TY - CONF A1 - Ecke, Alexander T1 - Magnetpartikelbasierte Immunoassays für die Online-Trinkwasseranalyik N2 - Wir berichten hier über die Entwicklung plattenbasierter Magnetpartikel-Immunoassays für die Bestimmung des Breitbandantibiotikums Amoxicillin und des nicht-steroidalen Antirheumatikums Diclofenac im Trinkwasser. Für die beiden Analyten wurden unterschiedliche Ansätze entwickelt, mit partikelgebundenen Antikörpern für AMX und Analyt-gekoppelten Partikeln für DCF, die die simultane Detektion beider Kontaminanten im nanomolaren Konzentrationsbereich erlauben. T2 - Interdisziplinäres Doktorandenseminar des AK Prozessanalytik CY - Online meeting DA - 21.09.2020 KW - Magnetpartikel KW - Immunoassay KW - Trinkwasseranalytik KW - Diclofenac KW - Amoxicillin PY - 2020 AN - OPUS4-51345 LA - mul AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - Immunanalytische Bestimmung von β-Lactam-Antibiotika und deren Hydrolyseprodukten im Trinkwasser N2 - Die speziellen Herausforderungen der immunanalytischen Bestimmung von β-Lactam-Antibiotika in (Trink-)Wasserproben werden anhand eines neu entwickelten ELISA (enzyme-linked immunosorbent assay) für das Breitbandantibiotikum Amoxicillin diskutiert. Es konnte gezeigt werden, dass die Hydrolyse des Analyten einen positiven Einfluss auf die Erkennung durch den Antikörper hat. Das entsprechende Hydrolyseprodukt, zu dem der Antikörper folglich eine höhere Affinität aufweist, wurde identifiziert und ein entsprechendes Verhalten wurde auch für andere β-Lactam-Antibiotika aus der Gruppe der Penicilline beobachtet. Eine Strategie für die Analyse von Wasserproben hinsichtlich Kontamination mit den hier betrachteten β-Lactamen und deren Hydrolyseprodukten wurde ausgearbeitet. T2 - Wasser 2020 - Jahrestagung der Wasserchemischen Gesellschaft CY - Meeting was canceled DA - 18.05.2020 KW - ELISA KW - Immunoassay KW - Antibiotika KW - Hydrolyseprodukte KW - Amoxicillin PY - 2020 UR - https://www.wasserchemische-gesellschaft.de/images/pdf_veranstaltungen/2020/Wasserchemische_Gesellschaft_Tagungsband_Wasser_2020_s.pdf SP - 466 EP - 470 AN - OPUS4-51061 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Almeida, Â. A1 - Calisto, V. A1 - Esteves, V. A1 - Schneider, Rudolf A1 - Figueira, E. A1 - Soares, A. A1 - Freitas, R. T1 - Can ocean warming alter sub-lethal effects of antiepileptic and antihistaminic pharmaceuticals in marine bivalves? N2 - The negative effects induced in marine organisms by Climate Change related abiotic factors consequences, namely ocean warming, are well-known. However, few works studied the combined impacts of ocean warming and contaminants, as pharmaceutical drugs. Carbamazepine (CBZ) and cetirizine (CTZ) occur in the marine environment, showing negative effects in marine organisms. This study aimed to evaluate the impacts of Ocean warming on the effects of CBZ and CTZ, when acting individually and combined (drug vs drug), in the edible clam Ruditapes philippinarum. For that, drugs concentration, bioconcentration factors and biochemical parameters, related with clam’s metabolic capacity and oxidative stress, were evaluated after 28 days exposure to environmentally relevant scenarios of these stressors. The results showed limited impacts of the drugs (single and combined) at control and warming condition. Indeed, it appeared that warming improved the oxidative status of contaminated clams (higher reduced to oxidized glutathione ratio, lower lipid peroxidation and Protein carbonylation levels), especially when both drugs were combined. This may result from clam’s defence mechanisms activation and reduced metabolic capacity that, respectively, increased elimination and limited production of reactive oxygen species. At low stress levels, defence mechanisms were not activated which resulted into oxidative stress. The present findings highlighted that under higher stress levels clams may be able to activate defence strategies that were sufficient to avoid cellular damages and loss of redox homeostasis. Nevertheless, low concentrations were tested in the present study and the observed responses may greatly Change under increased pollution levels or temperatures. Further research on this topic is needed since marine heat waves are increasing in frequency and intensity and pollution levels of some pharmaceuticals are also increasing in coastal systems. KW - Klimaerwärmung KW - Meer KW - Antiepileptika KW - Antihistaminika KW - Muscheln KW - Immunoassay KW - ELISA KW - Carbamazepine KW - Cetirizine PY - 2021 DO - https://doi.org/10.1016/j.aquatox.2020.105673 SN - 0166-445X VL - 230 SP - 105673 PB - Elsevier B.V. AN - OPUS4-51840 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Immunoanalytical platforms for on-site environmental health and food safety testing N2 - An overview of methods developed in the division. T2 - FoodSmartphone.eu - Online Symposium CY - Online meeting DA - 25.11.2020 KW - Umweltanalytik KW - Lebensmittelanalytik KW - Immunoassay KW - Biosensor PY - 2020 AN - OPUS4-51841 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Raysyan, Anna A1 - Schneider, Rudolf T1 - Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products N2 - One of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A. However, despite the large number of studies on the toxicity and hormonal activity of BPA, there are still open questions and thus considerable media attention regarding BPA toxicity. Hence, it is necessary to develop a sensitive, simple, cost-efficient, specific, portable, and rapid method for monitoring bisphenol A and for high sample throughput and on-site screening analysis. Lateral flow immunoassays have potential as rapid tests for on-site screening. To meet sensitivity criteria, they must be carefully optimized. A latex microparticle-based LFIA for detection of BPA was developed. The sensitivity of the assay was improved by non-contact printing of spot grids as the control and test lines with careful parameter optimization. Results of the test could be visually evaluated within 10 min with a visual cut-off of 10 µg/L (vLOD). Alternatively, photographs were taken, and image analysis performed to set up a calibration, which allowed for a calculated limit of detection (cLOD) of 0.14 µg/L. The method was validated for thermal paper samples against ELISA and LC–MS/MS as reference methods, showing good agreement with both methods KW - Endokrine Disruptoren KW - Polymere KW - Schnelltest KW - Endocrine disruptor KW - Bisphenol A KW - LFIA KW - Immunoassay PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-540492 DO - https://doi.org/10.3390/bios11070231 VL - 11 IS - 7 SP - 1 EP - 11 PB - MDPI CY - Basel AN - OPUS4-54049 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, Rudolf T1 - Portable, antikörperbasierte Analysenverfahren für die Schadstofferfassung im Wasserkreislauf N2 - Die Wasseranalytik wird bestimmt von zwei Hauptbedarfen, einerseits der Erfassung der Gewässergüte und Grundwassergüte als Basis für die Bereitstellung einwandfreien Trinkwassers und zum anderen des Monitorings der Wasserqualität in den Prozessen der Wasseraufbereitung und der Abwasserreinigung. Für beide Anwendungsbereiche ist eine laborbasierte Analytik eher hinderlich, werden die Analysenergebnisse doch eigentlich vor Ort benötigt, um etwa Beprobungskampagnen rasch anpassen zu können. Des Weiteren werden die Daten auch schnell benötigt, um zeitnah in die technischen Prozesse einzugreifen. Organische Kontaminanten werden meist über chromatographische Verfahren, häufig gekoppelt mit einer massenspektrometrischen Detektion, in instrumentellen Labormethoden erfasst, und die hochauflösende Massenspektrometrie und die Non-target-Analytik haben viel zum Wissen über das Vorkommen, insbesondere von sogenannten „Emerging Contaminants“, beigetragen, sowie zum Verständnis der beteiligten Eintragspfade und Abbauwege. Moderne, portable Analysenmethoden und insbesondere Sensoren werden zukünftig aber ihren Platz in der Schadstofferfassung im Wasserkreislauf einnehmen. Dies gilt insbesondere für antikörperbasierte Analysenverfahren, da erst diese oft die nötige Selektivität und Sensitivität in die Detektionsmethoden einbringen. KW - Biosensoren KW - Schnelltests KW - Immunoassay KW - Abwasser PY - 2021 DO - https://doi.org/10.1002/vomw.202100005 VL - 119 IS - 2 SP - 35 EP - 53 PB - Wiley-VCH GmbH CY - Weinheim AN - OPUS4-54050 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Portable, antikörperbasierte Analysenverfahren für die Schadstofferfassung im Wasserkreislauf N2 - Die Wasseranalytik wird bestimmt von zwei Hauptbedarfen, einerseits der Erfassung der Gewässergüte und Grundwassergüte als Basis für die Bereitstellung einwandfrei-en Trinkwassers und zum anderen des Monitorings der Wasserqualität in den Prozes-sen der Wasseraufbereitung und der Abwasserreinigung. Für beide Anwendungsbe-reiche ist eine laborbasierte Analytik eher hinderlich, werden die Analysenergebnisse doch eigentlich vor Ort benötigt, um etwa Beprobungskampagnen rasch anpassen zu können. Desweiteren werden die Daten auch schnell benötigt, um zeitnah in die technischen Prozesse einzugreifen. Organische Kontaminanten werden meist über chromatographische Verfahren, häufig gekoppelt mit einer massenspektrometrischen Detektion, in instrumentelle Labormethoden erfasst und die hochauflösende Massen-spektrometrie und die Non-target-Analytik haben viel zum Wissen über das Vorkom-men, insbesondere von sog. „emerging contaminants“, beigetragen, sowie zum Ver-ständnis der beteiligten Eintragspfade und Abbauwege. Moderne, portable Analy-senmethoden und insbesondere Sensoren werden zukünftig aber ihren Platz in der Schadstofferfassung im Wasserkreislauf einnehmen. Dies gilt insbesondere für anti-körperbasierte Analysenverfahren, da erst diese oft die nötige Selektivität und Sensitivität in die Detektionsmethoden einbringen. T2 - Wasser 2021 - Jahrestagung der Wasserchemischen Gesellschaft in der GDCh CY - Online meeting DA - 10.05.2021 KW - Immunoassay KW - Biosensoren KW - Schnelltest KW - Elektrochemisch PY - 2021 AN - OPUS4-54051 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Environmental monitoring by high-throughput immunoanalytical methods and portable devices N2 - A vast number of emerging pollutants has been detected in the environment over the last decades. Analytical methods suitable for trace analysis are needed that are desirably also fast, inexpensive and, if possible, robust and portable. Immunoanalytical, i.e. antibody-based, methods which are available in a broad range of formats, can be profitably used here to analyse for the distribution and the trends of concentration levels of contaminants in the environment. Some of these formats are single-analyte but high-throughput methods. In order to use them wisely, indicator substances, sometimes called anthropogenic markers, should be selected and used in screening approaches. Other methods are suitable to be performed on portable instrumentation in the field (on-site) or in facilities such as wastewater treatment plants for on-line monitoring. Furthermore, there are array technologies that allow for parallel (multiplex) analysis of several analytes of interest. The microtiter-plate based ELISA (Enzyme-linked Immunosorbent Assay) is the method of choice for the analysis of a large number of samples [1]. ELISAs are available to monitor for anthropogenic markers such as the antiepileptic carbamazepine, the analgesic diclofenac, the antihistaminic cetirizine, the steroid hormone estrone, the antimicrobial sulfamethoxazole, the stimulants caffeine and cocaine, the priority pollutant bisphenol A, and the bile acid isolithocholic acid. For on-site screening and monitoring, simpler formats, like mix-and-read assays, e.g. the Fluorescence Polarization Immunoassay (FPIA) [2] or Lateral-flow Immunoassays (LFIA) [3] are more suitable tools, the latter based on dipsticks or cassettes, that is why they are also called pregnancy test-like assays. The suitability of multi-analyte formats such as immunomicroarrays depends on the choice of a signal-producing system that provides small uncertainties and good reproducibility of the measurements. Bead-based (“suspension”) arrays read out in flow cytometers are a powerful platform for multiplex assays [4]. Electrochemical formats run on portable devices provide additional advantages as no light source is required. They are most promising for stand-alone analysers and biosensors T2 - European Biosensor Symposium 2021 CY - Online meeting DA - 09.03.2021 KW - Biosensoren KW - Schnelltest KW - Elektrochemisch KW - Immunoassay PY - 2021 AN - OPUS4-54052 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ecke, Alexander A1 - Bohm, Konrad A1 - Schneider, Rudolf T1 - Magnetpartikelbasierte Immunoassays als vielseitiges Werkzeug für die Umweltanalytik N2 - Der Bedarf nach schnellen und kostengünstigen Analysemethoden in der (Trink-)Wasseranalytik steigt mit dem zunehmenden Eintrag pharmazeutisch aktiver Substanzen in die Umwelt. Insbesondere die Kontamination mit Antibiotika erscheint neben ökotoxikologischen Effekten vor allem in Hinblick auf die Evolution resistenter Keime im Wasser besorgniserregend. Wir berichten hier über die Entwicklung eines magnetpartikelbasierten Immunoassays (MBBA) zur Detektion des Breitbandantibiotikums Amoxicillin (AMX) in Wasserproben, der im Vergleich zum entsprechenden Enzyme-linked Immunosorbent Assay (ELISA) eine wesentlich kürzere Analysendauer sowie einen breiteren Messbereich mit niedrigerer Nachweisgrenze aufweist. Das angewendete Assayprinzip ist dabei leicht auf weitere Analyten übertragbar und ermöglicht die Implementierung in einen Immunosensor. KW - Magnetpartikel KW - Amoxicillin KW - Immunoassay KW - Wasser KW - Antikörper PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-527366 UR - https://www.gdch.de/netzwerk-strukturen/fachstrukturen/umweltchemie-und-oekotoxikologie/publikationen/mitteilungen/archiv/2021.html SN - 1618-3258 VL - 27 IS - 2 SP - 48 EP - 51 PB - Gesellschaft Deutscher Chemiker (GDCh) CY - Frankfurt/Main AN - OPUS4-52736 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ecke, Alexander A1 - Schneider, Rudolf T1 - Pitfalls in the Immunochemical Determination of β-Lactam Antibiotics in Water N2 - Contamination of waters with pharmaceuticals is an alarming problem as it may support the evolution of antimicrobial resistance. Therefore, fast and cost-effective analytical methods for potential on-site analysis are desired in order to control the water quality and assure the safety of its use as a source of drinking water. Antibody-based methods, such as the enzyme-linked immunosorbent assay (ELISA), can be helpful in this regard but can also have certain pitfalls in store, depending on the analyte. As shown here for the class of β-lactam antibiotics, hydrolysis of the β-lactam ring is a key factor in the immunochemical analysis as it influences antibody recognition. With the antibody used in this study, the limit of detection (LOD) in the immunoassay could be significantly reduced by hydrolysis for the five tested penicillins, with the lowest LOD for carbenicillin (0.2 nmol/L) and the greatest impact on penicillins G and V (reduction by 85%). In addition to enhanced quantification, our strategy also provides access to information about the degree of hydrolysis in water samples as shown for the most abundant penicillin amoxicillin. KW - ELISA KW - Immunoassay KW - β-Lactam KW - Amoxicillin KW - Hydrolysis PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-522832 DO - https://doi.org/10.3390/antibiotics10030298 SN - 2079-6382 VL - 10 IS - 3 SP - 298 PB - MDPI CY - Basel, CH AN - OPUS4-52283 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander T1 - Magnetic Bead-Based Immunoassays for Online Sensing Applications N2 - The demand for quick and reliable online sensors is rising not only in the field of environmental analysis with new and diversifying sources of pollution. A major aspect of this is the contamination of (drinking) water with pharmaceuticals representing a potential health threat and requiring timely countermeasures in case of contamination. In this regard, antibody-based methods can be helpful as they enable fast and cost-effective on-site and potentially online analyses. The transition from the conventional plate-based formats like ELISA (enzyme-linked immunosorbent assay) to an immunosensor fit for online sensing can be achieved by using magnetic microparticles as a mobile sensing platform. Beads are prepared by either coupling the antibody or the analyte to the surface. Both these strategies are presented using the example of two relevant contaminants in water: diclofenac (DCF) & amoxicillin (AMX), and can be transferred to other analytes. The developed magnetic bead-based assays (MBBAs) exhibit lower LODs, enhanced sensitivity as well as shorter time of analysis than the respective ELISAs and can be integrated into an immunosensor with electrochemical detection on a microfluidic chip. T2 - European Biosensor Symposium 2021 CY - Online meeting DA - 09.03.2021 KW - Immunoassay KW - Magnetic Beads KW - Diclofenac KW - Amoxicillin KW - Online Sensing PY - 2021 AN - OPUS4-52285 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander T1 - Magnetic Bead-Based Immunoassays for Online Drinking Water Analysis N2 - We present the development of magnetic bead-based immunoassays for two potential contaminants of drinking water: the nonsteroidal anti-inflammatory drug diclofenac and the broad-spectrum antibiotic amoxicillin. For these analytes, different assay formats were developed which can generally be transferred to other analytes. The use of magnetic beads not only enables implementation of the assays into an online system since the beads can be reversibly immobilized by applying a magnetic field. Furthermore, our MBBAs hold several advantages over the conventional ELISAs (enzyme-linked immunosorbent assays) for both analytes, such as lower limits of quantification, shorter assay duration and less washing steps required. Strategies for the setup and miniaturization of an online immunosensor including electrochemical readout on a microfluidic chip will be discussed. T2 - JCF-Frühjahrssymposium CY - Online Meeting DA - 29.03.2021 KW - Magnetic Beads KW - Immunoassay KW - Immunosensor KW - Diclofenac KW - Amoxicillin PY - 2021 AN - OPUS4-52426 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander T1 - Magnetpartikelbasierte Immunoassays für die Bestimmung von Amoxicillin und Diclofenac im Trinkwasser N2 - Wir berichten hier über die Entwicklung von magnetpartikelbasierten Immunoassays für die Bestimmung zweier relevanter Kontaminanten des Trinkwassers: Diclofenac (DCF) und Amoxicillin (AMX). Für die beiden Analyten wurden die Assays nach unterschiedlichen Prinzipien entwickelt, die sich auch auf weitere Analyten übertragen lassen. Im Vergleich zum herkömmlichen ELISA weisen die hier entwickelten magnetpartikelbasierten Assays einen größeren Messbereich mit niedrigeren Bestimmungsgrenzen bei gleichzeitig deutlich reduzierter Analysendauer auf. Darüber hinaus können die Immunoassays in ein automatisierbares Onlinesystem zur Analyse in der Trinkwasserversorgung implementiert werden. Strategien hierfür werden erläutert. T2 - Spurenstoffe und Krankheitserreger im Wasserkreislauf CY - Online Meeting DA - 19.04.2021 KW - Immunoassay KW - Amoxicillin KW - Diclofenac KW - Immunosensor KW - Magnetpartikel PY - 2021 AN - OPUS4-52466 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander T1 - Magnetpartikelbasierte Immunoassays für die Online-Trinkwasseranalytik N2 - Die zunehmende Kontamination von (Trink)-Wasser mit pharmazeutischen Rückständen stellt ein potenzielles Gesundheitsrisiko dar und verlangt nach schnellen und zuverlässigen Analysemethoden. Hierbei können antikörper-basierte Methoden helfen, da sie schnelle und kostengünstige Vor-Ort-Messungen sowie Onlineanalysen ermöglichen. Der Übergang von konventionellen plattenbasierten Formaten, wie ELISA (Enzyme-linked Immunosorbent Assay), zu einem Immunosensor für Online-Messungen wird durch den Einsatz magnetischer Mikropartikel als mobile Messplattform ermöglicht. Die Partikel werden dazu entweder mit dem Antikörper oder dem Analyten gekoppelt. Diese beiden Strategien werden am Beispiel zweier relevanter Kontaminanten des Wassers gezeigt: Diclofenac (DCF) & Amoxicillin (AMX), und sind leicht auf andere Analyten übertragbar. Die magnetpartikelbasierten Assays (MBBAs) weisen niedrigere Nachweisgrenzen, breitere Messbereiche sowie kürzere Analysezeiten als die entsprechenden ELISAs auf und können in einen Immunosensor mit elektrochemischer Detektion auf einem mikrofluidischen Chip integriert werden. T2 - Wasser 2021 CY - Online meeting DA - 10.05.2021 KW - Immunoassay KW - Immunosensor KW - Magnetpartikel KW - Amoxicillin KW - Diclofenac PY - 2021 AN - OPUS4-52640 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Carl, Peter T1 - Partikelbasierte Multianalyt- Fluoreszenzimmunoassays für die Unweltanalytik und Biotechnologie N2 - Zur Beurteilung der Wasserqualität können Markersubstanzen, wie Carbamazepin, Diclofenac, Koffein und Gallensäuren, verwendet werden. Erhöhte Konzentrationen dieser anthropogenen Marker weisen auf Einleitstellen von Abwasser hin und zeigen somit an, wo Gewässer verschmutzt werden. Diese Arbeit beschreibt deshalb die Etablierung, Optimierung und Validierung eines partikelbasierten Suspensionsarray Fluoreszenzimmunoassays (SAFIA), mit dem diese Substanzen gleichzeitig und parallel in vielen Proben quantitativ bestimmt werden können. Für SAFIA wurde das Format des kompetitiven indirekten Immunoassays gewählt. Als Plattform wurden fluoreszenzintensitätscodierte Polystyrol-Kern/Siliziumdioxid-Schale-Partikel, auf deren Oberfläche Haptene als kompetitive Bindungsstellen für Antikörper immobilisiert wurden, verwendet. Hier zeigte sich, dass eine selektive Erkennung der Haptenstruktur auf der Oberfläche der Partikel durch die Antikörper nur gegeben war, wenn die Partikel zusätzlich mit Polyethylenglykolgruppen funktionalisiert wurden. Zum Auslesen des Fluoreszenzsignals wurde ein Durchflusszytometer verwendet. Kompatibilität mit der Durchführung in Mikrotiterplatten wurde erreicht, indem eine Stopp-lösung für den SAFIA, basierend auf Formaldehyd, entwickelt wurde. Nach der Optimierung der Assayparameter können die oben genannten vier Analyten gleichzeitig in einer Probe mindestens bis zu einer Konzentration von 0.3 μg L-1 nachgewiesen werden. Der Assay kann ohne Wasch-schritte ausgeführt werden und ist somit einfacher als konventionelle Immunoassays, wie z. B. ELISA. Der SAFIA wurde hinsichtlich Interferenzen und Selektivität untersucht. Dabei zeigte sich, dass Matrixbestandteile, die in wässrigen Umweltproben vorkommen, keinen bzw. nur einen ge-ringen Einfluss auf den SAFIA hatten. Im simulierten Umweltscreening eines Flusses und mithilfe der Analyse von Abwasserproben wurde SAFIA validiert; hier zeigte SAFIA eine mit ELISA vergleich-bare Genauigkeit, bei gleichzeitiger Senkung der Analysenzeit und -kosten. Die gleichzeitige Detektion mehrerer anthropogener Marker erlaubte zudem Rückschlüsse auf die Art von Verschmutzungsquellen, im Gegensatz zu ELISA, mit dem nur ein Analyt bestimmt werden kann. Da hochaffine Antikörper für Immunoassays essenziell sind, wurde ein SAFIA zum Screening antikörperproduzierenden Zellen (Hybridomzellen) entwickelt und eingesetzt. Durch die Implementierung eines Sandwichimmunoassays zur IgG Bestimmung und durch Verwendung von homo- und heterologen Haptenstrukturen konnten aus einem Pool von Hybridomzellen diejenigen ausgewählt werden, deren sekretierter Antikörper die geringsten Nachweisgrenzen im Immunoassay erlauben. Damit konnte das Anwendungsspektrum des SAFIA maßgeblich vergrößert werden. Er vereinfacht als biotechnologisches Werkzeug stark sowohl die Produktion von monoklonalen Antikörpern als auch die Analyse von Umweltproben. T2 - Wasser 2021 Jahrestagung der Wasserchemischen Gesellschaft CY - Online Meeting DA - 10.05.2021 KW - SAFIA KW - Anthropogene Marker KW - Immunoassay KW - Partikel KW - Isolithocholsäure KW - Screening KW - Carbamazepin KW - Diclofenac KW - Coffein PY - 2021 AN - OPUS4-52629 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ecke, Alexander A1 - Westphalen, Tanja A1 - Hornung, J. A1 - Voetz, M. A1 - Schneider, Rudolf T1 - A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac N2 - Increasing contamination of environmental waters with pharmaceuticals represents an emerging threat for the drinking water quality and safety. In this regard, fast and reliable analytical methods are required to allow quick countermeasures in case of contamination. Here, we report the development of a magnetic bead-based immunoassay (MBBA) for the fast and cost-effective determination of the analgesic diclofenac (DCF) in water samples, based on diclofenac-coupled magnetic beads and a robust monoclonal anti-DCF antibody. A novel synthetic strategy for preparation of the beads resulted in an assay that enabled for the determination of diclofenac with a significantly lower limit of detection (400 ng/L) than the respective enzyme-linked immunosorbent assay (ELISA). With shorter incubation times and only one manual washing step required, the assay demands for remarkably shorter time to result (< 45 min) and less equipment than ELISA. Evaluation of assay precision and accuracy with a series of spiked water samples yielded results with low to moderate intra- and inter-assay variations and in good agreement with LC–MS/MS reference analysis. The assay principle can be transferred to other, e.g., microfluidic, formats, as well as applied to other analytes and may replace ELISA as the standard immunochemical method. KW - Immunoassay KW - Magnetic beads KW - Diclofenac KW - Water analysis KW - LC-MS/MS PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-542346 DO - https://doi.org/10.1007/s00216-021-03778-7 SN - 1618-2650 VL - 414 SP - 1563 EP - 1573 PB - Springer CY - Heidelberg AN - OPUS4-54234 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Advanced Materials in antibody-based analytical techniques for environmental analysis N2 - Antibody-based analytical techniques have gained increasing importance in environmental analysis. Rapid assays, portable devices and formats that require a limited number of steps have a high potential for on-site analysis in outdoor environments or in environmentally relevant facilities such as wastewater treatment plants. Many of these methods have become more sensitive and more versatile due to new, advanced materials, such as novel particles, specifically prepared surfaces, new labels, specifically coated electrodes etc. The talk shows some of our works in this context. T2 - V Analytical Chemistry Symposium: Advanced Materials for Environmental Applications CY - Online meeting DA - 24.11.2021 KW - Biosensoren KW - Immunoassay KW - Schnelltest KW - Vor-Ort-Analytik KW - Portable Analytik PY - 2021 AN - OPUS4-54038 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, Rudolf A1 - Almeida, Â. A1 - Calisto, V. A1 - Esteves, V. I. A1 - Soares, A. M. V. M. A1 - Freitas, R. T1 - Salinity-dependent impacts on the effects of antiepileptic and antihistaminic drugs in Ruditapes philippinarum N2 - In Coastal Systems, pollutants as pharmaceutical drugs exert changes from the molecular to the organism level in marine bivalves. Besides pollutants, Coastal Systems are prone to changes in environmental Parameters, as the alteration of salinity values because of Climate Change. Together, these Stressors (pharmaceutical drugs and salinity changes) can exert different threats than each Stressor acting individually; for example, salinity can change the physical-chemical properties of the drugs and/or the sensitivity of the organisms to them. However, limited Information is available on this subject, with variable results, and for this reason, this study aimed to evaluate the impacts of salinity changes (15,25 and 35) on the effects of the antiepileptic carbamazepine (CBZ, 1 (ig/L) and the antihistamine cetirizine (CTZ, 0.6 pg/L), when acting individually and combined (CBZ + CTZ), in the edible clam Ruditapes philippinarum. After 28 days ofexposure, drugs concentrations, bioconcentration factors and biochemical parameters, related to clam's metabolic caparity and oxidative stress were evaluated. The results showed that dams under low salinity suffered more changes in metabolic, antioxidant and biotransformation activities, in comparison with the remaining salinities under study. However, limited impacts were observed when comparing drug effects at low salinity. Indeed, it seemed that CTZ and CBZ + CTZ, under high salinity (salinity 35) were the worst exposure conditions for the dams, since they caused higher leveis of cellular damage. It Stands out that salinity changes altered the impact of pharmaceutical drugs on marine bivalves. KW - Muscheln KW - Salinität KW - Carbamazepin KW - Cetirizin KW - ELISA KW - Immunoassay KW - Antiepileptikum PY - 2022 DO - https://doi.org/10.1016/j.scitotenv.2021.150369 SN - 1879-1026 VL - 806 SP - 1 EP - 13 PB - Elsevier Science CY - Amsterdam AN - OPUS4-55561 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Almeida, Â. A1 - Calisto, V. A1 - Esteves, V. I. A1 - Schneider, Rudolf A1 - Soares, A. M. V. M. A1 - Freitas, R. T1 - Responses of Ruditapes philippinarum to contamination by pharmaceutical drugs under ocean acidification scenario N2 - In coastal systems, organisms are exposed to amultitude of stressors whose interactions and effects are poorly studied. Pharmaceutical drugs and Climate Change consequences, such as lowered pH, are examples of stressors affecting marine organisms, as bivalves. Although a vast literature is available for the effects of these stressors when acting individually, very limited information exists on the impacts that the combination of both can have on marine bivalves. For this reason, this study aimed to evaluate the impacts of a simulated ocean acidification scenario (control pH, 8.0; lowered pH, pH 7.6) on the effects of the antiepileptic carbamazepine (CBZ, 1 μg/L) and the antihistamine cetirizine (CTZ, 0.6 μg/L), when acting individually and combined (CBZ + CTZ), on the edible clam Ruditapes philippinarum. After 28 days of exposure, drug concentrations, bioconcentration factors and biochemical parameters related to the clams' metabolic capacity and oxidative stress were evaluated. The results showed that R. philippinarum clams responded differently to pharmaceutical drugs depending on the pH tested, influencing both bioconcentration and biological responses. In general, drug combined treatments showed fewer impacts than drugs acting alone, and acidification seemed to activate at a higher extension the elimination processes that were not activated under control pH. Also, lowered pH per se exerted negative impacts (e.g., cellular damage) on R. philippinarum and the combination with pharmaceutical drugs did not enhance the toxicity. KW - Biosensoren KW - Immunoassay KW - ELISA KW - Vor-Ort-Analytik KW - Toxikologie KW - Pharmaceutical drugs KW - Bivalves KW - Ocean acidification KW - Biomarkers KW - Climate change PY - 2022 DO - https://doi.org/10.1016/j.scitotenv.2022.153591 SN - 1879-1026 VL - 824 SP - 1 EP - 11 PB - Elsevier Science CY - Amsterdam AN - OPUS4-55590 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Insights at the point-of-need via immunoanalytical methods N2 - A vast number of emerging pollutants is being detected in the environment. Another lingering problem are health-threatening contaminants, such as mycotoxins, that deteriorate food and feed, and the pathogens themselves. Analytical methods, suitable for trace analysis, are needed that are desirably also fast, inexpensive and, if possible, robust and portable. It is set out, how immunoana-lytical, i.e., antibody-based methods, which are elaborated in a broad range of formats, can be profitably used to gain insights on the distribution and concentration trends of the target analytes at the point-of-need. T2 - Kolloquium der Fakultät für Pharmazie der Universität Porto CY - Porto, Portugal DA - 01.07.2022 KW - Immunoassay KW - Sensor KW - Antibody PY - 2022 AN - OPUS4-56781 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Advanced materials broadening the scope of antibody-based analytical methods N2 - Immunoanalytical Techniques, i.e., antibody-based analytical methods, have been used for decades in clinical diagnostics. What makes them attractive for other fields of application is their short time-to-result and high sensitivity. Microplate-based assays such as ELISA have been adopted early in environmental and food analysis. Yet, to make immunoassays even faster, more sensitive, robust, and, most desirable, portable, advanced materials, sometimes developed for other purposes, can be profitably used to achieve these goals. Materials can be novel labels, e.g., chemical or particle labels, such as fluorophores or nanoparticles. Carrier particles, such as magnetic or polymer beads, make it possible to adopt the assays to meso- or microfluidic set-ups and encoding them opens the path to multiplex analysis. Specialty electrodes can enable for higher sensitivity in electrochemical detection. All this broadens the scope of application and lowers effort and cost for analysis at the point-of-need. T2 - ChemForum - Kolloquium des Instituts für Strukturchemie CY - Lisbon, Portugal DA - 07.09.2022 KW - Immunoassay KW - Biosensor KW - Antibody PY - 2022 AN - OPUS4-56782 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Anthropogenic markers quantified by rapid immunochemical methods – what can their occurrence in wastewater, surface water, and drinking water tell us? N2 - In urban waters, a multitude of organic micropollutants, often termed emerging pollutants, has been found over the last decades. Analytical methods suitable for trace analysis are needed that are desirably also fast, inexpensive and, if possible, robust and portable. Immunoanalytical, i.e., antibody-based, methods which are available in a broad range of formats, can be profitably used here to screen for the distribution and to monitor the trends of concentration levels of contaminants of emerging concern in the environment. Some of these formats are single-analyte but high-throughput methods. To use them wisely, indicator substances, sometimes called anthropogenic markers, should be selected and used in screening approaches, i.e., as indicators for contamination and the pre-selection of samples at which to have a closer look by multiplex methods like LC-MS/MS. Other methods are suitable to be performed on portable instrumentation in the field (on-site) or in facilities such as wastewater treatment plants for on-line monitoring of the treatment and elimination process. Furthermore, array technologies have been established that allow for parallel (multiplex) analysis of several analytes of interest. The microtiter-plate based ELISA (Enzyme-linked Immunosorbent Assay) is the method of choice for the analysis of a large number of samples [1]. ELISAs are available to monitor for anthropogenic markers such as the antiepileptic carbamazepine, the analgesic diclofenac, the antihistaminic cetirizine, the steroid hormone estrone, the antimicrobial sulfamethoxazole, psychoactive caffeine and cocaine, the priority pollutant bisphenol A, and the bile acid isolithocholic acid. For on-site screening and monitoring, simpler formats, like mix-and-read assays, e.g., the Fluorescence Polarization Immunoassay (FPIA) [2] or Lateral-flow Immunoassays (LFIA) [3] are more suitable tools, the latter based on dipsticks or little cassettes, with which users have become very familiar during the COVID-19 pandemic via rapid antigen tests. The suitability of multi-analyte formats such as immunomicroarrays depends on the choice of a signal-producing system that provides small uncertainties and good reproducibility of the measurements. Bead-based (“suspension”) arrays, read out in flow cytometers, are a powerful platform for multiplex assays [4]. Electrochemical formats, run on portable devices, provide additional advantages as no light source is required. They are most promising for stand-alone analysers and biosensors [5]. The speed, low cost and on-site capabilities of these methods allow to gather a lot more data on anthropogenic compounds which enables to quantify inputs, differences in degradation power of elimination processes, dilution phenomena and a more precise image of individual water cycles which is demonstrated by several examples. T2 - EuChemS Chemistry Congress ECC8 CY - Lisbon, Portugal DA - 28.08.2022 KW - Biosensor KW - Immunoassay KW - Antibody PY - 2022 AN - OPUS4-56783 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Environmental Analysis at BAM N2 - The talk is to introduce BAM, the Department of Analytical Chemistry; Reference Materials and the Division of Environmental Analysis and its works to the audience. T2 - Seminar des Centre for Environmental and Marine Studies (CESAM) der Universität Aveiro CY - Aveiro, Portugal DA - 12.09.2022 KW - Immunoassay KW - Biosensor KW - Antibody PY - 2022 AN - OPUS4-56784 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Field Analysis by Antibody-based Analytical Methods: The Importance of Advanced Materials N2 - Analytical methods based on the selectivity of antibodies, often called immunoassays, are a back-bone of clinical laboratory diagnostics. To bring them to the field, i.e., to make immunoanalytical methods portable, hopefully even faster, more sensitive, and robust, advanced materials are re-quired. Materials can be novel labels, e.g., chemical or particle labels, such as fluorophores or na-noparticles. Carrier particles, such as magnetic or polymer beads, make it possible to adopt the as-says to meso- or microfluidic set-ups and encoding them opens the path to multiplex analysis. Spe-cialty electrodes can enable for higher sensitivity in electrochemical detection. Without research into better materials, efforts to bring analysis to the point-of-need will not bear fruit. T2 - Kolloquium des Aveiro Institut of Materials (CICECO) der Universität Aveiro CY - Aveiro, Portugal DA - 20.09.2022 KW - Immunoassay KW - Biosensor KW - Antibody PY - 2022 AN - OPUS4-56785 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Antikörperbasierte Sensorik für das Monitoring von Spurenstoffen im Wasser N2 - Der Vortrag beschreibt den Stand der Arbeiten an der BAM, Fachbereich Umweltanalytik zur Entwicklung von Sensoren für die Wasseranalytik. Er stellt die bisherigen Arbeiten da, die ausgehend von ELISAs, zunehmend portable Plattformen wie FPIA und LFIA umfassen. Beas-based Formate werden von mikrotiterplatten-basierten Techniken auf mikrofluidische Lab-on-Chip-Systeme übertragen, die bei geeigneter Miniaturisierung und Automatisierung als Sensorsysteme angesehen werden können. Der Vortrag endet mit einem Blick auf ein laufendes Monitoring-Projekt von anthropogenen Markern in Abwasser und charakterisiert die Treiber, die die Entwicklung von Sensoren für die Wasseranalytik in den letzten Jahren vorantreiben. T2 - Sitzung des Fachausschusses Wasser der Wasserchemischen Gesellschaft in der GDCh CY - Online meeting DA - 16.01.2024 KW - Biosensoren KW - Immunoassay KW - Antikörper PY - 2024 AN - OPUS4-59383 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Riedel, Soraya A1 - Jaut, Valerie A1 - Schneider, Rudolf T1 - Ergometrine sensing in rye flour by a magnetic bead-based immunoassay followed by flow injection analysis with amperometric detection N2 - A certain group of mycotoxins, the ergot alkaloids, has caused countless deaths throughout human history. They are found in rye and other cereals and ingesting contaminated foods can cause serious health problems. To identify contaminated food exceeding the legal limits for ergot alkaloids, a portable and cost-effective test system is of great interest to the food industry. Rapid analysis can be achieved by screening for a marker compound, for which we chose ergometrine. We developed a magnetic bead-based immunoassay for ergometrine with amperometric detection in a flow injection system using a handheld potentiostat and a smartphone. With this assay a limit of detection of 3 nM (1 μg/L) was achieved. In spiked rye flour, ergometrine levels from 25 to 250 μg/kg could be quantified. All results could be verified by optical detection. The developed assay offers great promise to meet the demand for on-site ergometrine detection in the food industry. KW - Ergot alkaloids KW - Amperometry KW - Magnetic beads KW - Immunoassay KW - Food analysis KW - Fow injection analysis KW - Mycotoxins PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-565906 DO - https://doi.org/10.1016/j.talanta.2022.124172 SN - 0039-9140 N1 - Geburtsname von Riedel, Soraya: Höfs, S. - Birth name of Riedel, Soraya: Höfs, S. VL - 254 IS - 124172 SP - 1 EP - 8 PB - Elsevier B.V. AN - OPUS4-56590 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - Vortrag zum Kick-off des Projektes. Ziel des Projektes ist es, die Eignung der Bestimmung eines oder mehrerer der genannten anthropogenen Marker im Abwasser zur Volumenkorrektur („Kalibration“) im SARS-CoV-2-Monitoring von Abwässern auf der Basis eines von der BAM zu erhebenden Messdatensatzes zu evaluieren. T2 - Kick-off Meeting zum Projekt MARKERIA (VH1802) CY - Berlin, Germanyy DA - 01.04.2022 KW - Biosensoren KW - Immunoassay KW - SARS-CoV-2 PY - 2022 AN - OPUS4-57109 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - ESI-CorA ist ein nationales Verbundprojekt mit dem Ziel des Nachweises von SARS-CoV-2 im Abwasser. Das UBA hat als Teilprojekt das Projekt MARKERIA (VH1802) an die BAM vergeben. Ziel des Projektes ist es, die Eignung der Bestimmung eines oder mehrerer der genannten anthropogenen Marker im Abwasser zur Volumenkorrektur („Kalibration“) im SARS-CoV-2-Monitoring von Abwässern auf der Basis eines von der BAM zu erhebenden Messdatensatzes zu evaluieren. T2 - 12. Sitzung des Begleitkreises des nationalen Verbundprojektes ESI-CorA CY - Online meeting DA - 13.06.2022 KW - Biosensoren KW - Immunoassay KW - SARS-CoV-2 PY - 2022 AN - OPUS4-57110 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - 1. Projektreffen mit dem Umweltbundesamt für das Projekt MARKERIA (VH1802). Ziel des Projektes ist es, die Eignung der Bestimmung eines oder mehrerer der genannten anthropogenen Marker im Abwasser zur Volumenkorrektur („Kalibration“) im SARS-CoV-2-Monitoring von Abwässern auf der Basis eines von der BAM zu erhebenden Messdatensatzes zu evaluieren. T2 - 1. Projekttreffen mit dem UBA zum Projekt MARERIA (VH1802) CY - Berlin, Germany DA - 15.09.2022 KW - Biosensoren KW - Immunoassay KW - SARS-CoV-2 PY - 2022 AN - OPUS4-57111 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - 2. Projekttreffen mit dem Umweltbundesamt zum Projekt MARKERIA (VH1802). Ziel des Projektes ist es, die Eignung der Bestimmung eines oder mehrerer der genannten anthropogenen Marker im Abwasser zur Volumenkorrektur („Kalibration“) im SARS-CoV-2-Monitoring von Abwässern auf der Basis eines von der BAM zu erhebenden Messdatensatzes zu evaluieren. T2 - 2. Projekttreffen mit dem UBA zum Projekt MARKERIA (VH1802) CY - Berlin, Germany DA - 25.11.2022 KW - Biosensoren KW - Immunoassay KW - SARS-CoV-2 PY - 2022 AN - OPUS4-57112 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Ali, Syrine T1 - Antibiotics in wastewater: Optimisation of an ELISA Assay for the Detection of Clarithromycin in Wastewater N2 - Since they were first discovered, antibiotics have been used extensively to treat diseases caused by bacteria. This increasing and uncontrollable use is causing a number of environmental problems. Unfortunately, traces of various antibiotics can be found in drinking water also in wastewater. In this study was focused on the detection of clarithromycin (CLA) in wastewater using the immunofluorescence ELISA assay which is a highly selective and sensitive technique. ELISA is a multi-type of technique and the one used in this study was the indirect competitive ELISA. For the detection of CLA, a checkerboard was performed to obtain a dilution factor until 1:1,000,000 for the antigen (CLA-BSA) with a concentration of 5 ng/mL and a dilution factor until 1:5,120,00 for the primary antibody (anti-CLA mAb) with a concentration of 1. 035 ng/mL. Standardization was performed to obtain a better profile of the 4-parameter curve A, B, C and D which determines the maximum, minimum and inflection concentration. In addition, a pure synthetic water experiment was used to determine the stability of the assay and the matrix effect due to some interactions from chemicals or components used during the assays. The detection of CLA in pure water in Germany showed that the concentration of CLA differs from province to province and differs from time of year, however, the commonly detected concentration of CLA in pure water was ng/L. KW - Immunoassay KW - Clarithromycin KW - Abwasser KW - Anthropogener Marker KW - Antibiotikum PY - 2024 SP - 1 EP - 51 PB - Berliner Hochschule für Technik CY - Berlin AN - OPUS4-61027 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schmidt, Stephan A1 - Hoffmann, Holger A1 - Garbe, Leif-Alexander A1 - Harrer, Andrea A1 - Steiner, Markus A1 - Himly, Martin A1 - Schneider, Rudolf T1 - Re-assessment of monoclonal antibodies against diclofenac for their application in the analysis of environmental waters N2 - The non-steroidal anti-inflammatory drug (NSAID) diclofenac (DCF) is an important environmental contaminant occurring in surface waters all over the world, because, after excretion, it is not adequately removed from wastewater in sewage treatment plants. To be able to monitor this pollutant, highly efficient analytical methods are needed, including immunoassays. In a medical research project, monoclonal antibodies against diclofenac and its metabolites had been produced. Based on this monoclonal anti-DCF antibody, a new indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed and applied for environmental samples. The introduction of a spacer between diclofenac and the carrier protein in the coating conjugate led to higher sensitivity. With a test midpoint of 3 mg L−1 and a measurement range of 1–30 mg L−1, the system is not sensitive enough for direct analysis of surface water. However, this assay is quite robust against matrix influences and can be used for wastewater. Without adjustment of the calibration, organic solvents up to 5%, natural organic matter (NOM) up to 10 mg L−1, humic acids up to 2.5 mg L−1, and salt concentrations up to 6 g L−1 NaCl and 75 mg L−1 CaCl2 are tolerated. The antibody is also stable in a pH range from 3 to 12. Cross-reactivity (CR) of 1% or less was determined for the metabolites 40-hydroxydiclofenac (40-OH-DCF), 5-hydroxydiclofenac (5-OH-DCF), DCF lactam, and other NSAIDs. Relevant cross-reactivity occurred only with an amide derivative of DCF, 6-aminohexanoic acid (DCF-Ahx), aceclofenac (ACF) and DCF methyl ester (DCF-Me) with 150%, 61% and 44%, respectively. These substances, however, have not been found in samples. Only DCF-acyl glucuronide with a cross-reactivity of 57% is of some relevance. For the first time, photodegradation products were tested for cross-reactivity. With the ELISA based on this antibody, water samples were analysed. In sewage treatment plant effluents, concentrations in the range of 1.9–5.2 mg L−1 were determined directly, with recoveries compared to HPLC-MS/MS averaging 136%. Concentrations in lakes ranged from 3 to 4.4 ng L−1 and were, after pre-concentration, determined with an average recovery of 100% KW - Antikörper KW - Immunoassay KW - Wasser PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-602280 DO - https://doi.org/10.1039/d3ay01333b VL - 16 IS - 21 SP - 3349 EP - 3363 PB - Royal Society of Chemistry (RSC) CY - London AN - OPUS4-60228 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ecke, Alexander A1 - Bell, Jérémy A1 - Schneider, Rudolf T1 - A three-dimensional microfluidic flow cell and system integration for improved electrochemical substrate detection in HRP/TMB-based immunoassays N2 - Immunoassays, based on the recognition and capture of analytes by highly selective antibodies, are now used extensively in all areas of diagnostics, but the challenge is to further integrate them into online sensors. To improve the transition from laboratory immunoassays to immunosensors, we have developed a complete flow system, based on a microfluidic core flow cell to enable automated detection of one of the most commonly used immunoassay substrates, TMB, by chronoamperometry. The architecture and fluidic optimisation of the system showed that a specially designed 3D flow cell allows higher flow rates (500 μL min−1) than a standard enlarged microfluidic channel (50 μL min−1) resulting in a significantly shorter detection time of 30 seconds per sample and making the system more robust against interferences due to bubble formation in the chip. The electrochemical measurements showed an improved signal-to-noise ratio (SNR) and thus higher sensitivity for a model immunoassay for diclofenac (SNR = 59), compared to the analytical performance of a conventional laboratory microplate-based assay with optical detection (SNR = 19). In general, this system facilitates the conversion of any conventional immunoassay into an immunosensor with automatic and continuous detection. KW - Microfluidic KW - Immunoassay KW - Electrochemical KW - Mikrofluidik KW - Immunassay KW - Elektrochemie PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-580159 DO - https://doi.org/10.1039/d3sd00095h VL - 2 SP - 887 EP - 892 PB - Royal Society of Chemistry CY - London, United Kingdom AN - OPUS4-58015 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Konthur, Zoltán T1 - Tracking Human-Derived Contaminants in Wastewater: ELISA-Based Detection of Anthropogenic Markers in Samples from German Treatment Plants N2 - Wastewater-based epidemiology (WBE) plays a key role in pandemic management as an early warning system and a tool for guiding interventions. In Germany, combined sewer systems are prevalent, leading to fluctuations in wastewater volume and composition due to rainwater inflow, especially through street drains. This variability complicates the quantitative measurement of pathogens and pollutants in wastewater. While human excretion provides constant inputs, these parameters are often non-specific, lost, or degraded during transport. In contrast, pharmaceuticals excreted by humans, such as carbamazepine and diclofenac, as well as consumed caffeine, are fairly stable and serve as "anthropogenic markers." The simultaneous detection of these markers aids in normalizing WBE studies. Antibody-based methods, particularly Enzyme-Linked Immunosorbent Assays (ELISA), offer a cost-effective alternative to complex chromatographic techniques for monitoring anthropogenic markers. They demonstrate superior normalization quality compared to the frequently used quantitative PCR-based marker Pepper mild mottle virus (PMMoV). T2 - ANAKON 2025 CY - Leipzig, Germany DA - 10.03.2025 KW - Immunoassay KW - Spurenstoffe KW - Carbamazepin KW - anthropogener Marker KW - Koffein PY - 2025 AN - OPUS4-63494 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Konthur, Zoltán T1 - Bundesweite Bestimmung anthropogener Marker im Rohabwasser mittels Immunoassays N2 - Die von der Europäischen Kommission im Jahr 2021 empfohlene, systematische Abwasserüberwachung mittels PCR-Techniken wird als ein wirksames Frühwarnsystem für künftige Pandemien durch Viren und andere Krankheitserreger betrachtet. Ein generelles Problem bei jeder quantitativen Messung von Parametern im Abwasser ist die starke Schwankung des Abwasservolumens und seiner Zusammensetzung im Tages- und Jahreszeitenverlauf. Um die Konzentrationsbestimmungen von Krankheitserregern im Abwasser in Korrelation mit menschlichen Einträgen setzen zu können, könnten sog. "anthropogene Marker" eine herausragende Rolle spielen, deren Eintrag ins Abwasser menschlichen Ursprungs sind. Im Projekt werden mittels ELISA die Konzentrationen ausgewählter anthropogener Marker in deutschlandweit gesammelten Abwasserproben erhoben und analysiert. Die Messergebnisse zeigen eindeutige Standortunterschiede auch in Abhängigkeit der Kläranlagengröße und vorhandener Indirekteinleiter. Die Untersuchungen zeigen, dass nicht alle anthropogene Marker an allen Standorten für eine Normalisierung von PCR-Daten für epidemiologische Untersuchungen verwendbar sind. T2 - Wasser 2024 (Jahrestagung der Wasserchemischen Gesellschaft der GDCh) CY - Limburg an der Lahn, Germany DA - 06.05.2024 KW - Diclofenac KW - Koffein KW - Carbamazepin KW - Isolithocholsäure KW - Immunoassay PY - 2024 AN - OPUS4-60991 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Exner, T. T1 - Evaluation anthropogener Marker als Normalisierungsfaktoren für die SARS-CoV-2 Abwassersurveillance zur epidemiologischen Lagebewertung N2 - Im Rahmen der weltweiten Corona-Pandemie fordert die EU-Kommission seit März 2021 die systematische Erfassung und Überwachung von SARS-CoV-2 über das Abwasser, welche der Entscheidungsfindung bezüglich bevölkerungsbezogener Maßnahmen dienen soll. Hierbei können Trendanalysen für die epidemiologische Lagebewertung abgeleitet werden, sofern ein großer Anteil des kommunalen Abwassers der Bevölkerung durch das Monitoring abdeckt wird. Die Datenerhebung erfolgt somit unabhängig vom individuellen Test- und Meldeverhalten der Bevölkerung. Eine bedeutende Herausforderung bei der Bestimmung der Gensequenzen mittels polymerase chain reaction (PCR) stellen standortabhängige Faktoren des Einzugsgebietes sowie Schwankungen der Durchflussrate und der Abwasserzusammensetzung dar. Zu den möglichen Ursachen zählen Verdünnungseffekte durch Regen- und Schmelzwasser, Größenunterschiede des kommunalen Entwässerungssystems, Veränderungen der Demografie und Zuflüsse von Indirekteinleitern. Um eine verbesserte Korrelation quantitativ ermittelter Genkopien des SARS-CoV-2 aus der jeweiligen Abwasserprobe mit dem Anteil der infizierten Bevölkerung zu erzeugen, ist eine Normalisierung der PCR Daten vorzunehmen. Neben relativ einfach zu ermittelnden physikalischen Größen, wie der mittleren Durchflussrate oder der Leitfähigkeit, die Aufschlüsse über den Anteil von Fremd- und Regenwasser ermöglichen, können auch anthropogene Marker, wie Spurenstoffe und Surrogatviren im Abwasser, als direkte Indikatoren für menschliche Fäkalien aus dem häuslichen Abwasser dienen. Im Folgenden soll eine vergleichende Untersuchung von vier Spurenstoffen ‒ Koffein (CAF), Carbamazepin (CBZ), Diclofenac (DCF), Isolithocholsäure (ILA) ‒ sowie einem Surrogatvirus (SV) (Pepper mild mottle virus (PMMoV) oder CrAssphage), die Eignung der jeweiligen Paramater als Normalisierungsfaktoren analysieren. Die Untersuchung fand an 19 Standorten über drei Monate im Rahmen einer Studie aus dem Projekt ESI-CorA statt. T2 - Wasser 2024 (Jahrestagung der Wasserchemischen Gesellschaft der GDCh) CY - Limburg an der Lahn, Germany DA - 06.05.2024 KW - ELISA KW - Immunoassay PY - 2024 AN - OPUS4-60992 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Spurenstoffe im Rohabwasser: Möglichkeiten und Perspektiven für den One-Health-Ansatz N2 - Der One-Health-Ansatz hat die Gesundheit von Mensch, Tier und Umwelt im Fokus. Dazu ist eine interdisziplinäre Zusammenarbeit von Akteuren aus Human- und Veterinärmedizin sowie den Umweltwissenschaften notwendig. Ein Ineinandergreifen von methodischen Ansätzen und der Analytik wird angestrebt. Im Zuge der anstehenden Novellierung (2924) der EU-Kommunalabwasserrichtlinie (91/271/EWG) wird die Umsetzung eines One-Health-Ansatzes bereits adressiert. Dazu gehört zum einen der Ausbau und die Prozessüberwachung einer vierten Reinigungsstufe in Klärwerken. Zum anderen ist europaweit die Infrastruktur einer Abwassersurveillance zur Überwachung von Krankheitserregern zu gewährleisten. Dies wird aktuell in Deutschland mit dem Projekt AMELAG ("Abwassermonitoring zur epidemiologischen Lagebewertung") mit zwei Probennahmen pro Woche auf ca. 170 Kläranlagen umgesetzt. T2 - 6. Mülheimer Wasseranalytisches Seminar (MWAS 2024) CY - Mülheim an der Ruhr, Germany DA - 10.09.2024 KW - ELISA KW - Spurenstoffe KW - Koffein KW - Carbamazepin KW - Diclofenac KW - Immunoassay PY - 2024 AN - OPUS4-60993 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Ecke, Alexander A1 - Bell, Jérémy A1 - Schneider, Rudolf T1 - From Laboratory Immunoassays to Immunosensors with an Integrated Microfluidic Flow System N2 - This research summary describe efforts to the development of a three-dimensional microfluidic flow cell for improved electrochemical substrate detection in HRP/TMB-based immunoassays. This work provides a valuable advancement in the sensitivity and integration of immunoassay technologies, aiding in the refinement of diagnostic tools. KW - Antikörper KW - Immunoassay KW - Microfluidics KW - Electrochemical detection KW - Biosensor PY - 2025 UR - https://www.elveflow.com/microfluidics-research-summaries/from-laboratory-immunoassays-to-immunosensors-with-an-integrated-microfluidic-flow-system/ SP - 1 EP - 4 CY - Paris AN - OPUS4-62674 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Monitoring trace substances in wastewater and surface water by immunoanalytical assays and sensors N2 - Monitoring the elimination of trace substances in wastewater treatment plants, as required in the recast of the EU Urban Wastewater Treatment Directive and the new Kommunalabwasserrichtlinie (KARL), creates a need for on-site analyses in wastewater treatment plants. Immunoanalytical methods can be carried out on site, e.g. in the form of rapid tests, but continuously operating (immuno-bio)sensors would be ideal. And antibody-based methods have the inherent disadvantage that one antibody must be used per analyte and therefore only a small number of substances can be analysed. A set of indicators has been integrated in the directives in accordance with the list of the Kompetenzzentrum Spurenstoffe BW (KomS) and offer points of reference here. Yet, in order to be able to use immunoanalytical methods for their monitoring, (DIN/ISO) standards are required that define the necessary quality requirements and quality management measures for the sensors. In addition, for the larger part of the indicators, the required antibodies have yet to be developed and made commercially available in the long term. The talk will highlight our previous work on high-throughput methods, such as ELISA, regarding the presence of anthropogenic markers and some indicators as well as research approaches for sensor technology that could be used on-site, and will provide an overview of deficits and future efforts. T2 - Kolloquium der Abteilung Technische Umweltchemie und Sensortechnik CY - Stuttgart, Germany DA - 21.05.2025 KW - Antibodies KW - Immunoassay KW - Microfluidics KW - Biosensor KW - Trace substances PY - 2025 AN - OPUS4-63501 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Schneider, Rudolf T1 - Wasserdiagnose durch die Verwendung von Markern N2 - Die Überwachung des gesamten Wasserkreislaufs auf Mikroverunreinigungen ist aus gesundheitlicher und ökologischer Hinsicht wünschenswert und gewinnt durch den Klimawandel und das zunehmende Erfordernis der Wiederverwendung des Brauchwassers weiter an Bedeutung. Die schnellen und kostengünstigen antikörperbasierten Methoden („Immunoassays“) können helfen, Informationslücken zu schließen und kürzere Reaktionszeiten zu ermöglichen. Von Labormethoden über tragbare Analysengeräte bis hin zu Online-Sensoren sind viele Formate möglich (u. a. ELISA, Abb. 1). Es muss zudem die Bereitstellung der notwendigen, hochqualitativen Antikörper gewährleistet werden. Da aber gilt „1 Analyt – 1 Antikörper“, ist die Herstellung eines Konsenses bezüglich der vordringlich zu bestimmenden Indikatoren/Marker notwendig. Auch die Normung der Verfahren ist parallel in Angriff zu nehmen. KW - Antikörper KW - Immunoassay KW - Abwasser KW - ELISA PY - 2025 UR - https://analyticalscience.wiley.com/content/article-do/wasserdiagnose-durch-die-verwendung-von-markern VL - 2025 IS - 1 SP - 1 EP - 4 PB - WILEY CY - Hoboken, NJ, USA AN - OPUS4-63508 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Geißler, Daniel A1 - Wegner, Karl David A1 - Fischer, C. A1 - Resch-Genger, Ute T1 - Exploring Simple Particle-Based Signal Amplification Strategies in a Heterogeneous Sandwich Immunoassay with Optical Detection N2 - Heterogeneous sandwich immunoassays are widely used for biomarker detection in bioanalysis and medical diagnostics. The high analyte sensitivity of the current “gold standard” enzyme-linked immunosorbent assay (ELISA) originates from the signal-generating enzymatic amplification step, yielding a high number of optically detectable reporter molecules. For future point-of-care testing (POCT) and point-of-need applications, there is an increasing interest in more simple detection strategies that circumvent time-consuming and temperature-dependent enzymatic reactions. A common concept to aim for detection limits comparable to those of enzymatic amplification reactions is the usage of polymer nanoparticles (NP) stained with a large number of chromophores. We explored different simple NP-based signal amplification strategies for heterogeneous sandwich immunoassays that rely on an extraction-triggered release step of different types of optically detectable reporters. Therefore, streptavidinfunctionalized polystyrene particles (PSP) are utilized as carriers for (i) the fluorescent dye coumarin 153 (C153) and (ii) hemin (hem) molecules catalyzing the luminol reaction enabling chemiluminescence (CL) detection. Additionally, (iii) NP labeling with hemin-based microperoxidase MP11 was assessed. For each amplification approach, the PSP was first systematically optimized regarding size, loading concentration, and surface chemistry. Then, for an immunoassay for the inflammation marker C- eactive protein (CRP), the analyte sensitivity achievable with optimized PSP ystems was compared with the established ELISA concept for photometric and CL detection. Careful optimization led to a limit of detection (LOD) of 0.1 ng/mL for MP11-labeled PSP and CL detection, performing similarly well to a photometric ELISA (0.13 ng/mL), which demonstrates the huge potential of our novel assay concept. KW - Nanoparticle KW - Fluorescence KW - Immunoassay KW - Quality assurance KW - Antibody KW - Polymer KW - Dye KW - Signal enhancement KW - CRP KW - Biosensing PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-597853 DO - https://doi.org/10.1021/acs.analchem.3c03691 SN - 1520-6882 VL - 96 IS - 13 SP - 5078 EP - 5085 PB - American Chemical Society CY - Columbus, Ohio AN - OPUS4-59785 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - López-Puertollano, Daniel A1 - Tobias, Charlie A1 - Bell, Jérémy A1 - Abad-Somovilla, A. A1 - Abad-Fuentes, A. A1 - Rurack, Knut T1 - Superparamagnetic Bead-Based Microfluidic Fluoroimmunoassay Platform for Rapid Ochratoxin A Detection in Flour N2 - Simplification and reduction of time and costs are the primary goals in the development and use of onsite methods in diagnostics and food safety. To facilitate the transition from laboratory techniques to simple, miniaturized devices, we have developed a modular microfluidic platform. This platform integrates a competitive fluorescence immunoassay on the surface of superparamagnetic beads, serving as a complementary technique to traditional cytometry assays. In the first chip module, a fast competitive reaction (5 min) occurs, after which the particles are retained in the second module. This module consists of a PDMS chip and a permanent magnet, allowing only the fluorescent competitor to reach the detection module. Ochratoxin A (OTA) was chosen as the model analyte for device development, using fluorescein-labeled OTA as a competitor. The system efficiently separates particles, with OTA concentration directly correlated to the amount of fluorescent competitor remaining in solution after the competitive reaction. This innovative setup allows to perform rapid measurements with small sample volumes in a short time (10 min), achieving a limit of detection for OTA of 1.2 μg L–1. The system was successfully applied to the accurate determination of OTA in wheat flour spiked at regulatorily relevant concentrations. Using this device, conventional cytometry immunoassays can be seamlessly transformed into user-friendly, miniaturized analytical methods at reduced cost for applications outside of a laboratory directly at the point of need. KW - Bead-based assay KW - Fluorescence KW - Immunoassay KW - Microfluidics KW - mycotoxins PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-638472 DO - https://doi.org/10.1021/acssensors.5c01119 SN - 2379-3694 SP - 1 EP - 10 PB - American Chemical Society CY - Washington, D.C. AN - OPUS4-63847 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Exner, Thomas T1 - Spurenstoffe im Rohabwasser als Normalisierungsparameter N2 - Im Zuge der aktualisierten Kommunalabwasserrichtlinie (EU 2024/3019) bekommen u.a. Spurenstoffe und Krankheitsüberwachung eine erhöhte Aufmerksamkeit. Zum einen sollen ausgewählte Spurenstoffe zur zukünftigen Überwachung der Funktionalität der vierten Reinigungsstufe eingesetzt werden. Zum anderen ist europaweit die Infrastruktur einer Abwassersurveillance für mehrere Krankheitserreger zu gewährleisten. Letzteres wird seit 2022 in Deutschland mit den Projekten ESI-CORA und AMELAG mit wöchentlich je zwei Probennahmen auf 20 bis 170 Kläranlagen umgesetzt. In der vorliegenden Arbeit wurde geprüft, ob das Monitoring von Spurenstoffen zum Ausgleich von starken Schwankungen in der Viruslast verwendet werden kann (Normalisierung). T2 - 16. Langenauer Wasserforum (LWF) CY - Langenau, Germany DA - 17.11.2025 KW - Carbamazepin KW - Immunoassay KW - Anthropogene Marker KW - ELISA KW - Spurenstoffe PY - 2025 AN - OPUS4-64809 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Vergin, Chantal C. T1 - Entwicklung von Antikörpern gegen Mykotoxine und ihre Anwendung in immunchemischen Verfahren N2 - Die Gewährleistung der Lebensmittelsicherheit stellt ein zentrales und aktuelles Thema in unserer Gesellschaft dar und ist im Lebensmittelrecht gesetzlich verankert. Pilzgifte, sogenannte Mykotoxine, sind sekundäre Stoffwechselprodukte, die von Schimmelpilzen gebildet werden. Durch den Befall von Lebensmitteln können diese Kontaminanten in die Nahrung und somit in die Nahrungskette gelangen, wodurch sie ein ernstes Gesundheitsrisiko für Mensch und Tier darstellen. Aus diesem Grund hat die Europäische Kommission unter anderem die Verordnung (EU) 2023/915 erlassen, welche Höchstwerte für bestimmte Mykotoxine in Lebensmitteln festlegt. Derzeit sind etwa 25 % der Lebensmittel mit Mykotoxinen oberhalb der gesetzlich vorgeschriebenen Grenzwerte belastet. Um derartige Überschreitungen zu vermeiden, sind regelmäßige Kontrollen unerlässlich. Die derzeit verfügbaren analytischen Verfahren, die überwiegend auf chromatographischen Methoden wie LC-MS/MS basieren, werden von der Lebensmittelindustrie als unzureichend angesehen, weil sie technisch aufwendig, arbeitsintensiv und nicht vor Ort – d.h. an Verarbeitungs- und Produktionsstandorten –einsetzbar sind. Eine Möglichkeit der Verbesserung ist der Einsatz von Immunoassays, deren Anwendung in der Medizin weit verbreitet sind. Grundvoraussetzung für die Entwicklung solcher Assays ist jedoch die Verfügbarkeit spezifischer Antikörper. Unser Schwerpunkt in diesem Projekt liegt auf der Entwicklung monoklonaler, hochaffiner und hochselektiver Antikörper gegen Mykotoxine, für die bislang keine oder nur polyklonale beziehungsweise in ihrer Spezifität unzureichende Antikörper existieren. Ziel ist die Herstellung von Antikörpern gegen Patulin, Alternaria-Toxine sowie Mutterkornalkaloide, um diese in Schnelltests und vor-Ort-Analytiksystemen einsetzen zu können. Ein Fokus liegt auf der Gruppenselektivität der Antikörper im Hinblick auf die verschiedenen Mutterkornalkaloide. Für die Immunisierung der Mäuse werden – je nach Mykotoxin – heterologe oder homologe Haptene verwendet. Zur Sicherstellung der Monoklonalität kommen Methoden wie die Begrenzungsverdünnung sowie die antigenspezifische fluoreszenzaktivierte Zellsortierung zum Einsatz. Die im Projekt gewonnenen Antikörper sollen zur Erweiterung des bestehenden Mykotoxin-Testkits der SAFIA Technologies GmbH eingesetzt werden, um eine zuverlässige Detektion von Mykotoxinen in Lebensmitteln zu gewährleisten und so einen Beitrag zur Verbesserung der Lebensmittelsicherheit zu leisten. Es werden der verfolgte Ansatz und die bisher erreichten Ergebnisse vorgestellt. T2 - 53. Deutsche Lebensmittelchemietage CY - Halle (Saale), Germany DA - 22.09.2025 KW - Antikörper KW - Biosensorik KW - Mykotoxine KW - Immunoassay PY - 2025 AN - OPUS4-64458 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Vergin, Chantal C. T1 - Development of antibodies against mycotoxins and their application in immunochemical methods N2 - Food safety is a central and topical issue in our society and is governed by food law. Mycotoxins are secondary metabolic products formed by molds. These contaminants can enter food and thus the food chain through infestation, posing a serious health risk to humans and animals. For this reason, the European Commission has issued Regulation (EU) 2023/915, which sets maximum levels for certain mycotoxins in food. Currently, around 25 % of foodstuffs are contaminated with mycotoxins above the legally prescribed limits. Regular checks are essential to prevent such exceedances. The analytical methods currently available, mainly based on chromatographic techniques such as LC-MS/MS, are considered inadequate for on-site use – i.e., at processing and production facilities in the food industry – because they are technically complex and labor-intensive. One possible improvement is the use of immunoassays, which are widely accepted and employed in medical diagnostics. However, a basic prerequisite for developing such assays is the availability of specific antibodies. Our focus in this project is the development of high-affinity, highly selective monoclonal antibodies against mycotoxins for which either no antibodies, only polyclonal antibodies, or antibodies with insufficient specificity are currently available. The goal is to generate antibodies targeting patulin, Alternaria toxins, and ergot alkaloids for use in rapid tests and on-site analytical systems. A key aspect is the group selectivity of the antibodies with regard to the various ergot alkaloids. Depending on the mycotoxin, heterologous or homologous haptens are used to immunize mice. To ensure monoclonality, we apply limiting dilution and antigen-specific fluorescence-activated cell sorting (FACS) during the selection process. The antibodies developed will be used to expand SAFIA Technologies GmbH’s existing mycotoxin test-kit, enabling reliable detection of mycotoxins in food and thereby contributing to improved food safety standards. We will showcase our approach along with the results achieved so far T2 - 5th European Biosensor Symposium CY - Tarragona, Spain DA - 26.10.2025 KW - Antibodies KW - Biosensor KW - Immunoassay KW - Mycotoxins PY - 2025 AN - OPUS4-64459 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Immunochemical sensing of key pollution indicators in the water cycle N2 - Organic micropollutants - often termed emerging contaminants - are increasingly detected throughout the water cycle, from drinking water to wastewater. Monitoring their distribution requires trace analytical methods that are fast, cost-effective, matrix-tolerant, and ideally portable. Immunoanalytical techniques, using antibodies, nanobodies, or fragments, offer high selectivity and sensitivity, though typically in single-analyte or oligoplexing formats. To maximize their utility, environmentally relevant indicator compounds should be selected to reflect pollutant input, partial removal during treatment, and persistence in wastewater and surface water. ELISA remains a robust method for high-throughput analysis. We have assays available for the com-pounds carbamazepine, diclofenac, cetirizine, estrone, sulfamethoxazole, caffeine, cocaine, bisphenol A, and isolithocholic acid. Simpler formats like fluorescence polarization immunoassays (FPIA) and lateral flow immunoassays (LFIA)—familiar from COVID-19 rapid tests — enable on-site screening. Multiplexing is feasible with bead-based microarrays and electrochemical sensors on microfluidic platforms, which additionally offer portability and the latter even independence from optical systems. Despite these advances, the development of highly selective, high-affinity binders remains a critical bottleneck. Promising strategies to overcome this include computational hapten design, nanobody engineering, and in silico optimization of binder sequences—enhanced by artificial intelligence for targeted database mining. These approaches, coupled with scalable recombinant expression, are poised to expand the binder repertoire essential for any immunoanalytical platform. This growing repository will be key to advancing immunochemical diagnostics across the water cycle. T2 - 5th International Symposium on Nanobody for Immunological Analysis and Application CY - Guangzhou, China DA - 15.11.2025 KW - Antibodies KW - Immunoassay KW - Microfluidics KW - Biosensor KW - Trace substances KW - Electrochemical detection PY - 2025 AN - OPUS4-64923 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - How can antibodies help us in the risk assessment of pharmaceuticals in the environment? N2 - The widespread use of pharmaceuticals has led to their persistent presence in various environmental compartments, raising concerns about ecological and human health risks since they pose a significant threat to non-target organisms and human health, necessitating robust methods for environmental risk assessment.   Traditional analytical methods, such as liquid chromatography-mass spectrometry (LC-MS), offer high sensitivity and specificity but are often resource-intensive, require complex sample preparation, limiting their applicability for large-scale or real-time monitoring. In this context, antibody-based analytical techniques present a promising complementary approach for the detection and quantification of pharmaceutical residues in environmental matrices.   Antibodies, due to their high specificity and affinity, enable the development of immunoassays such as ELISA, lateral flow assays, fluorescence-based formats, and electrochemical sensors that are rapid, cost-effective, and adaptable to field conditions. These methods can be tailored to target a wide range of pharmaceutical compounds, including antibiotics and endocrine disruptors. Moreover, antibody-based assays facilitate high-throughput screening and can be integrated into portable platforms for on-site analysis, thereby enhancing temporal and spatial resolution in environmental monitoring.   This presentation explores the potential of antibodies in environmental risk assessment, focusing on their application in detecting trace levels of pharmaceuticals in water, soil, and biota. They are presented as a complementary and powerful tool for rapid, cost-effective screening in the lab and that also can be deployed for on-site analysis in the field.   The talk also discusses recent advances in antibody engineering, multiplexing capabilities [1], and the integration of immunoassays with sensor technologies [2].   Case studies illustrate how immunoanalytical data can help in finding potential contamination hotspots [3], hazard identification, pollution source tracking, and the generation of crucial data for predictive models on environmental fate and effects. Antibody-based methods can also help in early warning systems and the evaluation of mitigation strategies, contributing to a more comprehensive and proactive risk assessment framework, eventually to regulatory decision-making, ultimately aiding in the protection of ecosystems and public health. T2 - ICRAPHE - 6th International Conference on Risk Assessment of Pharmaceuticals in the Environment CY - Aveiro, Portugal DA - 20.10.2025 KW - Antibodies KW - Immunoassay KW - Microfluidics KW - Electrochemical detection KW - Biosensor PY - 2025 AN - OPUS4-64922 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Riedel, Soraya T1 - Application of 3,3’,5,5’-Tetramethylbenzidine (TMB) in Amperometric Immunoassays for Mycotoxin Detection N2 - Electrochemical methods make great promise to meet the demand for user-friendly on-site devices for monitoring important parameters. Food industry often runs own lab procedures, e.g., for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with miniaturized technologies. Enzyme-linked immunosorbent assays, with photometric detection of the horseradish peroxidase (HRP) substrate 3,3’,5,5’-tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straight-forward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry, it could be shown that TMB electrochemistry is strongly dependent on the pH and the electrode material. It was found that screen-printed gold electrodes and a very low pH value (pH 1) are well-suited to perform the electrochemical detection of TMB, due to the reversible character of the redox reaction under these conditions. Under these conditions, a good signal stability over several measuring cycles is achieved, providing the basis for analyzing multiple samples. In contrast to this, for carbon screen-printed electrodes, it was found that the signal response has changed after the electrochemical reaction with TMB at pH 1. At moderately acidic conditions (pH 4), neither with carbon nor with gold electrodes a reproducible electrochemical detection of TMB could be achieved. Based on these findings, we created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A (OTA) and ergometrine in food samples. A competitive assay is performed on magnetic beads using HRP and TMB/H2O2 to generate the signal. Enzymatically oxidized TMB is quantified after addition of H2SO4 by amperometry with screen-printed gold electrodes in a custom-made wall-jet flow cell. The results are in good correlation with the established photometric detection method, providing a solid basis for sensing of further analytes in HRP-based assays using the newly developed miniaturized smartphone-based, electrochemical, immunomagnetic assay. T2 - 73rd Annual Meeting of the ISE CY - Online meeting DA - 12.09.2022 KW - Cylic Voltammetry KW - Immunoassay KW - Mycotoxins KW - Amperometry KW - Electrochemistry PY - 2022 N1 - Geburtsname von Riedel, Soraya: Höfs, S. - Birth name of Riedel, Soraya: Höfs, S. AN - OPUS4-55793 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Riedel, Soraya A1 - Hülagü, Deniz A1 - Bennet, Francesca A1 - Carl, Peter A1 - Flemig, Sabine A1 - Schmid, Thomas A1 - Schenk, J. A. A1 - Hodoroaba, Vasile-Dan A1 - Schneider, Rudolf T1 - Electrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’- Tetramethylbenzidine in Amperometric Assays N2 - Electrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for Monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3’,5,5’-tetramethylbenzidine (TMB),form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detectionstep, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphonebased, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis forsensing of further analytes. KW - Ochratoxin A KW - Amperometry KW - Cyclic voltammetry KW - Electrochemistry KW - Immunoassay PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-530421 DO - https://doi.org/10.1002/celc.202100446 N1 - Geburtsname von Riedel, Soraya: Höfs, S. - Birth name of Riedel, Soraya: Höfs, S. VL - 8 IS - 13 SP - 2597 EP - 2606 AN - OPUS4-53042 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Riedel, Soraya T1 - Steps Forward in the Application of 3,3’,5,5’- Tetramethylbenzidine (TMB) in Amperometric Assays N2 - Electrochemical methods make great promise to meet the demand for user-friendly on-site devices for monitoring important parameters. Food industry often runs own lab procedures, e.g. for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with miniaturized technologies. Enzyme-linked immunosorbent assays, with photometric detection of the horseradish peroxidase (HRP) substrate, 3,3’,5,5’-tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straight-forward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. It was found that screen-printed gold electrodes and a highly acidic pH value (pH 1) are well-suited to perform the electrochemical detection of TMB, due to the reversible character of the redox reaction under these conditions. This set-up provides a good signal stability over several measuring cycles, providing the basis for analysing multiple samples. In contrast to this, for carbon screen-printed electrodes, it was found that the signal response has changed after the electrochemical reaction with TMB at pH 1. At a weakly acidic pH value (pH 4), neither with carbon nor with gold electrodes a reproducible electrochemical detection of TMB could be achieved [1]. Based on these findings we created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A and ergometrine in real samples. Therefore, a competitive assay was performed on magnetic beads using HRP and TMB/H2O2 to generate the signal. Enzymatically oxidized TMB was quantified after addition of H2SO4 by amperometry with screen-printed gold electrodes in a custom-made wall-jet flow cell. The results are in good correlation with the established photometric detection method, providing a solid basis for sensing of further analytes in HRP-based assays using the newly developed miniaturized smartphone-based, electrochemical, immunomagnetic assay. T2 - Electrochemistry 2022 CY - Berlin, Germany DA - 28.09.2022 KW - Cylic Voltammetry KW - Immunoassay KW - TMB KW - Amperometry PY - 2022 N1 - Geburtsname von Riedel, Soraya: Höfs, S. - Birth name of Riedel, Soraya: Höfs, S. AN - OPUS4-55876 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -