TY - CONF A1 - Sieg, H. T1 - Artificial digestion of aluminium-containing nanomaterials and their effects on the gastrointestinal tract in vitro N2 - Although aluminium is one of the most common elements in the biosphere, up to now little is known about its impact on human health. aluminium and its chemical derivatives are highly abundant in food, food contact materials and consumer products. Humans are exposed to aluminium via the gastrointestinal tract (GI tract). Exposition can change substantially due to consumer behavior since aluminium is also a compound of numerous food additives. Recently, aluminium exposition is increasingly considered to cohere with cancer and neurodegenerative disorders. Lately, due to an increasing attentiveness on this topic, limiting values for food additives have been tightened by the EFSA. However, cellular effects of aluminium and especially aluminium-containing nanomaterials, that represent a significant part of chemicals found in food products, are widely unknown and in the focus of our research activities, for example in the bilateral SolNanoTOX project. We established an in vitro simulation system of the GI tract, where nanomaterials undergo the different physiological, chemical and proteinbiochemical conditions of saliva, gastric juice and the intestine. The artificially digested nanomaterials, as well as soluble aluminium chloride as ionic control substance, were subjected to several analytical and biochemical methods to characterize their change of appearance and their cytotoxic effects on intestinal cellular models. We observed the fate of the nanomaterials during typical pH-values of saliva, gastric and intestinal juice with Dynamic light scattering measurements and ICP-MS in the single particle mode. After observable disappearance at pH 2 the particles recovered in the simulated intestinal fluid. The simulation of the GI tract, mainly the change of pH settings, can lead to a certain chemical activation of aluminium that can increase bioavailability in the intestine after oral uptake of aluminium-containing food products. In vitro assays like CTB, MTT and cellular impedance measurements showed that there were no acute cytotoxic effects measurable after a period up to 48h after incubation, comparable to undigested particles. In contrast, high amounts of aluminium ions showed synergistic effects on cell viability compared to non-digested aluminium ions. Although toxicological potential of Al ions to healthy tissue appears to be low, increased hazardous potential cannot be ruled out to pre-damaged tissue and can have a relevance in risk assessment for special consumer groups with for example chronical intestinal inflammation or dietary eating behavior combined with high exposure to Al-containing food products. T2 - 82nd Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology CY - Berlin, Germany DA - 29.02.2016 KW - Nanoparticles KW - Digestion KW - SAXS KW - Cytotoxicity PY - 2016 AN - OPUS4-36026 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Isecke, Bernd T1 - Art der Korrosion T2 - Deutscher Verband für Schweißtechnik DVS Sektion Berlin CY - Brandenburg, Germany DA - 2006-01-28 PY - 2006 AN - OPUS4-12144 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Arroyo-Abad, Uriel T1 - Arsenolipids in marine fish: Occurence and Perspective T2 - Anakon 2015 CY - Graz, Austria DA - 2015-03-23 PY - 2015 AN - OPUS4-33647 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Arroyo-Abad, Uriel T1 - Arsenolipids in marine fish: Occurence and Perspective T2 - CANAS - Colloquium Analytische Atomspektroskopie CY - Leipzig, Germany DA - 2015-03-08 PY - 2015 AN - OPUS4-33648 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Piechotta, Christian T1 - Arsenolipide im Hering - Eine Quelle für kanzerogene Arsenverbindungen im Urin? T2 - ANAKON 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-27858 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Arroyo-Abad, Uriel T1 - Arsenolipid pattern in fresh cod liver: application of RP-HPLC with simultaneous ICP-MS and ESI-Q-TOF-MS detection T2 - Metallomics 2013 CY - Oviedo, Spain DA - 2013-07-08 PY - 2013 AN - OPUS4-28774 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne T1 - Arsenobetainbestimmung in Fisch mittels LC-ESI-MS/MS T2 - CIA CY - Berlin, Germany DA - 2011-09-26 PY - 2011 AN - OPUS4-24419 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne T1 - Arsenobetainanalytik unter Einsatz eines 13C-markierten Kalibrierstandard für Elektrospray-Massenspektrometrie T2 - Doktorandenseminar des AK Separation Science der GDCh CY - Hohenroda, Germany DA - 2012-01-08 PY - 2012 AN - OPUS4-25662 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kappler, A. T1 - Arsenic redox transformation by humanic substances and Fe minerals T2 - Ninth International Symposium on the Geochemistry of the Earth's Sureface (GES-9) CY - Boulder, CO, USA DA - 2011-06-01 PY - 2011 AN - OPUS4-25154 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Piechotta, Christian T1 - Arsenic and Mercury speciation in fish T2 - GSF Speciation 2008 CY - Munich, Germany DA - 2008-05-25 PY - 2008 AN - OPUS4-17790 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -