TY - CONF A1 - Carl, Peter A1 - Schneider, Rudolf T1 - A bead suspension array for measuring emerging pollutants and anthropogenic markers in wastewater. N2 - The assessment of water quality demands for high-throughput and multi-target compatible analytical methods. On the one hand, the influence of sewage on natural surface waters must be routinely monitored - according to the European Water Framework Directive. On the other hand, estimation of removal efficiencies of pollutants, such as drug residues, is in the focus of industrial and public wastewater treatment. Immunoassays, such as ELISA, are often applied in this area owing to their cost-efficiency and high-throughput capability. However, it is only possible to measure a single analyte in one measurement. In contrast, array technology is capable for measuring multiple substances in parallel. Here we present a four-plex bead-based flow cytometric assay for measuring three drugs (carbamazepine, an anti-epileptic drug, diclofenac, an antiphlogistic and caffeine, a psychoactive substance) and the bile acid isolithocholic acid, which is proposed to be used as a fecal marker. In this suspension array core-shell particles, consisting of a polystyrene core and a silica shell are used. They can be easily encoded with a fluorophore which is introduced to the core and functionalized with amino groups on the surface for creating binding sites for antibodies: Therefore, the marker substances are bound to the amino groups via NHS chemistry. For the determination of the pollutants, the beads are incubated with a mixture of the analyte-specific antibodies. The antibodies undergo a competitive reaction to bind the immobilised molecules on the surface of the beads or the free analyte in the solution, respectively. Bound antibodies can then be visualized via fluorophore-labelled secondary antibodies. For read-out a flow cytometer is used to virtually separate the different encoded beads and to measure the resulting signal intensity of the immunoassay. In order to obtain highly selective binding of the antibodies, we investigated different types of surface modifications to overcome unspecific binding, finding that a PEG-based surface is suitable to support our immunoassay format. The resulting multiplexing assay is appropriate to detect the marker substances in the low µg/L range. T2 - The Singlet Oxygen Strategy: From Singlet Oxygen to Sustainable Oxidation CY - Gent, Belgium DA - 15.06.2016 KW - Bead-based assay KW - Small-molecule sensors KW - Immunoassay KW - Anthropogenic markers KW - Wastewater PY - 2016 AN - OPUS4-36753 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Carl, Peter A1 - Sarma, Dominik A1 - Rurack, Knut A1 - Schneider, Rudolf T1 - A bead suspension array for measuring emerging pollutants and anthropogenic markers in wastewater N2 - Background: The assessment of water quality demands for high-throughput and multi-target compatible analytical methods. On the one hand, the influence of sewage on natural surface waters must be routinely monitored - according to the European Water Framework Directive. On the other hand, estimation of removal efficiencies of pollutants, such as drug residues, is in the focus of industrial and public wastewater treatment. Immunoassays, such as ELISA, are often applied in this area owing to their cost-efficiency and high-throughput capability. However, it is only possible to measure a single analyte in one measurement. In contrast, array technology is capable for measuring multiple substances in parallel. Here we present a four-plex bead-based flow cytometric assay for measuring three drugs (carbamazepine, an anti-epileptic drug, diclofenac, an antiphlogistic and caffeine, a psychoactive substance) and the bile acid isolithocholic acid, which is proposed to be used as a fecal marker. The drugs can be detected from 1 to 4 µg/L and the bile acid can be detected up to 72 µg/L in wastewater, requiring a highly sensitive detection method. Methods: We use core-shell particles as the spherical platform, consisting of a polystyrene core and a silica shell. Such beads can easily be encoded with an organic fluorophore in the core and functionalized with amino groups on the silica shell surface for creating binding sites for antibodies: Therefore, the marker substances are bound to the amino groups via NHS chemistry. The hybrid particles were synthesized in house. Polystyrene was chosen as core material because of its low density and high refractive index, both important requirements for the use in suspension arrays. Easy surface modifications using silane chemistry are accessible on the silica shell. For the determination of the pollutants, the beads are incubated with a mixture of the analyte-specific antibodies. The antibodies undergo a competitive reaction to bind the immobilized molecules on the surface of the beads or the free analyte in the solution, respectively. Bound antibodies can then be visualized via fluorophore-labelled secondary antibodies. For read-out a flow cytometer is used to virtually separate the different dye encoded beads and to measure the resulting Signal intensity of the immunoassay. A four-parametric function was used as calibration curve. Results: In order to obtain highly selective binding of the antibodies, we investigated different types of silica shell surface modifications to overcome unspecific binding, finding that a PEG-modified surface is suitable to support our immunoassay format. Multiplexed small molecule detection can therefore be realized in a simple mix and read procedure without washing steps. Conclusion: We presented a multiplexing suspension array for the detection of four marker substances in wastewater samples, based on an indirect immunoassay format with polystyrene core-silica shell microbeads. The resulting multiplexing assay is appropriate to detect the marker substances in the low µg/L range. Therefore, wastewater samples can be measured without further preconcentration. T2 - Innovations Forum Senftenberg 2016 - Enabling Technologies for Multiparameter Analytics CY - Senftenberg, Germany DA - 01.06.2017 KW - Bead-based assay KW - Small-molecule sensors KW - Immunoassay KW - Anthropogenic markers KW - Wastewater PY - 2016 AN - OPUS4-36752 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Andrés Arcones, Daniel A1 - Weiser, M. A1 - s Koutsourelakis, F. A1 - Unger, Jörg F. T1 - A Bayesian Framework for Simulation-based Digital Twins of Bridges N2 - Simulation-based digital twins have emerged as a powerful tool for evaluating the mechanical response of bridges. As virtual representations of physical systems, digital twins can provide a wealth of information that complements traditional inspection and monitoring data. By incorporating virtual sensors and predictive maintenance strategies, they have the potential to improve our understanding of the behavior and performance of bridges over time. However, as bridges age and undergo regular loading and extreme events, their structural characteristics change, often differing from the predictions of their initial design. Digital twins must be continuously adapted to reflect these changes. In this article, we present a Bayesian framework for updating simulation-based digital twins in the context of bridges. Our approach integrates information from measurements to account for inaccuracies in the simulation model and quantify uncertainties. Through its implementation and assessment, this work demonstrates the potential for digital twins to provide a reliable and up-to-date representation of bridge behavior, helping to inform decision-making for maintenance and management. T2 - EUROSTRUCT 2023 CY - Vienna, Austria DA - 25.09.2023 KW - Digital Twin KW - Simulation Models KW - Uncertainty Quantification KW - Model Bias PY - 2023 UR - https://eurostruct.org/eurostruct-2023/ AN - OPUS4-58663 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Reuter, Dirk A1 - Reinholz, Uwe A1 - Riesemeier, Heinrich T1 - A basic physical Model of X-Ray Scattering for improved X-Ray Fluorescence Analysis - Monochromatic Synchrotron Radiation T2 - ICXOM20 - 20th International Congress on X-ray Optics and Microanalysis CY - Karlsruhe, Germany DA - 2009-09-14 PY - 2009 AN - OPUS4-19644 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hodoroaba, Vasile-Dan A1 - Reuter, Dirk T1 - A basic physical Model of X-Ray Scattering for improved X-Ray Fluoescence Analysis - Polychromatic X-Ray Tube Excitation T2 - ICXOM20, 20th International Congress on X-ray Optics and Microanalysis CY - Karlsruhe, Germany DA - 2009-09-14 PY - 2009 AN - OPUS4-19534 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Reuter, Dirk A1 - Hodoroaba, Vasile-Dan A1 - Radtke, Martin A1 - Reinholz, Uwe A1 - Riesemeier, Heinrich T1 - A basic physical Model of X-Ray Scattering for improved X-Ray Fluoescence Analysis T2 - FKA - 15. Tagung Festkörperanalytik CY - Chemnitz, Germany DA - 2009-07-12 PY - 2009 AN - OPUS4-19643 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Binder, Christian A1 - Kieper, Gerhard A1 - Herrmann, Peter T1 - A 500 bar Gaseous Oxygen Impact Test Apparatus for Burn-Out Testing T2 - 7. International Symposium on Flammability and Sensitivity of Materials in Oxygen-Enriched Atmospheres CY - Denver, CO, USA DA - 1995-03-16 PY - 1995 AN - OPUS4-7658 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Weidner, Steffen T1 - ^Mass spectrometry of synthetic polymers applications and limitations of the MALDI-TOF technique T2 - APST 2 (Advances in Polymer Science and Technology) CY - Linz, Austria DA - 2011-09-28 PY - 2011 AN - OPUS4-25271 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - [RES]Check: Dezentrales Diagnostiksystem zur schnellen Erfassung viraler oder bakterieller Erreger respiratorischer Erkrankungen T2 - MEDICA CY - Düsseldorf, Germany DA - 2011-11-16 PY - 2011 AN - OPUS4-25119 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Erhard, Anton A1 - Wüstenberg, Hermann A1 - Mundry, Eberhard T1 - 90°-Longitudinalwellen-Winkelprüfkopf zum Nachweis oberflächennaher Risse T2 - DGZfP Europäischen Vortragstagung Zerstörungsfreie Materialprüfung CY - Mainz, Germany DA - 1978-05-08 PY - 1978 AN - OPUS4-18793 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -