TY - CONF A1 - Kastanias, Elaine A1 - Elert, Anna Maria A1 - Sturm, Heinz A1 - Özcan Sandikcioglu, Özlem T1 - Chemical and electrochemical interaction mechanisms of metal-reducing bacteria with gold surfaces N2 - The ubiquity of biofilms is accompanied by significant health and safety risks. Clarifying the interaction mechanisms of sessile bacteria with metal and metal oxide surfaces is of great interest to advance the development of new control strategies and novel anti-fouling surfaces which mitigate the detrimental effects of bio-fouling. The Shewanella genus is a faculatively aerobic, Gram-negative, exoelectrogenic bacterium known to demonstrate exceptional adaptability in respiratory capacities. Shewanella is often found in microbial communities within aquatic and sedimentary settings. During aerobic conditions, Shewanella utilizes oxygen as a terminal electron acceptor. During anaerobic conditions, respiration occurs via reduction of alternative terminal electron acceptors such as oxidized metals via extracellular electron transfer mechanisms involving electron shuttle molecules released by the bacteria. The purpose of this work is to investigate the mechanisms of bacterial attachment and subsequent biofilm formation on metallic surfaces. Using Shewanella sp. model biofilms, surface enhanced Raman spectroscopy (SERS) complemented with electrochemical techniques have been utilized to study this dynamic interaction. Additionally, to study the process of diffusion of extracellular electron shuttles, a model system comprised of calcium cross-linked alginate to mimic the architecture of bacterial extracellular polymeric substances has been used. Inert model substrates with consistent uniformity and high surface enhancement factor have been fabricated via the deposition of gold nano-islands on thin glass slides. Moreover, it has permitted modification of the surface chemistry and surface energy via surface functionalization using organothiol self-assembled monolayers. The presentation will summarize our results on the interplay between the initial settlement rate and biofilm formation kinetics of bacteria on metallic substrates with variable environmental conditions such as electrolyte composition, pH, in addition to surface characteristics such as hydrophobicity/hydrophilicity and external polarization. T2 - EUROCORR 2017 & 20thICC CY - Praha, Czech Republic DA - 04.09.2017 KW - Surface Enhanced Raman Spectroscopy (SERS) KW - Metal Reducing Bacteria (MRB) KW - Electron transfer PY - 2017 AN - OPUS4-43421 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -