TY - CONF A1 - Tavernaro, Isabella A1 - Chaudhary, A. A1 - Resch-Genger, Ute T1 - Development of amorphous silica particle based reference materials for surface functional group quantification N2 - Functionalized nanomaterials (NM) with their unique size-dependent properties are of increasing relevance for current and future developments in various fields such as medical and pharmaceutical industry, computing, electronics or food and consumer products. The performance and safety of NM are determined by the sum of their intrinsic physicochemical properties.1 Besides other key parameters, the particle surface chemistry, which is largely controlled by the chemical nature and density of functional groups and ligands, must be considered for a better performance, stability, and processibility of NM, as well as their interaction with the environment. Thus, particle standards with well-designed surfaces and methods for functional group quantification can foster the sustainable development of functional and safe(r) NM.2 Here we provide a brief overview of the ongoing research in division Biophotonics to design tailored amorphous silica reference particles with bioanalytically relevant functional groups and ligands, for the development of standardized and validated surface functional group quantification methods. T2 - Workshop NanoRiskSD project CY - Berlin, Germany DA - 09.06.2022 KW - Nanoparticle KW - Surface analysis KW - Silica KW - Fluorescence KW - Assay PY - 2022 AN - OPUS4-55004 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Olbrich, Adelina-Elisa A1 - An Stepec, Biwen Annie A1 - Koerdt, Andrea A1 - Meermann, Björn T1 - Deciphering microbiological influenced corrosion processes on steel with single cell-ICP-ToF-MS N2 - Microbiologically influenced corrosion (MIC) is a highly unpredictable process dictated by the environment, microorganisms, and the respective electron source. Interaction pathways between cells and the metal surface remain unclear. The development of this novel single cell-inductively coupled plasma-time of flight-mass spectrometry analytical method and a MIC-specific staining procedure facilitate the investigation of steel-MIC interactions. With this it is possible to analyze the multi-elemental fingerprint of individual cells. The detection method revealed elemental selectivity for the corrosive methanogenic archaeal strain Methanobacterium-affiliated IM1. The interface between material and environmental analysis thus receives special attention, e.g., when considering MIC on solid steel. Hence, the possible uptake of individual elements from different steel samples is investigated. Results showed the cells responded at a single-cell level to the different types of supplemented elements and displayed the abilities to interact with chromium, vanadium, titanium, cobalt, and molybdenum from solid metal surfaces. The information obtained will be used in the future to elucidate underlying mechanisms and develop possible material protection concepts, thus combining modern methods of analytical sciences with materials research. T2 - Adlershofer Forschungsforum 2022 CY - Berlin, Germany DA - 11.11.2022 KW - Single cell KW - Microbiological influenced corrosion MIC KW - Sc-ICP-ToF-MS KW - Method development PY - 2022 AN - OPUS4-56250 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Frenzel, Olivia A1 - Piechotta, Christian T1 - Identification of Metabolites and Transformation Products of Bisphenols N2 - Bisphenol A (BPA) is widely used in Polycarbonate (PC), Polyacrylic resins (PAR), Polysulfones (PSU), Epoxy resins (ER), and Polyetherimides (PEI). It is also used in recycled Polyvinyl chloride (PVC) [1–3]. These BPA-containing materials have a wide area of application, especially outside. All outdoor applications are exposed to a variety of environmental impacts, like temperature, solar radiation (physical influences), chemical influences (rain or ozone), biological influences (microorganisms), and mechanical influences (hail, sand). These impacts are damaging and aging the material which can be followed by leaching or migration of pollutants like Bisphenol A into the environment. Understanding the fate and behavior of the released pollutants is very important. Therefore, different transformation products of selected Bisphenols will be generated and analyzed. T2 - 33rd International Symposium on Chromatography – ISC 2022 CY - Budapest, Hungary DA - 18.09.2022 KW - Environment KW - Transformation products KW - Bisphenols KW - Metabolites KW - Wastewater PY - 2022 AN - OPUS4-56202 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Olbrich, Adelina-Elisa A1 - An Stepec, Biwen Annie A1 - Koerdt, Andrea A1 - Meermann, Björn T1 - Deciphering microbiological influenced corrosion processes on steel with single cell-ICP-ToF-MS N2 - Microbiologically influenced corrosion (MIC) is a highly unpredictable process dictated by the environment, microorganisms, and the respective electron source. Interaction pathways between cells and the metal surface remain unclear. The development of this novel single cell-inductively coupled plasma-time of flight-mass spectrometry analytical method and a MIC-specific staining procedure facilitate the investigation of steel-MIC interactions. With this it is possible to analyze the multi-elemental fingerprint of individual cells. The detection method revealed elemental selectivity for the corrosive methanogenic archaeal strain Methanobacterium-affiliated IM1. The interface between material and environmental analysis thus receives special attention, e.g., when considering MIC on solid steel. Hence, the possible uptake of individual elements from different steel samples is investigated. Results showed the cells responded at a single-cell level to the different types of supplemented elements and displayed the abilities to interact with chromium, vanadium, titanium, cobalt, and molybdenum from solid metal surfaces. The information obtained will be used in the future to elucidate underlying mechanisms and develop possible material protection concepts, thus combining modern methods of analytical sciences with materials research. References. T2 - Future WiNS CY - Berlin, Germany DA - 07.12.2022 KW - Single cell KW - Microbiological influenced corrosion MIC KW - Sc-ICP-ToF-MS KW - Method development KW - Ir DNA staining approach KW - Carbon steel corrosion PY - 2022 AN - OPUS4-56567 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dinter, Adelina-Elisa A1 - An-Stepec, Biwen A1 - Wurzler, Nina A1 - Özcan Sandikcioglu, Özlem A1 - Koerdt, Andrea A1 - Meermann, Björn T1 - Deciphering corrosion processes of MIC organisms - single cell-ICP-ToF-MS analysis of archaea on solid steels N2 - ICP-ToF (time of flight) MS enables the analysis of the multi-element fingerprint of single cells. The single cell ICP-ToF-MS is used in the presented poster for the analysis of archaea involved in microbiologically influenced corrosion (MIC) of steel. By means of sc-ICP-ToF-MS, the possible uptake of individual elements from the respective steel is investigated - the information obtained will be used in the future to elucidate underlying mechanisms and develop possible material protection concepts. The work combines modern methods of analytical sciences with materials. T2 - SALSA - Make & Measure 2021 CY - Online meeting DA - 16.09.2021 KW - Sc-ICP-ToF-MS KW - Single cell analysis KW - Microbiologically influenced corrosion KW - Archaea KW - Poster presentation PY - 2021 AN - OPUS4-53337 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dondi, C. A1 - Rey, S. A1 - Scholtz, Lena A1 - Tavernaro, Isabella A1 - Resch-Genger, Ute A1 - Larsson-Callerfelt, A-K. A1 - Shaw, M. T1 - Advances in high-resolution imaging of cell cultures and tissues exposed to airborne particles N2 - High-resolution microscopy techniques are fundamental to our ability to investigate and quantify the toxicological effects of airborne particulate pollutants via imaging of in vitro respiratory models exposed to well-characterised particle suspensions and aerosols. However, all imaging methods are limited in their spatio-temporal resolution, information capacity and suitability for minimally invasive time lapse imaging. Many traditional methods are incompatible with the more complex three-dimensional model systems which have potential for greater correlation between in vitro studies and real-world health effects. New techniques can overcome these limits, increasing our ability to understand and measure biological responses to particulate exposure at subcellular, cellular and multicellular scales. We have investigated a variety of microscopy methods for imaging different respiratory models. T2 - International Aerosol Conference (IAC 2022) CY - Athens, Greece DA - 04.09.2022 KW - In vitro imaging KW - Aerosol particles KW - Fluorescence microscopy KW - High-resolution imaging PY - 2022 AN - OPUS4-55694 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kotthoff, Lisa A1 - Schwerdtle, T. A1 - Koch, Matthias T1 - Investigation of ionophore antibiotics and their transformation products by using electrochemistry coupled to LC-MS N2 - Ionophore antibiotics are used to cure and prevent coccidiosis by chicken especially in broiler farming. The residues are found not only in food products (chicken and eggs) but also in the environment (manure, soil or water). In this work the ionophores monensin (MON), salinomycin (SAL), maduramicin (MAD) and lasalocid (LAS) are investigated aiming to study their transformation products (TPs) through biotransformation processes. Biotransformation can be divided into two phases, phase I: oxidation, reduction or hydrolysis and Phase II: conjugation reactions. It is necessary to further examine the biotransformation pathways to determine TPs to be able to detect residues more specifically in different matrices. The technique of electrochemistry (EC) offers the opportunity to simulate biotransformation processes and to generate TPs for further analysis. The combination of EC with liquid chromatography and mass spectrometry (EC-LC-MS) provide a fast and simple tool to separate and determine the EC-generated TPs. The electrochemical flow through cell is coupled to the (LC)-MS system, allowing the reaction mixture to be separated by a RP-18 column and then analyzed in the MS. The oxidation products are generated at different potentials between 0.0 – 2.5 V vs. Pd/H2 using glassy carbon or boron doped diamond as working electrode materials . The results show a broad spectrum of different TPs depending on used solvents and working electrode materials. Among the generated TPs already known as well as unknown TPs of the drugs can be found. Further investigations on structure elucidation of unkown TPs are planned. T2 - World Conference on Analytical and Bioanalytical Chemistry CY - Barcelona, Spain DA - 23.07.2018 KW - Transformation Product KW - Electrochemistry KW - Ionophore Antibiotics PY - 2018 AN - OPUS4-45600 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Eichhorn, Maria A1 - Eggers, Romina A1 - Ostermann, Markus T1 - XRF as a Sensor on a Mobile Platform for Soil Mapping N2 - The need for sustainable and effective usage of limited resources like water and soil becomes more and more important due to increasing populations and their need for food. The research initiative BonaRes funded by the Federal Ministry of Education and Research, develops strategies to use soil as a sustainable resource for the bio-economy. One part of this initiative is the Intelligence for Soil (I4S) project. It deals with the development of an integrated system for the site-specific soil fertility management. The goal of the project is to establish a mobile sensor platform for soil mapping as well as to apply soil and crop models to deal with the high amount of produced data. These models are going to be used to formulate recommendations on the use of fertilizers and to gain an improvement on the soil fertility. Using this information, the environmental pollution with substances like nitrate contained in fertilizers can be decreased. Furthermore, the costs for farmers can be decreased if only the amounts of fertilizer needed are applied to the field. The task for BAM is installation and optimization of a handheld XRF sensor for online measurement directly on the field. It is a non-destructive technique and quite suitable for an online approach due to a low maintenance in sample preparation and a fast and simultaneous multi element analysis. The elements of interest in this case are light elements like phosphorus, calcium, and potassium. One of the major tasks is the calibration of the sensor for different soils is finding suitable calibration models, which are evolved to deal with different types of soil. Due to the multi element analysis, micro and macro nutrients can be determined simultaneously. Those differ a lot in their contents, which makes it inevitable to optimize and adapt the analytical procedure. By analyzing soil it is always important to deal with matrix effects, that is why the influence of different disturbances like the moisturize content and the grain size needs to be investigated. The XRF is only one of many sensors that combined give customized fertilizing recommendations in the end. T2 - Tag der Chemie CY - TU Berlin, Germany DA - 11.07.2019 KW - XRF KW - BonaRes PY - 2019 AN - OPUS4-49551 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Eichhorn, Maria A1 - Eggers, Romina A1 - Ostermann, Markus T1 - Development of an online XRF method to determine micro- and macro nutrients in soil N2 - The need for sustainable and effective usage of limited resources like water and soil becomes more and more important due to increasing populations and their need for food. The research initiative BonaRes funded by the Federal Ministry of Education and Research, develops strategies to use soil as a sustainable resource for the bio-economy. One part of this initiative is the Intelligence for soil (I4S) project. It deals with the development of an integrated system for the site-specific soil fertility management. The goal of the project is to establish a mobile sensor platform for soil mapping as well as to apply soil and crop models to deal with the high amount of produced data. These models are going to be used to formulate recommendations on the use of fertilizers and to gain an improvement on the soil fertility. Using these information’s, the environmental pollution with substances like nitrate contained in fertilizers can be decreased. Furthermore, the costs for farmers can be decreased if only the amounts of fertilizer actually needed are applied to the field. The task for the Federal Institute for Materials Research and Testing (BAM) is the installation and optimization of a handheld XRF sensor for the online measurement directly on the field. It is a non-destructive technique and quite suitable for the online approach due to a low maintenance in the sample preparation and a fast and simultaneous multi element analysis. The elements of interest in this case are light elements like phosphorus, calcium and potassium. One of the major tasks is the calibration of the sensor for different soils, therefore, calibration models are evolved to deal with different types of soil. Due to the multi element analysis, micro and macro nutrients can be determined simultaneously. Those differ a lot in their contents which makes it inevitable to optimize and adapt the measuring procedure. By analyzing soil, its always important to deal with matrix effects, that is why the influence of different disturbances like the moisturize content and the grain size needs to be investigated. The XRF is only one of many sensors that combined give customized fertilizing recommendations in the end. T2 - CANAS 2019 CY - Freiberg, Germany DA - 24.09.2019 KW - XRF KW - BonaRes PY - 2019 AN - OPUS4-49553 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Raysyan, Anna A1 - Savvina, Olga T1 - Determination of mycotoxins in food and pharmaceuticals by immunochemical analysis N2 - Mycotoxins are toxic secondary metabolites produced by filamentous fungi. The contamination of mycotoxins in food and feed products has been recognized to be an important safety issue for human and animal health. In the context of food safety, medical herbs nowadays are also considered as potential source of mycotoxins. Considering that mycotoxins are generally stable compounds and could not be destroyed completely during food-processing operations, prevention of contamination is identified as main issue. Currently, biochemical, and immunochemical methods based on the use of antibodies as a specific recognition bioreagents are increasingly found to be applied in screening. Along with good common enzyme linked immunosorbent assay (ELISA), more and more widely used method is fluorescence polarization immunoassay (FPIA). These methods help to monitor of many toxic substances in large number of samples and carried out quickly, easily, cheaply, and give good results in quantifying the one or more substances. That is why the use of immunochemical methods of analysis, such as ELISA and FPIA, is the most promising for solving this problem. Thus, the aim of our work is to optimize the determination of mycotoxins by ELISA and FPIA in medical herbs. We selected antibodies and a tracer for the FPIA, then we constructed obtained a calibration curve and determined the sensitivity of this method for the samples in aqueous media. Then, the optimal sample preparation was elaborated: for spiked nuts samples were selected and prepared and a calibration curve was obtained with respect to the matrix effect. After that, the analysis of spiked nuts samples was performed by ELISA and FPIA and the sensitivity of both methods was compared. T2 - Global Food Science Student Competition CY - Jiangnan University, Wuxi, China DA - 14.11.2018 KW - Mycotoxins KW - FPIA KW - ELISA PY - 2018 AN - OPUS4-50048 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -