TY - CONF A1 - Hoffmann, Katrin A1 - Wegmann, Marc A1 - Hannemann, Mandy A1 - Somma, Valentina A1 - Jochum, Tobias A1 - Niehaus, Jan A1 - Roggenbuck, Dirk A1 - Resch-Genger, Ute T1 - NanoGenotox - Automatable determination of genotoxicity of nanoparticles with DNA-based optical assays N2 - The overall interest in nanotoxicity, triggered by the increasing use of nanomaterials in the material and life sciences, and the synthesis of an ever increasing number of new functional nanoparticles calls for standardized test procedures and for efficient approaches to screen the potential genotoxicity of these materials. Aiming at the development of fast and easy to use, automated microscopic methods for the determination of the genotoxicity of different types of nanoparticles, we assess the potential of the fluorometric γH2AX assay for this purpose. This assay, which can be run on an automated microscopic detection system, relies on the detection of DNA double strand breaks (DSB) as a sign for genotoxicity. Here, we provide first results obtained with broadly used nanomaterials like CdSe/CdS and InP/ZnS quantum dots as well as iron oxide, gold, and polymer particles of different surface chemistry with previously tested colloidal stability and different cell lines like Hep-2 and 8E11 cells, which reveal a dependence of the genotoxicity on the chemical composition as well as the surface chemistry of these nanomaterials. These studies will be also used to establish nanomaterials as positive and negative genotoxicity controls or standards for assay performance validation for users of this fluorometric genotoxicity assay. In the future, after proper validation, this microscopic platform technology will be expanded to other typical toxicity assays. T2 - Nanoworkshop 2018 CY - PTB Berlin-Adlershof, Germany DA - 14.05.2018 KW - Qdots KW - Surface functions KW - Quantification KW - Nanogenotoxicity PY - 2018 AN - OPUS4-45126 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -