T2 - 8th International Conference on Protection against Radon at Home and at Work CY - Prague; Czechia DA - 12.09.2016 KW - Radon exhalation KW - Building materials KW - Radon measurement KW - VOC emission test chamber PY - 2016 AN - OPUS4-37437 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hofmann, Michael ED - Thinová, L. T1 - Proficiency test of commercial radon monitors for low level radon exhalation measurements in chambers with air exchange N2 - Compared to the designated European upper reference level of 300 Bq/m³ for indoor radon concentrations, the contribution of building materials appears to be low. Taking into account the recommended limit of 100 Bq/m³ by WHO as well as the increased dose conversion factor, the contribution of building products is quite relevant, especially at low air change rates. Therefore in Germany a maximum contribution of building materials to the indoor radon concentration of 20 Bq/m³ is discussed. Because measurements of low concentrations are associated with high uncertainties, radon exhalation rates are often determined in chambers without air exchange and indoor conditions are extrapolated. This study investigated the suitability of direct low level radon measurement under simulated indoor conditions with commercially available instruments, based on the preliminary EU standard for the determination of the emission of volatile organic compounds (VOC) from building materials in test chambers. Measurements are possible with a relative uncertainty ranging from 7 to 18%. Four major problems were identified: 1. Detector sensitivity: Two of four instruments were identified to determine low radon concentrations in a sufficiently short measuring time. 2. Thoron discrimination: Thoron was discriminated by aging the sampled air in a hose before the detector. 3. Sampling setup: An external chamber measurement was feasible as long as all main system parts were under slight overpressure. 4. Blank value: For VOC-emission testing ambient air, conditioned by filtration and sorption, is used to generate an air exchange. Regarding radon, this treatment is non-effective. If all instrument background levels have been characterized, the blank value must be measured with a second detector in parallel. T2 - 8th International Conference on Protection against Radon at Home and at Work CY - Prague, Czechia DA - 12.09.2016 KW - Radon exhalation KW - Building materials KW - Radon measurement KW - VOC emission test chamber PY - 2016 UR - http://www.radon2016.cz/download/radon2016-el.pdf?attredirects=0&d=1 SN - 978-80-01-05993-7 SP - 21 EP - 21 PB - České vysoké učení technické v Praze CY - Prague AN - OPUS4-37439 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Piechotta, Christian A1 - Kuhlich, Paul A1 - Maul, R. T1 - Characterization of new disinfection by-products of polycyclic musks (AHTN and HHCB) and their metabolites N2 - The polycyclic musks 6-Acetyl-1,1,2,4,4,7-hexamethyltetraline (AHTN) and 1,3,4,6,7,8-Hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-ɣ-2-benzopyran (HHCB) are widely used fragrances in cosmetics, products of daily care and cleaning products for households and industry. They are in the focus of interest due to their estrogenic potential. Both compounds can be found at low μg/L concentrations in surface water where they are introduced mainly by sewage treatment plants supplied by municipal wastewater. In order to disinfect wastewater in sewage treatment plants or water in swimming pools, chlorine can be added to water as gas or hypochlorite solution. This disinfectant has a high oxidation potential resulting in the formation of disinfection by-products, the estrogenicity and toxicity of which might differ from their precursor. In this work the behaviour of AHTN and HHCB upon treatment with sodium hypochlorite solution was investigated and chlorinated possible disinfection by-products could be identified as well as a carboxylic acids and lactones derived from AHTN and HHCB. The identified species were characterized by GC-MS, LC-MSn and 1H-NMR. The elucidated structures of potential disinfection by-products are verified by X-ray crystallography. Finally the estrogenicity between the parent analytes and generated transformation products were compared. For that task, MCF-7 assay has been chosen for being most relevant to humans. Moreover metabolism studies were carried out using AHTN, HHCB and their disinfection by-products. T2 - ISEB 2016 CY - Barcelona, Spain DA - 30.05.2016 KW - AHTN PY - 2016 AN - OPUS4-38523 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta T1 - Characterization of dye-protein conjugates with optical methods N2 - Fluorophore labeled proteins and antibodies, referred to also as targeted optical probes, present a promising strategy for a variety of applications from fundamental cell-based biological studies to in vivo diagnostics and image guided surgeries in humans. In this respect, design strategies for the preparation of such conjugates from different dyes including analyte-responsive fluorophores are presented as well as their analytical and spectroscopic characterization employing Absorption spectroscopy and steady state and time-resolved fluorometry. Special emphasis is dedicated to the influence of dye hydrophilicity and labeling density on the optical properties and binding behavior of these dye-bioconjugates including their performance in in vitro and in vivo bioimaging studies. T2 - International Workshop “Affinity - Mass spectrometry – New Methods and Application to Protein Therapeutics Development” CY - Rüsselsheim am Main, Germany DA - 14.11.2016 KW - Fluorescence KW - Dye bioconjugates KW - Protein KW - Immunoassay KW - Quality assurance KW - Optical characterization KW - Dye KW - NIR KW - Imaging PY - 2016 AN - OPUS4-38504 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Piechotta, Christian A1 - Fettig, Ina T1 - Traceability for mercury measurements - MeTra N2 - The aim of this work is to develop measurement procedures to provide full traceability and robust uncertainty statements for dissolved elemental mercury (Hg(0)), inorganic mercury (Hg(II)) and methyl-mercury (MeHg) in freshwater and sea water, and inorganic mercury (Hg(II)) and methyl-mercury (MeHg) in biota. Accurate measurements with low uncertainties e.g. <50 % at the EQS level are required to assess concentration levels and trends in these important environmental reservoirs for mercury. In particular this work aims to: Develop and validate primary measurement procedures for Hg speciation (Hg(II), MeHg and Hg(0)) in fresh and sea waters at sub-ng levels in the presence of suspended particular matter and/or colloidal fractions, Apply the developed IDMS procedures in water in order to evaluate possible artefacts associated with sample collection and preparation, preserving speciation and/or correcting for possible post-sampling interconversions, Develop and validate primary measurement procedures for the accurate quantification of Hg(II) and MeHg in biota, using samples from the Federal Environmental Specimen Bank, Apply the developed IDMS procedures in biota in order to evaluate the effect of different treatments for fish sample preparation. T2 - EMRP MeTra Meeting CY - Geel, Belgium DA - 01.02.2016 KW - Methyl mercury PY - 2016 AN - OPUS4-38519 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Maierhofer, Christiane A1 - Kind, Thomas A1 - Köpp, Christian A1 - Helmerich, Rosemarie T1 - CHEF - Cultural heritage protection against flooding N2 - CHEF provides knowledge about flood protection of cultural heritage objects and directly supports the European Floods Directive by giving recommendations on how to assess the vulnerability of monuments. A comprehensive analysis of protective measures BEFORE, DURING and AFTER a flood has been carried out within the project, including technical and administrational measures. An overview of these measures is given, followed by recommendations on how to establish the most effective protective strategies. KW - Cultural heritage KW - Flood protection KW - Flood risks PY - 2011 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-385463 SP - 1 EP - 3 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-38546 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braun, Ulrike T1 - The role of metrological institutions in the pollution by microplastic particles N2 - The presentation gives an overview about the requirement and needs for reproducible microplastic analysis in environment. A special focus is on the comparison of different analysis techniques. T2 - Thermo Fisher Workshop, Dreieich CY - Dreieich, Germany DA - 30.11.2016 KW - Microplastic PY - 2016 AN - OPUS4-38930 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schmidt, Birgit Angelika A1 - Pentzien, Simone A1 - Conradi, Andrea A1 - Krüger, Jörg A1 - Roth, Constanze A1 - Beier, Oliver A1 - Hartmann, Annett A1 - Grünler, Bernd T1 - Decontamination of biocidal loaded wooden artworks by means of laser and plasma processing N2 - Many wooden artworks are contaminated by DDT (dichlorodiphenyltrichloroethane) as a result of a surface treatment by means of Hylotox-59©. The liquid preservative was used until the end of the 80s. DDT crystal structures are formed on the wood surfaces by the "blooming" of chlorine compounds. Contaminated waste wood with natural biocide ageing, gilded and wood carved elements of an old picture frame and wooden samples with paint layers were provided by the Schlossmuseum Sondershausen. The removal of DDT was evaluated employing femtosecond and nanosecond laser radiation and cold atmospheric plasma technique using different working gases (air, nitrogen, and argon). Before laser application, a chlorine measurement is done by X-ray fluorescence (XRF) analysis as reference. After laser processing, the XRF analysis is used again at the same surface position to determine depletion rates. Additionally, a documentation and characterization of the sample surface is done before and after laser and plasma treatment using optical microscopy. For plasma processing with various systems a chlorine measurement is done by gas chromatographic-mass spectrometry (GCMS) analysis. For laser treatment a depletion of the chlorine concentration of 55-70% and 75% was achieved for 1064-nm nanosecond pulses and 800-nm femtosecond pulses, respectively. For the application of 30-fs laser pulses, no crystalline DDT residues remain on the sample surfaces. This holds also for the plasma processing with nitrogen as working gas. T2 - Conference on Lasers in the Conservation of Artworks (LACONA XI) CY - Krakow, Poland DA - 19.09.2016 KW - Decontamination KW - Wood KW - DDT KW - Laser cleaning KW - Plasma treatment PY - 2016 AN - OPUS4-38903 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schoknecht, Ute A1 - Mathies, Helena A1 - Wegner, R. A1 - Uhlig, S A1 - Baldauf, H. A1 - Colson, B T1 - Leaching of biocides from organic coatings N2 - Biocide leaching was investigated for acrylate and vinyl acetate based coatings containing biocides either as liquid formulations or microcapsules. Laboratory and field tests indicate similar relations of results on carbendazim, diuron, terbutryn and OIT from different coatings in laboratory and field experiments. Emissions were higher in EN 16105 laboratory tests than from vertically installed test specimens exposed to weathering, and slower for microencapsulated biocides. Competing processes that cause losses of active substances can occur in both tests, but to a higher degree in field experiments. Emission curves related to runoff water were similar in repeated field experiments. Relative humidity, temperature and global radiation were identified as meteorological factors that affect leaching by complex interaction besides the amount of driving rain. KW - Leaching KW - Biozid KW - Beschichtung PY - 2016 SN - 1230-3321 IS - 4 SP - 3 EP - 9 AN - OPUS4-38909 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Müller, U. A1 - Miccoli, Lorenzo A1 - Fontana, Patrick T1 - A lime-based grouting material for the repair of earthen structures N2 - The repair of earthen structures is not an easy task. Earth as a construction material has comparatively weak mechanical properties. It is also susceptible towards liquid water, were it can lose cohesion very quickly if certain moisture contents are exceeded. Repair concepts of structures built with such materials are therefore challenging. Historical earthen structures often exhibit damages in form of extensive cracking, which may have been caused by static or dynamic loads. Frequently these cracks are insufficiently or inappropriately repaired because of lack of knowledge and / or technology. In particular, the behaviour of crack repair by grouting poses a challenge in earthen materials and demands specific requirements for the grouting mortar, such as low water content, good water retention, low shrinkage. Grouting materials require additional specifications such as compatible strengths and Young’s modulus as well as good adhesion to the earthen substrates. In addition, grouts have to be sufficiently flowable to fill small cracks and voids without segregation or bleeding. Therefore, the rheological behaviour has to be well understood and controlled to gain the desired effects. The repair of cracks in earthen buildings is traditionally done by stuffing manually mortar into the gap. Naturally, this method is only usable for cracks with large widths. Another disadvantage is that cracks going through thicker walls cannot be completely reached by the tools used for stuffing the mortar into the crack. Lime based grouts for earthen materials were usually used for re-attachment of plasters but less for structural reasons. Due to the nature of earthen materials grouts based on formulated or hydraulic lime (according to the definition in EN 459-1) have to meet considerable demands on a variety of properties, which are related to compatibility, durability and in particular to the ability of being injected. The goal of this study was to create a grout, which can be used to re-establish structural continuity in cracked earthen masonry or other massive earth walls (rammed earth and cob) with the focus on grouting cracks. The grout material was based on hydrated lime (calcium hydroxide) with additions of pozzolana and lime stone filler. T2 - Lehm 2016 - 7th International Conference on Building with Earth CY - Weimar, Germany DA - 12.11.2016 KW - Building Materials KW - Grout mortar KW - Hydraulic lime KW - Earth construction KW - Repair KW - Historical buildings PY - 2016 SP - 1 EP - 8 PB - Eigenverlag Dachverband Lehm e. V. CY - Weimar AN - OPUS4-38825 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Adam, Christian T1 - SUSAN - Sustainable and safe re-use of municipal sewage sludge for nutrient recovery N2 - The SUSAN-project aimed at developing a sustainable and safe strategy for nutrient recovery from sewage sludges using thermal treatment. Mono-incineration of the sludges completely destructs the organic pollutants in a first step. The incineration residues are ashes with a high phosphorus content that still contain heavy metal compounds above the limits for agricultural use. Furthermore, phosphorus in the ashes exhibits low bioavailability - a disadvantage in farming. Therefore, in a second thermochemical step heavy metals are removed and phosphorus transferred into mineral phases available for plants. The principle of the SUSAN-project is the separation of the small heavy metals fraction from the ashes to receive a phosphorus rich product suited as raw material for fertilisers that also contains SiO2, CaO, Al2O3, Fe2O3, MgO and K2O. The thermochemical process is based on evaporation of volatile heavy metal chlorides at 850 - 1000 °C. The sewage sludge ashes are mixed with chlorine donors such as MgCl2 or KCl, grained and treated in a thermal aggregate e.g. a rotary furnace. At the same time a transfer of non soluble P-compounds into Mineral phases available for plants takes place. Besides technological development of the thermochemical process, the products fertilisation performance, the product design, the market for the products and the sustainability of the whole process chain are emphasised in the SUSAN-project. KW - Nutrient recovery KW - Sewage sludges KW - Sludge disposal KW - Sustainability KW - Environmental pollution PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-391607 UR - http://cordis.europa.eu/result/rcn/46724_en.html SP - 1 EP - 4 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-39160 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Moghaddam, J. A. A1 - Boehringer, N. A1 - Burdziak, A. A1 - Kunte, Hans-Jörg A1 - Galinski, E. A. A1 - Schäberle, T. F. T1 - Different strategies of osmoadaptation in the closely related marine myxobacteria Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1 N2 - Only a few myxobacteria are known to date that are classified as marine, owing to their salt dependency. In this study, the salt tolerance mechanism of these bacteria was investigated. To this end, a growth medium was designed in which the mutated Escherichia coli strain BKA13 served as sole food source for the predatory, heterotrophic myxobacteria. This enabled measurement of the osmolytes without any background and revealed that the closely related strains Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1 developed different strategies to handle salt stress. Ple. pacifica SIR-1, which was grown between 1 and 4% NaCl, relies solely on the accumulation of amino acids, while Enh. salina SWB007, which was grown between 0.5 and 3% NaCl, employs, besides betaine, hydroxyectoine as the major compatible solute. In accordance with this analysis, only in the latter strain was a locus identified that codes for genes corresponding to the biosynthesis of betaine, ectoine and hydroxyectoine. KW - Osmoregulation KW - Myxobacteria KW - Betaine KW - Ectoine PY - 2016 DO - https://doi.org/10.1099/mic.0.000250 VL - 162 IS - 4 SP - 651 EP - 661 AN - OPUS4-39115 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Kraft, Marko A1 - Würth, Christian A1 - Kaiser, Martin A1 - Muhr, V. A1 - Hirsch, T. T1 - Recent insights in the spectroscopic properties of upconversion nanoparticles N2 - Lanthanide-doped up-converting nanoparticles (UCNPs) are promising reporters for medical diagnostics and bioimaging, which are excited in the near infrared (NIR) by multiphoton absorption processes, and show multiple narrow emission bands in the visible (vis) and NIR, long luminescence lifetimes in the μs range, and excellent photostability. Current limitations present their relative low absorption cross sections and low fluorescence efficiencies, with the latter being affected by particle size, surface chemistry, and microenvironment, particularly water. Here, we present results from systematic studies of the excitation power density dependent upconversion luminescence spectra, intensities/intensity ratios of the individual emission bands, slope factors, and quantum yields of UCNPs of varying size, dopant concentration, and surface chemistry in different microenvironments as well as the up- and downconversion luminescence decay kinetics of the different emission bands. Moreover, first studies of the energy transfer from UCNPs to surface-bound organic dyes acting as fluorescence acceptors are shown. Based upon these measurements, fluorescence deactivation channels are identified and spectroscopic parameters for the screening of material performance are derived. T2 - Spie Photonics west 2017 CY - San Francisco, USA DA - 28.01.2017 KW - Upconverting nanoparticles KW - Size KW - FRET KW - Fluorescence KW - Absolute fluorescence quantum yield KW - Fluorescence decay kinetics KW - Power density dependence PY - 2017 AN - OPUS4-39075 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - McMahon, Dino Peter T1 - Blurred boundaries between society and individuality: using immunity to understand a major evolutionary transition N2 - A fundamental yet seemingly contradictory aspect of biological individuals is their capacity to remain the same while changing through time. This self-regulatory ability is facilitated by a number of factors that are shared by all complex multicellular life-forms. One such essential trait is an effective immune system. Here, I use concepts of individuality and immunity to explore the blurred line between society and individual in termite “superorganisms”. I discuss our current understanding of the breadth and mechanistic underpinnings of collective immunity in termites, before going on to explore the evolution of the immune system during the transition from a cockroach-like ancestor to a fully-fledged termite society. I end by discussing the possibly crucial role played by immunity during the emergence of eusociality, and in transitions in organismal complexity generally. T2 - BIO-Seminar, University of Copenhagen CY - Copenhagen, Denmark DA - 27.02.2018 KW - Termite KW - Evolution KW - Social KW - Immunity PY - 2017 AN - OPUS4-44242 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - McMahon, Dino Peter T1 - The evolution of termite immunity N2 - The evolution and mechanistic underpinnings of termite immunity are not well understood. Given the intimate relationship between sociality and disease, this represents a major gap in understanding, particularly since termites represent the only animal group outside of Hymenoptera to have evolved such advanced forms of social living. We explore the evolution of termite immunity by conducting qualitative and quantitative transcriptomic analyses from twelve cockroach and termite species occurring along a gradient of sociality. We incorporate lineages spanning the origin of eusociality, and include species from a key transitional subsocial group: the wood roaches. Firstly, we analyze immune characteristics in the context of termite phylogeny. Here, we examine the evolution of immune traits during the transition to eusociality, and explore the extent to which immune adaptations are associated with evolutionary shifts in social complexity and ecology. Secondly, we conduct a quantitative analysis of responses to a common immune stimulus to examine the role of both sociality and caste in immunity. Here, we test the hypothesis that the evolution of division of labour – and associated increases in investment into costly altruistic (immune) traits – occurred at the expense of individual immunity in the sterile caste. T2 - Congress of the European Society for Evolutionary Biology CY - Groningen, Netherlands DA - 20.08.2017 KW - Evolution KW - Termite KW - Immunity PY - 2017 AN - OPUS4-44243 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - McMahon, Dino Peter T1 - The evolution of termite immunity N2 - The evolution and mechanistic underpinnings of termite immunity are not well understood. Given the intimate relationship between sociality and disease, this represents a major gap in understanding, particularly since termites represent the only animal group outside of Hymenoptera to have evolved such advanced forms of social living. We explore the evolution of termite immunity by conducting qualitative and quantitative transcriptomic analyses from twelve cockroach and termite species occurring along a gradient of sociality. We incorporate lineages spanning the origin of eusociality, and include species from a key transitional subsocial group: the wood roaches. Firstly, we analyze immune characteristics in the context of termite phylogeny. Here, we examine the evolution of immune traits during the transition to eusociality, and explore the extent to which immune adaptations are associated with evolutionary shifts in social complexity and ecology. Secondly, we conduct a quantitative analysis of responses to a common immune stimulus to examine the role of both sociality and caste in immunity. Here, we test the hypothesis that the evolution of division of labour – and associated increases in investment into costly altruistic (immune) traits – occurred at the expense of individual immunity in the sterile caste. T2 - Ecological Immunology Workshop 2017 CY - Blossin, Brandenburg, Germany DA - 28.08.2017 KW - Evolution KW - Termite KW - Immunity PY - 2017 AN - OPUS4-44244 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - He, Shulin A1 - McMahon, Dino Peter A1 - Lokatis, S. T1 - Defend and disinfect: a flexible role for soldiers in termite society N2 - The first sterile caste to evolve in termites was the soldier. While the importance of soldiers to termite society defense has long been recognized, the contribution of soldiers to other societal functions, such as colony immunity, is less well understood. We explore this issue by examining the role of soldiers in protecting nestmates against pathogen infection. Even though they are unable to engage in grooming behavior, we find that the presence of soldiers of the Darwin termite, Mastotermes darwiniensis, significantly improves the survival of nestmates following entomopathogenic infection. We also show that the copious exocrine oral secretions produced by Darwin termite soldiers contain a high concentration of proteins involved in digestion, chemical biosynthesis, and immunity. The oral secretions produced by soldiers are sufficient to protect nestmates against infection, and they have potent inhibitory activity against a broad spectrum of microbes. Our findings support the view that soldiers may play an important role in colony immunity, and broaden our understanding of the possible function of soldiers during the origin of soldier-first societies. T2 - Congress of the European Society for Evolutionary Biology CY - Groningen, Netherlands DA - 20.08.2018 KW - Termite KW - Immunity KW - Social PY - 2017 AN - OPUS4-44248 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - He, Shulin A1 - Lokatis, S. A1 - McMahon, Dino Peter T1 - Defend and disinfect: a flexible role for soldiers in termite society N2 - The first sterile caste to evolve in termites was the soldier. While the importance of soldiers to termite society defense has long been recognized, the contribution of soldiers to other societal functions, such as colony immunity, is less well understood. We explore this issue by examining the role of soldiers in protecting nestmates against pathogen infection. Even though they are unable to engage in grooming behavior, we find that the presence of soldiers of the Darwin termite, Mastotermes darwiniensis, significantly improves the survival of nestmates following entomopathogenic infection. We also show that the copious exocrine oral secretions produced by Darwin termite soldiers contain a high concentration of proteins involved in digestion, chemical biosynthesis, and immunity. The oral secretions produced by soldiers are sufficient to protect nestmates against infection, and they have potent inhibitory activity against a broad spectrum of microbes. Our findings support the view that soldiers may play an important role in colony immunity, and broaden our understanding of the possible function of soldiers during the origin of soldier-first societies. T2 - 22nd Graduate Meeting in Evolutionary Biology of the German Zoological Society CY - Plön, Germany DA - 12.09.2017 KW - Termite KW - Social KW - Immunity PY - 2017 AN - OPUS4-44249 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - He, Shulin A1 - McMahon, Dino Peter T1 - Differentiation of immune response with castes in termites N2 - Effective immunity is essential for maintaining insect society integrity but the mechanisms that connect immunity at the level of the individual to the level of society are poorly understood. Here, we begin to resolve this issue by addressing the interaction between individual immunity and caste identity in the termite Neotermes castaneus. We characterize the transcriptomic responses of different castes to a general immune challenge by sequencing mRNAs from false workers, soldiers and reproductive following injection with a cocktail of heat-killed pathogens. We found many more genes to be differentially regulated in reproductives (1420 and 398 genes down- and upregulated respectively) than soldiers (90 and 108 genes) or false workers (135 and 71 genes). We go on to conduct a quantitative analysis of the specific changes to immune regulation across the castes following pathogen exposure, before discussing the implications of these findings for our understanding of the evolution of immunity in eusocial termites. T2 - Ecological Immunology Workshop 2017 CY - Blossin, Brandenburg, Germany DA - 28.08.2017 KW - Termite KW - Immunity KW - Caste PY - 2017 AN - OPUS4-44251 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - He, Shulin A1 - McMahon, Dino Peter T1 - Differentiation of immune response with castes in termites by de novo transcriptome sequencing N2 - Eusociality is one major evolutionary transitions, during which the division of labor is considered as the hallmark feature. This character of evolution, also interpreted as task specialization, is associated with multiple morphological, physiological and behavioral adaptations required for brood care, foraging, and defense. With these adaptations which ultimately cause the formation of castes in insect sociality (mostly in termites, ants, some bees and wasps), while whether immune system is related to eusociality in termites is rare understood. We hypothesis that immune system in termite sociality had also differentiation along with the formation of castes. We address this issue by exploring the immune responses of different castes with de novo transcriptome sequencing. After injection with cocktail of heat-killed pathogens (Pseudomonas entomophila, Bacillus thuringiensis, Saccharomyces cerevisiae), the mRNAs from false workers, soldiers and reproductive of Neotermes canstaneus were sequenced. We found much more genes are regulated (1420 genes downregulated and 398 genes upregulated) in reproductives compared to soldiers (90 genes downregulated and 108 genes upregulated) and false workers (135 genes downregulated and 71 genes upregulated). In these upregulated genes, reproductives had 54 same genes as soldiers, while only 7 same genes as workers which almost is same number between soldiers and workers. In the downregulated genes, reproductives shared 50 and 41 same genes as soldiers and workers, respectively. Besides, soldiers and workers had 29 same downregulated genes. From these findings, it is indicated that the immune system differentiates with division of labor during eusociality in termites. T2 - V. Central European Meeting of IUSSI 2017 CY - Kloster Schöntal, Deutschland DA - 09.10.2017 KW - Immunity KW - Social KW - Termite KW - Transcriptome PY - 2017 AN - OPUS4-44252 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - He, Shulin A1 - McMahon, Dino Peter T1 - The evolution of immunity during the transition to eusociality N2 - The evolution and mechanistic underpinnings of termite immunity are not well understood. Given the intimate relationship between sociality and disease, this represents a major gap in understanding, particularly since termites represent the only animal group outside of Hymenoptera to have evolved such advanced forms of social living. We explore the evolution of termite immunity by conducting qualitative and quantitative transcriptomic analyses from twelve cockroach and termite species occurring along a gradient of sociality. We incorporate lineages spanning the origin of eusociality, and include species from a key transitional subsocial group: the wood roaches. Firstly, we analyze immune characteristics in the context of termite phylogeny. Here, we examine the evolution of immune traits during the transition to eusociality, and explore the extent to which immune adaptations are associated with evolutionary shifts in social complexity and ecology. Secondly, we conduct a quantitative analysis of responses to a common immune stimulus to examine the role of both sociality and caste in immunity. Here, we test the hypothesis that the evolution of division of labour – and associated increases in investment into costly altruistic (immune) traits – occurred at the expense of individual immunity in the sterile caste. T2 - CONFLICT AND COOPERATION – BRIDGING EVOLUTION, ECOLOGY AND IMMUNOLOGY" PHD STUDENT MEETING CY - Bautzen, Germany DA - 16.03.2018 KW - Evolution KW - Termite KW - Immunity PY - 2017 AN - OPUS4-44253 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dümichen, Erik A1 - Eisentraut, Paul A1 - Braun, Ulrike T1 - Fast identification of microplastics using thermal extractions methods N2 - A new and full automated system for the analysis of microplastics in environmental samples is presented. T2 - BAM-BfR Seminar 2018 CY - Berlin, Germany DA - 15.02.2018 KW - Mikroplastik KW - TED KW - Thermal degradation PY - 2018 AN - OPUS4-44179 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braun, Ulrike T1 - Fast identification of microplastics using thermal extraction methods N2 - The presentation presents an overview about existing methods of microplastic detection with a special focus on thermo-analytical methods. T2 - Perkin Elmer Workshop Microplastics CY - Vienna, Austria DA - 11.01.2018 KW - Microplastics KW - TED-GC-MS KW - Analysis PY - 2018 AN - OPUS4-43803 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kalbe, Ute T1 - Tools for the characterization of secondary raw materials - status of leaching test application N2 - Leaching tests are used as tools for the assessment of environmental compatibility of granular waste materials with potential for reuse. The presentation gives an overview on available leaching tests, their field of application and relation to legislation. Furthermore robustness testing to elaborate optimized test conditions as well as performance characteristics from validation studies by BAM are addressed. T2 - Travelling Conference CY - Perth, Australia DA - 06.02.2018 KW - Leaching KW - Risk assessment KW - Robustness studies PY - 2018 AN - OPUS4-44723 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kalbe, Ute T1 - Tools for the characterization of secondary raw materials - status of leaching test application N2 - Leaching tests are used as tools for the assessment of environmental compatibility of granular waste materials with potential for reuse. The presentation gives an overview on available leaching tests, their field of application and relation to legislation. Furthermore robustness testing to elaborate optimized test conditions as well as performance characteristics from validation studies by BAM are addressed. T2 - Travelling Conference CY - Ho-Chi-Ming City, Vietnam DA - 01.02.2018 KW - Leaching KW - Risk assessment KW - Robustness studies PY - 2018 AN - OPUS4-44724 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Erning, Johann Wilhelm ED - Stojanovic, Ivan ED - Simunovic, Vinko ED - Runje, Biserka T1 - Release of heavy metals from alloys used in drinking water: How to test and evaluate materials N2 - Die Methodik der Prüfung und Bewertung von metallenen Werkstoffen für den Gebrauch in Trinkwasser wird beschrieben und erläutert. Die Philosophie der Zulassung wird dargestellt. T2 - Kormat 2018 CY - Zagreb, Croatia DA - 25.04.2018 KW - Metals hygienic assessment release PY - 2018 SN - 1848-4255 SP - 1 EP - 6 CY - Zagreb AN - OPUS4-44809 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Makowski, Maike A1 - Werneburg, Martina A1 - Jung, Christian A1 - Haase, H. A1 - Koch, Matthias T1 - Non-invasive Cereal Analysis by GC-MS detection of Trichodiene as a Volatile Mycotoxin Biomarker N2 - Due to the increasing consumption of cereals worldwide, the monitoring of growth, storage and processing is becoming more and more crucial. Particularly when stored, infested grains breed fungal clusters (“hot spots”) in which mycotoxins greatly exceed allowed maximum levels. Because of their unpredictable presence, current sample drawing and procedures for mycotoxin analysis represent a complex challenge for operators, involving invasive and cost intensive steps. Therefore, new time- and labour-saving mycotoxin control methods including sampling and analysis steps are needed. A possible approach is the non-invasive analysis of the homogeneous gas phase above the crops, instead of analyzing random samples. However, this procedure requires microbial volatile organic compounds (MVOC´s) being released by the samples and representing the present mycotoxins. Previous investigations revealed trichodiene to be a precursor in trichothecenes biosynthesis – one of the largest mycotoxin groups with over 180 compounds. Due to its non-functionalized sesquiterpene structure, trichodiene has already been quantified using Headspace GC-MS methods (for instance]). Thereby, it can possibly be used as a biomarker for trichothecene contamination in foodstuff. However, further investigations are necessary. The correlation between trichodiene concentration in the gas phase and trichothecenes mass fraction in the sample must be examined closely to draw conclusions about the exact trichothecene content within samples. Realizing this idea, would widely extend the applicability of trichodiene and enormously simplify trichothecene quantification. Hence, this first step of an ongoing study aims to develop a laboratory reference method using trichodiene as volatile biomarker to quantify trichothecenes in cereals. Static headspace and SPME-enrichment coupled to gas chromatography with mass spectrometry (GC-MS) were employed. In a second step, this reference method is intended to validate new approaches for fast on-site screening of trichodiene in cereals. T2 - 10th World Mycotoxin Forum Conference CY - Amsterdam, The Netherlands DA - 11.03.2018 KW - Trichodiene KW - GC-MS KW - Mycotoxin Biomarker PY - 2018 AN - OPUS4-44508 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Makowski, Maike A1 - Jung, Christian A1 - Werneburg, Martina A1 - Haase, H. A1 - Koch, Matthias T1 - Non-invasive Cereal Analysis by GC-MS detection of Trichodiene as a Volatile Mycotoxin Biomarker N2 - Due to the increasing consumption of cereals worldwide, the monitoring of growth, storage and processing is becoming more and more crucial. Particularly when stored, infested grains breed fungal clusters (“hot spots”) in which mycotoxins greatly exceed allowed maximum levels. Because of their unpredictable presence, current sample drawing and procedures for mycotoxin analysis represent a complex challenge for operators, involving invasive and cost intensive steps. Therefore, new time- and labour-saving mycotoxin control methods including sampling and analysis steps are needed. A possible approach is the non-invasive analysis of the homogeneous gas phase above the crops, instead of analyzing random samples. However, this procedure requires microbial volatile organic compounds (MVOC´s) being released by the samples and representing the present mycotoxins. Previous investigations revealed trichodiene to be a precursor in trichothecenes biosynthesis – one of the largest mycotoxin groups with over 180 compounds. Due to its non-functionalized sesquiterpene structure, trichodiene has already been quantified using Headspace GC-MS methods (for instance). Thereby, it can possibly be used as a biomarker for trichothecene contamination in foodstuff. However, further investigations are necessary. The correlation between trichodiene concentration in the gas phase and trichothecenes mass fraction in the sample must be examined closely to draw conclusions about the exact trichothecene content within samples. Realizing this idea, would widely extend the applicability of trichodiene and enormously simplify trichothecene quantification. Hence, this first step of an ongoing study aims to develop a laboratory reference method using trichodiene as volatile biomarker to quantify trichothecenes in cereals. Static headspace and SPME-enrichment coupled to gas chromatography with mass spectrometry (GC-MS) were employed. In a second step, this reference method is intended to validate new approaches for fast on-site screening of trichodiene in cereals. T2 - 10th World Mycotoxin Forum Conference CY - Amsterdam, The Netherlands DA - 11.03.2018 KW - Trichodiene KW - GC-MS KW - Mycotoxin Biomarker PY - 2018 AN - OPUS4-44509 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Sieksmeyer, Thorben A1 - McMahon, Dino Peter A1 - He, Shulin T1 - Beyond nutrition: host-microbiota interactions drive shifts in the behavioural phenotypes of cockroaches N2 - Recent studies have shown that host-microbiota interactions can lead to dramatic changes in host phenotype especially behaviour. We investigate the causal drivers of microbe-associated shifts in host phenotype by examining how feeding behaviour is modulated by a bacterial infection in an omnivorous cockroach: Blatta orientalis. We conducted food-choice experiments after challenging hosts with the common entomopathogenic soil bacterium Pseudomonas entomophila to understand the impact of bacterial pathogens on host macronutrient preference. We find that immune challenge by this bacterium drives a sharp decline in carbohydrate intake and results in a relative increase in the ratio of protein to carbohydrate consumed. Additionally, infected cockroaches reduce their overall nutrient intake. We show for the first time that cockroach feeding behaviour is dynamically modulated by a pathogen. In contrast to studies on Spodoptera moths, this modulation does not impact any of the immune parameters we measured: it does not affect the abundance of immune related proteins in the hemolymph, hemolymph antimicrobial activity, or survival. This leads to the possibility that as long-lived omnivorous species B. orientalis may be better adapted to unpredictable variation in food availability and quality. An illness-induced anorexia-like response which is thought to assist hosts in limiting nutritional resources available to pathogens is therefore the most likely cause of the observed phenotype. Reduction of carbohydrate intake by sick individuals would be consistent with such an explanation. In another cockroach species Blatella germanica we also investigate whether in addition to pathogens, host microbiota especially gut commensals are causally responsible for shifts in host behavioural phenotype and immune competence. To address this, we conduct food-choice and immunechallenge experiments in germ-free cockroaches and naïve cockroaches. T2 - XI EUROPEAN CONGRESS OF ENTOMOLOGY CY - Neapel, Italy DA - 02.07.2018 KW - Bacterial infection KW - Feeding behaviour KW - Gut microbiota KW - Illness-induced anorexia KW - Proteomics PY - 2018 AN - OPUS4-45414 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Paul, Andrea T1 - A process spectroscopic approach for the monitoring of microplastics  in environmental samples N2 - The potential of Raman and NIR process-spectroscopic as a rapid approach for the estimation of microplastics (MP) in soil matrix were tested. For this purpose, artificial MP/soil mixture samples consisting of standard soils or sand with defined ratios of MP (0,5 – 10 mass% polymer) from polyethylene (PE), polypropylene (PP), polystyrene (PS) and polyethylene terephthalate (PET) were prepared. MP particles with diameters < 2 mm and < 125 µm were obtained from industrial polymer pellets after cryo-milling. Spectra of these mixtures were collected by (i) a process FT-NIR spectrometer equipped with a reflection probe, (ii) by a cw-process Raman spectrometer and (iii) by a time-gated Raman spectrometer using fiber-optic probes. The evaluation of process-spectra was performed by chemometric methods. Whereas MP could be detected by Raman spectroscopy in coastal sand at 0.5 mass%, in standard soil detection of MP was limited to 10 – 5 mass% with the large fraction, but samples containing particles of the 125µm mass-fraction yielded no positive result at all. One reason for the lacking sensitivity could be fluorescence by soil organic matter and thus, in a next test time-gated Raman spectroscopy was applied. However, although being indeed more sensitive to the small particles this method failed at MP < 5 mass% indicating that fluorescence was not the major problem. Finally, FT-NIR was tested. Depending on the polymer, MP contents of 0,5 or 1 mass%, respectively, could be detected in standard soils and polymers identified. Furthermore, this approach could be used for the investigation of mixtures of up to four polymers and in real-world samples from bio-waste fermenter residues. T2 - Einladung zu einem Beratungsgespräch zum Thema Mikroplastik durch die Firma Endress+Hauser Conducta GmbH+Co.KG CY - Gerlingen, Germany DA - 11.06.2018 KW - Chemometrics KW - Microplastics KW - Raman KW - Process-spectroscopy KW - NIR PY - 2018 AN - OPUS4-45406 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Makowski, Maike A1 - Werneburg, M. A1 - Jung, Christian A1 - Haase, H. A1 - Koch, Matthias T1 - Non-invasive GC-MS Analysis of Trichodiene as Volatile Mycotoxin Biomarker in Cereals N2 - Due to the increasing consumption of cereals worldwide, monitoring of growth, storage and processing is becoming more crucial. Particularly when stored, infested grains breed fungal clusters (“hot spots”) in which mycotoxins greatly exceed allowed maximum levels. Because of their unpredictable presence, current sampling and procedures for mycotoxin analysis are complex and contain invasive and cost intensive steps. A possible approach to avoid sampling inaccuracies is the non-invasive analysis of the homogeneous gas phase above the crops, instead of analyzing random samples. However, this procedure requires microbial volatile organic compounds (MVOC´s) being released by the samples. Previous investigations revealed trichodiene to be a precursor in trichothecenes biosynthesis – one of the largest mycotoxin groups with more than 180 compounds. Due to its non-functionalized sesquiterpene structure, quantification of trichodiene using Headspace GC-MS methods is possible (e.g. [1]). Thereby, it could be used as biomarker for trichothecene contamination in foodstuff. However, further studies are necessary. The correlation between trichodiene concentration in the gas phase and trichothecenes mass fraction in the sample must be examined closely to draw conclusions about the trichothecene content in samples. Realizing this idea, would widely extend the applicability of trichodiene and enormously simplify trichothecene quantification. Hence, this first step of an ongoing study aims to develop a laboratory reference method using trichodiene as volatile biomarker to quantify trichothecenes in cereals. Static headspace and SPME-enrichment coupled to gas chromatography with mass spectrometry (GC-MS) were employed. In a second step, this reference method is intended to validate new approaches for fast on-site screening of trichodiene in cereals. T2 - 40th Mycotoxin Workshop CY - Munich, Germany DA - 11.06.2018 KW - Trichodiene KW - Gaschromatography-Mass Spectrometry KW - Mycotoxin Biomarker PY - 2018 AN - OPUS4-45314 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braun, Ulrike A1 - Klöckner, P. A1 - Wagner, S. A1 - Ruhl, A.S. A1 - Eisentraut, P. A1 - Albrecht, M. A1 - Reemtsma, T. T1 - Determination of tire wear particles based on elemental composition N2 - In this presentation the use of ICP-MS for the analysis of tire wear particles in environmental samples is presented. T2 - Wassertagung der GdCh CY - Papenburg, Germany DA - 07.05.2018 KW - ICP-MS KW - Mikroplastik KW - Analytik KW - Reifenabrieb PY - 2018 AN - OPUS4-45201 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braun, Ulrike A1 - Klöckner, P. A1 - Wagner, S. A1 - Ruhl, A.S. A1 - Eisentraut, Paul A1 - Albrecht, M. A1 - Reemtsma, T. T1 - Identification of tire wear particles based on elemental composition N2 - The identification of tire wear particles by use of ICP-MS is presented. T2 - SETAC CY - Rome, Italy DA - 13.05.2018 KW - Microplastics KW - Tire wear particles KW - ICP-MS PY - 2018 AN - OPUS4-45205 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - C., Ruibal A1 - L., Selbmann A1 - Serap, Avci A1 - Martin-Sanchez, Pedro Maria A1 - Gorbushina, Anna ED - Gorbushina, Anna T1 - Roof-Inhabiting Cousins of Rock-Inhabiting Fungi: Novel Melanized Microcolonial Fungal Species from Photocatalytically Reactive Subaerial Surfaces N2 - Subaerial biofilms (SAB) are an important factor in weathering, biofouling, and biodeterioration of bare rocks, building materials, and solar panel surfaces. The realm of SAB is continually widened by modern materials, and the settlers on these exposed solid surfaces always include melanized, stress-tolerant microcolonial ascomycetes. After their first discovery on desert rock surfaces, these melanized chaetothyrialean and dothidealean ascomycetes have been found on Mediterranean monuments after biocidal treatments, Antarctic rocks and solar panels. New man-made modifications of surfaces (e.g., treatment with biocides or photocatalytically active layers) accommodate the exceptional stress-tolerance of microcolonial fungi and thus further select for this well-protected ecological group. Melanized fungal strains were isolated from a microbial community that developed on highly photocatalytic roof tiles after a long-term environmental exposure in a maritime-influenced region in northwestern Germany. Four of the isolated strains are described here as a novel species, Constantinomyces oldenburgensis, based on multilocus ITS, LSU, RPB2 gene phylogeny. Their closest relative is a still-unnamed rock-inhabiting strain TRN431, here described as C. patonensis. Both species cluster in Capnodiales, among typical melanized microcolonial rock fungi from different stress habitats, including Antarctica. These novel strains flourish in hostile conditions of highly oxidizing material surfaces, and shall be used in reference procedures in material testing. KW - Microcolonial fungi KW - Multilocus phylogeny KW - Photocatalytic surfaces KW - Subaerial biofilms KW - Stress tolerance KW - Constantinomyces PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-455182 DO - https://doi.org/10.3390/life8030030 VL - 8 IS - 3 SP - 30 EP - 44 PB - MDPI CY - Basel, Schweiz AN - OPUS4-45518 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braun, Ulrike A1 - Bannick, C. G. T1 - Sampling, sample preparation and detection of microplastics, current activities in the ISO/TC 61/SC14/WG 4 N2 - Presentation about the actual status of microplastic research in BAM and UBA, activities in Germany and ISO/TC61/SC14. T2 - Microplastic Methodes Workshop CY - London, UK DA - 23.05.2018 KW - Microplastics KW - TED-GC-MS KW - Analysis KW - Standardisation PY - 2018 AN - OPUS4-45033 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dittmann, Daniel A1 - Braun, Ulrike A1 - Jekel, M. A1 - Ruhl, A. S. T1 - Quantification and characterisation of activated carbon in activated sludge by thermogravimetric and evolved gas analyses N2 - Advanced wastewater treatment with powdered activated carbon (PAC) leads to a spread of PAC into different purification stages of wastewater treatment plants (WWTP) due to recirculation and filter back-wash. Currently, no methods for quantification of PAC in activated sludge are available. In this study, PAC containing activated sludge from four WWTP were examined by two-step thermogravimetric analysis (TGA) with heating up to 600°C in N2 and subsequently in synthetic air. Direct quantification of PAC according to temperature specific weight losses was possible for one WWTP. Quantification by combining specific mass losses was found to be an alternative direct method, with a detection limit of 1.2% PAC in dry sample mass. Additionally, evolved gas analysis (EGA) by infrared-spectroscopy (FTIR) during TGA revealed interaction mechanisms between PAC and activated sludge. Aliphatic compounds from activated sludge were identified as major substances influenced by PAC. In derivative thermogravimetry (DTG), a typical double peak at approximately 300°C was found to be related to carbonylic species with increased evolution of acetic acid in aged activated sludge. TGA and EGA are promising tools to understand, control and optimise the application of PAC in advanced wastewater treatment. KW - Advanced wastewater treatment KW - Powdered activated carbon KW - Sewage treatment plant KW - Thermoanalysis KW - Thermogravimetry KW - Fourier transform infrared spectroscopy PY - 2018 UR - http://www.sciencedirect.com/science/article/pii/S2213343718301313 DO - https://doi.org/10.1016/j.jece.2018.03.010 SN - 2213-3437 VL - 6 IS - 2 SP - 2222 EP - 2231 PB - Elsevier CY - Amsterdam AN - OPUS4-44978 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Oberpaul, M. A1 - Spohn, M. A1 - Fracowiak, J. A1 - Plarre, Rüdiger A1 - McMahon, Dino Peter A1 - Hammann, P. A1 - Vilcinskas, A. A1 - Gläser, J. T1 - Exploiting termite nest microbiomes for antibiotic discovery by using an ultra-high throughput Microfluidics/FACS driven pipeline combined with a microtiter plate based cultivation strategy N2 - Infections with multi-resistant Gram negative pathogens are a major threat to our health system. In order to serve the needs in antibiotics development we selected untapped bioresources and implemented high throughput approaches suitable for the discovery of strains producing antibiotics with anti-Gram negative activity. Our approaches rely on the hypothesis that Termite associated bacteria are likely to produce potent antibiotics to defend their hosts against entomopathogenic microorganisms. Termite nests and guts harbor suitable, highly diverse microbiomes in which bacterial taxa are present known to potentially produce natural compounds. In a first step the diversity of Coptotermes species nest microbiomes was assessed carefully by using 16S rDNA amplicon sequencing on the Illumina MiSeq platform and nest material was selected to retrieve viable cells by using Nycodenz density gradient centrifugation. In order to analyze the diversity of the culturable termite nest microbiome, bacterial cells were either distributed in 384-well plates (approach 1) or encapsulated in small spheric agarose beads by an high throughput microfluidics technique (approach 2). Cultures obtained from approach 1 were scaled-up in 96-well Duetz-systems for characterization of diversity and for rapid supernatant screening using the bioluminescence-labeled E. coli pFU166. The generated droplets of approach 2 simultaneously received a small population of GFP-tagged Gram negative screening cells and were sorted for low fluorescence using FACS. After elimination of redundancy we performed a fast scale-up of active strains. Implementation of this pipeline allows us to prioritize antibiotics producing strains in a ultra-high throughput fashion and by cultivation of broad diversity in our approches. T2 - Annual Conference of the Society for General and Applied Microbiology (VAAM) CY - Wolfsburg, Germany DA - 15.04.18 KW - Biotechnology KW - Termites KW - Anti-microbial effects PY - 2018 AN - OPUS4-44987 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mueller, Axel A1 - Duemichen, E. A1 - Eisentraut, Paul A1 - Braun, Ulrike A1 - Scholz, K. A1 - Bannick, C.-G. T1 - Analysing microplastics in samples of terrestrial systems N2 - The presence, fate and effects of microplastics (MP) in terrestrial systems are largely unknown. The few existing studies investigated either agricultural or industrial sites. Several techniques were used for analysis, primarly spectroscopic methods such as FTIR or Raman. Sample pretreatments like density separations are common to reduce matrix. A lack of harmonised and standardised sampling instructions for microplastic investigations in the terrestrial area was identified as particular critical, because different studies are barely comparable. The aim of the project is to develop a proposal for a harmonized procedure for sampling, sample preparation and the detection of microplastics in terrestrial matrices for total content determination. By detecting specific degradation products the thermal extraction desorption gas chromatography mass spectrometry (TED-GC-MS) allows a direct determination of mass content of MP in environmental samples. T2 - SETAC 2018 CY - Rome, Italy DA - 13.05.2018 KW - Microplastics KW - Soil sample KW - TED-GC-MS KW - Analysis PY - 2018 AN - OPUS4-44988 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bandow, Nicole A1 - Jürgens, Frederike A1 - Schoknecht, Ute T1 - Leachiing tests - a useful tool for the environmental impact assessment of construction products? N2 - Construction products and waste materials used for construction can be in contact with the environment and may release potential harmful compounds. Information on the total content of these substances in the product is not sufficient to assess its envrionmental impact since it does not consider realistic exposure conditions.The impact of these compounds is often assessed by using the total content in the product. This estimation lacks realistic exposure conditions. Concerning the pathway to soil and groundwater by contact with rain or seepage water leaching tests, which were developed and standardized by the European Committee for Standardization, are available. While for secondary construction products and waste this investigation of the leaching behaviour is standard procedure and is already part of regulation in Germany (draft ordinance on reuse of mineral waste) and within Europe (landfill directive) the release from primary construction products got in the focus more recently. Several monitoring studies found unexpected high concentrations of substances used as herbicides and fungicides in surface water and stormwater originating from urban areas. As some of these compounds are even banned for the use in agricultural applications in Europe alternative sources as roof materials and façade coatings exposed to rain were suggested. Further field and laboratory tests confirmed construction products as sources of these substances in water. The aim of this presentation is to show exemplary results of existing leaching methods and underline the strength and weaknesses of the test system with selected examples from our work. We especially draw the attention to the research which is still needed to close the gap between the results of leaching experiments and the subsequent risk assessment of the products. T2 - SETAC Europe CY - Rome, Italy DA - 13.05.2018 KW - Leaching KW - DSLT KW - Construction products PY - 2018 AN - OPUS4-44949 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bandow, Nicole T1 - Consideration of organic contaminants and ecotoxicologal impact of recycling materials - Handling of substances without treshold values N2 - The presentation gives an overview about the use of ecotoxicity testing in assessment of waste. Especially in cases with unknown composition this kind of test are useful. Recently, the assessment of the hazard property HP 14 "ecotoxicity" for the labelling of waste was published. The labelling procedure is explained in detail. T2 - Travelling Conference CY - Perth, Australia DA - 06.02.2018 KW - Ecotoxicity KW - HP14 KW - Recycling materials PY - 2018 AN - OPUS4-44456 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bandow, Nicole T1 - Consideration of organic contaminants and ecotoxicologal impact of recycling materials - Handling of substances without treshold values N2 - The presentation gives an overview about the use of ecotoxicity testing in assessment of waste. Especially in cases with unknown composition this kind of test are useful. Recently, the assessment of the hazard property HP 14 "ecotoxicity" for the labelling of waste was published. The labelling procedure is explained in detail. T2 - Travelling Conference CY - Seoul, Korea DA - 29.01.2018 KW - Ecotoxicity KW - Recycling materials KW - HP14 PY - 2018 AN - OPUS4-44454 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bandow, Nicole T1 - Consideration of organic contaminants and ecotoxicologal impact of recycling materials - Handling of substances without treshold values N2 - The presentation gives an overview about the use of ecotoxicity testing in assessment of waste. Especially in cases with unknown composition this kind of test are useful. Recently, the assessment of the hazard property HP 14 "ecotoxicity" for the labelling of waste was published. The labelling procedure is explained in detail. T2 - Travelling Conference CY - Ho-Chi-Ming City, Vietnam DA - 01.02.2018 KW - Ecotoxicity KW - HP14 KW - Recycling materials PY - 2018 AN - OPUS4-44455 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hofmann, Michael A1 - Moufid, M. A1 - El Bari, N. A1 - Tiebe, Carlo A1 - Bouchikhi, B. T1 - Multigas Sensor System for Outdoor Odour Nuisance Monitoring N2 - The presentation gives a brief overview of the main goals, the used methods and the first results of the PMARS OdourSense project. In cooperation with the Moulay Ismaïl University in Meknès (Morocco), a multi-gas sensor system for odour nuisance monitoring in outdoor air and a method for testing/calibration of such devices are being developed in this project. T2 - German-Moroccan Workshop on PMARS III CY - Bonn, Germany DA - 20.06.2018 KW - Air-pollution monitoring KW - Multi-gas sensor system KW - Odour KW - Electronic nose KW - Outdoor odour control PY - 2018 AN - OPUS4-45266 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lauer, Franziska A1 - Diehn, Sabrina A1 - Seifert, Stephan A1 - Kneipp, Janina A1 - Sauerland, V. A1 - Barahona, C. A1 - Weidner, Steffen T1 - Multivariate analysis of MALDI imaging mass spectrometry data of mixtures of single pollen grains N2 - Here, we present an advanced approach to identify pollen grains in mixtures based on: - a simplified sample preparation procedure - MALDI imaging mass spectrometry - chemometric tools Our goals are to: - explore the biomolecular variations in pollen - determine species-specific peak patterns - discriminate and identify single pollen grains in mixtures using MSI T2 - 66th Conference on Mass Spectrometry and Allied Topics CY - San Diego, CA, USA DA - 03.06.2018 KW - Pollen PY - 2018 AN - OPUS4-45196 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Isleyen, A. A1 - Vogl, Jochen A1 - Nikolic, D. A1 - Jotanovic, A. A1 - Näykki, T. A1 - Perkola, N. A1 - Horvat, M. A1 - Zon, A. A1 - Bulska, E. A1 - Ochsenkühn-Petropoulou, M. A1 - Can, S. Z. A1 - Jacimovic, R. A1 - Gazevic, L. A1 - Cankur, O. A1 - Ari, B. A1 - Tunc, M. A1 - Binici, B. A1 - Gokcen, T. A1 - Cakilbahce, Z. A1 - Lymperopoulou, V. T1 - Three candidate certified reference materials for environmental analysis N2 - This project aims to develop capacity to produce certified reference materials (CRMs) for environmental analysis by transferring know-how between the partners and combining their skills to focus on environmental CRM production. The production process includes good manufacturing practices for processing materials, method development, the validation and application of homogeneity, stability and characterisation tests, the calculation of individual uncertainties (between-unit inhomogeneity, long term stability, characterisation) and combination of uncertainties to determine overall uncertainty of the matrix reference materials. An inter laboratory comparison registered as a EURAMET project is set as the ultimate project outcome, confirming the partners’ capabilities in applying newly acquired skills. T2 - 10th International Conference on Instrumental Methods of Analysis CY - Heraklion, Greece DA - 17.09.2017 KW - Certified reference materials KW - Toxic metals KW - PFOS KW - PFOA PY - 2017 AN - OPUS4-45253 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Näykki, Teemu A1 - Perkola, N. A1 - Isleyen, A. A1 - Vogl, Jochen A1 - Skundrić, N. A1 - Jotanović, A. A1 - Horvat, M. A1 - Zoń, A. A1 - Bulska, E. A1 - Ochsenkuhn-Petropoulou, M. A1 - Can, S. Z. A1 - Bilsel, M. A1 - Hafner, K. A1 - Suljagić, Š. A1 - Kovačević, L. A1 - Jaćimović, R. A1 - Gažević, L. A1 - Arı, B. A1 - Tunc, M. A1 - Binici, B. A1 - Gökcen, T. A1 - Fotis, T. T1 - A joint research project for the sustainable production of certified matrix reference materials for environmental analysis N2 - This project aims to develop capacity to produce certified reference materials (CRMs) for environmental analysis by transferring know-how between the partners and combining their skills to focus on environmental CRM production. The production process includes good manufacturing practices for processing materials, method development, the validation and application of homogeneity, stability and characterisation tests, the calculation of individual uncertainties (between-unit inhomogeneity, long term stability, characterisation) and combination of uncertainties to determine overall uncertainty of the matrix reference materials. An inter laboratory comparison registered as a EURAMET project is set as the ultimate project outcome, confirming the partners’ capabilities in applying newly acquired skills. T2 - EcoBalt 2016 CY - Tartu, Estonia DA - 09.10.2016 KW - Certified reference material KW - Toxic metals KW - PFOS KW - PFOA PY - 2016 AN - OPUS4-45254 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Isleyen, A. A1 - Vogl, Jochen A1 - Nikolic, D. A1 - Jotanovic, A. A1 - Näykki, T. A1 - Perkola, N. A1 - Horvat, M. A1 - Zoń, A. A1 - Bulska, E. A1 - Ochsenkuhn-Petropoulou, M. A1 - Can, S. Z. A1 - Bilsel, M. A1 - Hafner, K. A1 - Jacimovic, R. A1 - Gažević, L. T1 - Joint research project for the production of certified matrix reference materials for environmental analysis N2 - Reliable analysis of chemical indicators in water, sediment and soil samples for the purpose of environmental pollution assessment poses one of the greatest analytical challenges, having in mind the complexity of sample matrix and low concentrations of pollutants. Organics (pesticides, PAHs, PFOS, etc.) and heavy metals (Hg, Cd, Ni, Pb and As) represent target parameters. Laboratories performing sampling and tests in this field regulated by respective EU directives, need strong support in order to establish a quality system. It is necessary to provide appropriate calibrators i.e. matrix CRMs relates to the unique sample matrices representing typical samples in the geomorphological and anthropological sense. In addition to that, bearing in mind the complexity and instability of environmental samples, it is very difficult to obtain appropriate referents materials with no local providers. Our project is aiming to develop capacity to produce CRMs for environmental analysis by transferring the theoretical and practical know-how between the partners and combining their skills to focus on environmental CRM production in accordance with ISO Guide 34. Our project will have an impact on environmental monitoring in the partnering countries and on the scientific community, who will use the newly developed reference materials. Furthermore, partners will develop strategies for producing new CRMs either on their own or in cooperation. This will lead to regional CRM producers serving scientific and official laboratories. T2 - 2nd International Congress of Chemists and Chemical Engineers of B&H CY - Sarajevo, Bosnia and Herzegovina DA - 21.10.2016 KW - CRM KW - Environmental analysis KW - CRM producer KW - Quality system PY - 2016 AN - OPUS4-45257 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Neumann, Patrick P. A1 - Tiebe, Carlo A1 - Bartholmai, Matthias A1 - Hüllmann, Dino A1 - Krentel, Daniel A1 - Kluge, Martin A1 - Kohlhoff, Harald A1 - Lilienthal, A.J. T1 - Airborne Remote Gas Sensing and Mapping N2 - Leaking methane (CH4) from infrastructures, such as pipelines and landfills, is critical for the environment but can also pose a safety risk. To enable a fast detection and localization of these kind of leaks, we developed a novel robotic platform for aerial remote gas sensing. Spectroscopic measurement methods for remote sensing of selected gases lend themselves for use on mini-copters, which offer a number of advantages for inspection and surveillance over traditional methods. No direct contact with the target gas is needed and thus the influence of the aerial platform on the measured gas plume can be kept to a minimum. This allows to overcome one of the major issues with gas-sensitive mini-copters. On the other hand, remote gas sensors, most prominently Tunable Diode Laser Absorption Spectroscopy (TDLAS) sensors have been too bulky given the payload and energy restrictions of mini-copters. Here, we present the Unmanned Aerial Vehicle for Remote Gas Sensing (UAV-REGAS), which combines a novel lightweight TDLAS sensor with a 3-axis aerial stabilization gimbal for aiming on a versatile hexacopter. The proposed system can be deployed in scenarios that cannot be addressed by currently available robots and thus constitutes a significant step forward for the field of Mobile Robot Olfaction (MRO). It enables tomographic reconstruction of gas plumes and a localization of gas sources. We also present first results showing its performance under realistic conditions. T2 - Networked Environmental Monitoring – from sensor principles to novel services CY - BAM, Berlin, Germany DA - 06.02.2018 KW - Localization of gas sources KW - Mobile Robot Olfaction KW - Tomographic reconstruction of gas plumes KW - Tunable Diode Laser Absorption Spectroscopy (TDLAS) KW - UAV-REGAS PY - 2018 AN - OPUS4-44085 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Neumann, Patrick P. A1 - Hüllmann, Dino A1 - Kohlhoff, Harald A1 - Bartholmai, Matthias A1 - Bennetts, V.H. A1 - Lilienthal, A.J. T1 - Mobile robot olfaction with flying platforms N2 - This presentation gives an introduction to the gas-sensitive aerial robots developed at BAM, including various application examples in the field of mobile robot olfaction: gas source localization and gas distribution mapping T2 - Networked Environmental Monitoring – from sensor principles to novel services CY - BAM, Berlin, Germany DA - 06.02.2018 KW - Localization of gas sources KW - Mobile Robot Olfaction KW - Tomographic reconstruction of gas plumes KW - Tunable Diode Laser Absorption Spectroscopy (TDLAS) KW - UAV-REGAS PY - 2018 AN - OPUS4-44086 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bartholmai, Matthias A1 - Schütze, A. T1 - Sensor network deployment N2 - Content • Fixed monitoring stations • Mobile monitoring stations (on trams, buses, cars) • Personal mobile monitoring systems • Environmental monitoring for agriculture and beyond • Sensors on flying platforms T2 - Networked Environmental Monitoring – from sensor principles to novel services CY - BAM, Berlin, Germany DA - 06.02.2018 KW - Environmental monitoring KW - Gas sensors PY - 2018 AN - OPUS4-44073 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bartholmai, Matthias A1 - Neumann, Patrick P. A1 - Tiebe, Carlo A1 - Johann, Sergej T1 - Adaptable multi-sensor device for gas detection N2 - Innovation is the catalyst for the technology of the future. It is important to develop new and better technologies that can continuously monitor the environmental impact, e.g., for air Quality control or emission detection. In the recently at BAM developed Universal Pump Sensor Control (UPSC3) module, different components and sensors are fused. The combination of the individual components makes the UPSC3 module an excellent monitoring and reference system for the development and characterization of gas specific sensors. Measurements over long periods are possible, for mixed gas loads or for certain gas measurements. The System is part of a mobile sensor network of several sensor units, which can also be used as standalone systems. T2 - Networked Environmental Monitoring – from sensor principles to novel services CY - BAM, Berlin, Germany DA - 06.02.2018 KW - Environmental monitoring PY - 2018 AN - OPUS4-44077 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rübner, Katrin A1 - Hempel, S. A1 - Lindemann, Mathias A1 - Peplinski, Burkhard A1 - Vogler, Nico A1 - Zimathies, Annett T1 - Comparative study of lightweight aggregates N2 - Lightweight aggregates (LWA) are mixtures of porous grains of mineral origin characterised by particle bulk densities less than 2000 kg/m³ or loose bulk densities less than 1200 g/m³. They are used in concrete, mortar and grout in buildings, roads and civil engineering works. LWA are commonly produced by thermal treatment of natural raw materials such as clay, shale and other clayey or igneous rocks at temperatures between 1000 °C and 1300 °C. Expanded clay, expanded shale, vermiculite and expanded perlite are well-known aggregates used in the construction industry today. But porous LWA can also be manufactured from mineral residues, such as waste glass or masonry rubble, by thermal expending process. The source materials must satisfy two conditions. First, their chemical composition must be in balance between fluxing agents, silica and alumina to reach pyroplastic conditions at an economically viable temperature to develop sintering and melting phases of proper viscosity. Second, the material should contain some components, which ensure gas release during the partial melting, to form expanded porous particles. Lightweight aggregates usually show a heterogeneous and macroporous pore structure. Their solid skeleton is continually opened by large bulky pores whereas the skeletal walls contain many small pores. Additionally, the grains consist of partly melted areas. The specific microstructural and morphological characteristics of different LWA are strongly influenced by the great variety of source materials and the particular manufacturing conditions. Recycling granules (RC granules), which are lightweight aggregates made from masonry rubble containing about 50 % brick material, are already described in terms of manufacturing process, engineering properties and microstructure in. The objective of this study is to compare the chemical, physical, mineralogical and microstructural characteristics of RC granules with those of commercially produced expanded clay aggregates. Results of the ICP optical emission spectrometry, X-ray diffraction, thermal analysis, fusibility tests, particle shape analysis, porosity measurements, mercury intrusion porosimetry und environmental scanning electron microscopy are presented. The microstructural characteristics are correlated with engineering properties of the lightweight aggregates, such as water absorption and particle strength. The study was part of two research projects that were supported by the Federal Ministry of Education and Research in the framework of the funding initiative “r2-Innovative Technologies for Resource Efficiency - Resource-Intensive Production Processes” and the Bundesministerium für Wirtschaft und Energie in the framework of the Central Innovation Programme for SMEs. T2 - 11th International Symposium on the Charcterization of Porous Solids (COPS-XI) CY - Avignon, France DA - 14.05.2017 KW - Lightweight aggregates (LWA) KW - Composition and pore structure KW - Recycling granules KW - Expanded clay PY - 2017 AN - OPUS4-44120 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lauer, Franziska A1 - Diehn, Sabrina A1 - Weidner, Steffen A1 - Kneipp, Janina T1 - A graphical user interface for a fast multivariate classification of MALDI-TOF MS data of pollen grains N2 - The common characterization and identification of pollen is a time-consuming task that mainly relies on microscopic determination of the genus-specific pollen morphology. A variety of spectroscopic and spectrometric approaches have been proposed to develop a fast and reliable pollen identification using specific molecular information. Amongst them, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows a high potential for the successful investigation of such complex biological samples. Based on optimized MALDI sample preparation using conductive carbon tape, the application of multivariate statistics (e.g. principal components analysis, PCA) yields an enormous improvement concerning taxonomic classification of pollen species compared to common microscopic techniques. Since multivariate evaluation of the recorded mass spectra is of vital importance for classification, it’s helpful to implement the applied sequence of standard Matlab functions into a graphical user interface (GUI). In this presentation, a stand-alone application (GUI) is shown, which provides multiple functions to perform fast multivariate analysis on multiple datasets. The use of a GUI enables a first overview on the measured dataset, conducts spectral pretreatment and can give classification information based on HCA and PCA evaluation. Moreover, it can be used to improve fast spectral classification and supports the development of a simple routine method to identify pollen based on mass spectrometry. T2 - 12. Interdisziplinäres Doktorandenseminar, GDCh AK Prozessanalytik CY - BAM, AH, Berlin, Germany DA - 25.03.2018 KW - MALDI KW - GUI KW - Pollen PY - 2018 AN - OPUS4-44661 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Taube, Mareike Carolin A1 - Adam, Christian A1 - Adamczyk, Burkart A1 - Schulenburg, F. A1 - Bartmann, U. A1 - Beckmann, T. A1 - Michalik, K. A1 - Reuter, M. A1 - Stelter, M. T1 - Optimisation of a Pyrometallurgical Niobium and Tantalum Recyling Process with on-line-LIBS N2 - Tantalum and niobium are essential for the development of electronics towards a more and more compact design, but without reducing their performance today. Especially in smart phones and tablets, tantalum and niobium capacitors with high charge densities have already become almost indispensable. However, tantalum as a critical raw material is still a problem for the sustainable production of electronics. Due to this fact, the existence of efficient recycling processes especially in Europe is becoming even more important nowadays. The Bundesanstalt für Materialforschung und -prüfung (BAM) is currently working on the optimisation of an existing pyrometallurgical tantalum recycling process. Optimisation of this industrial process is carried out in a small-scale electric arc furnace (480 kVA, capacity approx. 150 kg/h) at BAM using a new and innovative equipment for on-line analysis of high temperature processes. The aim of this project is to identify the best timing for an optimum slag tapping, when the slag is lower than minimum targeted tantalum concentration. Hence, LIBS (Laser induced breakdown spectroscopy) is used to identify the chemical composition of the slag layer and during slag tapping. The on-line-LIBS prototype of BAM enables an in-situ measurement of the element distribution in the melt after calibration on the slag system. First results of this joint research project will be presented including on-line-LIBS-measurements and thermodynamic and kinetic aspects of the process. T2 - European Mineral Processing & Recycling Congress (EMPRC 2018) CY - Essen, Germany DA - 25.06.2018 KW - Laser-induced breakdown spectroscopy KW - Process control KW - Tantalum KW - In-situ analysis KW - Pyrometallurgy PY - 2018 AN - OPUS4-45614 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kotthoff, Lisa A1 - Schwerdtle, T. A1 - Koch, Matthias T1 - Investigation of ionophore antibiotics and their transformation products by using electrochemistry coupled to LC-MS N2 - Ionophore antibiotics are used to cure and prevent coccidiosis by chicken especially in broiler farming. The residues are found not only in food products (chicken and eggs) but also in the environment (manure, soil or water). In this work the ionophores monensin (MON), salinomycin (SAL), maduramicin (MAD) and lasalocid (LAS) are investigated aiming to study their transformation products (TPs) through biotransformation processes. Biotransformation can be divided into two phases, phase I: oxidation, reduction or hydrolysis and Phase II: conjugation reactions. It is necessary to further examine the biotransformation pathways to determine TPs to be able to detect residues more specifically in different matrices. The technique of electrochemistry (EC) offers the opportunity to simulate biotransformation processes and to generate TPs for further analysis. The combination of EC with liquid chromatography and mass spectrometry (EC-LC-MS) provide a fast and simple tool to separate and determine the EC-generated TPs. The electrochemical flow through cell is coupled to the (LC)-MS system, allowing the reaction mixture to be separated by a RP-18 column and then analyzed in the MS. The oxidation products are generated at different potentials between 0.0 – 2.5 V vs. Pd/H2 using glassy carbon or boron doped diamond as working electrode materials . The results show a broad spectrum of different TPs depending on used solvents and working electrode materials. Among the generated TPs already known as well as unknown TPs of the drugs can be found. Further investigations on structure elucidation of unkown TPs are planned. T2 - World Conference on Analytical and Bioanalytical Chemistry CY - Barcelona, Spain DA - 23.07.2018 KW - Transformation Product KW - Electrochemistry KW - Ionophore Antibiotics PY - 2018 AN - OPUS4-45600 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mansurova, Maria A1 - Bell, Jérémy A1 - Gotor, Raul A1 - Rurack, Knut T1 - Fluorescence-based optical system for the detection of total petroleum hydrocarbons in water and soil with smartphone read-out compatibility - Spectrocube, a sensor for rapid oil test in water and soil N2 - Contamination of natural bodies of water with oil and lubricants (or generally, hydrocarbon derivatives such as petrol, fuel and others) is a commonly found phenomenon around the world due to the extensive production, transfer and use of fossil fuels. The timely identification of these contaminants is of utmost importance, since they directly affect water quality and represent a risk for wildlife and human health even in trace amounts. In this work, we develop a simple system for the on-field detection of total petroleum hydrocarbons (TPH) in water and soil, the "Spectrocube". The test is based on the measurement of the fluorescence signal emitted by the molecular rotor 4-DNS-OH dye. This dye is embedded in a hydrophobic polymeric matrix (PVDF), avoiding interactions of water with the dye and providing a robust support for use in test-strip fashion. The test-strip’s fluorescence intensity increases linearly at low concentrations of TPH, reaching a saturation value at higher concentrations. For excitation and evaluation of the test-strip fluorescence, a simple miniature optical system was designed. The system works semi-quantitatively as solvent-free TPH detection kit, as well as quantitatively when using a simple cyclopentane extraction step. To simplify the fluorescence read-out, the device is coupled to a tablet computer via Bluetooth, running a self-programmed software ("app"). T2 - Oil Spill India 2018 CY - New Delhi, India DA - 05.07.2018 KW - Oil analysis KW - Water analysis KW - Fluorescence KW - Spectrocube KW - Rapid test KW - Field test KW - Spectroscopy KW - Sensor PY - 2018 AN - OPUS4-45606 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Keller, Julia A1 - Borzekowski, Antje A1 - Haase, H. A1 - Rueß, L. A1 - Menzel, R. A1 - Koch, Matthias T1 - Using the model organism Caenorhabditis elegans for the toxicity testing of citrinin, zearalenone and zearalenone-14-sulfate N2 - To keep up with emerging mycotoxins and their transformation products fast and reliable toxicity tests are needed. Toxicity testing of mycotoxins is carried out usually by performing in vitro assays or is evaluated by using laboratory animals like mice, rats or chicken in in vivo studies. Settled between classical in vitro approaches and in vivo studies with higher animals are tests with the nematode Caenorhabditis elegans. Since Sydney Brenner described 1974 the cultivation and handling of C. elegans, this worm is widely used as model organism in developmental biology and neurology. Due to many benefits like easy and cheap cultivation, a completely sequenced genome and short generation time, it also plays an important role in toxicological research. Finally, the high number of conserved genes between human and C. elegans make the worm an ideal candidate for toxicological investigations. In this study we used C. elegans to assess the toxic effects of the relevant food mycotoxin citrinin (CIT), the mycoestrogen zearalenone (ZEN) and the modified mycotoxin ZEN-14-sulfate (ZEN-14-S) on different lifetable parameters including reproduction, thermal and oxidative stress tolerance and lifespan. All tested mycotoxins significantly decreased the amount of offspring. In case of ZEN and CIT also significant negative effects on stress tolerance and lifespan were observed compared to the control group. Moreover, metabolization of mycotoxins in the worms was investigated by using LC MS/MS. Extraction of the worms treated 5 days with mycotoxin-containing and UVC-killed bacteria showed metabolization of ZEN to α-ZEL and β-ZEL (ZEL = zearalenol, ratio about 3:2). ZEN 14-S was reduced to ZEL 14-S and CIT was metabolized to mono hydroxylated CIT. T2 - 40th Mycotoxin Workshop CY - Munich, Germany DA - 11.06.2018 KW - Mycotoxins KW - Caenorhabditis elegans KW - Toxicity testing PY - 2018 AN - OPUS4-45170 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koerdt, Andrea A1 - An, Biwen A1 - Kleinbub, Sherin T1 - Microbial corrosion coupled to methanogenesis by strains from different environments N2 - Microbiologically influenced corrosion (MIC) of iron is usually attributed to sulfate-reducing microorganisms, either chemically (formation of hydrogen sulfide) or electrically (direct electron uptake). Methanogenetic Archaea are also known to be involved in iron corrosion, forming a multi-species biofilm on corroding metallic structures. However, mechanistic details and kinetics of the overall process in methanogen-induced MIC are poorly understood. T2 - Beirat Material CY - Berlin, Germany DA - 26.04.2018 KW - Corrosion KW - Methanogens KW - Archaea KW - Implants KW - Biofilm PY - 2018 AN - OPUS4-45112 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wöhlecke, Andreas T1 - Agency perspectives on geomembrane durability, service life and end of life N2 - Die Präsentation erläutert die Hintergründe der nationalen Anforderungen für die Verwendung von Kunststoffdichtungbahnen in Deponieabdichtungen. Die Beständigkeit wird diskutiert. Anforderungen an das Qualitatsmanagement während der Produktion der Produkte und der Bauphase werden aufgezeigt und die Auswirkungen auf die langzeitige Funktionserfüllung werden beschrieben. T2 - IGS TC-B GeoBarrier Workshop CY - Munich, Germany DA - 06.06.2018 KW - Beständigkeit KW - Kunststoffdichtungsbahnen KW - Deponiebau PY - 2018 AN - OPUS4-45123 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bresch, Harald A1 - Schmidt, Alexandra A1 - Bachmann, V. A1 - Kämpf, K. A1 - Kuhlbusch, T. A1 - Schwirn, K. A1 - Völker, D. T1 - OECD Test Guideline on particle size and size distribution of manufactured nanomaterials N2 - The properties of nanomaterials are influenced not only by their chemical composition but also by physical properties (such as size, geometry and crystal structure). For the reliable determination and assessment of behaviour and effects of nanomaterials as well as for the determination of the exposure of humans and environment a comprehensive physical-chemical characterization of nanomaterials is essential. This is an important prerequisite to identify them as nanomaterials and to interpret and compare test results and - in future – to forecast interaction and effects of nanomaterials. In 2006, the OECD launched a sponsorship program for the testing of nanomaterials in which 11 nanomaterials were thoroughly investigated using a variety of methods. The aim of the project was, among other things, to find out where problems occur and where there are gaps in the measurement and test procedures and where are changes required. An important outcome of the sponsorship program was the finding that the OECD Test Guidelines should in several cases be extended to the specific needs in testing of nanomaterials. The existing standardized test methods of the OECD for physical-chemical characterization have not been developed for nanomaterials in particular. A high demand for an extension of the test guidelines was identified. Germany complied with the OECD's request in 2017 and has agreed to extend the “Test Guideline on Particle Size Distribution / Fiber Length and Diameter Distributions Test Guideline” for Manufactured Nanomaterials (MN). UBA commissioned BAM and BAuA with the preparation of the Test Guideline. The aim of the project is the development of a harmonized test protocol for a valid and reproducible determination of particle size and size distribution which is one of the most relevant physical-chemical properties for MNs. Different measuring methods provide different results for the size distribution of the particles. This is caused by the different measuring principles of the methods. Each method measures a specific parameter that ultimately determines particle size. First, the measured quantity differs for each method (Scattered light intensity, 2D image / projection, electric mobility, etc.). Second, the calculated diameters of the MN may differ (Feret Diameter, Area Projection, Mobility Diameter, Aerodynamic Diameter, Hydrodynamic Diameter). Third, a measuring method provides a size distribution which is measured either mass-based, surface-based or number-based. A conversion between the results requires additional parameters and thus possibly increases the measurement error. In addition to the technical differences, the individual parameters are strongly influenced by the structure and material of the nanoparticles. For example, a surface functionalization can lead to very different results in the size distribution. The suitability of measurement methods differs with the material of the MN. As a result, two very different results can be measured for the particle size distribution using two different methods, which are nevertheless both correct. Several large projects in recent years therefore concluded that nanomaterials should be characterized by at least two complementary method. Imaging techniques are regarded as one of these methods for the characterization, the complementary methods are supposed to be statistical methods. The different results for the size distribution of nanomaterials become problematic for the registration of new MN. A comparable and reproducible size distribution is a prerequisite for a standardized registration. In the future, the particle size distribution in the EU will also decide on the classification of a substance as a nanomaterial or as a non-nanomaterial. Especially in borderline cases, a standardized and comparable measurement methodology is therefore essential. T2 - BAM-PTB Workshop 2018 CY - Berlin, Germany DA - 14.05.2018 KW - Nano KW - OECD KW - Test Guideline KW - Size distribution KW - Particle PY - 2018 AN - OPUS4-45106 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kämpf, K. A1 - Bachmann, V. A1 - Bresch, Harald A1 - Schmidt, Alexandra A1 - Kuhlbusch, T. A1 - Schwirn, K. A1 - Völker, D. T1 - OECD Test Guideline on particle size and particle size distribution of manufactured nanomaterials: simultaneous measurement of length and diameter of fibers N2 - The new OECD test guideline will address the following four main steps in the determination of the length and width distributions of fibers: sample preparation, image acquisition, data evaluation and uncertainty analysis. As the sample preparation has to be optimized for each material, general quality criteria will be given in the protocol. For full visibility of a fiber the appropriate resolution has to be chosen. In the data evaluation the length and diameter of each fiber will be determined concurrently to allow for application of different regulatory definitions. The quality of the results critically depends on the sample preparation as well as the data evaluation. In this step the classification rules have to be formulated and followed accurately in order to optimize reproducibility of the method. The SOP will be validated in an international round robin test, which is planned for 2018/2019. T2 - BAM-PTB-Workshop 2018 CY - Berlin, Germany DA - 14.05.2018 KW - Nano KW - Fibre KW - Fiber KW - OECD KW - Size distribution PY - 2018 AN - OPUS4-45107 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schwirn, K. A1 - Völker, D. A1 - Ahtiainen, J. A1 - Schmidt, Alexandra A1 - Bresch, Harald A1 - Kämpf, K. A1 - Bachmann, V. A1 - Kuhlbusch, T. T1 - OECD Test Guidelines development for chemicals safety assessment of nanomaterials N2 - The OECD test guidelines (TGs) for testing chemicals have been widely used for regulatory purposes all over the world since the establishment of the Mutual Acceptance of Data (MAD) principle in 1984. This MAD principle ensures that, if a chemical is tested under the Good Laboratory Practice (GLP) conditions accordingly to an OECD TG, the data should be accepted in all OECD countries. The TGs have been developed, harmonized, internationally validated (round robin tests) and adopted by OECD countries to be used for the physical-chemical characterisation, fate estimation, and hazard identification for risk assessment of various chemicals. In addition to the TGs, OECD Guidance Documents (GDs) usually provide guidance on how to use TGs and how to interpret the results. These GDs do not have to be fully experimentally validated, and hence they are not under MAD, but they are based on relevant published scientific research. But are the existing TGs and the related GDs applicable and adequate for the regulatory testing of nanomaterials? In general, it is accepted that most of the "endpoints" or more precisely measurement variables are applicable also for nanomaterials. However, for some endpoints new or amended TGs are needed. In addition, several GDs are needed to give more precise advice on the test performance in order to gain regulatory relevant data on nanomaterials. The poster will present the status quo on recent TGs and GDs development for nanomaterials at OECD level with relevance for physical-chemical characterisation. Emphasis will be given to the proposed OECD TG on particle size and size distribution for manufactured nanomaterials. The development of such a TG is of special importance as particle size and size distribution is considered as major information for nanomaterial identification and characterization. A reliable and reproducible characterisation of particle size and size distribution is also needed for chemicals risk assessment of nanomaterials, for instance to interpret and compare test results and - in future – to forecast interaction and effects of nanomaterials. The presented poster will illustrate the way from the idea for a new TG and new GD to an accepted OECD TG/GD. T2 - BAM-PTB Workshop 2018 CY - Berlin, Germany DA - 14.05.2018 KW - Nano KW - OECD KW - Nanomaterials KW - Test Guideline KW - Prüfrichtlinie PY - 2018 AN - OPUS4-45108 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Adam, Christian A1 - Krüger, Oliver ED - IWA Publishing, T1 - Wastewater as a resource: From rare earth metals to phosphorus N2 - Wastewater contains a diverse array of organic and inorganic compounds and its complex composition strongly depends on the location and the connected dischargers. However, municipal wastewater as a carrier of feces and urine generally contains considerable amounts of the main nutrients nitrogen and phosphorus. The latter is in the focus of the discussions about the recovery potential of wastewater due to the relatively high mass flows of phosphorus in wastewater and the finite nature and decreasing quality of phosphate rock reserves. But due to the presence of the whole periodic table of elements, wastewater might contain further valuable components of interest for recovery including those defined as critical raw materials by the European Commission. Phosphorus and most of the other critical raw materials are fixed in the sewage sludge and after incineration in the sewage sludge ash (SSA). This is accompanied by high concentration factors from wastewater via sludge to ash. However, the mass fractions of the majority of elements in sewage sludge are comparable to those of the earth crust, indicating no relative enrichment. Nevertheless, enrichment factors of 100 or higher are given for phosphorus, copper, zinc, cadmium, silver, tin, lead and the platinum group elements indicating an anthropogenic input. An economic value of sewage sludge was estimated to $460,-/t calculated on the basis of the respective market prices for high purity elements – a theoretical value. A German survey of sewage sludge ashes showed that the mass fractions and the mass flows of most of the elements present in SSA are probably too low for an economic recovery. In most cases the mass flows are rather small compared to the imports and the chemical forms are not suitable for recovery. An exception is phosphorus that is present in high mass fractions up to 13% and that bears a high substitution potential. If the application of P-recovery technologies lead to a further concentration of valuable elements e.g. as by-products in side streams of the process, it would probably make also the recovery of other elements of economic interest. KW - Wastewater KW - Sewage sludge PY - 2018 SN - 978-1-78040-835-4 SN - 978-1-78040-836-1 SN - 978-1-78040-954-2 DO - https://doi.org/10.2166/9781780408361 SP - 241 EP - 252 PB - IWA Publishing CY - London AN - OPUS4-45679 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koerdt, Andrea A1 - An, Biwen Annie A1 - Özcan Sandikcioglu, Özlem A1 - Widdel, F. T1 - Microbial corrosion of iron coupled to methanogenesis by strains from different environments N2 - Microbially influenced corrosion (MIC) of iron is usually attributed to sulfate-reducing microorganisms (SRM) which act upon the metal by the reactiveness of hydrogen sulfide, and by withdrawal of the available electrons (Fe → Fe²⁺ + 2e⁻ ; E° = 0.47 V) in electrical contact through surface attachment. Also methanogenic archaea are supposed to cause MIC. Because they do not produce hydrogen sulfide, withdrawal of electrons may be their main corrosive mechanism; however, mechanistic details and kinetics of the overall process are poorly understood. Precipitation of siderite (4Fe + 5HCO₃⁻ + 5H⁺ → 4FeCO₃ + CH₄ + 3H₂O) can lead to an insulating layer on the metal surface and lower the corrosion rate. Still, the extent of FeCO₃ precipitation may be significantly influenced by environmental conditions such as pH and advective processes. To investigate the corrosive potential of methanogens, we studied strains isolated from marine sediments (Methanococcus maripaludis 14266, 2067, Methanobacterium-affiliated strain IM1), crude oil tanks (Methanococcus maripaludis Mic1c10, KA1) and the oral cavity (Methanobrevibacter oralis) in a closed (batch) culture, and in a sand-packed flow-through cell with pH control and simulation of a fluctuating environment. Results indicate that the rates of iron corrosion due to coupled methanogenesis (up to 0.3 mm/yr) are comparable to that caused by SRM. Surface analyses of the metal showed severe pitting. Such knowledge and deeper understanding also from an electrokinetic point of view may not only provide further models in microbial electrophysiology, but also contribute to mitigation strategies in MIC. T2 - ISME 17th International Symposium on Microbial Ecology CY - Leipzig, Germany DA - 12.08.2018 KW - Corrosion KW - Methanogens KW - Biofilm KW - Flow-System KW - Iron PY - 2018 AN - OPUS4-45735 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Auersch, Lutz T1 - Simple and fast prediction of train-induced track forces, ground and building vibrations N2 - A simple and fast prediction scheme is presented for train induced ground and building vibrations. Simple models such as (1-dimensional) transfer matrices are used for the vehicle-track-soil interaction and for the building-soil interaction. The wave propagation through layered soils is approximated by a frequency-dependent homogeneous half-space. The prediction is divided into the parts “emission” (excitation by railway traffic), “transmission” (wave propagation through the soil) and “immission” (transfer into a building). The link between the modules is made by the excitation force between emission and transmission, and by the free-field vibration between transmission and immission. All formula for the simple vehicle-track, soil and building models are given in this article. The behaviour of the models is demonstrated by typical examples, that is the mitigation of train vibrations by elastic track elements, the low- and high-frequency cut-offs characteristic for layered soils, and the interacting soil, wall and floor resonances of multi-storey buildings. It is shown that the results of the simple prediction models can well represent the behaviour of the more time-consuming detailed models, the finite-element boundary-element models of the track, the wavenumber integrals for the soil, and the three-dimensional finite-element models of the building. In addition, measurement examples are given for each part of the prediction confirming that the methods provide reasonable results. As the prediction models are fast in calculation, many predictions can be done, for example to assess the environmental effect along a new railway line. The simple models have the additional advantage that the user needs to know only a minimum of parameters. So, the prediction is fast and user-friendly, but also theoretically and experimentally well-founded. KW - Railway induced vibration KW - Ground vibration KW - Layered soil KW - Building response KW - Excitation forces KW - Track and vehicle irregularities PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-513340 DO - https://doi.org/10.1007/s40534-020-00218-7 SN - 2662-4745 VL - 28 IS - 3 SP - 232 EP - 250 PB - Springer AN - OPUS4-51334 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Winkler, Nicolas P. A1 - Neumann, Patrick P. A1 - Hüllmann, Dino A1 - Kohlhoff, Harald A1 - Bartholmai, Matthias A1 - Bennetts, V. H. A1 - Lilienthal, A. J. T1 - Remote gas sensing with multicopter platforms N2 - This presentation gives an introduction to the gas-sensitive aerial robots developed at BAM, including various application examples in the field of mobile robot olfaction: gas source localization and gas distribution mapping. T2 - Zweites Innovationsforum "Autonome, mobile Dienste; Services für Mobilität" CY - Berlin, Germany DA - 04.06.2019 KW - Localization of gas sources KW - Mobile Robot Olfaction KW - Nano UAV Swarm KW - Tomographic reconstruction of gas plumes KW - Tunable Diode Laser Absorption Spectroscopy (TDLAS) KW - UAV-REGAS PY - 2019 AN - OPUS4-48167 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schumacher, Julia T1 - Photoperception in black fungi that share niches with green organisms N2 - Sunlight, the major source of energy, drives life but in excess it provokes UV-induced DNA damage, accumulation of reactive oxygen species, desiccation, osmotic stresses and so on. Biological strategies to survive excess light include protection, avoidance and active utilisation. Phototrophic organisms including cyanobacteria, green algae and plants use all three strategies. Fungi occupying phototrophic niches may profit from the surplus photosynthetic products which also obliges them to cope with the same light-induced stresses. One way of handling these stresses would be to use similar signalling pathways to sense the presence and to interact with their phototrophic partners. Obviously, the levels of adaptation and responses to light will depend on the environment as well as fungal genotype and phylogenetic position. Black fungi – a polyphyletic group – accumulate the dark pigment DHN melanin in their cell walls and often occupy light-flooded habitats from phyllosphere to rock surfaces. Here we compare two sequenced melanised fungi of different lifestyles in their response to light. The Leotiomycete Botrytis cinerea is an aggressive pathogen that primarily infects the above-ground parts of plants. It possesses large numbers of photoreceptors that respond to a broad spectrum of light. As a consequence, light controls morphogenesis in which it induces conidiation for disease spreading and represses sclerotial development for survival and/or sexual recombination. Cellular components involved in photo-perception and regulation of morphogenesis, stress responses and virulence have been identified and appear to regulate propagation, survival and infection. These include phytochromes, a group of photoreceptors which are particularly enriched in the Leotiomycetes and that mediate coordinated responses to light and elevated temperatures. Assuming that photo-regulation may be equally important for fungi that live in mutualistic relationships with phototrophs either by forming composite organisms or biofilms, we investigate the role of light in the rock-inhabiting Eurotiomycete Knufia petricola. Like other black yeasts, K. petricola grows slowly, does not form specialised reproductive structures and constitutively produces DHN melanin as well as carotenoids. Combining K. petricola with the cyanobacterium Nostoc punctiforme, we developed a model system for studying biofilm formation and bio-weathering. A genetic toolbox to manipulate this model system is being developed. K. petricola strain A95 possesses ten putative photoreceptors, more than found in filamentous Eurotiomycetes suggesting that light plays an important role for abiotic and biotic interactions in extremo-tolerant and symbiosis-capable fungi. T2 - International Symposium on Fungal Stress (ISFUS) CY - Sao Jose dos Campos, Brazil DA - 19.05.2019 KW - Botrytis KW - carotenoids KW - Knufia KW - melanin KW - Nostoc PY - 2019 AN - OPUS4-48130 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Weimann, Karin A1 - Adam, Christian T1 - Advantages of recycling gypsum plasterboards N2 - During the last decades the material composition of buildings has become increasingly diverse. However, largely sorted material flows are needed for generating high quality secondary building materials. The use of secondary building materials can meet the requirements of sustainability in several ways: the extended time availability of primary raw materials and, thereby, the preservation of natural resources as well as the conservation of landfill sites. Recycling of gypsum (calcium sulfate) can be a good example for the environmental benefits of closed-loop recycling. The content of sulfates in other secondary building materials, in particular in recycled concrete aggregates, should be minimized for quality reasons. In contrast, separated gypsum can also be used in gypsum production if the high quality requirements for the recycled gypsum are met. Since almost all processing steps in the recycling process are associated with environmental impacts, an environmental evaluation of the use of recycled gypsum as a substitute in gypsum production has to be carefully conducted. This paper focusses on the techniques for generating recycled gypsum from gypsum plasterboards, the related quality requirements and a comprehensive environmental evaluation of the complete process. T2 - IV International Conference Progress of Recycling in the Built Environment CY - Lisbon, Portugal DA - 11.10.2018 KW - Environmental evaluation KW - CDW processing KW - Gypsum PY - 2018 AN - OPUS4-48079 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Neumann, Patrick P. A1 - Hirschberger, Paul A1 - Baurzhan, Zhandos A1 - Tiebe, Carlo A1 - Hofmann, Michael A1 - Hüllmann, Dino A1 - Bartholmai, Matthias T1 - Indoor air quality monitoring using flying nanobots: Design and experimental study N2 - In this paper, we introduce a nano aerial robot swarm for Indoor Air Quality (IAQ) monitoring applications such as occupational health and safety of (industrial) workplaces. The robotic swarm is composed of nano Unmanned Aerial Vehicles (UAVs), based on the Crazyflie 2.0 quadrocopter, and small lightweight Metal Oxide (MOX) gas sensors for measuring the Total Volatile Organic Compound (TVOC), which is a measure for IAQ. An indoor localization and positioning system is used to estimate the absolute 3D position of the swarm similar to GPS. A test scenario was built up to validate and optimize the swarm for the intended applications. Besides calibration of the IAQ sensors, we performed experiments to investigate the influence of the rotor downwash on the gas measurements at different altitudes and compared them with stationary measurements. Moreover, we did a first evaluation of the gas distribution mapping performance. Based on this novel IAQ monitoring concept, new algorithms in the field of Mobile Robot Olfaction (MRO) are planned to be developed exploiting the abilities of an aerial robotic swarm. T2 - 18th International Symposium on Olfaction and Electronic Nose CY - Fukuoka, Japan DA - 26.05.2019 KW - Nano aerial robot KW - Swarm KW - Indoor air quality KW - Monitoring PY - 2019 SN - 978-1-5386-3641-1 SP - 1 EP - 3 PB - IEEE CY - USA AN - OPUS4-48148 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Neumann, Patrick P. A1 - Hirschberger, Paul A1 - Baurzhan, Zhandos A1 - Tiebe, Carlo A1 - Hofmann, Michael A1 - Hüllmann, Dino A1 - Bartholmai, Matthias T1 - Indoor air quality monitoring using flying nanobots: Design and experimental study N2 - In this paper, we introduce a nano aerial robot swarm for Indoor Air Quality (IAQ) monitoring applications such as occupational health and safety of (industrial) workplaces. The robotic swarm is composed of nano Unmanned Aerial Vehicles (UAVs), based on the Crazyflie 2.0 quadrocopter, and small lightweight Metal Oxide (MOX) gas sensors for measuring the Total Volatile Organic Compound (TVOC), which is a measure for IAQ. An indoor localization and positioning system is used to estimate the absolute 3D position of the swarm similar to GPS. A test scenario was built up to validate and optimize the swarm for the intended applications. Besides calibration of the IAQ sensors, we performed experiments to investigate the influence of the rotor downwash on the gas measurements at different altitudes and compared them with stationary measurements. Moreover, we did a first evaluation of the gas distribution mapping performance. Based on this novel IAQ monitoring concept, new algorithms in the field of Mobile Robot Olfaction (MRO) are planned to be developed exploiting the abilities of an aerial robotic swarm. T2 - 18th International Symposium on Olfaction and Electronic Nose CY - Fukuoka, Japan DA - 26.05.2019 KW - Nano aerial robot KW - Swarm KW - Indoor air quality KW - Monitoring PY - 2019 AN - OPUS4-48149 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Neumann, Patrick P. A1 - Hüllmann, Dino A1 - Kohlhoff, Harald T1 - Aerial- and ground-based gas tomography for increasing environmental safety in chemical industry (AGATO) N2 - In AGATO, we combine ground and aerial robots into a heterogeneous robot swarm, equip robots with gas sensors (in-situ/open-path) and/or reflectors as payload, reconstruct potential gas plumes/clouds in 3D, and identify potential gas leaks based on reconstructed tomographic images. T2 - 18th International Symposium on Olfaction and Electronic Nose CY - Fukuoka, Japan DA - 26.05.2019 KW - Mobile Robot Olfaction KW - Tomographic reconstruction of gas plumes KW - Tunable Diode Laser Absorption Spectroscopy (TDLAS) KW - UAV-REGAS PY - 2019 AN - OPUS4-48150 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koerdt, Andrea A1 - An, Biwen Annie A1 - Kleinbub, Sherin T1 - Investigation of methanogen-induced microbiologically influenced corrosion (Mi-MIC) using simulated marine environments under flowing conditions N2 - Microbially influenced corrosion (MIC) of iron is usually attributed to sulfate-reducing microorganisms (SRM) which act upon the metal by the reactiveness of hydrogen sulfide, and by withdrawal of the available electrons in electrical contact with the metal. Methanogenic archaea can also cause MIC by directly withdrawing electrons from the iron surface for methanogenesis. However, the mechanistic details and kinetics of the overall process are poorly understood. Precipitation of siderite, a by-product of methanogenesis, (4Fe + 5HCO3 + 5H+  4FeCO3 + CH4 + 3H2O) can lead to an insulating layer on the metal surface and lower the corrosion rate. Still, the extent of FeCO3 precipitation may be significantly influenced by environmental conditions such as pH and advective processes. T2 - Dechema CY - Frankfurt a. M., Germany DA - 13.05.2019 KW - MIC KW - Corrosion KW - Methanogens KW - Corrosion product PY - 2019 AN - OPUS4-47982 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Büchele, Dominique A1 - Chao, Madlen A1 - Ostermann, Markus T1 - Development of a robust calibration model for determination of nutrients in soils using EDXRF N2 - As part of the BonaRes research initiative, funded by the BMBF, strategies are being developed to use soil as a sustainable resource in the bio economy. The interdisciplinary subproject I4S - “Intelligence for soil” - is responsible for the development of an integrated system for site-specific management of soil fertility. For this purpose, a platform is constructed and various sensors are installed. Real-time data will be summarised in models and decision-making algorithms will be used to control fertilisation and accordingly improve soil functions. Aim of the BAM in the frame of I4S is the characterisation of an energy-dispersive X-ray fluorescence (EDXRF) based sensor for robust determination of plant essential nutrients in soil. First a principal component analysis (PCA) was used to identify outliers and to observe the largest variance within the German soil samples. It could be monitored that splitting of the samples was due to their iron content. Given that clay samples contain high amounts of iron and sandy samples low amounts, a classification of the samples by their soil texture according to VD LUFA was possible. Considering the complex composition of soil, a matrix-specific calibration was carried out by univariate and multivariate data analysis. The figures of merit demonstrated that a more robust calibration model with negligible matrix effects can be obtained by a multivariate approach using partial least squares regression (PLSR). A better correlation between predicted values compared to reference values for German soil samples was observed for the chemometric calibration model than for the univariate one. Different factors can affect the received calibration models such as moisture and particle size distribution which is especially important due to later online Analysis. In first studies the influence of moisture on the detection of plant essential nutrients was investigated. With increasing water content, the characteristic fluorescence peaks decrease and start to increase again at a water content of 15 %. With lower moisture content the soil agglomerates which leads to lower packing of the sample, resulting in a rougher surface which negatively influence the signals. Whereas, agglomerates are not formed at higher water content. This allows the sample to be packed more tightly thus a smoother surface and a better homogeneity is obtained. Furthermore, particle size distribution leads to significantly higher uncertainties and lower signals when comparing grounded (< 500 μm) and not grounded (< 2 mm) samples. This can be explained by amplifying of the already known inhomogeneity of soils. Both factors must be included in the chemometric PLSR to obtain robust calibration models for each macro and micro nutrient. T2 - AK Prozessanalytik CY - Hannover, Germany DA - 03.12.2018 KW - EDXRF KW - Soil KW - Chemometrics PY - 2018 AN - OPUS4-48280 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Büchele, Dominique A1 - Chao, Madlen A1 - Ostermann, Markus T1 - Influence of moisture and grain sizes on the determination of nutriens in german agricultural soils using EDXRF N2 - As part of the BonaRes research initiative, funded by the BMBF, strategies are being developed to use soil as a sustainable resource in the bio economy. The interdisciplinary subproject I4S - “Intelligence for soil” - is responsible for the development of an integrated system for site-specific management of soil fertility. For this purpose, a platform is constructed and various sensors are installed. Real-time data will be summarised in models and decision-making algorithms will be used to control fertilisation and accordingly improve soil functions. Aim of the BAM in the frame of I4S is the characterisation of an energy-dispersive X-ray fluorescence (EDXRF) based sensor for robust determination of plant essential nutrients in soil. First a principal component analysis (PCA) was used to identify outliers and to observe the largest variance within the German soil samples. It could be monitored that splitting of the samples was due to their iron content. Given that clay samples contain high amounts of iron and sandy samples low amounts, a classification of the samples by their soil texture according to VD LUFA was possible. Considering the complex composition of soil, a matrix-specific calibration was carried out by univariate and multivariate data analysis. The figures of merit demonstrated that a more robust calibration model with negligible matrix effects can be obtained by a multivariate approach using partial least squares regression (PLSR). A better correlation between predicted values compared to reference values for German soil samples was observed for the chemometric calibration model than for the univariate one. Different factors can affect the received calibration models such as moisture and particle size distribution which is especially important due to later online analysis. In first studies the influence of moisture on the detection of plant essential nutrients was investigated. With increasing water content, the characteristic fluorescence peaks decrease and start to increase again at a water content of 15 %. With lower moisture content the soil agglomerates which leads to lower packing of the sample, resulting in a rougher surface which negatively influence the signals. Whereas, agglomerates are not formed at higher water content. This allows the sample to be packed more tightly thus a smoother surface and a better homogeneity is obtained. Furthermore, particle size distribution leads to significantly higher uncertainties and lower signals when comparing grounded (< 500 μm) and not grounded (< 2 mm) samples. This can be explained by amplifying of the already known inhomogeneity of soils. Both factors must be included in the chemometric PLSR to obtain robust calibration models for each macro and micro nutrient. T2 - BonaRes Statusseminar CY - Leipzig, Germany DA - 19.02.2019 KW - XRF KW - Mmoisture KW - Chemometrics PY - 2019 AN - OPUS4-48281 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Weimann, Karin T1 - Gypsum plasterboard recycling N2 - The use of secondary building materials can meet the requirements of sustainability in several ways: the extended time availability of primary raw materials and, thereby, the protection of natural ressources as well as the conservation of landfill sites. Regarding the predicted decrease of gypsum supply in Germany, particularly the recycling of gypsum (calcium sulfate) is of growing importance. Currently, the gypsum demand is fulfilled (at least 60%) by gypsum as side product from coal-fired power plants (FGD Gypsum). Germany’s natural gypsum deposits fulfil the remaining gypsum demand. Due to national climate protection goals the gypsum supply from coal power plants will decrease significantly in the future. In addition, the content of sulfates in other secondary building materials, in particular in recycled concrete aggregates, should be minimized for quality reasons. Separated gypsum can be used in gypsum production if the high quality requirements for recycled gypsum are met. Accordingly, there have been significant advancements in the processing of gypsum residues in the last years. Since almost all processing steps in the recycling process are associated with environmental impacts, an evironmental evaluation of the use of recycled gypsum as a substitute in gypsum production has to be carefully conducted. The presentation focusses on the techniques for generating recycled gypsum from gypsum plaster boards, the related quality requirements and a comprehensive environmental evaluation of the complete process. T2 - MINEA Workshop: Recovery Potential of Construction and Demolition Waste CY - Brussels, Belgium DA - 21.03.2019 KW - Gypsum KW - CDW processing KW - Environmental evaluation PY - 2019 AN - OPUS4-48220 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Torge, Manfred A1 - Seneschal-Merz, Karine A1 - Günther, Tobias A1 - Wilsch, Gerd T1 - Determination of boron in 19th and 20th century paint layers using LIBS N2 - 19th and 20th centuries glass paint layers consist of a colour body and a colourless lead silicate flux, in which borax or boric acid was added as further component to improve the paint ability and to reduce the firing temperature for multiple layers of paint. Model glasses were used in laboratory tests to investigate the stability of glass paints with additions of boron oxide. To determine boron in paint layers, a LIBS-system with pulsed NdYAG-laser was used. T2 - Technart 2019 CY - Brugge, Belgien DA - 07.05.2019 KW - Stained glass windows KW - Glass paints KW - LIBS PY - 2019 AN - OPUS4-48229 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Geburtig, Anja T1 - Possibilities on prediction of plastic’s fragmentation time - Digital twins for decomposing-time predictions N2 - Depending on the specific plastic’s ageing sensitivities, the durations which plastic components remain in the environment can be very long. As, in the past, the advantages in ageing resistance and durability were highly evaluated, we now face the problem of quite stable plastic waste within the environment. However, there is only little knowledge on the real timescales until macroscopic fragmentation for the different kinds of plastic under various environmental conditions. Here, weathering methods are presented, which have been used for the failure prediction in specific outdoor conditions. Issues of uncertainty, reproducibility, or validation are discussed. For the prediction of the plastic’s fragmentation, much longer time scales have to be considered. To do this within a limited project life span, various processes as well as their acceleration potential have to be evaluated separately, such as temperature increase or spectral shift. The possibilities and limitations of such controlled acceleration will be discussed. T2 - 29th IAPRI Symposium on Packaging CY - Twente, Enschede, The Netherland DA - 11.06.2019 KW - Digital twin KW - Plastic KW - Ageing KW - Environment KW - Fragmentation PY - 2019 SN - 978-90365-4731-4 SP - 630 EP - 631 PB - University of Twente CY - Twente, Enschede, The Netherlands AN - OPUS4-48238 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Eisentraut, Paul A1 - Hassanein, Yosri A1 - Elert, Anna Maria A1 - Braun, Ulrike T1 - Understanding degradation mechanisms of microplastics in environmental samples N2 - Im Vortrag wird die Problematik Mikroplastik eingeführt und ein Probenset aus dem Mittelmeer mit ersten Ergebnissen besprochen. Die Alterung von Polymeren im Umweltkontext sowie Möglichkeiten der Analyse von Polymeralterungsfortschritten werden diskutiert. Die thermoanalytische Methode mit Zersetzungsgasanalytik (TED-GC-MS) wird eingeführt und deren Einsatzmöglichkeiten in der Thematik umrissen. T2 - EuroMech Colloquium 607 Marine Aging of Polymers CY - Brest, France DA - 28.08.2019 KW - Microplastics KW - Tara Mediterranee KW - Polymer aging KW - TED-GC-MS PY - 2019 AN - OPUS4-49110 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schraut, Katharina A1 - Adamczyk, Burkart A1 - Simon, Sebastian A1 - von Werder, Julia A1 - Meng, Birgit A1 - Stephan, D. A1 - Kargl, F. A1 - Adam, Christian T1 - Formation and chemical stabilisation of tricalcium-silicate during solidification from the melt of post-treated metallurgical slags N2 - Tricalcium-silicate (C3S) or Alite is the most important mineral in Portland cement. Since pure tricalcium-silicate is only stable above temperatures of 1250 °C, its decomposition has to be prevented technically by fast cooling after the sintering process. At room temperature, the decomposition velocity is very slow so that metastable tricalcium-silicate is obtained. Although the mechanisms of clinker phase formation during burning process of Portland cement in a rotary kiln were solved and improved over the years, in view of possible economic and ecological benefits current projects aim to produce clinker phases from metallurgical slags. Recent studies discovered that the mineral phase which remained after a reducing treatment and separation of formed metallic iron from molten Linz-Donawitz (LD-) slags contained about 60 wt.% Alite despite it was cooled slowly. Because the results could be verified using slags from different origins and varying cooling velocities a chemical stabilisation of the Alite can be assumed. First tests in mortars indicate that workability, hardening and solid state properties are comparable with an ordinary Portland cement. An application of the observed phenomenon in cement production requires enhanced knowledge about formation and stabilisation conditions of Alite during crystallisation from melts in contrast to the sintering reactions in conventional Portland cement production. Therefore, this study focuses on the stabilisation mechanisms of Alite in consolidating melts. Samples from different melting experiments are analysed to determine stabilising factors. T2 - 15th International Congress on the Chemistry of Cement CY - Prag, Czech Republic DA - 16.09.2019 KW - Tricalcium-silicate KW - Portland Cement KW - Alite KW - Steelmaking slag PY - 2019 SP - Paper 492, 1 EP - 10 AN - OPUS4-49050 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Müller, Axel A1 - Vogler, M. A1 - Grathwohl, P. A1 - Kocher, B. A1 - Braun, Ulrike T1 - Analysis of tire wear particles in soil samples from roadside using TED-GC/MS N2 - Tire wear particles (TW) are generated by the abrasions of tires on the road surface through traffic. These particles can be transported by air and surface runoff and might also infiltrate the soil and consequently affect terrestrial ecosystems. The estimated tire wear (TW) emissions are immense, with 1.33 106 t a-1 in Europe. Despite this, only little is known about the environmental contents or the fate of TW. One reason for this knowledge gap is the challenging analysis of TW in environmental samples. Detection of TW with spectroscopic methods is problematic due to high fluorescence interferences caused by contained black carbon. One analytical approach is to use zinc (Zn), a typical additive in tires, as a specific marker for the quantification of tire wear. However, any Zn originating from the sample matrix must be separated beforehand and requires elaborate sample preparation. Car tires consist partly of synthetic rubbers, such as styrene-butadiene-rubber (SBR). This SBR could be identified and quantified via Thermal-Extraction-Desorption-Gas Chromatography-Mass Spectrometry (TED-GC-MS). This newly developed and fast screening method allows the simultaneous detection of microplastics and TW mass contents and requires minimal to no sample preparation. Firstly the sample is thermally extracted in a thermobalance under a nitrogen atmosphere. The resulting specific decomposition products are sorbed on a solid phase adsorber, which is then transferred to a GC-MS via an autosampler, where the products are desorbed, separated and identified. Cyclohexenylbenzene is used as a specific marker for SBR. Here we investigated top layer soil samples, collected at the roadside of highly frequented German highways. Samples were analyzed without sample preparation, and SBR was detected in all investigated samples in mass contents ranging from 67.2 to 2230 mg kg-1. A correlation between SBR and Zn content in the soil was confirmed, while the correlation between SBR and Corg was hardly pronounced. We successfully demonstrated the application of TED-GC-MS as a screening method for tire wear in soil samples. The present study will discuss these analytical results in detail as well as sampling parameters like sampling depth and distance to the roadside, and the effect of the particle size on the particle transport by water runoff and air. T2 - SETAC 2020 SciCon CY - Online meeting DA - 03.05.2020 KW - Microplastic KW - Analysis KW - Tire wear KW - Soil PY - 2020 AN - OPUS4-51086 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - THES A1 - Vandrich, Jasmina T1 - Metabolic engineering in Halomonas elongata N2 - Halomonas elongata is a halophilic γ-proteobacterium that synthesizes and accumulates the compatible solute ectoine to cope with osmotic stress in saline environments. Ectoine possesses protecting properties and stabilizes proteins as well as whole cells against stresses like ionizing radiation and cytotoxins. These properties make ectoine a highly demanded ingredient in cosmetics and pharmaceuticals. To date H. elongata is the industrial Producer strain of ectoine, but several metabolic factors for optimum ectoine production remain to be explored. In this work, we used up to date Metabolic engineering approaches following the ‘Push, Pull, Block – strategy’ to examine targets that contribute to ectoine synthesis. Firstly, the basics of glucose catabolism were inspected to PUSH and enhance carbon flow towards ectoine synthesis. Secondly, lysine biosynthesis was targeted to BLOCK a pathway that is competing for precursors with ectoine synthesis. Thirdly, the mechanosensitive (MS) channels of H. elongata have been examined as possible excretion routes for ectoine. An overexpression of the ectoine excretion channels potentially could PULL out product at the end of ectoine synthesis and increase overall ectoine flux. For the interrogation of central metabolic pathways, we established the new molecular tool CRISPR-mediated interference (CRISPRi) for targeted modulation of gene expression. PUSH Glucose catabolism through the Entner-Doudoroff (ED) and Emden-Meyerhof-Parnas (EMP) pathway was targeted with CRISPRi and examined on gene expression level for ist response to changing salinity and different carbon sources. Changing salinity did not influence gene expression levels of glucose catabolism but the carbon source glucose triggered glycolysis through the (ED) pathway. When gene expression of the ED pathway was downregulated with CRISPRi, the growth rates remained constant. The observations indicate a metabolic overflow mechanism for glycolysis, in which fluxes are constantly high - even at lower salinity when no resources are demanded for ectoine synthesis. The further analysis of glucose to product conversion rates will advise optimum conditions for future industrial cultivation processes. BLOCK Lysine biosynthesis was downregulated with CRISPRi, which led to a significant increase in ectoine production. Hence, the blockage of lysine biosynthesis would be a valuable strategy for the optimization of the industrial producer strain in future studies. PULL MS channels and ectoine regulation are inevitably connected in osmoadaptation. Therefore, ectoine excretion, growth performance and gene expression levels of the MS channels were monitored in steady state conditions and in response to osmotic shock in the wildtype strain and in a MS channel deletion mutant. We observed that the MS channels were essential for the survival of osmotic shock but surprisingly their presence reduced cell growth under high salinity. The MS channels were only partially responsible for ectoine excretion. Thus, alternative ectoine excretion channels must exist and remain to be explored. KW - Halomonas elongata KW - Ectoine KW - CRISPR dCas9 PY - 2019 SP - 1 EP - 94 CY - Potsdam AN - OPUS4-51094 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Erning, Johann Wilhelm A1 - Bobzien, M. T1 - Corrosion behaviour of printed 1.4404 in drinking water systems N2 - The regulations for materials in contact with drinking water require tests to evaluate the hygienic properties of the materials. A printed 1.4404 was tested according to EN 16056 and the suitability for the use in drinking water was proven. T2 - Eurocorr 2020 CY - Online meeting DA - 07.09.2020 KW - Passivity KW - Drinking water KW - Stainless steel KW - Additive manufacturing PY - 2020 AN - OPUS4-51237 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Neumann, Patrick P. A1 - Hüllmann, Dino A1 - Winkler, Nicolas P. A1 - Kohlhoff, Harald A1 - Bartholmai, Matthias A1 - Hirschberger, Paul A1 - Bennetts, V.H. A1 - Lilienthal, A.J. T1 - Aerial Robot Olfaction - A Summary and Survey from BAM Perspective N2 - This presentation gives an introduction to the gas-sensitive aerial robots developed at BAM, including various application examples in the field of mobile robot olfaction: gas source localization and gas distribution mapping. T2 - Harvard University School of Engineering and Applied Sciences (SEAS)/Earth and Planetary Sciences (EPS) - Martin Group Seminar CY - Online meeting DA - 11.06.2020 KW - Mobile Robot Olfaction KW - Localization of gas sources KW - UAV KW - Tomographic reconstruction of gas plumes KW - Tunable Diode Laser Absorption Spectroscopy (TDLAS) PY - 2020 AN - OPUS4-50916 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Merryweather, S. A1 - Zweifel, D. A1 - Woydt, Mathias ED - Franek, F. ED - Bartz, Wilfried J. ED - Pauschitz, A. ED - Vizintin, J. ED - Ciulli, E. ED - Crockett, R. T1 - Fuel economy of engine oils based on polyalkylene glycols N2 - The driver for automotive OEMs to work on alternative engine oils is the need for fuel economy and no adverse effect of the engine oil on the durability of particulate filters and catalysts. The alternative engine oils should for mid-term also comply with a positive environmental Profile. T2 - 3rd European Conference on Tribology and 4th Vienna International Conference on Nano-Technology CY - Vienna, Austria DA - 07.06.2011 KW - Biomotox KW - Head capacity KW - Fuel economy KW - Drain KW - Polyglycol KW - Polypropylene glycol KW - Polyalkylene glycol KW - Polybutylene glycol PY - 2011 SN - 978-3-901657-38-2 SP - 25 EP - 34 PB - MDH Media GesmbH CY - Wien AN - OPUS4-35671 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Adam, Christian A1 - Herbeck, Maximilian T1 - Metal recovery by reductive melting of red mud N2 - Investigation about metal recovery from red mud by reductive smelting. T2 - Exchange of good practices on metal by-products recovery CY - Brussels, Belgium DA - 12.11.2015 KW - Red mud KW - Metal recovery KW - Reductive smelting PY - 2015 AN - OPUS4-35691 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Adam, Christian T1 - Phosphorus recovery from waste streams N2 - Presentation of all relevant recovery technologies from wastes T2 - Vorlesung im Rahmen der integrierten Veranstaltung "Advanced Recycling Technologies" der TU Berlin CY - Berlin, Germany DA - 19.01.2016 KW - Phosphorus recovery PY - 2016 AN - OPUS4-35694 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Hoffmann, Dirk A1 - Niesel, Konrad T1 - Effect of air pollutants on renderings and moisture-transport phenomena in masonry N2 - Determining a pore structure’s mechanical and chemical data on lime rendering exposed outdoors and including masonry in the laboratory, with regard to their behaviour on moisture transport and weathering was the objective. In search of clues for a dose-response relation - i.e. upon a connection between the emission rate of SO2 and NOx on one hand and the degree of damage to mainly plaster having a lime component on the other - it has been of interest to what extent their structural characteristics including pore space are modified and so consequently also physico-technical properties and chemical composition. For determining corresponding criteria, a widely diversified spectrum of test methods such as to obtain data concerning porosity and moisture transport, mechanical behaviour, and chemistry as well is available. A description of the manufacture of plate-shaped rendering specimens with lime putty, industrially hydrated lime and dolomitic hydrate (the last two also having a cement admixture) and also hydraulic lime as binding agents is included. First deposition velocity and absorption rate for SO2 were determined in laboratory tests on these materials in dry and moist states. After their exposure on outdoor racks at six locations in the Berlin region with differing SO2 atmospheric contents over at least 5 years, the samples of course show timedependent modifications of chemical and physical qualities which are especially reflected in the form of profiles of structure, strength and concentration, e.g. for sulphate. Local climatic differences, however, partly exert a greater influence on material than emissions. Similar investigations were performed on samples of comparable composition exposed 4 and 17 years. But the real surprise is furnished by the renderings’ behaviour depending upon orientation and upon height at a building. Since weathering behaviour and moisture transport (absorption, storage, release) are directly interdependent, further criteria are necessary for a critical assessment of a plasters’ pore structure. Starting from sands, plasters were characterized. The structural parameters were lastly influenced by the respective contents of sand, binder and mixing water. These results and those gained from bricks naturally find their expression in masonry sections manufactured there from, and above all in their capillary and evaporative behaviours, and also when applying different test liquids. Summary and cited literature is given at the end of this report. KW - Building Material KW - Mercury Intrusion Porosimetry KW - Gas Adsorption PY - 2008 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-356780 SP - 1 EP - 126 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-35678 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Vogler, Nico A1 - Barthel, Maria A1 - Kühne, Hans-Carsten T1 - Self-cooling concrete pavers – performance tests by long-term studies under various climatic conditions N2 - In the last several decades, the rural exodus has led to an increasing number of inhabitants in the urban areas. The increased building and infrastructural construction caused the transformation of the landscape and to an increased land sealing in these areas. Consequently, the increased land sealing influences the air temperatures in the cities, since houses, streets and squares heat up continuously. As a result, the city and metropolitan areas became significantly warmer than their surroundings - the effect is known as Urban Heat Island (UHI). These changes have a negative impact for the quality of life. To deal with the negative effects of UHI, a high level of technical and financial effort is necessary. The costs caused by the UHI effects are in the range of several billion US dollars worldwide per year. The recent studies on UHI showed that the established methods like parks and green spaces or bright coatings for roof areas will soon not be able to effectively cope with the UHI effect in urban areas. Therefore there is a need for additional methods to mitigate UHI effect in the cities. The streets, sidewalks and squares represent approximately 30% to 40% of the inner cities areas. If these areas are designed functionally, they can have a significant impact on the UHI. This contribution focuses on development of a concrete paving stone with self-cooling properties. For the cooling effect the evaporative cooling is used. The paving stone is able to store large quantities of water and deliver during appropriate environmental conditions. This paper deals with the results of the long-term experiments on the test-fields under different climatic conditions. To interpret the test-field-results, laboratory tests were carried out as well. The paper presents and discuss the obtained results, and points out the difficulties occurred. T2 - 2nd International conference on advances in cement and concrete technology in Africa CY - Dar es Salaam, Tanzania DA - 27.01.2016 KW - Urban heat island KW - Concrete paving stone KW - Self-cooling KW - Performance tests KW - Long-term studies KW - Hitzeinsel KW - Betonpflasterstein KW - Selbstkühlend KW - Nachweis der Wirksamkeit KW - Langzeitversuch PY - 2016 SP - 595 EP - 602 AN - OPUS4-35679 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dengel, Jörg A1 - Lohrer, Christian A1 - Stein, F. A1 - Perriam, J. T1 - Investigations on aerosol emissions of pyrotechnic smoke generators N2 - Pyrotechnic smoke generators fall under the Directive 2013/29/EU in Europe and could potentially belong to all categories except consumer fireworks. These types are especially present in the field of “Other Pyrotechnic Articles” of the categories P1 and P2, e.g. as simulation devices for paintball or airsoft gaming. The specific users of these products are aware of the corresponding smoke liberation during use and usually wear protective equipment to minimize exposure to potentially harmful aerosols. However, such products are often misused against the labelling requirements in locations where these articles are not supposed to be used, like football stadiums and demonstrations. In contrast to the intended use, uninvolved third parties are likely exposed to these reaction products without proper protective equipment. This study aims at identifying the transient particle size range of the aerosols emitted during the functioning of such common smoke generators for simulation purposes. In total four different types of articles were investigated, with five colors per type (white, blue, green, red, orange). Results show that the majority of the particles were emitted in a range between 40 nm and 350 nm, with some variation depending on the smoke color. Particles with diameters of less than 100 nm are generally of specific concern, as they can penetrate the alveolar system of the human lungs and therefore present a specific hazard. KW - FMPS KW - Simulation devices PY - 2016 VL - 2016 IS - 35 SP - 3 EP - 8 PB - Journal of Pyrotechnics CY - Cambridgeshire (UK) AN - OPUS4-35742 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Schoknecht, Ute A1 - Mathies, Helena A1 - Wegner, R. A1 - Uhlig, S. A1 - Baldauf, H. A1 - Colson, B. T1 - Emissions of material preservatives into the environment - realistic extimation of environmental risks through the improved characterisation of the leaching of biocides from treated materials used outdoors N2 - This report supports the implementation of European regulations on biocidal products for the product types 7, 9 and 10. Emission of active substances from material preservatives into environmental compartments can occur due precipitation. Risk characterisations have to be based on estimations of environmental concentrations of target substances leached from material preservatives. Harmonised test procedures are required to predict environmental impact due to leaching. Seventeen treated articles, mainly paints, but also a textile, sealing tapes and sealing masses were investigated by intermittent contact to water to prove suitability of the proposed laboratory test procedure. Parameters that affect leaching of active substances were examined. Further development of a semi-analytical model to describe laboratory leaching data revealed that the model can be improved if changes of the leachability of substances during the test are supposed and integrated. Six paints and a textile were exposed to weathering to compare results from laboratory and field experiments. Similarities between leaching processes in both test approaches were observed. Generally, emissions of active substances are considerably higher in laboratory tests than from vertically installed test specimens exposed to weathering. Competing processes that cause losses of active substances can occur in both tests, but to a higher degree in field experiments. In addition, the influence of meteorological parameters on leaching processes was investigated. Factors besides the amount of driving rain were identified that effect leaching processes by complex interaction. Relations between laboratory and field data were analysed, and the applicability of the semi-analytical model was tested for field data. Guidance documents for leaching tests under laboratory and field conditions were drafted and discussed with experts on EU Level workshop 3./4. July 2014, BAM, Berlin), and are included in this Report. N2 - Dieser Bericht unterstützt die Umsetzung europäischer Regelungen für Biozidprodukte der Produkttypen 7, 9 und 10. Die Freisetzung von Wirkstoffen aus Materialschutzmitteln in Umweltkompartimente kann bei Niederschlagsereignissen auftreten. Risikocharakterisierungen müssen auf Abschätzungen von Umweltkonzentrationen der betrachteten Stoffe basieren. Harmonisierte Testverfahren sind erforderlich, um eine Beeinflussung der Umwelt durch Auswaschung vorherzusagen. Siebzehn behandelte Waren, vor allem Farben, aber auch ein Textil, Dichtungsbänder und Dichtungsmassen wurden bei periodischem Wasserkontakt untersucht, um die Anwendbarkeit des vorgeschlagenen Laborverfahrens nachzuweisen. Faktoren, die Einfluss auf die Auswaschung von Wirkstoffen haben, wurden untersucht. Die Wieterentwicklung eines semianalytischen Modells zur Beschreibung von Auswaschdaten aus Laborversuchen ergab, dass das Modell verbessert werden kann, wenn eine Änderung der Auswaschbarkeit von Substanzen während des Versuchs angenommen und einbezogen wird. Sechs Farben und ein Textil wurden der Witterung ausgesetzt, um Ergebnisse aus Labor- und Freilandversuchen zu vergleichen. Zwischen Auswaschprozessen in beiden Versuchsansätzen wurden Ähnlichkeiten beobachtet. Im Allgemeinen sind die Emissionen von Wirkstoffen im Laborversuch deutlich höher als bei vertikal installierten bewitterten Prüfkörpern. Konkurrierende Prozesse, die Verluste an Wirkstoffen bewirken, können in beiden Testverfahren auftreten, sind aber im Freilandversuch stärker ausgeprägt. Außerdem wurde der Einfluss von meteorologischen Parametern auf Auswaschprozesse untersucht. Es wurden Faktoren identifiziert, die zusätzlich zur Schlagregenmenge durch komplexe Interaktion auf Auswaschprozesse wirken. Beziehungen zwischen Labor- und Frei-landdaten wurden analysiert und die Anwendbarkeit des semianalytischen Modells für Freilanddaten getestet. Leitfäden für Auswaschversuche unter Labor-bzw. Freilandbedingungen wurden entworfen, mit europäischen Experten diskutiert (Workshop 3./4. Juli 2014, BAM, Berlin) und sind Bestandteil dieses Berichts. KW - EU Biocides Regulation KW - Leaching tests KW - Treated articles KW - Modelling KW - EU-Biozidverordnung KW - Auswaschversuche KW - Behandelte Waren KW - Modellierung PY - 2016 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-355139 SN - 1862-4804 SP - 1 EP - 136 AN - OPUS4-35513 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Holm, Olaf A1 - Rein, A. A1 - Trapp, S. A1 - Popp-Hoffmann, S. A1 - Bittens, M. A1 - Leven, C. A1 - Dietrich, P. T1 - Comparison of phytoscreening and direct-push-based site investigation at a rural megasite contaminated with chlorinated ethenes N2 - The reliable characterization of subsurface contamination of spatially extended contaminated sites is a challenging task, especially with an unknown history of land use. Conventional technologies often fail due to temporal and financial constraints and thus hinder the redevelopment of abandoned areas in particular. Here we compare two site screening techniques that can be applied quickly at relatively low cost, namely Direct Push (DP)-based groundwater sampling and tree core sampling. The effectiveness of both methods is compared for a rural megasite contaminated with chlorinated hydrocarbons. Unexpected pollution hot spots could be identified using both of these methods, while tree coring even enabled the delineation of the contaminant plume flowing into an adjacent wetland inaccessible for DP units. Both methods showed a good agreement in revealing the spatial pattern of the contamination. The correlation between groundwater concentrations and equivalent concentrations in wood was linear and highly significant for trichloroethene. Correlation was less obvious for its metabolite cis-dichloroethene, but still significant. As outcome of our study we recommend tree coring and for initial screening in combination with a DP sampling to retrieve quantitative data on groundwater pollutants in order to assess the contamination situation of a non- or only partly investigated site. The subsequent placement of monitoring wells for long-term monitoring of contamination levels is recommended. A combination of methods would achieve more relevant information at comparable or possibly even lower efforts in comparison to a conventional site investigation. KW - non-invasive investigation KW - phytoscreening KW - direct-push KW - screening KW - trichloroethene KW - trichloroethylene KW - groundwater contamination KW - tree core sampling PY - 2015 UR - http://onlinelibrary.wiley.com/doi/10.1111/gwmr.12122/abstract DO - https://doi.org/10.1111/gwmr.12122 SN - 1745-6592 VL - 35 IS - 4 SP - 45 EP - 56 PB - Wiley Online Library AN - OPUS4-35595 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Muhammad, R. A1 - Greiser, Sebastian A1 - Kosslick, H. A1 - Ibad, F. A1 - Jäger, Christian A1 - Rabeah, J. A1 - Schulz, A. T1 - Influence of the of dealumination and porosity on the acid sites of natural zeolite clinoptilolite N2 - Heavy feedstock from crude and bio oil is a widely available and renewable resource for production of fuel and starting materials for other organic valuables by cracking or hydrocracking. Catalytic processing of heavy feedstock can meet the increased demand of energy up to a great extent. It requires the application of acidic catalysts like zeolites. However, the used synthetic catalysts are difficult to recover and reuse and are mostly spent. The use of natural zeolite as spent catalysts may open new perspectives in the chemical use of heavy feed feedstock by chemical conversion. Natural zeolites are not expensive, widely available and environment friendly. Clinoptilolite is the most abundant natural zeolite. Clinoptilolite has a crystalline structure with a defined micropore system of medium size showing unique ion exchange and sorption properties. However, it is catalytically active only in the H-form. Also certain porosity is required for improvement of the accessibility of active sites. This paper deals with the tuning of acid properties and of the mesoporosity of the clinoptilolite by variation of the Si/Al framework ratio, extra-framework aluminum and modification of the porosity by specific acid and water vapor treatment. The preparation of hierarchical pore structures containing interconnected micro-meso-macropores is an important factor influencing the catalytic performance. The obtained materials have been characterized by XRD, TEM, FTIR, Raman, TG/DSC. The chemical composition has been determined by ICP-AES. The porosity have been investigated by nitrogen adsorption desorption measurements. The acidity has been measured by Ammonia-TPD. The extent of dealumination, stability of the clinoptilolite against acid treatment and the change in the nature of acid sites and their local structure has been studied by solid state 29Si and 27Al MAS NMR spectroscopy in detail. The catalytic activity has been investigated in the acetalization of benzaldehyde with 1,3-butanediol. The impact of the porosity, change of the Si/Al ratio as well as present Al species on catalytic properties will be discussed. T2 - 28. Deutsche Zeolith-Tagung CY - Giessen, Germany DA - 02.03.2016 KW - NMR KW - Zeolite KW - Clinoptilolite KW - Catalyst PY - 2016 AN - OPUS4-35886 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Greiser, Sebastian A1 - Sturm, Patrick A1 - Gluth, Gregor A1 - Hunger, M. A1 - Jäger, Christian T1 - 29Si-27Al NMR to verify and distinguish Qn(mAl) sites in zeolites and geopolymers N2 - One-part-geopolymers, produced by addition of water to a mixture of solid silica and sodium alumi-nate, are a less exhaustively studied approach to form geopolymeric binders. Depending on the silica source, the reaction products show significant amounts of zeolite Na-A besides amorphous compounds. Previously, 29Si MAS NMR has been used to analyze the chemical structure of such one-part geopolymers, having crystalline structures and amorphous phases (Q2, Q3, Q4). In this work, pure zeolites and three different one-part-geopolymers cured for 1 day were investigated by 29Si-27Al TRAPDOR NMR. It was used to identify aluminum phases in overlapping silicon sites. Zeolites Na-X (Si/Al=1.4) and Na-Y (Si/Al=2.7) served as model systems to measure the TRAPDOR effect of the structural units Q4(mAl). Both materials show several Q4(mAl) signals, which are all separated by their chemical shifts. The more aluminum surrounds the silicon tetrahedron the higher are the normalized TRAPDOR difference signals (S0/∆S). The intensity ratios between Q4(mAl) to Q4({m-1}Al) of these signals is fixed but vary slightly between both zeolites. These results are transferred to the complex geopolymer structure. T2 - 57th Experimental nuclear magnetic resonance conference CY - Pittsburgh, PA, USA DA - 10.04.2016 KW - NMR KW - 29Si-27Al TRAPDOR MAS KW - Geopolymer KW - One-part formulation KW - Rice husk ash PY - 2016 AN - OPUS4-35864 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kalbe, Ute A1 - Krüger, Oliver A1 - Bandow, Nicole A1 - Geburtig, Anja ED - Reichert, Thomas T1 - Laboratory Weathering Test for Simulating the Leaching from Artificial Sporting Grounds N2 - In order to estimating the amount and kind of emissions from polymeric sporting tracks a laboratory test was developed in BAM that combines artificial weathering and subsequent batch and column tests [1, 2, 3]. The goal consisted in nvestigating a possible change of emissions into soil and groundwater of a new sporting ground as a function of ageing and egradation of the system. At various stages of the weathering, the samples were removed and subjected to especially designed column extraction tests for the solid samples. Both these extracts and the accumulated rainwater run-off of the weathering device were subjected to analytical tests of their ingredients. The overall aim of the test consisted in developing ontrollable, reproducible, and standardisable conditions and methods of investigation that would allow an estimation of the emissions in the course of outdoor application of about five years. T2 - 7th European Weathering Symposium. Natural and Artificial Ageing of Polymers CY - Naples, Italy DA - 16.09.2015 KW - artificial weathering KW - emission KW - leaching KW - sporting track KW - degradation KW - ageing KW - rubber KW - extraction PY - 2015 SN - 978-3-9816286-6-1 VL - CEES 17 SP - 195 EP - 208 PB - Thomas Würtz CY - Karlsruhe, Germany AN - OPUS4-35401 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Keller, Julia A1 - Haase, H. A1 - Koch, Matthias T1 - Electrochemistry coupled with mass spectrometry - a versatile tool to investigate metabolic processes of mycotoxins N2 - To understand the metabolic fate of food relevant mycotoxins in vitro systems were mainly used as the method of choice, so far. Yet, in recent years coupling of electrochemistry mass spectrometry (EC-MS) gained increasing importance as promising technique for fast simulation of metabolic processes and was successfully applied in particular for drug metabolism [1]. The aim of our work was to investigate the potential of EC-MS to predict phase I metabolites of priority mycotoxins and to compare the results with in vitro experiments. Hence, the EU-regulated Fusarium mycotoxins zearalenone (ZEN) and patulin as well as dihydroergocristine (DHEC) as model compound of ergot alkaloids were electrochemically oxidized and analyzed by EC MS for the first time. Electrochemical conditions were set-up individually for each of the three mycotoxins. By using a coulometric flow through cell with a diamond working electrode oxidation of the chosen mycotoxins was observed after applying potentials between 1.7 and 2.0 V vs. Pd/H2. The electrochemically generated reaction products were analyzed online by mass-spectrometric detection. All of the three chosen mycotoxins were electrochemically converted to mono- and/or dihydroxylated products confirming the results of ZEN related metabolism studies [2, 3] and in case of DHEC own results from in vitro assays. Due to a lack of metabolism studies concerning the oxidative fate of patulin, interpretation of EC-MS data and performing microsomal studies is of particular relevance. Beside the identified products from electrochemical oxidation of ZEN, patulin and DHEC there is still a number of yet unknown compounds. Additional structural characterization of detected compounds by NMR and X-ray analysis will be facilitated by their large-scale production using preparative EC cells. T2 - Mycotoxin Workshop CY - Berlin, Germany DA - 02.05.2016 KW - Mycotoxins KW - Electrochemistry KW - Biotransformation KW - Mass spectrometry PY - 2016 AN - OPUS4-35981 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Climent Terol, Estela A1 - Biyikal, Mustafa A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Rapid, sensitive and selective fluorescence sensing device for the determination of the nerve agents Sarin, Soman and Tabun in the gas phase N2 - The organophosphate nerve agents Tabun (GA), Sarin (GB) and Soman (GD) are among the most toxic chemical warfare agents (CWA) known, and exert their biological effects by irreversibly inhibiting acetylcholinesterase enzymes of the human nerve system. The use of these agents in the past century has killed millions of civilians around the world during World Wars I and II, and after that, approximately 40.000 tons of chemical ammunition were dumped into the Baltic Sea, containing ca. 13.000 tons of chemical warfare agents. Since their production, almost all the nations of the world have been strictly avoiding the development and use of CWA, participating in active destruction of CWA stockpiles, especially since the Chemical Weapon Convention (CWC) of 1993. However, CWA have been used in offensive ways against civilian population by terrorists, as for instance in the fatal Tokyo subway terror incident of 1995 or, most recently, against antigovernment demonstrators in Syria in 2013. Due to their higher toxicity and continuous use, it is therefore very important to develop simple and fast detection methods relying on new nerve agent sensing modalities for use in control and inspection. A practically useful fluorescent probe must possess a rapid response and high sensitivity, and shall be implementable into easy-to-use devices for real time detection by untrained personnel. Taking into account this fact, in this work we have synthesized several mesoporous silica materials containing boron–dipyrromethene (BODIPY) moieties for the detection of nerve agents GA, GB and GD in the gas phase. Development of our system indicated that the most potent materials are able to respond to the presence of nerve agent simulants diethyl cyanophosphonate, diethyl chlorophosphate and diisopropyl fluorophosphate, which have to be used in a laboratory setting, yet also for the real nerve agents Sarin, Soman and Tabun, producing a strong quenching of the fluorescence. Furthermore, a portable device for the detection of GA, GB and GD in the gas phase has been prepared for in situ sensing and rapid screening applications, consisting of strips that are able to indicate the targets down to below 1 mgm-3 which is below the LD50 values. T2 - XIII Europtrode Conference CY - Graz, Austria DA - 20.03.2016 KW - nerve agent KW - mesoporous material KW - sensor PY - 2016 AN - OPUS4-35921 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gorbushina, Anna T1 - Life on the rocks and other hard surfaces N2 - After their discovery on rock surfaces in cold and hot deserts, a polyphyletic group of ascomycetous black fungi was found to dominate a range of hostile environments – natural and man-made, from salterns to dishwashers, roofs and solar panels. Together with bacteria and algae they may establish subaerial biofilms and cause weathering of the surfaces they grow on. Their impressive survival abilities as well as their constitutive protective pigmentation and cluster-like microcolony organisation are similar in environmental isolates as well as in heat-tolerant opportunistic pathogens of animals and humans. We selected the rock-inhabiting fungus Knufia petricola (class Eurotiomycetes, order Chaetothyriales) that exhibits all the characteristics of microcolonial black fungi such as yeast-like cell growth, absence of reproductive structures and constitutive dihydroxynaphthalene (DHN) melanogenesis (Nai et al. 2013, Fungal Genet Biol). We developed protocols to efficiently generate and transform protoplasts resulting in stable homokaryotic transformants by targeting genes involved in pigment synthesis. The differences between the wild-type fungus and its melanin-deficient mutants were tested in geomicrobiological experiments and measured are now discussed in detail, with special accent on the possible effects of the mutation on EPS and other exuded substances. T2 - Departmental colloquium of the Tuscia University CY - Viterbo, Italy DA - 14.02.2020 KW - Ascomycetes KW - Fungal biofilms KW - Material surface colonisation KW - Genetics KW - Genomics KW - Extremophilic fungi PY - 2020 AN - OPUS4-50643 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Meermann, Björn A1 - Von der Au, Marcus A1 - Borovinskaya, O. A1 - Büchel, C. T1 - Metal based pollutant assessment via diatoms - new possibilities via automated single cell-ICP-ToF-MS N2 - Diatoms are located at the bottom of the food chain. Toxicological relevant metals can possibly accumulate within the food web and cause harmful effects. Diatoms are a test system in ecotoxicology. Toxicological effects weaken the growth of algae which is by default investigated by means of fluorescence detection. On basis of the expose concentration as well as obtained fluorescence data potential threshold exceedance in e.g. surface waters is assessed. However, this approach does not allow for the determination of “real” accumulated metal concentration in diatoms. Common approaches are based on bulk analysis via e.g. ICP-MS, ICP-OES or AAS. But, biological variability is completely disregarded. To tackle this problem, alternative approaches are highly needed. Within the last years, sp-ICP-MS for nanoparticle as well as single cell analysis turned out as a powerful technique to analyze metal contents as well as size distributions on broad size range. But, common ICP-MS systems do not allow for multi-element detection within single particle/cell events. Thus, simultaneous MS detection devices are needed - just recently, ICP-ToF-MS experienced a revival. Within our previous work, we developed an automated sample introduction system based on a HPLC system on-line with single particle-ICP-MS, which allowed for ionic background separation and single algae analysis. However, for unambiguous tracing several fingerprint elements and multielement analysis in single algae (diatoms) is needed. Thus, we coupled our previous setup on-line to ICP-ToF-MS. Test diatom species were exposed to test substances (Zn) as well as nanoparticles (FeNPs). The developed setup allowed for a fast, automated and multielement analysis in single diatoms. Furthermore, we combined our approach with multivariate data assessment - multielement detection of characteristic fingerprint elements allowed for an unambiguous diatom tracing. Clustering of diatoms according to metal exposure concentration levels was enabled. Our approach is a new potential tool in ecotoxicological testing. T2 - 53. DGMS & 27th ICP-MS user meeting CY - Münster, Germany DA - 01.03.2020 KW - Single cell-ICP-ToF-MS KW - Diatoms KW - New ecotoxicological testing tools PY - 2020 AN - OPUS4-50644 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Priebe, Nsesheye Susan A1 - Sturm, Patrick A1 - Kühne, Hans-Carsten A1 - Gluth, Gregor ED - Bishnoi, S. T1 - Comparison of brick clays and a kaolinitic clay regarding calcination and performance in blended cement mortars N2 - Two brick clays (rich in 2:1 clay minerals) and a low-grade kaolinitic clay were studied regarding their transformations during calcination and their performance in blended cement mortars. The mortars with calcined clays exhibited decreased workability (slump flow), but this effect could be mitigated by employment of a conventional superplasticizer; however, compressive strength of the hardened mortar was lowered in some cases. While the kaolinitic clay generally yielded the highest strength, the performance of a brick clay could be increased by grinding to higher fineness and by mixing it with the kaolinitic clay. T2 - 3rd International Conference on Calcined Clays for Sustainable Concrete CY - New Delhi, India DA - 15.10.2019 KW - Calcined clays KW - Blended cements KW - Workability KW - Supplementary cementitious materials PY - 2020 SN - 978-981-15-2805-7 DO - https://doi.org/10.1007/978-981-15-2806-4_10 N1 - Geburtsname von Priebe, Nsesheye Susan: Msinjili, N. S. - Birth name of Priebe, Nsesheye Susan: Msinjili, N. S. VL - 25 SP - 85 EP - 93 PB - Springer CY - Singapore AN - OPUS4-50663 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -