TY - CONF A1 - Schneider, Rudolf T1 - Advanced materials broadening the scope of antibody-based analytical methods N2 - Immunoanalytical Techniques, i.e., antibody-based analytical methods, have been used for decades in clinical diagnostics. What makes them attractive for other fields of application is their short time-to-result and high sensitivity. Microplate-based assays such as ELISA have been adopted early in environmental and food analysis. Yet, to make immunoassays even faster, more sensitive, robust, and, most desirable, portable, advanced materials, sometimes developed for other purposes, can be profitably used to achieve these goals. Materials can be novel labels, e.g., chemical or particle labels, such as fluorophores or nanoparticles. Carrier particles, such as magnetic or polymer beads, make it possible to adopt the assays to meso- or microfluidic set-ups and encoding them opens the path to multiplex analysis. Specialty electrodes can enable for higher sensitivity in electrochemical detection. All this broadens the scope of application and lowers effort and cost for analysis at the point-of-need. T2 - ChemForum - Kolloquium des Instituts für Strukturchemie CY - Lisbon, Portugal DA - 07.09.2022 KW - Immunoassay KW - Biosensor KW - Antibody PY - 2022 AN - OPUS4-56782 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Field Analysis by Antibody-based Analytical Methods: The Importance of Advanced Materials N2 - Analytical methods based on the selectivity of antibodies, often called immunoassays, are a back-bone of clinical laboratory diagnostics. To bring them to the field, i.e., to make immunoanalytical methods portable, hopefully even faster, more sensitive, and robust, advanced materials are re-quired. Materials can be novel labels, e.g., chemical or particle labels, such as fluorophores or na-noparticles. Carrier particles, such as magnetic or polymer beads, make it possible to adopt the as-says to meso- or microfluidic set-ups and encoding them opens the path to multiplex analysis. Spe-cialty electrodes can enable for higher sensitivity in electrochemical detection. Without research into better materials, efforts to bring analysis to the point-of-need will not bear fruit. T2 - Kolloquium des Aveiro Institut of Materials (CICECO) der Universität Aveiro CY - Aveiro, Portugal DA - 20.09.2022 KW - Immunoassay KW - Biosensor KW - Antibody PY - 2022 AN - OPUS4-56785 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Arai, Marylyn Setsuko A1 - Kim, Hyunho A1 - Pascavis, Madeleine A1 - Cha, Baekdong A1 - Brambilla, Gabriel A1 - Cho, Young Kwan A1 - Park, Jinho A1 - Vilela, Raquel R. C. A1 - de Camargo, Andrea S. S. A1 - Castro, Cesar M. A1 - Lee, Hakho T1 - Upconverting Nanoparticle-based Enhanced Luminescence Lateral-Flow Assay for Urinary Biomarker Monitoring N2 - Development of efficient portable sensors for accurately detecting biomarkers is crucial for early disease diagnosis, yet remains a significant challenge. To address this need, we introduce the enhanced luminescence lateral-flow assay, which leverages highly luminescent upconverting nanoparticles (UCNPs) alongside a portable reader and a smartphone app. The sensor’s efficiency and versatility were shown for kidney health monitoring as a proof of concept. We engineered Er3+- and Tm3+-doped UCNPs coated with multiple layers, including an undoped inert matrix shell, a mesoporous silica shell, and an outer layer of gold (UCNP@mSiO2@Au). These coatings synergistically enhance emission by over 40-fold and facilitate biomolecule conjugation, rendering UCNP@mSiO2@Au easy to use and suitable for a broad range of bioapplications. Employing these optimized nanoparticles in lateral-flow assays, we successfully detected two acute kidney injury-related biomarkers-kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)-in urine samples. Using our sensor platform, KIM-1 and NGAL can be accurately detected and quantified within the range of 0.1 to 20 ng/mL, boasting impressively low limits of detection at 0.28 and 0.23 ng/mL, respectively. Validating our approach, we analyzed clinical urine samples, achieving biomarker concentrations that closely correlated with results obtained via ELISA. Importantly, our system enables biomarker quantification in less than 15 min, underscoring the performance of our novel UCNP-based approach and its potential as reliable, rapid, and user-friendly diagnostics. KW - Biosensor KW - Upconverting nanoparticles KW - Lateral flow KW - Portable sensor KW - Kidney injury PY - 2024 DO - https://doi.org/10.1021/acsami.4c06117 SN - 1944-8244 VL - 16 IS - 29 SP - 38243 EP - 38251 PB - American Chemical Society (ACS) AN - OPUS4-60689 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Paul, Martin T1 - Real time multichannel biomarker detection using photonic integrated circuits on ultra compact CMOS chips N2 - This work presents the real-time, multichannel detection of the biomolecule Neutravidine using photonic integrated circuits on ultra-compact monolithic CMOS chips. The development focuses on implementing bioassays directly on CMOS platforms, enabling highly integrated and scalable biosensing. The presentation will cover key aspects including microfluidic integration, data analysis, surface functionalization, and chip packaging. T2 - World Biosensor Congress CY - Lisbon, Portugal DA - 18.05.2025 KW - Chips KW - Photonics KW - Biosensor KW - Realtime PY - 2025 AN - OPUS4-63777 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hernández García, María Amparo T1 - SAF-based optical biosensor with 3D-printed free-form optics for targeted explosives immuno-detection N2 - Guaranteeing safety and security of citizens requires a significant effort and innovative tools from national and international agencies and governments, especially when it comes to the field of explosives detection. The need to detect Improvised Explosive Devices (IEDs) and Home-made Explosives (HMEs) at a point of suspicion, has grown rapidly due to the ease with which the precursors can be obtained and the reagents synthesised. The limited availability of immunoanalytical tools for HME detection presents an opportunity for the development of new devices, which enable a rapid detection and recognise the target analyte with high specificity and sensitivity. In this work, we introduce an optical biosensor for highly specific and sensitive HME detection. The immunoassay system is placed in a hydrogel environment permeable to the analyte and transparent to light interrogating the fluorescently labelled antibodies. The readout of the immunoanalytical system is realized with Supercritical Angle Fluorescence (SAF), an advanced microscopy technique. To accomplish this, we made use of recent, commercial high resolution (< 22 µm) Liquid Crystal Display 3D printers to fabricate a parabolic optical element with high refractive index (RI>1.5) and transmission values (>90%) from photo-resin. Aiming at a new generation of sensors, which not only can meet the requirements of trace detection, but can also be used for substance identification, the combination of immunoanalytical recognition with SAF detection offers a modularity and versatility that is principally well suitable for the measurements of target analytes at trace levels. T2 - 8th International conference in Biosensing Technology CY - Seville, Spain DA - 12.05.2024 KW - 3D printing KW - Biosensor KW - Fluorescence KW - Explosives PY - 2024 AN - OPUS4-60561 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hernandez Garcia, Maria Amparo T1 - Optical biosensor using free form prototyped elements for targeted explosives immunodetection N2 - Ensuring the safety and security of citizens necessitates a considerable investment of resources and the development of innovative tools by national and international agencies and governments, particularly in the context of explosives detection [1]. The necessity for the detection of improvised explosive devices (IEDs) and homemade explosives (HMEs) at the point of suspicion has increased exponentially due to the simplicity with which the precursors can be obtained, and the reagents synthesised. The restricted availability of immunoanalytical instruments for the detection of homemade explosives (HMEs) offers a valuable opportunity for the development of innovative devices that can rapidly identify and recognise the target analyte with high specificity and sensitivity [2]. In this study, we present the development of an optical biosensor for highly specific and sensitive HME detection. The immunoassay system is situated within a matrix that is permeable to the target analyte and transparent to light, which enables the interrogation via fluorescence. The immunoanalytical system's readout is achieved through the utilisation of supercritical angle fluorescence (SAF), an advanced microscopy technique. To this end, we employed recent, commercially available high-resolution (less than 22 μm) liquid crystal display SLA printers to fabricate a free-form parabolic optical element with a high refractive index (RI greater than 1.5) and transmission values exceeding 90% from commercial photo-resins. The objective is to develop a new generation of sensors that can not only meet the requirements of trace detection but also be used for substance identification. The combination of immunoanalytical recognition with SAF detection offers a modular and versatile solution that is particularly well suited to the measurement of target analytes at trace levels. T2 - ANAKON 2025 CY - Leipzig, Germany DA - 10.03.2025 KW - Biosensor KW - SAF KW - Free-form optics KW - 3D printing KW - Security PY - 2025 AN - OPUS4-62802 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Paul, Martin T1 - Multichannel biomarker detection using photonic integrated circuits on ultra compact CMOS chips N2 - This work presents the development of a real-time, multichannel detector for biomolecules based on photonic integrated circuits with super-compact CMOS chips. The presentation focuses on using micro-ring-resonators for bioassays, enabling highly integrated and scalable biosensing-solutions. The talk also covers major challenges like including microfluidic integration, data analysis, custom surface functionalization, and chip packaging. T2 - Photonics Week 2025 CY - Berlin, Germany DA - 08.10.2025 KW - CMOS KW - Photonics KW - Biosensor KW - Immunoassay KW - Realtime PY - 2025 AN - OPUS4-64585 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - How can antibodies help us in the risk assessment of pharmaceuticals in the environment? N2 - The widespread use of pharmaceuticals has led to their persistent presence in various environmental compartments, raising concerns about ecological and human health risks since they pose a significant threat to non-target organisms and human health, necessitating robust methods for environmental risk assessment.   Traditional analytical methods, such as liquid chromatography-mass spectrometry (LC-MS), offer high sensitivity and specificity but are often resource-intensive, require complex sample preparation, limiting their applicability for large-scale or real-time monitoring. In this context, antibody-based analytical techniques present a promising complementary approach for the detection and quantification of pharmaceutical residues in environmental matrices.   Antibodies, due to their high specificity and affinity, enable the development of immunoassays such as ELISA, lateral flow assays, fluorescence-based formats, and electrochemical sensors that are rapid, cost-effective, and adaptable to field conditions. These methods can be tailored to target a wide range of pharmaceutical compounds, including antibiotics and endocrine disruptors. Moreover, antibody-based assays facilitate high-throughput screening and can be integrated into portable platforms for on-site analysis, thereby enhancing temporal and spatial resolution in environmental monitoring.   This presentation explores the potential of antibodies in environmental risk assessment, focusing on their application in detecting trace levels of pharmaceuticals in water, soil, and biota. They are presented as a complementary and powerful tool for rapid, cost-effective screening in the lab and that also can be deployed for on-site analysis in the field.   The talk also discusses recent advances in antibody engineering, multiplexing capabilities [1], and the integration of immunoassays with sensor technologies [2].   Case studies illustrate how immunoanalytical data can help in finding potential contamination hotspots [3], hazard identification, pollution source tracking, and the generation of crucial data for predictive models on environmental fate and effects. Antibody-based methods can also help in early warning systems and the evaluation of mitigation strategies, contributing to a more comprehensive and proactive risk assessment framework, eventually to regulatory decision-making, ultimately aiding in the protection of ecosystems and public health. T2 - ICRAPHE - 6th International Conference on Risk Assessment of Pharmaceuticals in the Environment CY - Aveiro, Portugal DA - 20.10.2025 KW - Antibodies KW - Immunoassay KW - Microfluidics KW - Electrochemical detection KW - Biosensor PY - 2025 AN - OPUS4-64922 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schroeder, Barbara A1 - Le Xuan, Hoa A1 - Völzke, Jule L. A1 - Weller, Michael G. T1 - Preactivation Crosslinking - An Efficient Method for the Oriented Immobilization of Antibodies N2 - Crosslinking of proteins for their irreversible immobilization on surfaces is a proven and popular method. However, many protocols lead to random orientation and the formation of undefined or even inactive by-products. Most concepts to obtain a more targeted conjugation or immobilization requires the recombinant modification of at least one binding partner, which is often impractical or prohibitively expensive. Here a novel method is presented, which is based on the chemical preactivation of Protein A or G with selected conventional crosslinkers. In a second step, the antibody is added, which is subsequently crosslinked in the Fc part. This leads to an oriented and covalent immobilization of the immunoglobulin with a very high yield. Protocols for Protein A and Protein G with murine and human IgG are presented. This method may be useful for the preparation of columns for affinity chromatography, immunoprecipitation, antibodies conjugated to magnetic particles, permanent and oriented immobilization of antibodies in biosensor systems, microarrays, microtitration plates or any other system, where the loss of antibodies needs to be avoided, and maximum binding capacity is desired. This method is directly applicable even to antibodies in crude cell culture supernatants, raw sera or protein-stabilized antibody preparations without any purification nor enrichment of the IgG. This new method delivered much higher signals as a traditional method and, hence, seems to be preferable in many applications. KW - Antibody coating KW - Proximity-enhanced reaction KW - Immunoglobulins KW - IgG KW - Protein A KW - Protein G KW - Immunoprecipitation KW - Immunocapture KW - Regeneration KW - Biosensor KW - Immunosensor KW - Affinity chromatography KW - Immunoaffinity extraction KW - Oriented immobilization KW - Immunoassay KW - Bioconjugation PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-479786 DO - https://doi.org/10.3390/mps2020035 SN - 2409-9279 VL - 2 IS - 2 SP - 1 EP - 14 PB - MDPI CY - Basel, Switzerland AN - OPUS4-47978 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - INPR A1 - Schroeder, Barbara A1 - Le Xuan, Hoa A1 - Völzke, Jule L. A1 - Weller, Michael G. T1 - Preactivation crosslinking – An efficient method for the oriented immobilization of antibodies N2 - Crosslinking of proteins for their irreversible immobilization on surfaces is a proven and popular method. However, many protocols lead to random orientation and the formation of undefined or even inactive by-products. Most concepts to obtain a more targeted conjugation or immobilization requires the recombinant modification of at least one binding partner, which is often impractical or prohibitively expensive. Here a novel method is presented, which is based on the chemical preactivation of Protein A or G with selected conventional crosslinkers. In a second step, the antibody is added, which is subsequently crosslinked in the Fc part. This leads to an oriented and covalent immobilization of the immunoglobulin with a very high yield. Protocols for Protein A and Protein G with murine and human IgG are presented. This method may be useful for the preparation of columns for affinity chromatography, immunoprecipitation, antibodies conjugated to magnetic particles, permanent and oriented immobilization of antibodies in biosensor systems, microarrays, microtitration plates or any other system, where the loss of antibodies needs to be avoided, and maximum binding capacity is desired. This method is directly applicable even to antibodies in crude cell culture supernatants, raw sera or protein-stabilized antibody preparations without any purification nor enrichment of the IgG. This new method delivered much higher signals as a traditional method and, hence, seems to be preferable in many applications. KW - Antibody coating KW - Proximity-enhanced reaction KW - Immunoglobulins KW - IgG KW - Protein G KW - Protein A KW - Immunoprecipitation KW - Immunocapture KW - Stabilization KW - Biosensor KW - Biochip KW - Microarray KW - ELISA KW - Immunoassay KW - Immunosensor KW - Crosslinker KW - Nanoparticles KW - Click chemistry KW - Herceptin KW - Trastuzumab PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-478797 DO - https://doi.org/10.20944/preprints201904.0205.v1 SN - 2310-287X SP - 1 EP - 17 PB - MDPI CY - Basel AN - OPUS4-47879 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -