TY - CONF A1 - Raysyan, Anna A1 - Schneider, Rudolf T1 - Lateral Flow ImmunoAssays for the detection of bisphenol A emissions from plastic materials N2 - Bisphenol A (BPA) is a chemical that has been used in the production of plastics for more than 40 years. BPA released from bottles made of polycarbonates has been identified as a potential endocrine disrupting substance. Comprehensive research has been undertaken to test and verify its effect on animals and human beings. Many methods have been proposed for the determination of BPA, in particular, gas and liquid chromatography. The main disadvantages of such approaches are the high cost of the equipment, the significant duration of an analysis, and difficulties in screening a large number of samples. Lateral Flow ImmunoAssay (LFIA) attributes are high throughput, high specificity and sensitivity, as well as low cost and simplicity. An LFIA test strip was developed. This LFIA is based on anti-BPA antibodies (from mouse) conjugated with gold nanoparticles as the marker by passive absorption and covalent coupling, deposited in an elaborate pad. To form control and test zones, anti-mouse antibody and a BVA-BSA conjugate were spotted on the nitrocellulose membrane, respectively. Negative samples are revealed by red lines both in the test and control zones, respectively, whereas positive samples produce a single red line only in the control zone. The measurement range is 0.05 – 23 μg/L, visually detected by the naked eye within 5 minutes. In addition, the intensities at the test line can be read by dedicated lateral flow readers (opTrilyzer® and Cube by opTricon, Berlin) for a more precise and documented determination. T2 - EBS2019 CY - Florence, Italy DA - 18.02.2019 KW - BPA KW - LFIA KW - Plastic PY - 2019 AN - OPUS4-47477 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -