TY - JOUR A1 - Rietsch, P. A1 - Witte, F. A1 - Sobottka, S. A1 - Germer, G. A1 - Becker, A. A1 - Güttler, Arne A1 - Sarkar, B. A1 - Paulus, B. A1 - Resch-Genger, Ute A1 - Eigler, S. T1 - Diaminodicyanoquinones: Fluorescent dyes with high dipole moments and electron-acceptor properties JF - Angewandte Chemie Int. Ed. N2 - Fluorescent dyes are applied in various fields of research,includingsolarcellsandlight-emittingdevices,andas reporters for assays and bioimaging studies.Fluorescent dyes with an added high dipole moment pave the way to nonlinear optics and polarity sensitivity.Redox activity makes it possible to switch the moleculeQsphotophysical properties.Diaminodicyanoquinone derivatives possess high dipole moments,yet only lowfluorescence quantum yields,and have therefore been neglected as fluorescent dyes.Here we investigate the fluorescencepropertiesofdiaminodicyanoquinonesusingacombined theoretical and experimental approach and derive molecules with afluorescence quantum yield exceeding 90%. The diaminodicyanoquinone core moiety provides chemical versatility and can be integrated into novel molecular architectures with unique photophysical features. KW - Dipole moment KW - Fluorescence KW - Quantum yield KW - Quinones PY - 2019 DO - https://doi.org/10.1002/anie.201903204 SN - 1433-7851 SN - 1521-3773 VL - 58 IS - 24 SP - 8235 EP - 8239 PB - Wiley Online Libary CY - Weihnheim AN - OPUS4-48890 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Resch-Genger, Ute A1 - Brunet, G. A1 - Marin, R. A1 - Monk, Melissa-Jane A1 - Galico, D. A. A1 - Sigoli, F. A. A1 - Suturina, E. A. A1 - Hemmer, E. A1 - Murugesu, M. T1 - Exploring the dual functionality of an Ytterbium complex for luminescence thermometry and slow magnetic relaxation† JF - Chemical Science N2 - We present a comprehensive investigation of the magnetic and optical properties of an ytterbium complex, which combines two desirable and practical features into a single molecular system. Based upon YbIII Ions that promote near-infrared optical activity and a chemical backbone that is ideal for an in-depth understanding of the magnetic behaviour, we have designed a multifunctional opto-magnetic species that operates as a luminescent thermometer and as a single-molecule magnet (SMM). Our magnetic investigations, in conjunction with ab initio calculations, reveal one of the highest energy barriers reported for an YbIII-based complex. Moreover, we correlate this anisotropic barrier with the Emission spectrum of the compound, wherein we provide a complete assignment of the energetic profile of the complex. Such studies lay the foundation for the design of exciting multi-faceted materials that are able to retain information at the single-molecule level and possess built-in thermal self-monitoring capabilities. KW - Magnetic KW - Fluorescence KW - NIR KW - Temperature KW - Dual sensing KW - Sensor KW - Yb(III) complex KW - Lanthanide KW - Quantum yield KW - Quality assurance PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-486659 DO - https://doi.org/10.1039/c9sc00343f VL - 10 IS - 28 SP - 6799 EP - 6808 PB - Royal Society of Chemistry AN - OPUS4-48665 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Kage, Daniel A1 - Weigert, Florian A1 - Martynenko, Irina A1 - Dhamo, Lorena A1 - Soares, J. X. T1 - Luminescent nanocrystals – Photophysics and applications for lifetime multiplexing N2 - Bioanalytical, diagnostic, and security applications require the fast and sensitive determination of a steadily increasing number of analytes or events in parallel in a broad variety of detection formats.[1,2] Ideal candidates for spectral encoding and multiplexing schemes are luminescent nanocrystals like semiconductor quantum dots (QDs), particularly Cd-containing II/VI QDs with their narrow and symmetric emission bands. With the availability of relatively simple and inexpensive instrumentation for time-resolved fluorescence measurements, similar strategies utilizing the compound-specific parameter fluorescence lifetime or fluorescence decay kinetics become increasingly attractive.[3-5] The potential of different types of QDs like II/VI, III/V and Cd-free ternary QDs such as AgInS (AIS) QDs for lifetime-based encoding and multiplexing has been, however, barely utilized, although the lifetimes of these nanocrystals cover a time windows which is barely accessible with other fluorophores. Here we present a brief insight into the photophysics of AIS QDs and show the potential of dye- and QD-encoded beads for lifetime-based encoding and detection schemes in conjunction with flow cytometry and fluorescence lifetime imaging microscopy T2 - Nanax 2019 CY - Hamburg, Germany DA - 16.09.2019 KW - Nano KW - Microparticle KW - Bead KW - Encoding KW - Lifetime KW - Multiplexing KW - Flow cytometry KW - Bead-based assay KW - Fluorescence KW - Dye KW - LT-FCM KW - Time-resolved flow cytometry KW - Method PY - 2019 AN - OPUS4-49039 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Advanced characterization of nanomaterials N2 - The rational synthesis and use of nanomaterials require the characterization of many different properties, ranging from particle size and size distribution over surface chemistry to more applicationrelevant features like optical, electrochemical, and magnetic properties. In the following, several methods for the characterization of functional groups on nanomaterials, like polymer and silica nanoparticles, semiconductor quantum dots, and lanthanide-based upconversion nanocrystals are presented. Additionally, procedures for the measurement of the key spectroscopic performance parameters of nanomaterials with linear and nonlinear photoluminescence, such as the photoluminescence quantum yield, are presented for the UV/vis/NIR/SWIR. T2 - Summerschool CY - Bad Honnef, Germany DA - 22.07.2019 KW - Quantum yield KW - Nanoparticle KW - Fluorescence KW - Quantum dot KW - NIR KW - SWIR KW - Quality assurance KW - Calibration PY - 2019 AN - OPUS4-48630 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta A1 - Würth, Christian A1 - Güttler, Arne A1 - Richter, Maria A1 - Schneider, Thomas T1 - Determining Photoluminescence Quantum Yields of Molecular and Nanocrystal Emitters in the UV/vis/NIR/SWIR N2 - The comparison of different emitter classes and the rational design of the next generation of molecular and nanoscale reporters require accurate and quantitative photo-luminescence measurements. This is of special importance for all photoluminescence applications in the life and material sciences and nanobiophotonics. In the following, procedures for the determination of the spectroscopic key parameter photoluminescence quantum yield, i.e., the number of emitted per absorbed photons, in the UV/vis/NIR/SWIR are presented including pitfalls and achievable uncertainties and material-specific effects related to certain emitter classes are addressed. T2 - Kolloqium National Center for Nanoscience and Technology CY - Peking, People's Republic of China DA - 18.10.2019 KW - Fluorescence KW - Quantum yield KW - Integrating sphere spectroscopy KW - Dye KW - Nanocrystal KW - NIR KW - SWIR KW - Quantum dot KW - Fluorescence standard KW - Uncertainty KW - Calibration PY - 2019 AN - OPUS4-49362 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Weigert, Florian A1 - Frenzel, Florian A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Martynenko, Irena A1 - Dhamo, Lorena T1 - Photoluminescence Properties of Different Types of Nanocrystals at the Ensemble and Single Emitter Level N2 - Correlating the photoluminescence (PL) properties of nanomaterials like semiconductor nanocrystals (QDs) and upconversion nanocrystals (UCNPs) assessed in ensemble studies and at the single particle level is increasingly relevant for applications of these nanomaterials in the life sciences like bioimaging studies or their use as reporters in microfluidic assays. Here we present a comparison of the spectroscopic properties of ensembles and single emitters for QDs like II/VI QDs and cadmium-free AIS/ZnS QDs as well as different UCNPs. The overall goal of this study was to derive particle architectures well suited for spectroscopic and microscopic applications. T2 - BIOSSPIE CY - San Francisco, CA, USA DA - 02.02.2019 KW - Quantum yield KW - Nanomaterial KW - Photoluminescence KW - Absolute fluorometry KW - Integrating sphere spectroscopy, KW - NIR KW - IR KW - Fluorescence KW - Nanoparticle KW - Semiconductor KW - Quantum dot KW - Single particle spectroscopy KW - Surface chemistry PY - 2019 AN - OPUS4-47358 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Kaiser, Martin A1 - Pauli, Jutta A1 - Hoffmann, Katrin T1 - Relative and Absolute Methods for Measuring Photoluminescence Quantum Yields of UV/vis/NIR Emitters N2 - One of the key spectroscopic performance parameters of molecular and particulate emitters is the photoluminescence quantum yield (PL QY) that provides a direct measure for the number of emitted per absorbed photons. This triggered the interest in methods suitable for measuring this property for emitters in various environments in the UV/vis/NIR and above 1000 nm as well as on the ensemble and single emitter level. Moreover, for nonlinear emitters like lanthanide-based upconversion nanocrystals methods including instrumentation for power density-dependent PL QY studies are required. An overview of the research activities in Division Biophotonics of BAM is given and suitable relative and absolute methods for the deter-mination of PL QY of organic dyes and different types of application-relevant nanomaterials in dispersion and in the solid state are presen-ted. This covers also the design and calibration of integrating sphere setups, achievable uncertainties, and candidates for PL QY reference materials. T2 - OSRAM Veranstaltung CY - Regensburg, Germany DA - 09.01.2019 KW - Quantum yield KW - Calibration KW - Reference material KW - Uncertainty KW - Absolute fluorometry KW - Integrating sphere spectroscopy KW - NIR KW - IR KW - Fluorescence KW - Nanoparticle KW - Dye PY - 2019 AN - OPUS4-47263 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ren, J. A1 - Weigert, Florian A1 - Weber, F. A1 - Wang, Y. A1 - Choudhury, S. A1 - Xiao, J. A1 - Lauermann, I. A1 - Resch-Genger, Ute A1 - Bande, A. A1 - Petit, T. ED - Petit, Tristan T1 - Influence of surface chemistry on optical, chemical and electronic properties of blue luminescent carbon dots JF - Nanoscale N2 - Carbon dots have attracted much attention due to their unique optical, chemical and electronic properties enabling a wide range of applications. The properties of carbon dots can be effectively adjusted through modifying their chemical composition. However, a major challenge remains in understanding the core and surface contributions to optical and electronic transitions. Here, three blue luminescent carbon dots with carboxyl, amino and hydroxyl groups were comprehensively characterized by UV-vis absorption and emission spectroscopy, synchrotron-based X-ray spectroscopy, and infrared spectroscopy. The influence of the surface functionality on their fluorescence was probed by pH-dependent photoluminescence measurements. Moreover, the hydrogen bonding interactions between water and the surface groups of carbon dots were characterized by infrared spectroscopy. Our results show that both core and surface electronic states of blue luminescent carbon dots contribute to electronic acceptor levels while the chemical nature of the surface groups determines the hydrogen bonding behavior of the carbon dots. This comprehensive spectroscopic study demonstrates that the surface chemistry has a profound influence on the electronic configuration and surface–water interaction of carbon dots, thus affecting their photoluminescence properties. KW - Quantum dots KW - Spectroscopy KW - UV Vis KW - Fluorescence KW - Surface chemistry PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-472325 DO - https://doi.org/10.1039/c8nr08595a SN - 2040-3372 VL - 11 IS - 4 SP - 2056 EP - 2064 PB - RSC AN - OPUS4-47232 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Radunz, Sebastian A1 - Wedepohl, S. A1 - Röhr, Mathilde A1 - Calderón, M. A1 - Tschiche, H. R. A1 - Resch-Genger, Ute T1 - pH-Activatable Singlet Oxygen-Generating Boron-dipyrromethenes JF - Journal of Medicinal Chemistry N2 - Singlet oxygen can severely damage biological tissue, which is exploited in photodynamic therapy (PDT). In PDT, the effective range is limited by the distribution of the photosensitizer (PS) and the illuminated area. However, no distinction is made between healthy and pathological tissue, which can cause undesired damage. This encouraged us to exploit the more acidic pH of cancerous tissue and design pH-controllable singlet oxygen-generating boron-dipyrromethene (BODIPY) dyes. A pH sensitivity of the dyes is achieved by the introduction of an electronically decoupled, photoinduced electron transfer (PET)-capable subunit in meso-position of the BODIPY core. To favor triplet-state formation as required for singlet Oxygen generation, iodine substituents were introduced at the chromophore core. The resulting pH-controlled singlet oxygen-generating dyes with pKa values in the physiological range were subsequently assessed regarding their potential as pH-controlled PS for PDT. Using HeLa cells, we could successfully demonstrate markedly different pH-dependent cytotoxicities upon illumination. KW - Fluorescence KW - Sensor KW - Switch KW - pH KW - Singlet oxygen KW - PDT KW - Cell KW - BODIPY KW - Dye KW - Probe KW - Synthesis PY - 2019 DO - https://doi.org/10.1021/acs.jmedchem.9b01873 VL - 63 IS - 4 SP - 1699 EP - 1708 PB - ACS Publications AN - OPUS4-50554 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Meierhofer, F. A1 - Dissinger, F. A1 - Weigert, Florian A1 - Resch-Genger, Ute A1 - Waldvogel, S. R. A1 - Voss, T. T1 - Citric-Acid-Based Carbon Dots with Luminescence Quantum Yields > 50%: spectral tuning of the luminescence by ligand exchange and pH adjustment N2 - We report the synthesis and characterization of carbon nanodots (CDs) with high quantum yield (>50%) and tailored optical absorption as well as emission properties. A well-described protocol with polyethyleneimine (PEI) as amine precursor is used as a reference to a new CD system which is stabilized by aromatic 2,3-diaminopyridine (DAP) molecules instead. The DAP stabilizer is installed in order to red-shift the absorption peak of the n-π* electron transition allowing efficient radiative recombination and light emission. Size, shape, and chemical composition of the samples are determined by (HR)TEM, EDX and FTIR-spectroscopy. Optical parameters are investigated using UV-VIS, PL and QY measurements. Several parameters such as concentration, excitation wavelength and pH are studied. Zeta-potential analysis indicate that pH-induced (de-)protonation processes of functional moieties directly affect the n-π* energy bands. This results in unique pH-dependent absorption and emission characteristics which are discussed on the specific chemical composition of each CD system. T2 - MRS 2019 CY - Boston, MA, USA DA - 03.12.2019 KW - Nanoparticle KW - Carbon dot KW - Surface chemistry KW - Fluorescence KW - PH KW - Ligand KW - FTIR KW - Synthesis KW - Characterization PY - 2019 AN - OPUS4-49968 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Martynenko, Irina V. A1 - Kusic, Dragana A1 - Weigert, Florian A1 - Stafford, S. A1 - Donnelly, F. C. A1 - Evstigneev, R. A1 - Gromova, Y. A1 - Baranov, A. V. A1 - Rühl, Bastian A1 - Kunte, Hans-Jörg A1 - Gun'ko, Y. K. A1 - Resch-Genger, Ute T1 - Magneto-fluorescent microbeads for bacteria detection constructed from superparamagnetic Fe3O4 nanoparticles and AIS/ZnS quantum dots JF - Analytical chemistry N2 - The efficient and sensitive detection of pathogenic microorganisms in aqueous environments, such as water used in medical applications, drinking water, and cooling water of industrial plants, requires simple and fast methods suitable for multiplexed detection such as flow cytometry (FCM) with optically encoded carrier beads. For this purpose, we combine fluorescent Cd-free Ag−In−S ternary quantum dots (t-QDs) with fluorescence Lifetimes (LTs) of several hundred nanoseconds and superparamagnetic Fe3O4 nanoparticles (SPIONs) with mesoporous CaCO3 microbeads to a magneto-fluorescent bead platform that can be surface-functionalized with bioligands, such as antibodies. This inorganic bead platform enables immuno-magnetic separation, target enrichment, and target quantification with optical readout. The beads can be detected with steady-state and time-resolved fluorescence microscopy and flow cytometry (FCM). Moreover, they are suited for readout by time gated emission. In the following, the preparation of these magneto-fluorescent CaCO3 beads, their spectroscopic and analytic characterization, and their conjugation with bacteria-specific antibodies are presented as well as proof-of-concept measurements with Legionella pneumophila including cell cultivation and plating experiments for bacteria quantification. Additionally, the possibility to discriminate between the long-lived emission of the LT-encoded capture and carrier CaCO3 beads and the short-lived Emission of the dye-stained bacteria with time-resolved fluorescence techniques and single wavelength excitation is demonstrated. KW - Fluorescence KW - method KW - lifetime KW - quantum yield KW - particle KW - magnetic nanoparticle KW - immunoseparation KW - flow cytometry KW - fluorescence microscopy KW - nanoparticle KW - quantum dot KW - AIS QD KW - fluorescence KW - bacteria detection KW - bacteria KW - antibody KW - Legionella KW - screening tes KW - FLIM PY - 2019 DO - https://doi.org/10.1021/acs.analchem.9b01812 SN - 0003-2700 SN - 1520-6882 VL - 91 SP - 12661 EP - 12669 PB - American Chemical Society CY - Washington, DC AN - OPUS4-50117 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hübner, Oskar A1 - Resch-Genger, Ute T1 - Spectral and Lifetime Encoding of Polymer Particles with Cd-free Ternary Semiconductor Nanocrystals for Flow Cytometry with Time Resolved Fluorescence Detection N2 - Multiplexed encoding schemes of nano- and micrometer sized particles with fluorescent dyes or quantum dots (QDs) and their optical detection, are of increasing interest for applications in the life sciences, for example in flow cytometry. Almost all strategies utilizing fluorescence focus on spectrally distinguishable emission bands or colors and different intensity levels as fluorescence codes. The fluorescence parameter lifetime has been, however, barely exploited. In this work the goal is to perform multiplexing with encoding fluorophores with different fluorescence lifetimes (LTs). In comparison to the spectral multiplexing strategies this has the advantage, that the different fluorescence LT codes can be measured with the excitation and emission wavelength, thus reducing instrument costs. Moreover, LTs should not depend on emitter concentration. Unlike organic dyes, the LTs of which are typically < 10 ns, the fluorescence LTs of ternary semiconductor QDs that represent a “green” alternative to conventional Cd-containing QDs are in the range of several hundred ns, independent of oxygen concentration, and can be tuned to a certain extent by chemical composition and surface chemistry. This present a time region that can be barely covered by other emitters that have either much shorter or longer lifetimes. In this project, different encoding strategies will be assessed and the encoded particles will be then used for fluorescence assays for the analysis of several targets in parallel. Therefor the encoded particles will be functionalized with different target-specific bioligands and read out with a specifically designed flow cytometer enabling time-resolved fluorescence detection. With this instrument, the particles will be discriminated by their fluorescence LTs In one detection channel while the analytes will be quantified by fluorescence labels in a second channel in the intensity domain. T2 - Bad Honnef Physics School Exciting nanostructures: Characterizing advanced confined systems CY - Bad Honnef, Germany DA - 21.07.2019 KW - Multiplexing KW - Lifetime KW - Bead KW - Flow cytometry KW - Fluorescence KW - Quantum dot KW - InP KW - AIS KW - Dye KW - Encoding KW - Barcoding KW - Assay KW - Method PY - 2019 AN - OPUS4-49723 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hübner, Oskar A1 - Resch-Genger, Ute A1 - Wegner, Karl David A1 - Hoffmann, Kristin T1 - Lifetime Barcoding of Polystyrene Beads with Fluorescent Nanocrystals for Fluorescent Lifetime Detection in Flow Cytometry N2 - Multiplexed encoding schemes of nano- and micrometer sized polymer particles with fluorescent dyes or quantum dots (QDs) and their optical detection, are of increasing interest for applications in the life sciences, for example in flow cytometry. Almost all strategies utilizing fluorescence focus on spectrally distinguishable emission bands or colors and different intensity levels as fluorescence codes. In this work the goal is to perform multiplexing with encoding fluorophores with different fluorescence lifetimes (LTs). In comparison to the spectral multiplexing strategies this has the advantage, that the different fluorescence LT codes can be measured with the same excitation and emission wavelength, thus reducing instrumental costs. Moreover, LTs should not depend on emitter concentration. Unlike organic dyes, the LTs of which are typically <10ns, the fluorescence LTs of ternary semiconductor QDs which represent a “green” alternative to conventional Cd-containing QDs are in the range of several hundred ns, independent of oxygen concentration, and can be tuned to a certain extent by chemical composition and surface chemistry. This presents a time region that can barely be covered by other emitters that have either much shorter or longer lifetimes. In this project, different encoding strategies will be assessed, and the encoded particles will then be used for fluorescence assays for the analysis of several targets in parallel. Therefore, the encoded particles will be functionalized with different target-specific bioligands and read out with a specifically designed flow cytometer enabling time-resolved fluorescence detection. With this instrument, the particles will be discriminated by their fluorescence LTs in one detection channel while the analytes will be quantified by fluorescence labels in a second channel in the intensity domain. T2 - Tag der Chemie 2019 CY - Berlin, Germany DA - 11.07.2019 KW - Multiplexing KW - Lifetime KW - Bead KW - Flow cytometry KW - Fluorescence KW - Quantum dot KW - InP KW - AIS KW - Dye KW - Encoding KW - Barcoding KW - Assay KW - Method PY - 2019 AN - OPUS4-49722 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hübner, Oskar A1 - Kage, Daniel A1 - Hoffmann, Katrin A1 - Wegner, Karl David A1 - Dhamo, Lorena A1 - Göhde, W. A1 - Resch-Genger, Ute T1 - Luminescence lifetime encoding for flow cytometry with quantum-dot-encoded beads N2 - Spectral encoding of cells or particles and the discrimination of multiple spectral codes are a critical process in flow cytometry (FCM). Typical issues in spectral encoding are, e.g., the spectral overlap of codes, or the increasing complexity of instruments . The exploitation of the photoluminescence lifetime (LT) as an encoding parameter could be used to circumvent both of these issues, as it adds another dimension to the parameter space, or, when used as a stand-alone parameter, requiring only one excitation light source and one detector. While LT encoding was considered already decades ago it is still not implemented as a routine technique in FCM yet, mainly due to the challenge of very few photons being available within the limited transition time of a cell or particle through the laser spot. Recently, we demonstrated LT-FCM based on luminophores with ns LTs in a compact and low-cost flow cytometer. Measurements on polymer microbeads containing luminophores with distinctly different excited state LTs enabled the complete discrimination of three LT codes and five codes in total could be identified. Now, we have extended our approach towards considerably longer LTs by custom-made polymer microbeads loaded with different ratios of InP/ZnS and AgInS2 quantum dots. The use of these materials significantly expands the usable time range for LT encoding to up to several hundred ns. Our studies demonstrate the possibility to further increase the number of viable LT codes for multiplexing in LT-FCM without the need for extensive hardware modifications. T2 - Visions in Cytometry - 29th Annual Conference of the German Society for Cytometry CY - Berlin, Germany DA - 25.07.2019 KW - Multiplexing KW - Lifetime KW - Bead KW - Flow cytometry KW - Fluorescence KW - Quantum dot KW - InP KW - AIS KW - Dye KW - Encoding KW - Barcoding KW - Assay KW - Method PY - 2019 AN - OPUS4-49390 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Huang, J. A1 - Tarábek, J. A1 - Kulkarni, R. A1 - Wang, Cui A1 - Dračínský, M. A1 - Smales, Glen Jacob A1 - Pauw, Brian Richard A1 - Resch-Genger, Ute A1 - Bojdys, M. J. T1 - A π-conjugated, covalent phosphinine framework JF - Chemistry a European Journal N2 - Structural modularity of polymer frameworks is a key advantage of covalent organic polymers, however, only C, N, O, Si and S have found their way into their building blocks so far. Here, we expand the toolbox available to polymer and materials chemists by one additional nonmetal, phosphorus. Starting with a building block that contains a λ⁵‐phosphinine (C₅P) moiety, we evaluate a number of polymerisation protocols, finally obtaining a π‐conjugated, covalent phosphinine‐based framework (CPF‐1) via Suzuki‐Miyaura coupling. CPF‐1 is a weakly porous polymer glass (72.4 m2 g‐1 N2 BET at 77 K) with green fluorescence (λmax 546 nm) and extremely high thermal stability. The polymer catalyzes hydrogen evolution from water under UV and visible light irradiation without the need for additional co‐catalyst at a rate of 33.3 μmol h‐¹ g‐¹. Our results demonstrate for the first time the incorporation of the phosphinine motif into a complex polymer framework. Phosphinine‐based frameworks show promising electronic and optical properties that might spark future interest in their applications in light‐emitting devices and heterogeneous catalysis. KW - Phosphinine KW - Fully aromatic frameworks KW - Suzuki-Miyaura coupling KW - Polymers KW - Fluorescence KW - Small-angle scattering PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-485330 DO - https://doi.org/10.1002/chem.201900281 SP - 2 EP - 10 PB - Wiley VCH-Verlag CY - Weinheim AN - OPUS4-48533 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gharaati, S. A1 - Wang, Cui A1 - Förster, C. A1 - Weigert, Florian A1 - Resch-Genger, Ute A1 - Heinze, K. T1 - Triplet–Triplet Annihilation Upconversion in a MOF with AcceptorFilled Channels JF - Chemistry - A European Journal N2 - In summary, we report a highly modular solid TTA-UC system comprising of a crystalline, thermally stable PCN222(Pd) MOF with CA-coated MOF channels and with a DPA annihilator embedded in a solution-like environment in the MOF channels. This solid material displays blue upconverted delayed emission with a luminescence lifetime of 373 us, a threshold value of 329 mW*cm-2 and a triplet–triplet energy transfer efficiency of 82%. This optical application adds another facet to the versatile chemistry of PCN-222 MOFs. The design concept is also applicable to other TTA-UC pairs and enables tuning of the UCL color, for example, by replacing DPA with other dyes as exemplarily shown for 2,5,8,11-tetra-tert-butyl-perylene, that yields UCL at 450 nm. Current work aims to reduce the oxygen sensitivity and to increase the retention of the trapped annihilators in organic environments, for example, by tuning the chain length of the carboxylic acid and by coating the MOF surface. In addition, the TTA-UC efficiency will be further enhanced by reducing the reabsorption of the UC emission caused by Pd(TCPP) and by optimizing the sensitizer/annihilator interface. KW - Porphyrin KW - Method KW - MOF KW - Fluorescence KW - Dye KW - Sensor KW - Oxygen sensitive KW - Single molecule KW - DPA KW - Lifetime KW - Upconverstion KW - Quantum yield KW - Triplet-triplet annihilation KW - Sensitization KW - Energy transfer KW - NMR PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-500580 DO - https://doi.org/10.1002/chem.201904945 VL - 26 IS - 5 SP - 1003 EP - 1007 PB - Wiley-VCH Verlag CY - Weinheim AN - OPUS4-50058 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -