TY - CONF A1 - Thümmler, Bodhi T1 - The Iron Quest: Unraveling Distribution and Bioavailability in Biofortified Vegetables N2 - Insufficient absorption of nutritional iron is the cause of the most prevalent dietary micronutrient deficiency in the world. Dietary iron deficiency disproportionally affects women and is the most common origin of anaemia (iron deficiency anaemia) globally. This underlines the requirement of dietary iron for the human body, which ranges between 11–27 mg(Fe) per day for adults depending on sex, pregnancy, and nursing status. In Germany, consumers have easy access to ferrous or ferric supplements or iron-fortified foods. However, iron-biofortified food products are not yet available and only 18 % of German consumers are familiar with the label “biofortified food” [1]. Hence, the BMBF-funded research project EiBiG was launched, aiming at establishing plant cultivation methods that raise plant iron content through foliar iron fertilization and increase the iron bioavailability in vitamin C-rich vegetables (e.g., baby-leaf spinach and bell pepper). Iron bioavailability will be studied using combined in vitro digestion/Caco-2 cell culture models [2]. Furthermore, to investigate foliar iron absorption mechanisms, the spatial iron distribution in biofortified & non-biofortified baby-leaf spinach is investigated using Laser Induced Breakdown Spectroscopy (LIBS), μXRF, & LA-ICP-MS. T2 - 38th Annual GMS Meeting CY - Munich, Germany DA - 24.10.2024 KW - Bioavailability KW - Iron KW - Spinach KW - Biofortified Food PY - 2024 AN - OPUS4-61486 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braymer, Joseph T1 - Methanobacterium IM1 as a model organism to study MIC N2 - Microbiologically influenced corrosion (MIC), also known as biocorrosion, is a process where microbes corrode metal surfaces causing detrimental damage to manmade infrastructure. Several studies, mainly under anaerobic conditions, have identified sulfate-reducing bacteria and methanogens as key players in biocorrosion. However, an important issue is that most MIC-related microbes at the moment are non-culturable. In addition, improved technologies (i.e., omics, biochemical, and microscopy methods) are needed to study, detect, simulate, and modulate MIC-related cases. Therefore, the selection of strains that can cause MIC, are culturable, and are genetically accessible, are of high importance for establishing new methods to study MIC on the molecular level. For example, the archaeal strain Methanobacterium IM1 has caught great attention due to its involvement in biocorrosion processes. Genes encoding for a novel enzyme (MIC NiFe-hydrogenase) have been proposed in this methanogen to be directly related to the e T2 - International Biodeterioration and Biodegradation Symposium 19 CY - Berlin, Germany DA - 11.09.2024 KW - Biocorrosion KW - Methanogen KW - Iron KW - Hydrogenase KW - Mechanism PY - 2024 AN - OPUS4-61508 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -