TY - JOUR A1 - Haustein, T. A1 - Busweiler, Sabine A1 - Haustein, V. A1 - von Laar, C. A1 - Plarre, Rüdiger T1 - Laboratory breeding of Korynetes caeruleus (Coleoptera: Cleridae) for the biological of Anobium punctatum) (Coleoptera, Ptinidae) N2 - Larvae and adults of Korynetes caeruleus (de Geer 1775) (Coleoptera: Cleridae) were collected from old churches and reared in the laboratory on Anobium punctatum (de Geer 1774) (Coleoptera: Ptinidae). Breeding success of K. caeruleus was low, but basic parameters of this species’ developmental cycle were identifi ed. At 21°C and 75% relative humidity and a fourmonth cold period at 4°C, the development of K. caeruleus from egg to adult appearance lasted 2 years. The pupal stage may be reached and completed after one and a half years. Feeding on larvae of A. punctatum by larvae of K. caeruleus was observed and consisted of a combination of sucking haemolymph and consuming body parts. The sickle-like mandibles of larvae of K. caeruleus penetrate the cuticle of prey larvae; this is followed by pumping and sucking body movements. Adult beetles of A. punctatum were not attacked by K. caeruleus larvae. Feeding behaviour of adult K. caeruleus was not investigated. KW - Cultural heritage KW - Coleoptera KW - Korynetes caeruleus KW - Cleridae KW - Ptinidae KW - Anobium punctatum KW - Biological pest control KW - Life history data KW - Laboratory breeding KW - Wood protection PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-494999 DO - https://doi.org/10.14411/eje.2019.038 SN - 1802-8829 VL - 116 SP - 362 EP - 371 PB - České Budějovice AN - OPUS4-49499 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - An, Biwen Annie A1 - Kleinbub, Sherin A1 - Özcan Sandikcioglu, Özlem A1 - Koerdt, Andrea T1 - Iron to Gas: Versatile Multiport Flow-Column Revealed Extremely High Corrosion Potential by Methanogen-Induced Microbiologically Influenced Corrosion (Mi-MIC) N2 - Currently, sulfate-reducing bacteria (SRB) is regarded as the main culprit of microbiologically influenced corrosion (MIC), mainly due to the low reported corrosion rates of other microorganisms. For example, the highest reported corrosion rate for methanogens is 0.065 mm/yr. However, by investigating methanogen-induced microbiologically influenced corrosion (Mi-MIC) using an in-house developed versatile multiport flow test column, extremely high corrosion rates were observed. We analyzed a large set of carbon steel beads, which were sectionally embedded into the test columns as substrates for iron-utilizing methanogen Methanobacterium IM1. After 14 days of operation using glass beads as fillers for section separation, the highest average corrosion rate of Methanobacterium IM1 was 0.2 mm/yr, which doubled that of Desulfovibrio ferrophilus IS5 and Desulfovibrio alaskensis 16109 investigated at the same conditions. At the most corroded region, nearly 80% of the beads lost 1% of their initial weight (fast-corrosion), resulting in an average corrosion rate of 0.2 mm/yr for Methanobacterium IM1-treated columns. When sand was used as filler material to mimic sediment conditions, average corrosion rates for Methanobacterium IM1 increased to 0.3 mm/yr (maximum 0.52 mm/yr) with over 83% of the beads having corrosion rates above 0.3 mm/yr. Scanning electron images of metal coupons extracted from the column showed methanogenic cells were clustered close to the metal surface. Methanobacterium IM1 is a hydrogenotrophic methanogen with higher affinity to metal than H2. Unlike SRB, Methanobacterium IM1 is not restricted to the availability of sulfate concentration in the environment. Thus, the use of the multiport flow column provided a new insight on the corrosion potential of methanogens, particularly in dynamic conditions, that offers new opportunities for monitoring and development of mitigation strategies. Overall, this study shows under certain conditions methanogenic archaea can cause higher corrosion than SRB, specific quantifications, i.e., maximum, average, and minimum corrosion rates can be determined, and that spatial statistical evaluations of MIC can be carried out. KW - Microbiologically influenced corrosion KW - Methanogen KW - Methane KW - Biocorrosion KW - Flow system KW - Modeling KW - Multiport PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-506084 DO - https://doi.org/10.3389/fmicb.2020.00527 VL - 11 SP - Article 527 PB - Frontiers in microbiology AN - OPUS4-50608 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Xu, Yong A1 - Huang, Yanliang A1 - Yang, D. A1 - Kunte, Hans-Jörg A1 - De Marco, R. A1 - Wang, X. T1 - Investigation of the calcareous deposits formation controlled by interfacial pH and its effect on the hydrogen entry into AISI 4135 steel in seawater N2 - The influence of interfacial pH between AISI 4135 steel and seawater under different polarization potentials on the formation of calcareous deposits has been studied. An interfacial pH of 9.61 at −0.9 V vs. SCE using state of the art iridium oxide microelectrode was found to be the critical pH for the precipitation of magnesium hydroxide. Calcareous deposits with a double-layer structure comprising an inner-brucite layer and an outer-aragonite layer were found to form at potentials between −1.0 V and −1.2 V vs. SCE. Furthermore, the facilitation of hydrogen permeation into steel induced by the formation of calcareous deposits was verified using the Devanathan-Stachurski electrochemical test. The mechanism of calcareous deposits facilitates hydrogen permeation into steel is related to its inhibition on hydrogen recombination and escape processes. KW - Low alloy steel KW - Cathodic protection KW - Calcareous deposits KW - Hydrogen permeation PY - 2020 DO - https://doi.org/10.1016/j.ijhydene.2020.11.040 SN - 0360-3199 SP - 1 EP - 18 PB - Elsevier Ltd. AN - OPUS4-51730 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Yalçin, M. A1 - Taşçioğlu, C. A1 - Plarre, Rüdiger A1 - Akçay, Ç. A1 - Busweiler, Sabine T1 - Investigation of natural durability of some native and exotic wood species against Hylotrupes bajulus (Cerambycidae) and Anobium punctatum (Anobiidae) N2 - Aim of study: In this study, natural durability of some domestic and foreign wood species against Hylotrupes bajulus and Anobium punctatum larvae were tested on laboratory scale Area of study: This study was conducted at Department of Forest Products Engineering in Duzce University, Turkey and Federal Institute for Materials Research and Testing (BAM), Germany. Material and Methods: Scotch pine (Pinus sylvestris), fir (Abies nordmanniana), spruce (Picea orientalis), cedar (Cedrus libani), poplar (Populus tremula) and beech (Fagus orientalis) woods were used to test H. bajulus larvae (EN 46-1). Alder (Alnus glutinosa), oak (Quercus cerris), poplar (Populus tremula), beech (Fagus orientalis), maple (Acer carpinifolium), ash (Fraxinus angustifolia), teak (Tectona grandis), ayous (Triplochiton scleroxylon), movingui (Distemonanthus benthamianus), dahoma (Piptadeniastrum africanum), iroko (Chlorophora excelsa), bubinga (Guibourtia tessmannii) and sapele (Entandrophragma cylindiricum) woods were used for A. punctatum larvae (EN 49-1). At the end of the experiment, the mortality rates of the larvae were determined and the size and weights of the surviving larvae were measured. Main results: F. orientalis and C. libani were found to be the most resistant wood species against H. bajulus larvae while A. nordmanniana was the least resistant. All tropical wood species and oak and maple from domestic wood species showed 100% mortality rate therefore found to be the most resistant against A. punctatum larvae. The most vulnerable wood species was found to be alder with a 35% mortality. Research highlights: while F. orientalis, C. libani, and P. tremula were found the most resistance wood species against H. bajulus, P. sylvestris and A. nordmanniana were determined as most vulnerable. All tropical wood species and two domestic species (Q. cerris and A. carpinifolium) showed the highest mortality rate as 100%. The least durable domestic wood was determined as alder. KW - Hylotrupes bajulus, Anobium punctatum, natural durability, tropical wood, native wood. PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-446572 DO - https://doi.org/10.17475/kastorman.311971 SN - 1303-2399 SN - 1309-4181 VL - 18 IS - 1 SP - 83 EP - 91 PB - Kastamonu University CY - Kastamonu AN - OPUS4-44657 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Oprzeska-Zingrebe, E. A. A1 - Meyer, Susann A1 - Roloff, Alexander A1 - Kunte, Hans-Jörg A1 - Smiatek, J. T1 - Influence of compatible solute ectoine on distinct DNA structures: thermodynamic insights into molecular binding mechanisms and destabilization effects N2 - In nature, the cellular environment of DNA includes not only water and ions, but also other components and co-solutes, which can exert both stabilizing and destabilizing effects on particular oligonucleotide conformations. Among them, ectoine, known as an important osmoprotectant organic co-solute in a broad range of pharmaceutical products, turns out to be of particular relevance. In this article, we study the influence of ectoine on a short single-stranded DNA fragment and on double-stranded helical B-DNA in aqueous solution by means of atomistic molecular dynamics (MD) simulations in combination with molecular theories of solution. Our results demonstrate a conformation-dependent binding behavior of ectoine, which favors the unfolded state of DNA by a combination of electrostatic and dispersion interactions. In conjunction with the Kirkwood–Buff theory, we introduce a simple Framework to compute the influence of ectoine on the DNA melting temperature. Our findings reveal a significant linear decrease of the melting temperature with increasing ectoine concentration, which is found to be in qualitative agreement with results from denaturation experiments. The outcomes of our Computer simulations provide a detailed mechanistic rationale for the surprising destabilizing influence of ectoine on distinct DNA structures. KW - Ectoine KW - DNA KW - Thermodynamic KW - Melting temperature PY - 2018 DO - https://doi.org/10.1039/c8cp03543a SN - 1463-9076 SN - 1463-9084 VL - 20 IS - 40 SP - 25861 EP - 25874 PB - Royal Society of Chemistry AN - OPUS4-46327 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Usmani, S. A1 - Voss, L. A1 - Stephan, Ina A1 - Hübert, Thomas A1 - Kemnitz, E. T1 - Improved Durability of Wood Treated with Nano Metal Fluorides against Brown-Rot and White-Rot Fungi N2 - Low-water soluble metal fluorides such as magnesium fluoride (MgF2) and calcium Fluoride (CaF2) were evaluated for decay protection of wood. Initially, the biocidal efficacy of nano metal fluorides (NMFs) against wood destroying fungi was assessed with an in-vitro agar test. The results from the test showed that agar medium containing MgF2 and CaF2 was more efficient in preventing fungal decay than stand-alone MgF2 or CaF2. These metal fluorides, in their nanoscopic form synthesized using fluorolytic sol-gel synthesis, were introduced into the sapwood of Scots pine and beech wood and then subjected to accelerated ageing by leaching (EN 84). MAS 19F NMR and X-ray micro CT images showed that metal fluorides were present in treated wood, unleached and leached. Decay resistance of Scots pine and beech wood treated with NMFs was tested against Wood destroying fungi Rhodonia placenta and Trametes versicolor in accordance with EN 113. Results revealed that mass losses were reduced to below 3% in wood treated with the combination of MgF2 and CaF2. It is concluded that NMFs provide full protection to wood even after it has been leached and can be used as wood preservatives in outdoor environments. KW - Nanoparticles KW - Fluoride KW - Wood protection KW - Fluorolytic sol-gel synthesis KW - Brown-rot fungi KW - White-rot fungi KW - Basidiomycetes PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-543877 DO - https://doi.org/10.3390/app12031727 VL - 12 IS - 3 SP - 1 EP - 11 PB - MDPI AN - OPUS4-54387 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Valentin, Jules D. P. A1 - Altenried, Stefanie A1 - Varadarajan, Adithi R. A1 - Ahrens, Christian H. A1 - Schreiber, Frank A1 - Webb, Jeremy S. A1 - van der Mei, Henny C. A1 - Ren, Qun T1 - Identification of Potential Antimicrobial Targets of Pseudomonas aeruginosa Biofilms through a Novel Screening Approach N2 - Pseudomonas aeruginosa is an opportunistic pathogen of considerable medical importance, owing to its pronounced antibiotic tolerance and association with cystic fibrosis and other life-threatening diseases. The aim of this study was to highlight the genes responsible for P. aeruginosa biofilm tolerance to antibiotics and thereby identify potential new targets for the development of drugs against biofilm-related infections. By developing a novel screening approach and utilizing a public P. aeruginosa transposon insertion library, several biofilm-relevant genes were identified. The Pf phage gene (PA0720) and flagellin gene (fliC) conferred biofilm-specific tolerance to gentamicin. Compared with the reference biofilms, the biofilms formed by PA0720 and fliC mutants were completely eliminated with a 4-fold-lower gentamicin concentration. Furthermore, the mreC, pprB, coxC, and PA3785 genes were demonstrated to play major roles in enhancing biofilm tolerance to gentamicin. The analysis of biofilm-relevant genes performed in this study provides important novel insights into the understanding of P. aeruginosa antibiotic tolerance, which will facilitate the detection of antibiotic resistance and the development of antibiofilm strategies against P. aeruginosa. KW - Antimicrobial resistance KW - Bacteria KW - Biofilms KW - Pseudomonas aeruginosa PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-570205 DO - https://doi.org/10.1128/spectrum.03099-22 SP - 1 EP - 5 PB - ASM Journals AN - OPUS4-57020 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Huang, Yanliang A1 - Liu, Xiangju A1 - Zhang, Qichao A1 - Xu, Yong A1 - Kunte, Hans-Jörg A1 - De Marco, Roland T1 - Hydrogen release from carbon steel in chloride solution under anodic polarization N2 - The hydrogen permeation current increase was noticed for carbon steel in 0.5 mol/L NaCl solution under strong anodic potentials, which is contrary to the common understanding. Hydrogen permeation under cathodic potentials has been widely studied because of possible hydrogen embrittlement failures of high strength steels in seawater, but investigations of anodic polarization on hydrogen permeation are fairly rare, as the hydrogen evolution reaction shall be retarded. To corroborate the observed phenomenon, experiments were conducted using both as-received and vacuum-annealed sheet specimens. It was verified that the observed phenomena originated from the released hydrogen in traps by metal dissolution under anodic polarization. KW - Energy Engineering and Power Technology KW - Condensed Matter Physics KW - Fuel Technology KW - Renewable Energy, Sustainability and the Environment PY - 2020 DO - https://doi.org/10.1016/j.ijhydene.2019.11.218 SN - 1879-3487 VL - 45 IS - 4 SP - 3307 EP - 3315 PB - Elsevier BV CY - New York, NY AN - OPUS4-59322 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Oberpaul, M. A1 - Zumkeller, C. M. A1 - Culver, T. A1 - Spohn, M. A1 - Mihajlovic1, S. A1 - Leis, B. A1 - Glaeser, S. P. A1 - Plarre, Rüdiger A1 - McMahon, Dino Peter A1 - Hammann, P. A1 - Schäberle, T. F. A1 - Glaeser, J. A1 - Vilcinskas, A. T1 - High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests N2 - Microbial communities in the immediate environment of socialized invertebrates can help to suppress pathogens, in part by synthesizing bioactive natural products. Here we characterized the core microbiomes of three termite species (genus Coptotermes) and their nest material to gain more insight into the diversity of termite-associated bacteria. Sampling a healthy termite colony over time implicated a consolidated and highly stable microbiome, pointing toward the fact that beneficial bacterial phyla play a major role in termite fitness. In contrast, there was a significant shift in the composition of the core microbiome in one nest during a fungal infection, affecting the abundance of wellcharacterized Streptomyces species (phylum Actinobacteria) as well as less-studied bacterial phyla such as Acidobacteria. High-throughput cultivation in microplates was implemented to isolate and identify these less-studied bacterial phylogenetic group. Amplicon sequencing confirmed that our method maintained the bacterial diversity of the environmental samples, enabling the isolation of novel Acidobacteriaceae and expanding the list of cultivated species to include two strains that may define new species within the genera Terracidiphilus and Acidobacterium. KW - Termite-associated microbes KW - Termites KW - Coptotermes KW - Core microbiome KW - Natural products discovery KW - Acidobacteria KW - underexplored phyla KW - Social insects PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-515283 DO - https://doi.org/10.3389/fmicb.2020.597628 VL - 11 SP - 1 EP - 16 AN - OPUS4-51528 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, Beate A1 - Pfeiffer, F. A1 - Dyall-Smith, M. A1 - Kunte, Hans-Jörg T1 - Genome Sequence of Pseudomonas veronii Strain G2, a Member of a Bacterial Consortium Capable of Polyethylene Degradation N2 - Nine different bacterial isolates were recovered from landfills. Each isolate was obtained in pure culture. As a consortium, the bacteria degrade polyethylene. The complete genome sequence of strain G2 was determined by PacBio sequencing. Using the TYGS server for taxonomic classification, strain G2 was assigned to the species Pseudomonas veronii. KW - Polyethylene KW - Next generation sequencing KW - Pseudomonas veronii PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-548973 DO - https://doi.org/10.1128/mra.00365-22 SP - 1 EP - 2 PB - ASM AN - OPUS4-54897 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -