TY - JOUR A1 - Alfeld, M. A1 - Eckhardt, H.-S. A1 - Kraft, J. A1 - Maiwald, Michael A1 - Meermann, Björn A1 - Merz, K. A1 - Prikler, S. A1 - Richert, J. A1 - Steiner, G. A1 - von Tümpling, W. T1 - Trendbericht Analytische Chemie N2 - Zusätzlich zu Methodenentwicklung, Miniaturisierung und Kopplungsverfahren zeigen sich die Hyperspektroskopie zusammen mit Imaging‐Verfahren, der Einzelmolekülnachweis und der Einsatz von 3‐D‐Druckern als neue Schwerpunkte. Hinzu kommen künstliche Intelligenz bei Sensoren, Bildgebungsverfahren und Prozesssteuerung sowie die Vernetzung von Analyse‐ und Laborgeräten. Trends und Forschungsthemen aus der analytischen Chemie, zusammengestellt von elf Autoren, koordiniert von Günter Gauglitz. KW - Multielementanalytik KW - Multimodale Analytik KW - Kristallolgraphie KW - Prozessanalytik KW - Prozessindustrie KW - Industrielle Analytik KW - Chemometrik KW - Chemometrie PY - 2020 DO - https://doi.org/10.1002/nadc.20204095786 SN - 1868-0054 VL - 68 IS - 4 SP - 52 EP - 60 PB - Wiley CY - Weinheim AN - OPUS4-50609 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Maiwald, Michael T1 - The internet of things in the lab and in process - The digital transformation challenges for the laboratory 4.0 T1 - Das Internet of Things in Labor und Prozess - Herausforderungen des digitalen Wandels für das Labor 4.0 N2 - It is a fact that much of the time spent on analytical laboratory instrumentation these days goes into system maintenance. Digital transformation could give us more time again for creativity and our actual laboratory work – if we shape it the right way. N2 - Fakt ist: Einen Großteil der Zeit, der an analytischen Laborgeräten verbracht wird, nimmt heute die Systempflege in Anspruch. Der digitale Wandel kann uns endlich wieder mehr Zeit für Kreativität und die eigentliche Laborarbeit geben – wenn wir ihn richtig gestalten. KW - Lab of the Future KW - Digitalisation KW - Automation KW - Data Analysis KW - Instrument Communication KW - Labor der Zukunft KW - Digitale Transformation KW - Automatisierung KW - Gerätekommunikation PY - 2020 IS - 4 SP - 1 EP - 3 PB - Lumitos AG CY - Darmstadt AN - OPUS4-50618 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kondo, M. A1 - Melzer, Michael A1 - Karnaushenko, D. A1 - Uemura, T. A1 - Yoshimoto, S. A1 - Aklyama, M. A1 - Noda, Y. A1 - Araki, T. A1 - Schmidt, O. G. A1 - Sekitani, T. T1 - Imperceptible magnetic sensor matrix system integrated with organic driver and amplifier circuits N2 - Artificial electronic skins (e-skins) comprise an integrated matrix of flexible devices arranged on a soft, reconfigurable surface. These sensors must perceive physical interaction spaces between external objects and robots or humans. Among various types of sensors, flexible magnetic sensors and the matrix configuration are preferable for such position sensing. However, sensor matrices must efficiently map the magnetic field with real-time encoding of the positions and motions of magnetic objects. This paper reports an ultrathin magnetic sensor matrix system comprising a 2 × 4 array of magnetoresistance sensors, a bootstrap organic shift register driving the sensor matrix, and organic signal amplifiers integrated within a single imperceptible platform. The system demonstrates high magnetic sensitivity owing to the use of organic amplifiers. Moreover, the shift register enabled real-time mapping of 2D magnetic field distribution. KW - Imperceptible electronics KW - Magnetic sensor system KW - Electronic skin KW - Organic electronics KW - Giant magnetoresistance PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-503855 DO - https://doi.org/10.1126/sciadv.aay6094 SN - 2375-2548 VL - 6 IS - 4 SP - Paper eaay6094, 1 EP - 10 PB - American Association for the Advancement of Science AN - OPUS4-50385 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Neumann, Patrick P. A1 - Hirschberger, Paul A1 - Bartholmai, Matthias ED - Zemčík, R. ED - Krystek, J. T1 - Influence of rotor downwash on vertically displaced nanobots in flight N2 - One challenge associated with navigating a nano aerial robot swarm indoors in unstructured environments is, i.a., the limited air space. To avoid an over-regulation of the available indoor air space (e.g., prohibit copters to fly above each other), a safety region around each copter must be defined. In this paper, we investigate the impact of the downwash of a nano-drone on the stability of another nearby nano-drone. In the experiments, we found out that this downwash has a negative influence of a second nano-drone in a distance of around 1 m vertically and 0.2 m horizontally. Based on the obtained data, we developed a model describing the above-mentioned safety region to enable a safe operation of the swarm in these environments with fewer constraints. T2 - 36th Danubia-Adria Symposium on Advances in Experimental Mechanics CY - Pilsen, Czech Republic DA - 24.09.2019 KW - Mobile Robot Olfaction KW - Nano aerial robot KW - Swarm KW - Collision-free navigation KW - Safety region model PY - 2020 DO - https://doi.org/10.1016/j.matpr.2020.03.047 VL - 32 IS - 2 SP - 108 EP - 111 PB - Elsevier Ltd. CY - Amsterdam AN - OPUS4-50673 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Maiwald, Michael T1 - On the way to the cyber-physical lab T1 - Auf dem Weg zum cyber-physical Lab N2 - Laboratories tend to be central hubs for chemical, biotechnological, pharmaceutical or foodstuff production. They play a key role in research and development, chemical analysis, quality assurance, maintenance and process control. For process development and optimization, process analytical technology (PAT) has proven to be a powerful tool to improve our understanding of processes, increase productivity, reduce waste and costs and shorten processing times. N2 - Im Allgemeinen sind Laboratorien zentrale Drehscheiben für die chemische, biotechnologische, pharmazeutische oder Lebensmittelproduktion. Sie spielen eine Schlüsselrolle in Forschung und Entwicklung, chemischer Analytik, Qualitätssicherung, Instandhaltung und Prozesskontrolle. Für die Prozessentwicklung und -Optimierung hat sich die Prozessanalysentechnik (PAT) als leistungsfähiges Werkzeug erwiesen, um das Prozessverständnis zu verbessern, die Produktivität zu steigern, Abfall und Kosten zu reduzieren und die Prozesszeit zu verkürzen. KW - Lab of the Future KW - Digitalisation KW - Cyber-physical Lab KW - Smart Laboratoy PY - 2020 SP - 1 EP - 3 PB - Lumitos AG CY - Darmstadt AN - OPUS4-51036 LA - mul AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Silva, C. P. A1 - Carvalho, T. A1 - Schneider, Rudolf A1 - Esteves, V. I. A1 - Lima, D. L. D. T1 - ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment N2 - During the last two decades, studies related to the occurrence and fate of emerging contaminants in the aquatic environment have received great attention from the international scientific community. The monitoring of the presence of these compounds is particularly important since they are known to induce adverse effects in aquatic environments, even at extremely low concentrations. This work aimed to apply a simple and effective methodology, such as enzyme-linked immunosorbent assay (ELISA), in the monitoring of 17a-ethinylestradiol (EE2) and 17b-estradiol (E2) (a synthetic and a natural hormone, respectively), carbamazepine (CBZ, an antiepileptic), cetirizine (CET, an antihistamine) and caffeine (CAF, a stimulant) in water matrices with differing salinity and organic matter contents. ELISA was proven to be a valid and practical tool, especially for screening purposes in contrast to traditional chromatographic techniques which are prohibitively expensive for an application on a broader base. The main originality of this work was to establish seasonal and spatial effects on the occurrence of the referred contaminants by using the effectiveness of ELISA to screen those compounds in samples with different characteristics. This work reports both the seasonal and spatial quantification of the referred contaminants in the aquatic environment of the central region of Portugal, with concentrations ranging as follows: 5–87 ng L-1, for E2, 2–17 ng L-1, for EE2, 10–1290 ng L-1, for CBZ, 10–190 ng L-1, for CET, and 62–6400 ng L-1, for CAF. KW - Estrogen KW - Estradiol KW - Ethinylestradiol KW - Carbamazepine KW - Portugal KW - Wasser PY - 2020 DO - https://doi.org/10.1039/D0AY00426J SN - 1759-9679 VL - 12 IS - 19 SP - 2517 EP - 2526 PB - Royal Society of Chemistry RSC CY - London AN - OPUS4-51340 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Raysyan, Anna A1 - Eremin, S. A. A1 - Beloglazova, N. V. A1 - De Saeger, S. A1 - Gravel, I. V. T1 - Immunochemical approaches for detection of aflatoxin B1 in herbal medicines N2 - Introduction Aflatoxin B1 (AFB1) is a toxic low‐molecular‐weight secondary metabolite of Aspergillus flavus and A. parasiticus. AFB1 was classified as a Group I carcinogen by the World Health Organisation for Research on Cancer in 1993. AFB1 is an unavoidable natural contaminant of some herbal medicine, able to cause serious health issues for humans consuming the related medicine. Objective Therefore, this study aimed to develop an efficient fluorescence polarisation immunoassay (FPIA) and a rapid, low‐cost, and easy‐to‐use membrane‐based flow‐through immunoassay (MBA) for determination of AFB1 in herbal medicine Origanum vulgare L., Rubus idaeus L., Urtica dioica L. and Sorbus aucuparia L. Results A cut‐off level of the developed MBA was 0.8 ppb. Validation of the developed test was performed with blank and spiked samples. Using three naturally contaminated or three artificially spiked samples. The FPIA showed a linear working range of 8.6 to 64 ppb, and a half maximal inhibitory concentration (IC50) of 24 ppb. Conclusion The results were in good correlation with the enzymelinked immunosorbent assay (ELISA) results (the IC50 0.1 ppb). Both the sample preparation and analysis are simple, cost‐effective and easy to perform on‐site in non‐laboratory environments. Liquid chromatography with tandem mass spectrometry (LC‐MS/MS) was used as a confirmatory technique. KW - Fluorescence Polarisation Immunoassay KW - membrane-based flow-through immunoassay KW - Mycotoxins KW - Mykotoxine KW - Schnelltest PY - 2020 DO - https://doi.org/10.1002/pca.2931 SN - 1099-1565 VL - 31 IS - 5 SP - 662 EP - 669 PB - Wiley Online Libary CY - Hoboken, NJ, USA AN - OPUS4-51341 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Raysyan, Anna A1 - Galvidis, I. A. A1 - Schneider, Rudolf A1 - Eremin, S. A. A1 - Burkin, M. A. T1 - Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milk N2 - A lateral flow immunoassay (LFIA) using latex particles labeled with antibody to BSAclarithromycin (CLA) was developed for the rapid simultaneous group determination of six macrolide antibiotics. Optimization of antigen spotting on the membrane and latex probe loading allowed improving visual detectability (vLOD) 100 times, which was 1, 1, 10, 10, 50, and 1000 ng/mL for CLA, roxithromycin, erythromycin, dirithromycin, azithromycin, and oleandomycin in buffer, respectively. The calculated limits of instrumental detection (cLOD) were respectively 0.12, 0.15, 1.4, 2.1, 2.4, and 3.3 ng/mL. To avoid a strong influence of breast milk of a very diverse and variable composition, a sample pretreatment is proposed. The six macrolides mentioned can be visually detected in breast milk after 20 minutes pretreatment at concentrations of 10-1000 ng / ml or instrumentally with cLOD of 4.0, 2.5, 30, 42, 42 and 180 ng/mL. The recovery rate from the spiked samples carried out using a strip scanner device ranged from 71% to 110%, and precision expressed as relative standard deviation was between 3-14%. The described rapid on-site diagnostic assay format can be useful for monitoring the content of antibiotics in breast milk during macrolide treatment to ensure safe breastfeeding of infants. KW - Clarithromycin KW - Roxithromycin KW - Erythromycin KW - Dirithromycin KW - Azithromycin KW - Oleandomycin KW - Macrolide antibiotics KW - Makrolide KW - Antibiotika PY - 2020 DO - https://doi.org/10.1016/j.jpba.2020.113450 SN - 0731-7085 VL - 189 IS - 113450 SP - 1 EP - 9 PB - Elsevier CY - Amsterdam, NL AN - OPUS4-51343 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Jenning, M. A1 - Marklein, B. A1 - Ytterberg, J. A1 - Zubarev, R. A. A1 - Joshua, V. A1 - van Schaardenburg, D. A1 - van de Stadt, L. A1 - Catrina, A. I. A1 - Nonhoff, U. A1 - Häupl, T. A1 - Konthur, Zoltán A1 - Burmester, G. R. A1 - Skriner, K. T1 - Bacterial citrullinated epitopes generated by Porphyromonas gingivalis infection—a missing link for ACPA production N2 - Objectives: Porphyromonas gingivalis (P.g.) is discussed to be involved in triggering self-reactive immune responses. The aim of this study was to investigate the autocitrullinated prokaryotic peptidylarginine deiminase (PPAD) from P.g. CH2007 (RACH2007-PPAD) from a rheumatoid arthritis (RA) patient and a synthetic citrullinated PPAD peptide (CPP) containing the main autocitrullination site as potential targets for antibody reactivity in RA and to analyse the possibility of citrullinating native human proteins by PPAD in the context of RA. Methods: Recombinant RACH2007-PPAD was cloned and expressed in Escherichia coli. Purified RACH2007-PPAD and its enzymatic activity was analysed using two-dimensional electrophoresis, mass spectrometry, immunoblot and ELISA. Autoantibody response to different modified proteins and peptides was recorded and bioinformatically evaluated. Results: RACH2007-PPAD was capable to citrullinate major RA autoantigens, such as fibrinogen, vimentin, hnRNP-A2/B1, histone H1 and multiple peptides, which identify a common RG/RGG consensus motif. 33% of RA patients (n=30) revealed increased reactivity for α-cit-RACH2007-PPAD before RA onset. 77% of RA patients (n=99) presented α-cit-specific signals to CPP amino acids 57-71 which were positively correlated to α-CCP2 antibody levels. Interestingly, 48% of the α-CPP-positives were rheumatoidfactor IgM/anti-citrullinated peptide/protein antibodies (ACPA)-negative. Anti-CPP and α-RACH2007-PPAD antibody levels increase with age. Protein macroarrays that were citrullinated by RACH2007-PPAD and screened with RA patient sera (n=6) and controls (n=4) uncovered 16 RACH2007-PPAD citrullinated RA autoantigens and 9 autoantigens associated with lung diseases. We showed that the α-CPP response could be an important determinant in parenchymal changes in the lung at the time of RA diagnosis (n=106; p=0.018). Conclusions: RACH2007-PPAD induced internal citrullination of major RA autoantigens. Anti-RACH2007-PPAD correlates with ACPA levels and interstitial lung disease autoantigen reactivity, supporting an infection-based concept for induction of ACPAs via enzymatic mimicry. KW - Zitrullin KW - Rheumatoide Arthritis KW - Proteinmodifikation KW - Autoantikörper PY - 2020 DO - https://doi.org/10.1136/annrheumdis-2019-216919 VL - 79 IS - 9 SP - 1194 EP - 1202 PB - BMJ Publishing Group Ltd AN - OPUS4-51372 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gojani, Ardian A1 - Gornushkin, Igor B. T1 - Räumlich aufgelöste Heterodyne-Spektroskopie N2 - Die räumlich aufgelöste Heterodyn-Spektroskopie (SHS) kombiniert dispersive und interferometrische Techniken zur Gewinnung spektroskopischer Informationen und kann in einem anpassbaren Instrument realisiert werden. SHS wird für die chemische Analyse von verschiedenen Materialien mit Hilfe der Laser-induzierten Plasmaspektroskopie (LIBS) und der Raman-Spektroskopie eingesetzt. KW - Raman spectroscopy KW - Spatial heterodyne spectroscopy KW - Laser-induced breakdown spectroscopy PY - 2020 UR - https://analyticalscience.wiley.com/do/10.1002/was.00080118/full VL - 5 SP - 2 EP - 4 PB - Wiley CY - Weinheim AN - OPUS4-51397 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -