TY - CONF A1 - Ascher, Lena A1 - Häckel, A. A1 - Schellenberger, E. A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - Imaging of Eu doped very small iron oxide nanoparticle in atherosclerotic plaques via LA-ICP-MS N2 - Atherosclerotic lesions can be visualized by magnetic resonance imaging (MRI) using very small iron oxide nanoparticles (VSOP). VSOP accumulate in atherosclerotic plaques and thus serve as an atherosclerosis probe. The aim of this project was to image the distribution of europium doped VSOP (Eu-VSOP) by laser ablation ICP-MS in histological thin cuts of the aortic root region of ApoE knockout mouse model that is rich in atherosclerotic plaques. In addition, it was investigated whether VSOP accumulation in the plaques correlates with other biomarkers of inflammation for example macrophages and altered endothelium to assess whether it correlates with instability or vulnerability of the plaque regions. For this purpose, antibodies were labeled with various lanthanide elements and correlated with the Eu-VSOP distribution using LA-ICP-MS in a multiplex measurement mode. T2 - ESAS/CANAS 2018 CY - Berlin, Germany DA - 20.03.2018 KW - Bioimaging KW - LA-ICP-MS KW - Immunoassay KW - Nanoparticle KW - Labeling PY - 2018 AN - OPUS4-45131 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ascher, Lena A1 - Häckel, Akvile A1 - Schellenberger, Eyk A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - Imaging of Eu doped very small iron oxide nanoparticle in atherosclerotic plaques via LA ICP-MS N2 - Atherosclerotic lesions can be visualized by magnetic resonance imaging (MRI) using very small iron oxide nanoparticles (VSOP). VSOP accumulate in atherosclerotic plaques and thus serve as an atherosclerosis probe. The aim of this project was to image the distribution of europium doped VSOP (Eu-VSOP) by laser ablation ICP-MS in histological thin cuts of the aortic root region of ApoE knockout mouse model that is rich in atherosclerotic plaques. In addition, it was investigated whether VSOP accumulation in the plaques correlates with other biomarkers of inflammation for example macrophages and altered endothelium to assess whether it correlates with instability or vulnerability of the plaque regions. For this purpose, antibodies were labeled with various lanthanide elements and correlated with the Eu-VSOP distribution using LA-ICP-MS in a multiplex measurement mode. A possible correlation of reactive oxygen species (ROS) with endogenous iron or Eu-VSOP can also be detected by LA-ICP-MS. For this purpose, ROS-specific antibodies were also labeled with lanthanides. T2 - BI(MS)2 2018 – First Workshop on Laser Bioimaging Mass Spectrometry CY - Münster, Germany DA - 23.05.2018 KW - Bioimaging KW - LA-ICP-MS KW - Immunoassay KW - Nanoparticle KW - Labeling PY - 2018 AN - OPUS4-45133 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ascher, Lena A1 - Häckel, A. A1 - Schellenberger, E. A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - Imaging of Eu doped very small iron oxide nanoparticle in atherosclerotic plaques via LA-ICP-MS N2 - Atherosclerotic lesions can be visualized by magnetic resonance imaging (MRI) using very small iron oxide nanoparticles (VSOP). VSOP accumulate in atherosclerotic plaques and thus serve as an atherosclerosis probe. The aim of this project was to image the distribution of europium doped VSOP (Eu-VSOP) by laser ablation ICP-MS in histological thin cuts of the aortic root region of ApoE knockout mouse model that is rich in atherosclerotic plaques. In addition, it was investigated whether VSOP accumulation in the plaques correlates with other biomarkers of inflammation for example macrophages and altered endothelium to assess whether it correlates with instability or vulnerability of the plaque regions. For this purpose, antibodies were labeled with various lanthanide elements and correlated with the Eu-VSOP distribution using LA-ICP-MS in a multiplex measurement mode. A possible correlation of reactive nitrogen species (RNS) with endogenous iron or Eu-VSOP can also be detected by LA-ICP-MS. For this purpose, RNS-specific antibodies were also labeled with lanthanides. T2 - 26. ICPMS-Anwendertreffen CY - Berlin, Germany DA - 03.09.2018 KW - Bioimaging KW - LA-ICP-MS KW - Immunoassay KW - Nanoparticle KW - Labeling PY - 2018 AN - OPUS4-45920 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tvrdonova, M. A1 - Vlcnovska, M. A1 - Pompeiano Vanickova, L. A1 - Kanicky, V. A1 - Adam, V. A1 - Ascher, Lena A1 - Jakubowski, Norbert A1 - Vaculovicova, M. A1 - Vaculovic, T. T1 - Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry JF - Analytical and Bioanalytical Chemistry N2 - In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling. KW - Nanoparticle KW - LA-ICP-MS KW - Labeling PY - 2019 DO - https://doi.org/10.1007/s00216-018-1300-7 SN - 1618-2642 SN - 1618-2650 VL - 411 IS - 3 SP - 559 EP - 564 PB - Springer-Verlag GmbH CY - Berlin, Heidelberg AN - OPUS4-47093 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -