TY - CONF A1 - Altmann, Korinna T1 - Plastik in Böden: Mikroplastik-Analytik N2 - Im Vortrag wir eine Methode zur Analyse von Mikroplastik in Böden dargestellt. Es geht dabei um die Probenvorbereitung und Detektion. T2 - Fachgespräch "Plastik in Böden" CY - Online meeting DA - 28.03.2022 KW - Mikroplastik KW - TED-GC/MS KW - Mikroplastik-Analytik KW - Methodenentwicklung Böden PY - 2022 AN - OPUS4-54646 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Plastik in Böden: Mikroplastik-Ergebnisse N2 - In diesem Vortrag geht um die Vorstellung der Ergebnisse des UBA Projektes Plastik in Böden. Es werden Mikroplastik-Gehalte in verschiedenen Böden präsentiert. T2 - Fachgespräch "Plastik in Böden" CY - Online meeting DA - 28.03.2022 KW - Mikroplastik KW - Mikroplastik Böden KW - Mikroplastik-Analytik PY - 2022 AN - OPUS4-54648 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Preliminary results of an interlaboratory comparison on microplastics organised by plasticsfate N2 - Microplastics are everywhere in the environment, but analytics is challenging. Since harmonisation is missing as well es suitable reference materials, BAM did under th umbrella of VAMAS funded by the EU Horizon 2020 project PlasticsFate a ILC for microplastic detection methods. Methods adressed were IR, Raman, Py-GC/MS and TED-GC/MS. The talk gives a first presentation and evaluation on the results. T2 - CUSP annual meeting and conference CY - Utrecht, Netherlands DA - 12.09.2023 KW - Microplastics KW - TED-GC/MS KW - Polymer 3R KW - Reference material KW - ILC on detection methods PY - 2023 AN - OPUS4-60036 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Grimmer, Christoph T1 - Preparation of novel emission reference materials: μ-capsules & impregnated porous materials N2 - Volatile organic compounds (VOCs) emitted by furniture and building materials can cause health issues. For an improvement of indoor air quality low emitting materials should be used. Quality assurance and –control (QA/QC) measures require an emission reference material (ERM) with a predictable emission rate of VOCs. The idea is to use porous materials as ERM, which store the VOCs inside their pores and emit them constantly. T2 - WORKSHOP: METROLOGY FOR INDOOR AIR QUALITY CY - Mol, Belgium DA - 18.10.2023 KW - Emission reference materials KW - Indoor air quality KW - Materials emissions test KW - VOC PY - 2023 AN - OPUS4-59961 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Production of reference materials using a quality by design approach N2 - Accurate analysis of microplastics is based on validated methods and the use of standardized protocols. Therefore, reference materials are essential to determine recovery rates and optimise the existing workflows. The reference materials should mimic the reality in terms of particle properties and concentration and are intended for a special use. The Quality-by-Design approach helps to select the users need and defines a target product profile with mandatory and desired particle properties. We will address different reference material top-down production processes with their limits and challenges for production of materials varying in size ranges of micro- and nanoplastics. T2 - OECD Workshop on Nanoplastics CY - Paris, France DA - 12.11.2025 KW - Microplastics KW - Reference materials KW - Nanoplastics KW - Quality-by-Design PY - 2025 AN - OPUS4-64717 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Production of small micro- and nanoplastic reference materials supporting harmonization and standardization N2 - The MICROPLASTICdays at Ljubliana, Slowenia, were organized in cooperation with the PRIORITY cost action. The topic has been reference materials this time. The talk gives an idea of the preparation of reference materials in micro- and nanoplastic topic to support standardisation and regulation. It defines the term of micro-and nanoplastics and presents different case studies and critical attributes to the production depending on the enduser needs. T2 - MICROPLASTICdays CY - Ljubljana, Slovenia DA - 25.03.2025 KW - Microplastics KW - Nanoplastics KW - Reference materials PY - 2025 AN - OPUS4-62801 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Nordholt, Niclas T1 - Progress on STOP-project WP5 and WP6 at BAM N2 - An overview of the progress that has been made on testing of antimicrobial efficacy and method development in the STOP project at BAM. T2 - STOP project meeting 09/2024 CY - Bukarest, Romania DA - 18.09.2024 KW - Antimicrobial surfaces KW - ISO22196 PY - 2024 AN - OPUS4-61178 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Vogel, Christian T1 - Quantification of Perfluorocarboxylic Acids in Water Samples via Static Headspace Gas Chromatography Coupled to Mass Spectrometry N2 - Perfluorocarboxylic acids (PFCAs) are one of the most prominent and studied subgroups of per- and polyfluoroalkyl substances (PFASs), which have attracted great interest in environmental and toxicology research due to their intensive use in combination with their persistence, mobility and potential threat to ecosystems and human health. The standard method to quantify PFCAs in water samples is liquid chromatography or, for additional quantification of ultrashort-chain PFCAs (carbon chain length of two or three carbon atoms) too, hydrophilic interaction liquid chromatography, in each case coupled with mass spectrometry. However, there are a lot of laboratories using MS coupled to headspace gas chromatography systems (HS-GC-MS), usually to quantify a wide range of volatile organic compounds. While PFCAs themselves are difficult to measure with HS-GC-MS in water samples, this challenge can be solved by derivatizing the acids to more volatile compounds, like esters. This approach could enable laboratories using HS-GC-MS to analyse and monitor PFCAs in environmental water samples. By following this idea, we successfully developed a HS-GC-MS method to quantify PFCAs in ultrapure water. Trifluoric acid (TFA), perfluoropropionic acid (PFPrA), perfluorobutanoic acid (PFBA) and perfluorooctanoic acid (PFOS) served as exemplary analytes of PFCAs, which were derivatized with methanol and concentrated sulfuric acid to form methyl esters. The method was optimized by varying different preparation and measurement parameters, leading to limits of detection and quantification in the two-digit ppt-range for all four analytes. The method was than tested on various real water samples, including tap and bottled water, groundwater, landfill leachate, treated wastewater and impinger water from a sewage sludge incineration experiment. Furthermore, snow samples from a ski slope were analysed too. TFA was the most frequently detected PFAA, with concentrations in the ppt and low ppb range. The detection and quantification of the remaining three PFCAs vary with the sample types and sampling location.Our results show that HS-GC-MS is suitable, and enables laboratories with this technique, to quantify PFCAs water samples. T2 - SETAC Europe CY - Vienna, Austria DA - 11.05.2025 KW - Per- and polyfluoroalkyl substances (PFAS) KW - Groundwater KW - GC-MS PY - 2025 AN - OPUS4-63094 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gerrits, Ruben T1 - Quantifying and modelling growth of a subaerial black fungus N2 - Environmental, black fungi are known for their oligotrophic lifestyle and melanin production and are ubiquitous colonisers of natural and anthropogenic subaerial surfaces like marble monuments, washing machine soap dispensers and solar panels. Even though their growth is generally unappreciated, prevention or inhibition thereof is still to be accomplished. We have chosen the genetically amenable, black fungus Knufia petricola as a model species to study these organisms. Our goal is simple: the quantitative description of its growth on various environmental conditions to create a mathematical model using the iDynoMiCS 2.0 platform which could allow the development of a mitigation strategy. The conditions to be tested are the quantity and type of carbon and nitrogen sources, the pH and the presence of neighbouring colonies. First results showed that colonies grown on agar have the highest extension rates at pH 5, 0.01M NO3 or NH4, and 0.1M glucose or sucrose. Interestingly, extension rates but also CO2 production was unaffected by an increase in the concentration of the C-source from 0.01M to 0.1M. On a microscale, we observed that medium containing more C resulted in a higher quantity of branches produced by a single cell and not in a higher single cell growth rate. This rapidly results in a more compact microcolony versus the more extended colonies on C-deficient media. We believe that such deeper knowledge on the growth of black fungi will help mitigate their material colonisation and deterioration. T2 - IMC12 CY - Maastricht, Netherlands DA - 09.08.2024 KW - Black fungi KW - Modelling KW - Carbon and nitrogen limitation PY - 2024 AN - OPUS4-61170 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Richter, Anja T1 - Reducing Escherichia coli adhesion to PET by modulating spatial periods of laser-induced surface nanoripples N2 - Using nanofiber-like cell appendages, secreted proteins and sugars, bacteria can establish initial surface contact followed by irreversible adhesion and the formation of multicellular biofilms. Here, the stabilizing extracellular biofilm matrix together with physiological changes on the single cell level leads to an increased resilience towards harsh environmental conditions, antimicrobials, the host immune response and established cleaning procedures. Persistent microbial adhesion on e.g., medical implants, in water supply networks or food-processing industry is often associated with chronic inflammation, nosocomial and foodborne infections, enhanced biofouling and product contamination. To prevent persistent microbial colonization, antibacterial surface strategies often target the initial steps of biofilm formation and impede adhesion of single cells before a mature biofilm is being formed. While chemical coatings have been widely used, their restricted biocompatibility for eukaryotic cells and attenuated antibacterial-effects due to compound release limit their areas of application and alternative strategies focus on modified surfaces topographies to impede bacterial adhesion. In this work, we used ns-UV laser treatment (wavelength 248 nm and a pulse duration of 20 ns) to generate laser-induced periodic surface structures (LIPSS) with different submicrometric periods ranging from ~210 to ~610 nm on commercial poly(ethylene terephthalate) (PET) foils. Following structurally and chemically analyses, PET samples were subjected to bacterial colonization studies with Escherichia coli TG1, a bacterial test strain with a strong biofilm formation capacity due to the formation of nanofiber-like cell-appendages (pili). Bacterial adhesion tests revealed that E. coli repellence decisively depends on the spatial periods of the LIPSS with the strongest reduction (~91%) in cell adhesion observed for LIPSS periods of 214 nm. Scanning electron microscopy and additional biofilm studies using a pili-deficient E. coli TG1 strain revealed the importance of extracellular appendages in the bacterial repellence observed here, thus, pointing out new antibiotics-free strategies for antibacterial surfaces by impeding nanofiber-mediated bacterial adhesion. T2 - E-MRS Spring Meeting 2022 CY - Online meeting DA - 30.05.2022 KW - Laser-induced periodic surface structures (LIPSS) KW - Bacterial adhesion tests KW - Bacteria repellent surfaces KW - Polymer foils KW - E. coli PY - 2022 AN - OPUS4-54930 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Reference material candidates for microplastic analysis N2 - Every day, there are new headlines in the media about microplastics (1-1000 µm, ISO/TR 21960:2020) and nanoplastics (< 1 µm, ISO/TR 21960:2020) findings all over the planet with high variations in particle number and mass. The challenges in analytics are very complex, e.g. representative sampling, non-destructive sample preparation with concentrated particles and homogeneous distribution and true detection. All together lead to lacks in harmonization and results, which are hardly comparable. On the other hand, monitoring of microplastics is mandatory in the future strictly regulated by the EU commission in the Drinking water and Wastewater Framework Directive. One step to accurate and precise results will be the development of suitable reference materials mimicking particles in the environment. BAM developed test materials, which are produced by mixing a small portion of microplastic particles with a water-soluble matrix. After solid phase dilution and homogenisation small portions are pressed into tablets and bottled in glass vials (Figure 1). These tablets are well characterized with particle size distribution and SEM images. Additionally, they are tested as reference material candidate according to homogeneity and stability for particle number with µ-IR and µ-Raman as well as on particle mass with Py-GC/MS and TED-GC/MS after ISO Guide 35. Results are promising. The material passed the homogeneity control. No changes are observed within 6 months of storage. The same tested reference material is finally used in sample preparation experiments, where environmental suspended particular matter from surface water or baby milk powders are spiked with the tablets. T2 - ESOPS CY - Berlin, Germany DA - 09.09.2024 KW - Microplastics KW - TED-GC/MS KW - Reference materials KW - Polymer 3R KW - Tablets PY - 2024 AN - OPUS4-61017 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Release of synthetic fibres during washing of typical household textiles: Findings from washing machine tests and resulting policy recommendations N2 - This talk summarizes the experimental setup and the results of one washing experiment under realistic conditions according to microplastic fiber release. T2 - Tackling microplastics in the environment CY - Brussels, Belgium DA - 09.03.2023 KW - Microplastics KW - Fibers of textile origin KW - Washing machine KW - Microplastic fibre release PY - 2023 AN - OPUS4-57204 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Müller, Axel T1 - Representative sampling of roadside soil and determination of tire wear contents by TED-GC/MS N2 - The presentation describes microplastic findings from tyre wear near roads. Both the sampling and the analysis are presented. T2 - Mikroplastik Workshop der Hochschule Zittau CY - Online meeting DA - 25.11.2021 KW - Mikroplastik KW - Reifenabrieb KW - TED-GC/MS KW - Probennahme PY - 2021 AN - OPUS4-53831 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Repräsentative Untersuchungsstrategien für ein integratives Systemverständnis von spezifischen Einträgen von Kunststoffen in die Umwelt (RUSEKU) N2 - Der Vortrag wurde im Rahmen der Abschlusskonferenz des Forschungsschwerpunktes "Plastik in der Umwelt" des BMBF gehalten. Er fasst die Ergebnisse des Verbundprojektes RUSEKU zusammen. Es wurde die Herstellung verschiedener Testmaterialien für die Mikroplastik (MP) Analytik beschrieben. Im weiteren geht es um verschiedene Ansätze MP kostengünstig und schnell zu detektieren, z.B. mit Nahinfrarotspektroskopie. Nanoplastik konnte mit der Transmissionselektronenmikroskopie sichtbar gemacht werden. Für die praxisnahe Beprobung von Gewässern wurde die Fraktionierte Filtration entwickelt. Integrale Beprobungen können mit dem Sedimentationskasten erfolgen. Als Beispiele für die Beprobung realer Gewässer wurde das urbane Abwassersystem Kaiserslautern, Getränkewasser und Waschmaschinenwasser gezeigt. T2 - Abschlusskonferenz des Forschungsschwerpunktes "Plastik in der Umwelt" des BMBF CY - Online meeting DA - 20.04.2021 KW - Mikroplastik KW - TED-GC/MS KW - Probennahme KW - RUSEKU PY - 2021 AN - OPUS4-52546 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Resistance evolution to antimicrobial biocides N2 - This talk discusses resistance evolution to antimicrobial biocides. It shows (i) that biocides show heterogeneous killing facilitating tolerance evolution, (ii) that serial transfer at subinhibitory concentrations is not appropriate to model evolutionary adaptation to disinfection, (iii) that biocides affect rates of mutation and horizontal gene transfer Biocides, and (iv) that antibiotics show strong combination effects. T2 - Evolutionary Biology meets the Antibiotic Crisis vol. 2 CY - Plön, Germany DA - 24.09.2024 KW - Antimicrobial resistance KW - Bacteria KW - Standardization KW - Biocides PY - 2024 AN - OPUS4-61545 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Resistance evolution towards biocides and antimicrobial surfaces N2 - Biocides, including disinfectants and antimicrobial surfaces (AMCs), are important to prevent the spread of pathogens and antimicrobial resistant bacteria via surfaces. However, concerns have been raised about the evolution and selection of resistance against disinfectants and AMCs. In turn, resistance against disinfectants and AMCs can be associated to antibiotic resistance due to cross-resistance and co-resistance. We need to understand the mechanisms and risks of disinfectants and AMCs for resistance and cross-resistance evolution to optimize their application and safeguard their long-term efficacy. We used adaptive laboratory evolution (ALE) experiments based on repeated exposure of bacteria to disinfectants. Our results show that repeated disinfection of E. coli with benzalkonium chloride in suspension results in a 2000-fold increase in survival within 5 exposure cycles. Adaption is linked to the initial presence of persister cells highly tolerant to benzalkonium chloride. We used the same approach to develop standardizable ALE experiments to determine resistance evolution to AMCs. The results highlight rapid adaptation of E. coli and P. aeruginosa towards copper surfaces. Moreover, there are multiple situations in the clinic or in the environment in which biocides and antibiotics co-occur and in which combination effects can shape their antimicrobial activity or their selective effects. Our work with P. aeruginosa shows prevalent combination effects of biocides and antibiotics, ranging from synergy to antagonism and resulting in the selection for or against antibiotic resistant strains. The combination effects are dependent on the biofilm mode-of-growth, manifesting in apparent differences in the structural arrangement of antibiotic sensitive and resistant strains in biofilms exposed to combinations. Furthermore, biocides affect rates of mutation and horizontal gene transfer, thereby having a potential facilitating effect on resistance evolution. Taken together, our work shows that the role of biocides as potential drivers of resistance evolution and selection deserves further study and regulative action. T2 - Eurobiofilms 2022 CY - Palma, Spain DA - 31.08.2022 KW - Antimicrobial resistance KW - Bacteria KW - Biofilms KW - Biocides KW - Antimicrobial surfaces PY - 2022 AN - OPUS4-55608 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Resistance evolution towards biocides and antimicrobial surfaces N2 - This talk discusses resistance evolution towards biocides and antimicrobial surfaces. It shows (i) that biocides affect rates of mutation and horizontal gene transfer, (ii) that biocides show heterogeneous killing facilitating tolerance evolution, and (iii) that biocides and antibiotics show strong combination effect on growth and selection. T2 - STOP project internal seminar CY - Online meeting DA - 24.04.2024 KW - Antimicrobial resistance KW - Bacteria KW - Standardization KW - Biocides KW - Antimicrobial surfaces PY - 2024 AN - OPUS4-61546 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Resistance evolution towards biocides and antimicrobial surfaces N2 - This talk discusses the work at BAM concerning Resistance evolution towards biocides and antimicrobial surfaces. It shows (i) that biocides affect rates of mutation and horizontal gene transfer, (ii) that biocides show heterogeneous killing facilitating tolerance evolution, and (iii) that biocides and antibiotics show strong combination effect on growth and selection. T2 - Exchange seminar with Nottingham Trent University CY - Berlin, Germany DA - 03.09.2024 KW - Antimicrobial resistance KW - Bacteria KW - Standardization KW - Biocides KW - Antimicrobial surfaces PY - 2024 AN - OPUS4-61548 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Altmann, Korinna T1 - Results of the Plastic in the environment comparative test N2 - The talk describes the sturcture and the results of the "plastics in the environment" comparative test of 2019. T2 - Symposium "Challenges of microplastic analysis – Bridging state of the art and policy needs” CY - Online meeting DA - 09.09.2021 KW - Microplastic KW - Comparative test KW - ILC PY - 2021 AN - OPUS4-53236 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Erning, Johann Wilhelm T1 - Risiken in der Trinkwasser-Installation: Das Regelwerk im Kontext von Planung, Bau und Betrieb N2 - Die Herangehensweise zur Bewertung von Risisken in der Trinkwasserinstallation im Zusammenhang mit den Vorgaben der neuen EU-Trinkwasserrihrlin werden diskutiert. Auswirkungen der Veränderungen auf die Wrkstoffauswahl werden gezeigt T2 - Die neue EU-Trinkwasserrichtlinie CY - Online meeting DA - 07.07.2021 KW - Korrosion KW - Trinkwasser-Installation KW - Hygiene PY - 2021 AN - OPUS4-52937 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -