TY - JOUR A1 - Joester, Maike A1 - Seifert, Stephan A1 - Emmerling, Franziska A1 - Kneipp, Janina T1 - Physiological influence of silica on germinating pollen as shown by Raman spectroscopy N2 - The process of silicification in plants and the biochemical effects of silica in plant tissues are largely unknown. To study the molecular changes occurring in growing cells that are exposed to higher than normal concentration of silicic acid, Raman spectra of germinating pollen grains of three species (Pinus nigra, Picea omorika, and Camellia japonica) were analyzed in a multivariate classification approach that takes into account the variation of biochemical composition due to species, plant tissue structure, and germination condition. The results of principal component analyses of the Raman spectra indicate differences in the utilization of stored lipids, a changed mobilization of storage carbohydrates in the pollen grain bodies, and altered composition and/or structure of cellulose of the developing pollen tube cell walls. These biochemical changes vary in the different species. KW - Silica KW - Raman spectroscopy KW - Principal component analysis PY - 2017 U6 - https://doi.org/10.1002/jbio.201600011 SN - 1864-063X SN - 1864-0648 VL - 10 IS - 4 SP - 542 EP - 552 AN - OPUS4-40090 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Lauer, Franziska A1 - Seifert, Stephan A1 - Kneipp, Janina A1 - Weidner, Steffen T1 - Simplifying the preparation of pollen grains for MALDI-TOF MS classification N2 - Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a well-implemented analytical technique for the investigation of complex biological samples. In MS, the sample preparation strategy is decisive for the success of the measurements. Here, sample preparation processes and target materials for the investigation of different pollen grains are compared. A reduced and optimized sample preparation process prior to MALDI-TOF measurement is presented using conductive carbon tape as target. The application of conductive tape yields in enhanced absolute signal intensities and mass spectral pattern information, which leads to a clear separation in subsequent pattern analysis. The results will be used to improve the taxonomic differentiation and identification, and might be useful for the development of a simple routine method to identify pollen based on mass spectrometry. KW - MALDI KW - Conductive carbon tape KW - Pollen KW - Sample pretreatment KW - Principal component analysis PY - 2017 U6 - https://doi.org/10.3390/ijms18030543 SN - 1422-0067 SN - 1661-6596 VL - 18 IS - 3 SP - 543-1 EP - 543-11 PB - MDPI AN - OPUS4-41733 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -