TY - CONF A1 - Wagner, Sabine A1 - Carrasco, S. A1 - Benito-Peña, E. A1 - Walt, D. R. A1 - Moreno-Bondi, M. C. A1 - Rurack, Knut T1 - Integration of microspheres coated with fluorescent molecularly imprinted polymers with a fiber optic array for the detection of antibiotics N2 - The widespread use of antibiotics in livestock farming leads to trace residues in food products and wastewater, potentially entailing antimicrobial resistance in food-borne pathogens. The determination of antibiotics in aqueous environments and foodstuff is thus of major concern. Analytical assays based on molecularly imprinted polymers (MIPs) have emerged as a valuable tool in this field due to the low production costs, stability, format adaptability and the possibility to imprint and thus their ability to recognize a wide variety of target analytes. With regard to optical sensing technologies, however, MIPs have only been used in considerably few applications, especially in fluorescence sensors. This limitation is basically due to the fact that the incorporation of a fluorescently responding moiety into a polymer matrix is challenging. One way to overcome this limitation is to use tailor-made fluorescent indicator monomers for direct transfer of the binding event into an optical signal. If such a monomer is integrated into a thin MIP-shell on microspheres such core/shell particles can be readily used in advanced multiplexing sensory fiber-optic microarrays. Here, we propose such a fiber-optic microarray based on fluorescent MIP microspheres for antibiotics. The binding behavior and the selectivity of a microarray using these MIP were examined and compared with a non-imprinted polymer (NIP) control, employing the target molecules and other structurally closely related antibiotics. T2 - Europt(r)ode XIII CY - Graz, Austria DA - 20.03.2016 KW - Molecularly imprinted polymers KW - Fiber-optic microarray KW - Fluorescence PY - 2016 AN - OPUS4-37294 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wagner, Sabine A1 - Carrasco, S. A1 - Benito-Peña, E. A1 - Moreno-Bondi, M. C. A1 - Rurack, Knut T1 - Detection of antibiotics by combining fiber-optic array with microparticles coated with fluorescent molecularly imprinted polymers N2 - The widespread use of antibiotics in livestock farming leads to trace residues in food products and wastewater, potentially entailing antimicrobial resistance in food-borne pathogens. The determination of antibiotics in aqueous environments and foodstuff is thus of major concern. We have been developing optical sensors based on molecularly imprinted polymers (MIPs) due to the low production costs, stability, format adaptability and the possibility to imprint and thus their ability to recognize a wide variety of target analytes. As a fluorescently responding moiety in the polymer matrix a tailor-made fluorescent indicator cross-linker for direct transfer of the binding event into an optical signal was used. If such a cross-linker is integrated into a thin MIP-shell on microspheres such core/shell particles can be readily used in advanced multiplexing sensory fiber-optic microarrays. Here, we propose such a fiber-optic microarray based on fluorescent MIP microspheres for antibiotics. The binding behavior and the selectivity of a microarray using these silica core/MIP shell beads were examined and compared with a non-imprinted polymer (NIP) control, employing the target molecules and other structurally closely related antibiotics. T2 - APME2017 CY - Ghent, Belgium DA - 21.05.2017 KW - Molecularly imprinted polymers KW - Multiplexing sensory fiber-optic microarrays KW - Antibiotics PY - 2017 AN - OPUS4-40583 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -