TY - GEN A1 - Taube, Peter T1 - Development of columns based on glass monoliths for affinity chromatography N2 - The aim of this work was the preparation of a novel stationary monolithic phase for affinity chromatography and HPLC-applications. Therefore, we have chosen porous glass filters that are available with different pore sizes as raw materials to prepare monolithic columns. We purchased VitraPOR 4 (10-16 μm pore size) and VitraPOR 5 (1.0-1.6 μm pore size) monolithic glass filters. The physical properties of these glass filters were characterized. The surface area, pore size distribution and the porosity were determined using mercury intrusion porosimetry and BET. These glass filters only exhibit flow through pores and therefore show no bimodal pore size distribution in the mercury intrusion curves. Due to their low permeability, the applied filters that exhibit an inner diameter of 8.0 mm and a length of 15.0±0.1 mm could be operated at flow rates more than 10 ml/min. High flow rates are favorable for fast separation experiments. KW - Monolith KW - Affinity chromatography KW - Affinity extraction KW - Borosilicate glass KW - Sintered KW - Surface area KW - Flow rate KW - VitraPOR KW - Pressure KW - Protein A KW - Dynamic binding capacity KW - Protein A cys KW - Column regeneration KW - Performance comparison PY - 2017 SP - 1 EP - 102 PB - Humboldt-Universität zu Berlin CY - Berlin AN - OPUS4-54564 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -