TY - CONF A1 - Sameith, Janin T1 - Microbially induced corrosion (MIC) - Bacterial biofilms - the good, the ugly and the bad… N2 - Introduction on BAM developments in the project “Microbially Induced Corrosion” (MIC) for new analytical tool, surface modifications and bacterial biofilm developments in the course of the ICBM-Colloquia at the Institut für Chemie und Biologie des Meeres (ICBM) of the Carl-von-Ossietzky-University in Oldenburg. T2 - ICBM Kolloquium (Carl-von-Ossietzky-Universität) CY - Oldenburg, Germany DA - 25.01.2017 KW - MIC KW - Material PY - 2017 AN - OPUS4-39413 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Sameith, Janin T1 - BAM strategies for studies on microbial corrosion - Focus area: Materials - Microbially induced corrosion (MIC) N2 - Presentation of the MIC-project (Microbially Induced Corrosion) within the focus area Materials of the BAM. Strategies, developments and innovations in the area of analytical tools, surface modifications, polymer stability and biofilm-enhanced deteriorations of materials in the context of microbially induced corrosion processes. T2 - Gesellschaft für Korrosion e.V Arbeitskreismeeting: Mikrobielle Materialzerstörung CY - Frankfurt; DECHEMA, Germany DA - 09.02.2017 KW - MIC KW - Material KW - Biodetorioration PY - 2017 AN - OPUS4-39415 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Sameith, Janin A1 - Schwibbert, Karin A1 - Tschiche, Harald A1 - Epperlein, Nadja A1 - Menzel, Friederike A1 - Hoffmann, Katrin A1 - Krüger, Jörg A1 - Resch-Genger, Ute A1 - Kunte, Hans-Jörg T1 - Microbially induced corrosion development of new analytical tools to study biofilm formation and corrosion processes N2 - Microbially induced corrosion (MIC) is a severe cost and risk factor for the oil and gas industry, water heat- and cooling systems, waste water management, and infrastructural constructions. The industrial preventions management of MIC focuses on eliminating present microorganisms, which leads to the repeated and time-consuming application of biocides. However, biocides are often only minor effective for microorganisms that live protected from these agents in a biofilm matrix formed on material surfaces. In recent years, microbiologist have renewed our vision on the electrochemical processes underlying material corrosion induced by sulfate reducing bacteria, but the knowledge at the level of multispecies biofilm formation and the effects of material’s properties are still rather scarce. Therefore, an interdisciplinary team of material scientists, chemists and microbiologists, established a MIC-research platform at the Federal Institute for Materials Research and Testing (BAM) to study the effects of material’s properties and biofilm formation on MIC. Using femtosecond laser pulse technology steel surfaces with defined nanostructures were manufactured. The test pieces were incubated using a flow-through chambers setup with different organisms relevant for biofilm studies. Our studies showed, that nanoscale structures severely affect the biofilm thickness and, hence, growth for health threatening organisms like E. coli and S. aureus, but also for MIC-relevant bacteria such as Fe(III)-reducing Shewanella spec. Another aspect of the biofilm matrix is the establishment of microscale environments with conditions, such as anaerobic or acidic microenvironments, which promotes a diverse multispecies community. However, the detection and visualization of such small-scale microenvironments is rather challenging. Further, it could directly indicate the activity of microorganism influencing or inducing corrosion processes. Therefore, dye-stained nanoscale particles, bearing pH-responsive fluorophores at the surface, were developed to measure the pH at the biofilm’s surface and within the matrix using confocal laser scanning microcopy. The particles will be further advanced to measure other environmentally relevant parameters, including oxygen concentration, and concentrations of selected metal ions indicative of corrosion of e.g., materials used for water pipelines. In the future, our platform and its analytical tools will be employed to study the formation of biofilms in dependency of the respective support material, its surface properties like roughness, and the microbial community. T2 - VAAM Jahrestagung CY - Würzburg, Germany DA - 05.03.2017 KW - MIC KW - Biophotonik KW - LIPSS KW - Nanoparticel based pH-probes PY - 2017 AN - OPUS4-39417 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Epperlein, Nadja A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Koter, Robert A1 - Bonse, Jörn A1 - Sameith, Janin A1 - Krüger, Jörg A1 - Toepel, Jörg T1 - Influence of femtosecond laser produced nanostructures on biofilm growth on steel JF - Applied Surface Science N2 - Biofilm formation poses high risks in multiple industrial and medical settings. However, the robust nature of biofilms makes them also attractive for industrial applications where cell biocatalysts are increasingly in use. Since tailoring material properties that affect bacterial growth or its inhibition is gaining attention, here we focus on the effects of femtosecond laser produced nanostructures on bacterial adhesion. Large area periodic surface structures were generated on steel surfaces using 30-fs laser pulses at 790 nm wavelength. Two types of steel exhibiting a different corrosion resistance were used, i.e., a plain structural steel (corrodible) and a stainless steel (resistant to corrosion). Homogeneous fields of laser-induced periodic surface structures (LIPSS) were realized utilizing laser fluences close to the ablation threshold while scanning the sample under the focused laser beam in a multi-pulse regime. The nanostructures were characterized with optical and scanning electron microscopy. For each type of steel, more than ten identical samples were laser-processed. Subsequently, the samples were subjected to microbial adhesion tests. Bacteria of different shape and adhesion behavior (Escherichia coli and Staphylococcus aureus) were exposed to laser structures and to polished reference surfaces. Our results indicate that E. coli preferentially avoids adhesion to the LIPSS-covered areas, whereas S. aureus favors these areas for colonization. KW - Laser-induced periodic surface structures KW - Femtosecond laser KW - Steel KW - Biofilms KW - Microbial adhesion tests PY - 2017 UR - http://www.sciencedirect.com/science/article/pii/S0169433217305470 DO - https://doi.org/10.1016/j.apsusc.2017.02.174 SN - 0169-4332 SN - 1873-5584 VL - 418 IS - Part B SP - 420 EP - 424 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-40565 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -