TY - GEN A1 - Canning, S. A1 - Voskuil, J. A1 - Weller, Michael G. T1 - An author and reviewer in conversation – reproducibility and antibody validation T2 - F1000Research Blog N2 - A recent Opinion Article by Jan Voskuil (JV), Aeonian Biotech, discussed the challenges in validation of research antibodies. It was openly peer reviewed by Michael Weller (MW), Federal Institute for Materials Research and Testing (BAM), Germany. In this blog, both discuss these challenges, the reproducibility crisis and open data. DA - 19 May, 2017 KW - Antibodies KW - Monoclonal KW - Antibody ID KW - Quality control KW - Open data KW - Reproducibility KW - Peer review PY - 2017 UR - https://blog.f1000.com/2017/05/19/an-author-and-reviewer-in-conversation-reproducibility-and-antibody-validation SN - 2051-9796 SP - 1 EP - 5 PB - F1000 Research Ltd. CY - London AN - OPUS4-40310 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Sauer, Andreas A1 - Buttler, Sabine A1 - Scharf, Holger A1 - Becker, Roland T1 - Bericht zum 23. BAM-Ringversuch "Altlasten" N2 - Präsentation der Ergebnisse eines Ringversuchs zur Kompetenzbewertung von Prüflaboratorien auf dem Gebiet der anorganischen und organischen Bodenanalytik: Polyzyklische aromatische Kohlenwasserstoffe (PAK) in Boden, Mineralölkohlenwasserstoffe (MKW) in Boden, Elemente in Sediment und Gesamtcyanid in Boden. KW - Gesamtcyanid KW - Boden KW - Eignungsprüfung KW - MKW KW - PAK KW - Schwermetalle PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-473403 SP - 1 EP - 261 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-47340 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Krop, U. A1 - Scherz, Franziska A1 - Weller, Michael G. A1 - Monks, K. T1 - Fast and robust purification of antibodies from human serum with a new monolithic protein A column T2 - Knauer Application Sheet N2 - The use of antibodies is becoming more and more important. Therewith the need for fast and robust methods for their purification and columns with a long lifetime is growing. In this application, we describe a fast and robust protein A affinity chromatography method with a new monolithic column. Antibodies were successfully purified in less than 1.5 minutes with a flowrate of 5 mL/min highlighting the advantages of this column and method. KW - Antibodies KW - Protein A KW - MNPQ KW - Technology transfer KW - Human serum KW - Purification KW - IgG KW - SDS PAGE KW - Regeneration PY - 2017 UR - http://www.knauer.net/en/application/fast_and_robust_purification_of_antibodies_from_human_serum_with_a_new_monolithic_protein_a_column.html SP - 1 EP - 2 PB - Knauer CY - Berlin AN - OPUS4-43163 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert A1 - Müller, L. A1 - Traub, Heike A1 - Esteban-Fernández, D. A1 - Panne, Ulrich A1 - Herrmann, Antje A1 - Schellenberger, E. A1 - Theuring, F. A1 - Kneipp, Janina T1 - Imaging by laser ablation ICP-MS N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. T2 - Ringvorlesung Analytik CY - Humboldt-Universität zu Berlin DA - 23.06.2017 KW - Laser ablation ICP-MS KW - Bio-Imaging PY - 2017 AN - OPUS4-40757 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Traub, Heike A1 - Esteban-Fernandez, Diego A1 - Panne, Ulrich A1 - Herrmann, Antje A1 - Schellenberger, E. A1 - Kneipp, Janina T1 - Lecture 5 Bio- and immuno-imaging by use of laser ablation ICP-MS N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. KW - Imaging KW - Laser ablation ICP-MS PY - 2017 AN - OPUS4-40943 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert T1 - Lecture 7: Speciation N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. KW - Speciation KW - SDS-PAGE KW - GC-ICP-MS PY - 2017 SP - 1 EP - 74 CY - Berlin AN - OPUS4-41721 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Paul, Martin T1 - Microarrays for the screening of monoclonal antibodies from supernatants T2 - Masterarbeit N2 - In this thesis a protocol based on a direct competitive and non-competitive immunoassay using the microarray format was developed to screen emulated hybridoma supernatants against the drug carbamazepine (CBZ). As support, epoxy slides were manufactured by coating microscope glass slides with epoxy silanes. The manufactured epoxy slides showed similar capacity for protein immobilization as commercially available NEXTERION slides, but showed a higher autofluorescence background. On the epoxy slides, protein A, Cys-A, G and Cys-G were immobilized as antibody capturing coating. It could be shown that the Cys-tag considerably increased the immobilization of all compared proteins, especially of protein G. Protein Cys-G immobilized at pH 8-9 was considered the most suitable protein for antibody immobilization due to good spot uniformity and a high binding capacity for IgG. Additionally, in immobilization experiments it could be shown, that 10-500 fold excess of bovine IgG, introduced by two different fetal bovine serum (FBS) products, showed no significant competition on the immobilization of mice or goat IgG on protein Cys-G. To examine the captured antibodies for affinity to CBZ, the CBZ-TOTA-DY654 tracer was custom synthesized. It could be demonstrated, that the tracer antibody interaction was competitive with the tracer precursor, CBZ-TOTA-NH2 and CBZ. Furthermore, the synthesized tracer was also successfully used by externals to evaluate CBZ affine hybriodoma cells in flow cytometry. In the simulated screening, five antibodies with known affinity to CBZ were diluted in a cell culture medium. The screening method allowed to differentiate between affine antibodies (IC50 < 20 µg/ml) and unaffine antibodies (IC50 > 20 µg/ml), when concentrations of 0.1 µg/ml IgG or greater have been used. Of five applied antibodies, all highly affine clones (CE2 and B3212M) were reported as positives while no false-positive samples were observed. The screening included competitive, with around 89500 fold excess of CBZ, and non-competitive tracer incubation on different segments of the same chip, which resulted in total signal suppression on the competitive segment. The screening was performed in 20 hours, only a few nanoliters, of each simulated supernatant, were consumed in the screening process, furthermore in the method standard 96 well MTPs were used and no cleanroom facilities were required. KW - Carbamazepine KW - Microscopic slides KW - Protein A cys KW - Competition KW - Epoxy silanes KW - Supernatant KW - Antibody immobilization PY - 2017 SP - 1 EP - 96 PB - Humboldt-Universität zu Berlin CY - Berlin AN - OPUS4-54645 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Wiggenhauser, Herbert A1 - Kind, Thomas A1 - Mielentz, Frank A1 - Strangfeld, Christoph A1 - Völker, Christoph A1 - Wilsch, Gerd T1 - Non-destructive testing in civil engineering at BAM N2 - Research and Development in Non-Destructive Testing in Civil Engineering at BAM has de-veloped over more than two decades. Highlights of present research are ultrasound phase array probes, ground penetrating radar applications for the assessment of existing structures and Laser Induced Breakdown Spec-troscopy (LIBS) to determine which chemical elements on the surface of a specimen are. RFID sensor devel-opments for wireless moisture monitoring and data fusion for the combined analysis of sensor data are fast developing areas. In addition, a selection of application cases is briefly described. T2 - Vortrag über die Aktivitäten des Fachbereichs 8.2 in der zerstörungsfreien Prüfung im Bauwesen. KW - Ultrasonic testing KW - RFID-sensors KW - Data fusion KW - Ground penetrating radar KW - LIBS PY - 2017 SP - 1 EP - 10 CY - Berlin AN - OPUS4-44132 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert T1 - Non-spectral interferences N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. KW - Matrix effects KW - Internal standards PY - 2017 SP - 1 EP - 7 CY - Berlin AN - OPUS4-40868 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Bienwald, Frank T1 - Optimierung der Aminosäureanalytik aromatischer Aminosäuren mittels HPLC T2 - Masterarbeit N2 - Ausgehend von dem in der Literatur beschriebenen Verfahren zur Bestimmung aromatischer Aminosäuren konnte eine Analysemethode zur Proteinbestimmung über die optische Detektion von Tyrosin und Phenylalanin nach chromatographischer Trennung der Protein-Hydrolysate entwickelt werden, die gegenüber dem Verfahren aus der Literatur erhebliche Vorteile besitzt. Der Einsatz von Rundbodenvials stellt eine Neuerung und kostengünstige Alternative zu der konventionellen Hydrolyse in Vakuumhydrolyseröhrchen wie auch zu den Mikrowellenvials der Mikrowellenhydrolyse dar, die das Potential für Miniaturisierungen der Proben hat und sich zudem für große Probendurchsätze eignet. Anstelle der Mikrowellenheizungen wurde ein konventionelles Ölbad als Heizquelle verwendet. Somit ergibt sich eine Verbesserung auch darin, dass diese Methode geringere Geräteinvestitionen erfordert. Wie die Ergebnisse nahelegen, sind sowohl Salzsäure als auch Bromwasserstoffsäure geeignete Reagenzien, die bei der sauren Hydrolyse von Proteinen verwendet werden können. Mit Hilfe antioxidativer Zusätze können Nebenreaktionen erfolgreich vermieden werden. Hier haben sich L-Cystein und Oxalsäure als wirkungsvolle Additive herausgestellt. Wichtige Verbesserungen betreffen vor allem die Zeitersparnis bei der Hydrolyse, die Aufarbeitung der Hydrolysate und die chromatographischen Separationen. Erstere konnte gegenüber der konventionellen Methode von 22 Stunden auf 45 Minuten reduziert werden. Die chromatographische Trennung wurde so weit optimiert, dass die Laufzeit von 64 auf 35 Minuten verkürzt werden konnte. Zudem konnte auf das zeitraubende und kontaminationsanfällige Eindampfen der Hydrolysate verzichtet werden. Während der Aufarbeitung der Hydrolysate konnten die Menge eingesetzter Chemikalien reduziert und damit mögliche Störungen während der optischen Detektion verringert werden. Letztere wurde zum einen durch das Einbeziehen der UV-Absorption bei 260 nm erweitert, sodass eine Alternative zur Absorption bei 215 nm vorliegt, die ebenere Basislinien im Chromatogramm ermöglicht. Zum anderen wurde gezeigt, dass mit Hilfe der Fluoreszenz die Empfindlichkeit der Methode gegenüber der UV-Absorption erheblich verbessert werden kann. Anhand der Probe eines Birkenpollenextraktes ließ sich die Anwendbarkeit der optimierten Methode auf Realproben demonstrieren. KW - Aromatische Aminosäuren KW - Aromatic amino acid analysis KW - AAAA KW - Phenylalanine KW - Tyrosine KW - Hydrochloric acid KW - Hydrobromic acid KW - HCl KW - HBr KW - Chromatographie KW - Reversed-phase KW - Hydrolysis KW - AdvanceBio Peptide Map KW - PRP-C18 PY - 2017 SP - 1 EP - 71 PB - Humboldt-Universität zu Berlin CY - Berlin AN - OPUS4-54650 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Simon, Franz-Georg T1 - PEREBAR - Long-Term Performance of Permeable Reactive Barriers Used for the Remediation of Contaminated Groundwater N2 - Contaminated land, landfills and sediments pose a serious environmental threat by polluting groundwater in the surrounding area. In 14 European countries contamination caused by uranium represents a particularly serious danger where drinking water resources might be affected. Other heavy metals and organic pollutants can also have a strongly deleterious effect on groundwater. Available technologies (e.g. pump- and-treat) fall short of solving the problem because their performance is not yet adequate for effective remediation. The aim of the project is to elaborate the scientific basis for laboratory and pilot-scale testing of and the practical application of a considerably more efficient and cost-effective in-situ reactive barrier technology targeting the above contaminants. The primary model test site will be an area in Southern Hungary contaminated by uranium mining- thus including a region which is due to become part of the European Union. The approach taken to meet the project objectives was the characterisation of different reactive materials and relevant attenuation processes in the reactive matrix of the permeable barrier with special respect to their long-term behaviour. The experimental work included laboratory experiments at different scales, going from bench-scale tests up to pilot-scale, and field-scale experiments. Experimental conditions were predetermined by the characteristics of the model site, such as geologic and hydrogeological settings, soil composition, and type, extent and spreading of the contamination. Technological methods to enhance the long-term efficacy and cost-effectiveness of permeable reactive barrier systems were developed and tested under realistic conditions. KW - Ground water pollution KW - Contaminated sites KW - Environmental pollution KW - Permeable reactive barrier systems PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-390231 UR - http://www.eugris.info/displayproject.asp?Projectid=4290 SP - 1 EP - 9 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-39023 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Adamczyk, Burkart A1 - Simon, Franz-Georg A1 - Adam, Christian T1 - RecArc - Verwertung von Rückständen aus der Metallurgie mit der Lichtbogentechnologie N2 - In dem Projekt soll die vollständige Wiederverwertbarkeit der Schlacke und ihrer Bestandteile demonstriert werden. Dazu gehören die vollständige Rückgewinnung der metallischen Phase, die in die Edelstahlherstellung zurückgeführt werden kann, sowie die Aufwertung der mineralischen Fraktion, die ihre uneingeschränkte Verwendung als Zementzumahlstoff oder mineralischer Baustoff erlaubt. Bei geeigneter Prozessführung des elektrischen Lichtbogenofens können durch „reduzierendes Schmelzen“ die chromhaltigen Wertstoffe zusammen mit anderen Schwermetallen zu 97% in einer Metalllegierung angereichert und separiert werden. Diese kann als Rohstoff in der Metallurgie eingesetzt werden. Der Chromgehalt der mineralischen Komponte der Schlacke konnte von ca. 3% auf unter 0,1% verringert werden. Die mineralische Fraktion lässt sich durch entsprechende Zuschläge während des Schmelzbetriebes und eine anschließende Granulation der schmelzflüssigen Phase zu einem Material aufwerten, das in seiner Zusammensetzung und seinen Eigenschaften typischen Zementkomponenten entspricht und als Rohstoff in der Zementproduktion eingesetzt werden kann. Dafür müssen allerdings noch die rechtlichen Voraussetzungen geschaffen werden, da der Rohstoffeinsatz gerade im Zement strengen Auflagen unterliegt und die Zemente bestimmte Normen erfüllen müssen. Durch die in RECARC aufgezeigte Möglichkeit der Wiederverwertung können somit Stoffkreisläufe geschlossen werden, die ansonsten zu einem Verlust wertvoller Rohstoffe führen. Vor allem die steigenden Rohstoffkosten, aber auch die immer stärker limitierten CO2-Emissionen zeigen die hohe Relevanz im Umgang mit metallurgischen Reststoffen. Eine Überführung des in RECARC demonstrierten Verfahrens in die Industrie könnte einen wichtigen Beitrag zur nachhaltigen Schonung der natürlichen Ressourcen und der Umwelt leisten. KW - Chrom KW - Lichtbogenofen KW - Recycling KW - Rückgewinnung KW - Schlacke PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-390197 SP - 1 EP - 4 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-39019 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Weller, Michael G. T1 - Referee Report 06 March 2017 T2 - The challenges with the validation of research antibodies N2 - This article is a valuable and welcome contribution to the ongoing discussion of antibody quality. The suggestion of a two-tier approach is helpful to distinguish between simple descriptive data and the validation of an antibody for a specified application. KW - Antibody KW - Validation PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-393333 UR - https://f1000research.com/articles/6-161/v1#referee-response-20349 DO - https://doi.org/10.5256/f1000research.11700.r20349 SN - 2046-1402 SP - 6 EP - 7 PB - F1000 Research Ltd. CY - London AN - OPUS4-39333 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Nowak, S. H. A1 - Petric, M. A1 - Buchriegler, J. A1 - Bjeoumikhov, A. A1 - Bjeoumikhova, Z. A1 - von Borany, J. A1 - Munnik, F. A1 - Radtke, Martin A1 - Renno, A. D. A1 - Reinholz, Uwe A1 - Scharf, O. A1 - Tilgner, J. A1 - Wedell, R. T1 - Road to micron resolution with a color X-ray camera – polycapillary optics characterization T2 - arXiv.org N2 - In a color X-ray camera spatial resolution is achieved by means of a polycapillary optic conducting X-ray photons from small regions on a sample to distinct energy dispersive pixels on a CCD matrix. At present, the resolution limit of color X-ray camera systems can go down to several microns and is mainly restricted by Pixel dimensions. The recent development of an efficient subpixel resolution algorithm allows a release from pixel size, limiting the resolution only to the quality of theoptics. In this work polycapillary properties that influence the spatial resolution are systematized and assessed both theoretically and experimentally. It is demonstrated that with the current technological Level reaching one micron resolution is challenging, but possible. KW - Color X-ray camera KW - Polycapillary optics PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-404404 UR - https://arxiv.org/abs/1705.08939 SN - 2331-8422 SP - 1 EP - 11 PB - Cornell University CY - Ithaca, NY AN - OPUS4-40440 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert T1 - Short course on ICP-MS N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. KW - Fundamentals KW - Theory KW - Basics PY - 2017 SP - 1 EP - 33 CY - Berlin AN - OPUS4-40865 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert A1 - Schmidt, B. A1 - Sötebier, C. A1 - Pergantis, S. A1 - Shigeta, K. T1 - Single particle and single cell ICP-MS N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. KW - Single particle ICP-MS KW - Single cell ICP-MS PY - 2017 AN - OPUS4-40952 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert T1 - Spectral interferences N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. CY - Berlin KW - Spectral interferences KW - High resolution MS KW - Collision and reaction cells PY - 2017 SP - 1 EP - 18 CY - Berlin AN - OPUS4-40866 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Dietrich, P. A1 - Thissen, A. A1 - Kulak, N. A1 - Kjaervik, Marit A1 - Unger, Wolfgang T1 - XPS surface chemical analysis of aqueous solutions with EnviroESCA T2 - SPECS Application Notes N2 - Water and aqueous reagents are essential in any biological process or system. But apart from a few special low vapor-pressure cases, liquids have not been accessible to any technique requiring UHV conditions. EnviroESCA opens up this exciting field of applications. In this paper first results from water based samples are presented as a proof of concept to demonstrate the special capabilities of EnviroESCA analyzing liquid samples. The following solutions were investigated under near ambient pressure conditions: i.) water, ii.) brine, iii.) an oil in water dispersion, iv.) aqueous iron(II) sulfate heptahydrate, and v.) a suspension of nano silver particles in water. KW - Surface Analysis KW - Near Ambient Pressure XPS KW - Aqueous Solutions PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-394603 UR - http://www.enviro.specs.de/cms/upload/bilder/EnviroESCA/Applications/Liquids/Application-Note_EnviroESCA_Aqueous_Solutions.pdf N1 - BAM Mitarbeiter Beitrag im Acknowledgement definiert. IS - #000394 SP - 1 PB - SPECS CY - Berlin AN - OPUS4-39460 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -