TY - JOUR A1 - Ponader, Marco A1 - Weller, Michael G. T1 - Affinitätschromatografie - Neues Trägermaterial aus Borosilikatglas N2 - Therapeutische Antikörper sind innerhalb weniger Jahre zur wichtigsten pharmazeutischen Produktklasse aufgestiegen. Für 2023 werden weltweite Umsätze von über 200 Milliarden USD erwartet. Auch diagnostische Antikörper sind mittlerweile unverzichtbare Produkte, auf deren Basis zahllose Immunoassays und andere Schnelltests entwickelt wurden. Neben der bereits sehr aufwendigen Herstellung von Antikörpern ist deren Aufreinigung aus komplexen Zellkulturmedien oder Blutseren und -plasmen zu einem Engpass in der Produktion und Nutzung dieser komplexen Proteine geworden. Schnelle und einfache Reinigungsmethoden für Antikörper sind daher sehr gefragt. KW - Affinitätschromatographie KW - Affinitätsextraktion KW - Antikörper KW - Glasmonolith KW - Additive Fertigung KW - IgG KW - Immunglobulin KW - HPLC KW - FPLC KW - biokompatibel KW - Borosilikatglas KW - gesintert KW - Titan-Halterung KW - Druckstabilität PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-477490 UR - https://www.chemiextra.com/ N1 - Geburtsname von Ponader, Marco: Wilke, M. - Birth name of Ponader, Marco: Wilke, M. IS - 4 SP - 16 EP - 17 PB - Sigwerb CY - Zug AN - OPUS4-47749 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Herrmann, Stefan T1 - Charakterisierung zweier monoklonaler Antikörper zur Detektion von Sprengstoffen N2 - In 2019 over 30 000 people were killed or injured by explosions caused by explosives like TNT, PETN, HMX and RDX. Therefore, highly sensitive assays for the detection of TNT are needed. In this study we compared two commercially available TNT antibodies: A1.1.1 and EW75C with a highly optimized indirect competitive ELISA based on a BSA-TNA conjugate. As a result, a precision profile for both antibodies was determined with a LOD of 170 pmol L-1 for the clone A1.1.1 and a LOD of 3,2 nmol L-1 for the clone EW75C. The measurements showed that the clone A1.1.1 is a highly sensitive antibody for the detection of TNT while the clone EW75C does show medium performance at most. In the cross-reactivity characterization of both antibodies many substances, closely related to the structure of TNT were tested. Both antibodies showed strong cross reactivity with trinitroaniline and trinitrobenzene. For the clone A1.1.1, which is known to originate from immunization of mice with an TNP-glycine-KLH conjugate, this has to be expected. Interestingly the clone EW75C, which was not characterized yet, showed similar behavior. This suggests a TNA-conjugate as immunogen for the EW75C antibody as well. None of both antibodies showed cross-reactions to the high explosives PETN, HMX and RDX. Also, the cross-reactions of nitro musks with the antibodies were investigated. Despite their prohibition, nitro musks are still used in Asia especially and are particularly popular in India. The overall superior clone A1.1.1 showed a significant cross-reactivity to musk ambrette. For practical reasons the influence of musk ambrette on this assay when used in natural environment should be investigated. In further experiments, the highly sensitive TNT antibody A1.1.1 was digested with papain to obtain monovalent Fab-fragments. Due to its high stability against the digestion, a custom protocol for the IgG1 subclass of mice, to which the clone A1.1.1 belongs, was developed, resulting in a quantitative digestion of the intact antibody to Fab fragments. The success of the digestion was determined with MALDI-TOF-MS and SDS-PAGE. It was shown that this protocol worked for many different antibodies of IgG1 subclass as well. KW - TNT KW - Trinitrotoluol KW - Nitroaromaten KW - Nitromoschus KW - Duftstoffe KW - ELISA KW - Immunoassay KW - Antibody KW - Explosives KW - Klon A1.1.1 KW - Klon EW75C KW - Fab Fragment KW - Kreuzreaktion KW - Crossreactivity KW - Precision Profile KW - Präzisionsprofil KW - Affinity KW - Affinitätskonstante KW - IC50 PY - 2020 SP - 1 EP - 113 PB - Hochschule für Technik und Wirtschaft Berlin - htw CY - Berlin AN - OPUS4-54556 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Zhou, Xuyang A1 - Kumar, Sourabh A1 - Chen, Xinren A1 - Gault, Baptiste A1 - Dehm, Gerhard A1 - Hickel, Tilmann A1 - Raabe, Dierk T1 - Grain Boundary Transformation Induced by Boron Segregation N2 - Boron enhances the hardenability of low-alloyed steel and reduces embrittlement at low temperatures, at parts-per-million concentration levels. Ist effectiveness arises from segregation to grain boundaries (GBs)-planar defects- between crystals-yet atomic-scale evidence remains limited.We addressed this gap by synthesizing GBs with controllable geometry and orientation, enabling reproducible comparison with and without boron segregation. Differential phase-contrast imaging directly reveals boron at iron GBs, and in-situ TEM heating (20 °C to 800 °C) allows us to track the dynamic evolution of GB structures. We found that boron segregation induces local structural changes and triggers GB phase transformations, as corroborated by calculated GB defect phase diagrams spanning broad ranges of carbon and boron content. Our findings not only bridge a gap in understanding the interplay between GB structure and chemistry but also lay the groundwork for targeted design and passivation strategies in steel, potentially transforming its resistance to hydrogen embrittlement, corrosion, and mechanical failure. KW - Steel KW - Defect Phases KW - Grain Boundary PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-655812 DO - https://doi.org/10.1038/s41467-025-62264-1 VL - 16 SP - 1 EP - 14 PB - Nature Communications AN - OPUS4-65581 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Juds, Carmen T1 - Biocombinatorially selected peptide-polymer conjugates as polypropylene binders N2 - Peptide-polymer bioconjugates combine oligopeptides with synthetic polymer blocks and can be used for various applications in material sciences. In recent years, bioconjugates were applied as compatibilizers and coatings. Biocombinatorial approaches, such as phage display, have been shown to yield strong binding peptides, which exhibit excellent coating properties as peptide-PEO conjugates. Phage display represents a widely exploited strategy to select peptides or proteins that exhibit highly specific affinity to various substrates. Following a phage display experiment, DNA sequencing of binding phage clones is required in order to get the sequence information of the binding peptides. Traditionally, random clone picking followed by Sanger sequencing was applied. However, this method may not necessarily identify the strongest binding clones. Next-generation sequencing made sequencing of whole phage libraries possible, which highly improved the selection of strong binders. Here, we show that the biocombinatorial method of phage display combined with next generation DNA sequencing of whole phage libraries represents a powerful tool for an application in material chemistry. Phage display is used to find specific target binding peptides for polypropylene surfaces (PP). PP binders are of particular interest because thus far gluing or printing on PP is challenging due to its low surface energy. Scripts for sequence data analysis were developed and promising sequences were synthesized as peptide-PEO conjugates. Fluorescence based adsorption experiments on PP surfaces led to the identification of strong binding sequences and a better understanding of the peptide-surface interactions. T2 - 257th National Meeting of the American-Chemical-Society (ACS) CY - Orlando, FL, USA DA - 31.03.2019 KW - Peptides KW - Surfaces KW - Phage Display KW - Peptide Library KW - Screening KW - Glue KW - Paint KW - Polyethylene Glycol KW - PEG KW - Next Generation Sequencing PY - 2019 AN - OPUS4-48837 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Celasun, S. A1 - Remmler, D. A1 - Schwaar, Timm A1 - Weller, Michael G. A1 - Du Prez, F. A1 - Börner, H. G. T1 - Digging into the sequential space of thiolactone precision polymers: A combinatorial strategy to identify functional domains N2 - Functional sequences of precision polymers based on thiolactone/Michael chemistry are identified from a large one-bead one-compound library. Single-bead readout by MALDI-TOF MS/MS identifies sequences that host m-THPC that is a second Generation photo-sensitizer drug. The corresponding Tla/Michael-PEG conjugates make m-THPC available in solution and drug payload as well as drug release kinetics can be fine-tuned by the precision segment. KW - Combinatorial chemistry KW - Combinatorial polymer libraries KW - Sequence-defined oligomer KW - Precision polymer sequencing KW - Pseudo peptides KW - MALDI-TOF KW - ESI MS KW - Mass spectrometry KW - Sequencing KW - PEG KW - Polyethylene glycol KW - Solubilizer KW - Drug KW - Conjugates PY - 2019 DO - https://doi.org/10.1002/anie.201810393 SN - 1521-3773 VL - 58 IS - 7 SP - 1960 EP - 1964 PB - Wiley-VCH CY - Weinheim AN - OPUS4-47323 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hernández García, María Amparo T1 - SAF-based optical biosensor with 3D-printed free-form optics for targeted explosives immuno-detection N2 - Guaranteeing safety and security of citizens requires a significant effort and innovative tools from national and international agencies and governments, especially when it comes to the field of explosives detection. The need to detect Improvised Explosive Devices (IEDs) and Home-made Explosives (HMEs) at a point of suspicion, has grown rapidly due to the ease with which the precursors can be obtained and the reagents synthesised. The limited availability of immunoanalytical tools for HME detection presents an opportunity for the development of new devices, which enable a rapid detection and recognise the target analyte with high specificity and sensitivity. In this work, we introduce an optical biosensor for highly specific and sensitive HME detection. The immunoassay system is placed in a hydrogel environment permeable to the analyte and transparent to light interrogating the fluorescently labelled antibodies. The readout of the immunoanalytical system is realized with Supercritical Angle Fluorescence (SAF), an advanced microscopy technique. To accomplish this, we made use of recent, commercial high resolution (< 22 µm) Liquid Crystal Display 3D printers to fabricate a parabolic optical element with high refractive index (RI>1.5) and transmission values (>90%) from photo-resin. Aiming at a new generation of sensors, which not only can meet the requirements of trace detection, but can also be used for substance identification, the combination of immunoanalytical recognition with SAF detection offers a modularity and versatility that is principally well suitable for the measurements of target analytes at trace levels. T2 - 8th International conference in Biosensing Technology CY - Seville, Spain DA - 12.05.2024 KW - 3D printing KW - Biosensor KW - Fluorescence KW - Explosives PY - 2024 AN - OPUS4-60561 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Juds, Carmen T1 - Finding peptide binders for polypropylene using phage display and next generation sequencing N2 - Phage display is used to find specific target binding peptides for polypropylene (PP) surfaces. PP is one of the most commonly used plastics in the world. Millions of tons are produced every year. PP binders are of particular interest because so far gluing or printing on PP is challenging due to its low surface energy. A phage display protocol for PP was developed followed by Next Generation DNA Sequencing of the whole phage library. Data analysis of millions of sequences yields promising peptide candidates which were synthesized as PEG conjugates. Fluorescence-based adsorption-elution-experiments show high adsorption on PP for several sequences. T2 - BAM PhD Day CY - Berlin-Adlershof, Germany DA - 31.05.2018 KW - Polymer KW - Glue KW - Amplification KW - Illumina KW - PEG KW - Sanger sequencing KW - SALSA KW - Data analysis KW - Fluorescence PY - 2018 AN - OPUS4-45055 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Paul, Martin T1 - Real time multichannel biomarker detection using photonic integrated circuits on ultra compact CMOS chips N2 - This work presents the real-time, multichannel detection of the biomolecule Neutravidine using photonic integrated circuits on ultra-compact monolithic CMOS chips. The development focuses on implementing bioassays directly on CMOS platforms, enabling highly integrated and scalable biosensing. The presentation will cover key aspects including microfluidic integration, data analysis, surface functionalization, and chip packaging. T2 - World Biosensor Congress CY - Lisbon, Portugal DA - 18.05.2025 KW - Chips KW - Photonics KW - Biosensor KW - Realtime PY - 2025 AN - OPUS4-63777 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hernandez Garcia, Maria Amparo T1 - Optical biosensor using free form prototyped elements for targeted explosives immunodetection N2 - Ensuring the safety and security of citizens necessitates a considerable investment of resources and the development of innovative tools by national and international agencies and governments, particularly in the context of explosives detection [1]. The necessity for the detection of improvised explosive devices (IEDs) and homemade explosives (HMEs) at the point of suspicion has increased exponentially due to the simplicity with which the precursors can be obtained, and the reagents synthesised. The restricted availability of immunoanalytical instruments for the detection of homemade explosives (HMEs) offers a valuable opportunity for the development of innovative devices that can rapidly identify and recognise the target analyte with high specificity and sensitivity [2]. In this study, we present the development of an optical biosensor for highly specific and sensitive HME detection. The immunoassay system is situated within a matrix that is permeable to the target analyte and transparent to light, which enables the interrogation via fluorescence. The immunoanalytical system's readout is achieved through the utilisation of supercritical angle fluorescence (SAF), an advanced microscopy technique. To this end, we employed recent, commercially available high-resolution (less than 22 μm) liquid crystal display SLA printers to fabricate a free-form parabolic optical element with a high refractive index (RI greater than 1.5) and transmission values exceeding 90% from commercial photo-resins. The objective is to develop a new generation of sensors that can not only meet the requirements of trace detection but also be used for substance identification. The combination of immunoanalytical recognition with SAF detection offers a modular and versatile solution that is particularly well suited to the measurement of target analytes at trace levels. T2 - ANAKON 2025 CY - Leipzig, Germany DA - 10.03.2025 KW - Biosensor KW - SAF KW - Free-form optics KW - 3D printing KW - Security PY - 2025 AN - OPUS4-62802 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hernandez Garcia, Maria Amparo T1 - Fabrication of low-cost free-form optical elements with standard LCD 3D printer for advanced opto-sensing and imaging applications N2 - Nowadays, more and more complex optical elements are used in optical applications, but this can lead to high costs, a time-consuming manufacturing process and limited availability of unconventional elements. Therefore, in this work, we propose LCD 3D printing as alternative cost-effective technique, which is not only user-friendly but also free from design constrains and enables the fabrication of free-form optics. The tested polymeric materials showed promising results for printed optics and optical applications. In addition, 3D printed optical elements were evaluated in terms of their suitability in selected applications with opto-chemical sensors and imaging techniques, with results comparable to those obtained with the corresponding glass optical elements. T2 - 12th European Optical Society Annual Meeting CY - Naples, Italy DA - 09.09.2024 KW - 3D-printing KW - Optics KW - Photopolymerization KW - Rapid prototyping KW - Sensors PY - 2024 AN - OPUS4-61442 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -